FastQCFastQC Report
Thu 26 May 2016
SRR1364436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748577
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21400.048.6731721
GGTATCA13750.043.720391
ATCAACG22750.043.399173
CAACGCA22750.043.141435
TCAACGC22800.043.043134
AACGCAG24000.040.894486
GTACATG61050.040.3626821
TATCAAC26550.037.859682
TACATGG65300.036.980062
ACATGGG64850.036.044363
ACGCAGA30050.032.8590977
CGCAGAG30500.032.374298
CATGGGG35250.029.3592434
CATGGGA37600.026.4169924
GCAGAGT39450.023.672539
GAGTACA44850.023.622341
TAAGGTA6100.022.4260464
GTGTAGG10750.022.14711
AGGACCG5250.021.5271385
ATGGGGA23500.020.755735
Produced by FastQC (version 0.11.5)