##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364436_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1748577 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.50095134500797 31.0 30.0 34.0 25.0 34.0 2 29.49498077579655 31.0 30.0 34.0 25.0 34.0 3 29.445314103982838 31.0 30.0 34.0 23.0 34.0 4 32.95878763131392 35.0 33.0 37.0 26.0 37.0 5 33.11451311552194 35.0 33.0 37.0 27.0 37.0 6 33.258463882345474 37.0 35.0 37.0 27.0 37.0 7 33.06648949402857 36.0 33.0 37.0 26.0 37.0 8 32.931420234853825 36.0 33.0 37.0 26.0 37.0 9 33.93807250123958 38.0 34.0 39.0 23.0 39.0 10-11 34.07687107859705 38.0 33.5 39.0 24.5 39.0 12-13 34.12016371026269 38.0 34.0 39.0 25.0 39.0 14-15 35.10719745255713 39.0 34.0 41.0 25.0 41.0 16-17 35.22550736970691 39.0 34.0 41.0 25.0 41.0 18-19 35.1720313146061 39.0 34.0 41.0 25.0 41.0 20-21 35.11994238743847 39.0 34.0 41.0 24.5 41.0 22-23 35.00874396723736 39.0 34.0 41.0 24.0 41.0 24-25 35.04042286956766 39.0 34.0 41.0 24.5 41.0 26-27 35.029466531928534 39.0 34.0 41.0 24.5 41.0 28-29 34.96444909203312 39.0 34.0 41.0 24.5 41.0 30-31 34.73495219255429 39.0 34.0 41.0 24.0 41.0 32-33 34.795400202564714 39.0 34.0 41.0 24.0 41.0 34-35 34.72767999350329 39.0 34.0 41.0 24.0 41.0 36-37 34.59209031115015 39.0 33.5 41.0 23.5 41.0 38-39 34.4362049826802 39.0 33.0 41.0 22.5 41.0 40-41 34.40104610777792 39.0 33.0 41.0 22.5 41.0 42-43 34.239625993021754 38.0 33.0 40.5 22.0 41.0 44-45 34.21211219179939 38.0 33.0 40.0 22.0 41.0 46-47 34.057882495309045 38.0 33.0 40.0 21.5 41.0 48-49 33.886291252830155 38.0 33.0 40.0 20.0 41.0 50-51 33.729383092651915 38.0 33.0 40.0 19.0 41.0 52-53 33.58641340930368 38.0 32.5 40.0 19.0 41.0 54-55 33.44498640894854 38.0 32.0 40.0 18.5 41.0 56-57 33.40649711165136 38.0 32.5 40.0 18.5 41.0 58-59 33.399234634791604 38.0 32.0 40.0 18.0 41.0 60-61 33.21856829867944 37.0 32.0 40.0 18.0 41.0 62-63 32.96869711771343 37.0 32.0 40.0 15.0 41.0 64-65 32.67438837408933 36.5 32.0 40.0 13.0 41.0 66-67 32.3802471952908 36.0 31.0 39.0 12.5 41.0 68-69 32.099335059308224 35.5 31.0 39.0 9.5 41.0 70-71 31.75412206611433 35.0 31.0 39.0 8.0 40.5 72-73 31.4362910526674 35.0 31.0 38.0 7.0 40.0 74-75 31.084807532067504 35.0 31.0 37.0 4.5 39.5 76-77 30.74294983864022 35.0 30.0 37.0 2.0 39.0 78-79 30.428017753865 35.0 30.0 36.0 2.0 39.0 80-81 30.106089408702047 35.0 30.0 36.0 2.0 37.5 82-83 29.815580040226997 34.0 30.0 35.5 2.0 37.0 84-85 29.490312408318307 34.0 29.5 35.0 2.0 37.0 86-87 29.290441885029942 34.0 29.0 35.0 2.0 36.0 88-89 29.13898329899112 34.0 29.0 35.0 2.0 36.0 90-91 29.008581549454213 34.0 29.0 35.0 2.0 36.0 92-93 28.784609714070356 34.0 29.0 35.0 2.0 35.0 94-95 28.701333141177084 34.0 29.0 35.0 2.0 35.0 96-97 28.626013895870756 34.0 29.0 35.0 2.0 35.0 98-99 28.479235687075835 34.0 29.0 35.0 2.0 35.0 100-101 28.357517284054406 34.0 29.0 35.0 2.0 35.0 102-103 27.334871155230797 32.5 26.0 34.0 2.0 35.0 104-105 28.2777169664247 34.0 29.0 35.0 2.0 35.0 106-107 28.535965816775587 34.0 29.0 35.0 2.0 35.0 108-109 28.538085826360522 34.0 29.0 35.0 2.0 35.0 110-111 28.44062886564332 34.0 29.0 35.0 2.0 35.0 112-113 28.32600222924126 34.0 29.0 35.0 2.0 35.0 114-115 28.230614379578366 34.0 29.0 35.0 2.0 35.0 116-117 28.11101541424827 34.0 29.0 35.0 2.0 35.0 118-119 27.855022398212945 34.0 29.0 35.0 2.0 35.0 120-121 27.712046138088283 34.0 29.0 35.0 2.0 35.0 122-123 27.468608760151827 34.0 28.5 35.0 2.0 35.0 124-125 26.28576922834968 33.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7421416968912098 1101 2 0.7479178343800044 1101 3 0.9226871677045985 1101 4 0.8425614698317361 1101 5 0.7460696186741842 1101 6 0.6503330762663566 1101 7 0.7671303048767584 1101 8 0.8908201695638098 1101 9 1.407127709251533 1101 10-11 1.2661787401792424 1101 12-13 1.0688209764928942 1101 14-15 1.342570679188242 1101 16-17 1.1028849255307236 1101 18-19 1.1001216272044658 1101 20-21 1.1653024189954948 1101 22-23 1.112216670861244 1101 24-25 1.2132738062008173 1101 26-27 1.1979838097624338 1101 28-29 1.1505923688490682 1101 30-31 1.2708831543959178 1101 32-33 1.1461017681135175 1101 34-35 1.1587884842373484 1101 36-37 1.0821077625742603 1101 38-39 1.2968198712241659 1101 40-41 1.2815695445898214 1101 42-43 1.3817402127478005 1101 44-45 1.1703282200638938 1101 46-47 1.1682013126742774 1101 48-49 1.0517112823140522 1101 50-51 1.0476691487515524 1101 52-53 0.9777071003559357 1101 54-55 0.8899919935990965 1101 56-57 0.9587452546948683 1101 58-59 1.1062547603884525 1101 60-61 1.0579093119836642 1101 62-63 1.1291900137397803 1101 64-65 0.977516357366035 1101 66-67 1.0015766889482975 1101 68-69 0.8599984999745232 1101 70-71 0.7062107957882091 1101 72-73 0.7475715496370974 1101 74-75 0.8202735202036671 1101 76-77 0.9748946763606767 1101 78-79 0.9163990228049634 1101 80-81 0.9292669548852892 1101 82-83 1.0042256038622348 1101 84-85 1.1475306304251802 1101 86-87 1.099328038082568 1101 88-89 0.9893355627711493 1101 90-91 1.071332252607096 1101 92-93 1.132123804038784 1101 94-95 1.0810111870754646 1101 96-97 1.1300966487605244 1101 98-99 1.1017895227168673 1101 100-101 0.9709449757054891 1101 102-103 0.9061646167964632 1101 104-105 0.9869704284184699 1101 106-107 0.8992273694966073 1101 108-109 0.9960723985283693 1101 110-111 1.1522947872324245 1101 112-113 1.0231717178175295 1101 114-115 1.058987619278735 1101 116-117 0.991855845169983 1101 118-119 0.9980359682677218 1101 120-121 0.913487752709802 1101 122-123 0.9027154696537707 1101 124-125 0.6071022082690973 1102 1 0.33124827027575066 1102 2 0.340906940545711 1102 3 0.4866725921180226 1102 4 0.35898972603929735 1102 5 0.3939265453173775 1102 6 0.39831569819755686 1102 7 0.5116625307287563 1102 8 0.4522453878561734 1102 9 0.8663196922421221 1102 10-11 0.669190549537646 1102 12-13 0.4984147985687599 1102 14-15 0.6931679320043784 1102 16-17 0.5521660833076538 1102 18-19 0.49757608286437716 1102 20-21 0.6476070908038309 1102 22-23 0.7510172177878971 1102 24-25 0.7415050144786193 1102 26-27 0.6538140662329539 1102 28-29 0.5722391270885296 1102 30-31 0.5775000146869047 1102 32-33 0.5663346909606659 1102 34-35 0.4182472621378679 1102 36-37 0.43727062409382 1102 38-39 0.6099272995638003 1102 40-41 0.41702336165418075 1102 42-43 0.5930247754940297 1102 44-45 0.44197105442056994 1102 46-47 0.46435210741704935 1102 48-49 0.43313634918386157 1102 50-51 0.3160834793247673 1102 52-53 0.25839626812442873 1102 54-55 0.1850631017807629 1102 56-57 0.25587345296873565 1102 58-59 0.404415471270795 1102 60-61 0.3156826402676387 1102 62-63 0.27803985016701205 1102 64-65 0.4051248694689846 1102 66-67 0.33014610825878066 1102 68-69 0.3695782339279958 1102 70-71 0.4209870318222073 1102 72-73 0.3962655955417205 1102 74-75 0.31393663149764706 1102 76-77 0.33552658601615093 1102 78-79 0.17810358660824477 1102 80-81 0.41972092701218244 1102 82-83 0.32647953746373304 1102 84-85 0.5755871494796025 1102 86-87 0.4704442131136588 1102 88-89 0.30774248965458284 1102 90-91 0.4346204634723385 1102 92-93 0.2575646584580724 1102 94-95 0.26886092405883844 1102 96-97 0.28678172928457 1102 98-99 0.3048174789621534 1102 100-101 0.27733171737873974 1102 102-103 0.18587266826907367 1102 104-105 0.2560151282137717 1102 106-107 0.3232819649020655 1102 108-109 0.33929610046936176 1102 110-111 0.27484114110084334 1102 112-113 0.3513320778072 1102 114-115 0.14103113084370733 1102 116-117 0.12846644326436163 1102 118-119 0.24043874151374212 1102 120-121 0.16639568473329902 1102 122-123 0.19723839903392815 1102 124-125 -0.03554723815767247 1103 1 0.7285389094347607 1103 2 0.7088593752239838 1103 3 0.8556333543093224 1103 4 0.8105397896149285 1103 5 0.6967130565371349 1103 6 0.6826799597351894 1103 7 0.6778263946947973 1103 8 0.7981527286036822 1103 9 1.2177324324416219 1103 10-11 1.0485775013097083 1103 12-13 1.0033281387073743 1103 14-15 1.1675230601406241 1103 16-17 0.9994253823638672 1103 18-19 1.0762114452455123 1103 20-21 1.0805095424953066 1103 22-23 1.0942716456967077 1103 24-25 1.0387465123564539 1103 26-27 1.1677693304747905 1103 28-29 1.09046538472208 1103 30-31 1.3122784312058258 1103 32-33 1.1401056395808098 1103 34-35 1.198015739367051 1103 36-37 1.1319567753501119 1103 38-39 1.1904149157460324 1103 40-41 1.0772598272069231 1103 42-43 1.175062705972735 1103 44-45 0.9270777361304852 1103 46-47 0.8750460668360986 1103 48-49 0.995449571125512 1103 50-51 0.9907818084495759 1103 52-53 0.9281321874639517 1103 54-55 0.8836458844237214 1103 56-57 0.9727336402930078 1103 58-59 0.9979589802877911 1103 60-61 1.0101880576282554 1103 62-63 1.0368353873363745 1103 64-65 0.9779948707225898 1103 66-67 0.8283083962653706 1103 68-69 0.7909501066334457 1103 70-71 0.9925104473561532 1103 72-73 0.9209141744919158 1103 74-75 0.9612274497429567 1103 76-77 0.8981761320323756 1103 78-79 0.9003370793283842 1103 80-81 0.9449758205453733 1103 82-83 0.9083943998359061 1103 84-85 1.0109065498986638 1103 86-87 0.9872955177573672 1103 88-89 1.0012054814703362 1103 90-91 1.106466592521926 1103 92-93 1.0103893866946088 1103 94-95 0.9923329060069399 1103 96-97 1.0285341245638548 1103 98-99 1.0428208816018838 1103 100-101 1.0103317352873695 1103 102-103 0.8334089063822177 1103 104-105 0.9779096463820771 1103 106-107 1.028884357495059 1103 108-109 1.0119748375527635 1103 110-111 1.046343567720232 1103 112-113 1.1187721823936023 1103 114-115 1.1785989239632144 1103 116-117 1.0303490701022362 1103 118-119 0.9517254765913776 1103 120-121 0.7952283644016305 1103 122-123 0.9375261549034803 1103 124-125 0.8553437878277492 1104 1 0.5413624490882825 1104 2 0.5620004912682077 1104 3 0.7556942927614863 1104 4 0.5487280023771817 1104 5 0.6463632698217197 1104 6 0.5453135455278257 1104 7 0.7237746840653898 1104 8 0.8800923124811106 1104 9 1.1998088666716171 1104 10-11 0.985270806785465 1104 12-13 0.8379544118857467 1104 14-15 0.880404752488495 1104 16-17 0.7406462407254892 1104 18-19 0.8756838923596391 1104 20-21 0.9912242634842485 1104 22-23 0.9374968568049198 1104 24-25 1.1266681629179516 1104 26-27 1.106066187791754 1104 28-29 1.0351691367896336 1104 30-31 0.9983378026692264 1104 32-33 1.0175089650791946 1104 34-35 0.9996666490910826 1104 36-37 0.8034079810609143 1104 38-39 1.012032048156584 1104 40-41 0.9433038770023501 1104 42-43 0.9049984594331661 1104 44-45 0.7941295338148251 1104 46-47 0.8162561303862006 1104 48-49 0.7421750869259824 1104 50-51 0.7564177447253684 1104 52-53 0.5850946667884074 1104 54-55 0.6052344628166892 1104 56-57 0.5902362084134936 1104 58-59 0.7488941243637015 1104 60-61 0.7591299919888428 1104 62-63 0.7025294763065162 1104 64-65 0.6636304587786555 1104 66-67 0.6413690735527382 1104 68-69 0.6097041764515012 1104 70-71 0.6797683911186709 1104 72-73 0.6798402648549349 1104 74-75 0.6278787947416546 1104 76-77 0.6735566491386678 1104 78-79 0.4892490667924747 1104 80-81 0.6483653913645604 1104 82-83 0.6942608575707396 1104 84-85 0.6668741654640833 1104 86-87 0.6805330906658682 1104 88-89 0.6845048633803223 1104 90-91 0.7354882691999087 1104 92-93 0.7760340284635632 1104 94-95 0.7175687378168156 1104 96-97 0.6758923556076439 1104 98-99 0.6583535707287247 1104 100-101 0.6778424865714321 1104 102-103 0.655337347228393 1104 104-105 0.6609301042910225 1104 106-107 0.6124348058279558 1104 108-109 0.5784357063609775 1104 110-111 0.6492606043703582 1104 112-113 0.7510169808614364 1104 114-115 0.7366137233015948 1104 116-117 0.769367786912543 1104 118-119 0.6644337555153754 1104 120-121 0.5776429199862072 1104 122-123 0.537367366708132 1104 124-125 0.2633692078677967 1105 1 0.4566407534487702 1105 2 0.49978808773373373 1105 3 0.5847840456071047 1105 4 0.4329756142759251 1105 5 0.38421764582492557 1105 6 0.5089732426112548 1105 7 0.5836508233160203 1105 8 0.7306840569244812 1105 9 1.228033416690323 1105 10-11 0.6837779527694821 1105 12-13 0.8627200622247742 1105 14-15 0.45384223556262526 1105 16-17 0.6573401693312775 1105 18-19 0.6755870365416285 1105 20-21 0.9410227460339513 1105 22-23 0.9850093508868341 1105 24-25 0.9488597424515604 1105 26-27 0.8799244201858158 1105 28-29 0.7650894282746705 1105 30-31 0.9786584209152025 1105 32-33 0.8885904941012157 1105 34-35 0.8050968195509469 1105 36-37 0.8277147132411997 1105 38-39 1.0045534408667862 1105 40-41 0.8717078014471653 1105 42-43 0.9136898035179257 1105 44-45 0.7939377338067999 1105 46-47 0.8554799329583531 1105 48-49 0.758324838764814 1105 50-51 0.819912914360053 1105 52-53 0.7543476599199295 1105 54-55 0.8050499907159363 1105 56-57 0.7587318145365671 1105 58-59 0.8735699281873508 1105 60-61 0.889062263009265 1105 62-63 0.8617230369239195 1105 64-65 0.7927103348796791 1105 66-67 0.867041918813463 1105 68-69 0.8177232646941661 1105 70-71 0.8422417955943047 1105 72-73 0.8216389666046489 1105 74-75 0.8579767401876133 1105 76-77 0.7605305611515298 1105 78-79 0.7280265679506002 1105 80-81 0.7031588941233586 1105 82-83 0.6933467807882892 1105 84-85 0.8099604761049051 1105 86-87 0.739750164307516 1105 88-89 0.681192203241654 1105 90-91 0.7294436377840867 1105 92-93 0.7639756702843776 1105 94-95 0.7542736519114364 1105 96-97 0.8687224762377426 1105 98-99 0.8267417433589266 1105 100-101 0.6984226911538585 1105 102-103 0.5810774817942566 1105 104-105 0.7149129493414641 1105 106-107 0.6548248378349797 1105 108-109 0.6438072663067658 1105 110-111 0.7473596177791499 1105 112-113 0.8284468989864102 1105 114-115 0.7672664869103158 1105 116-117 0.6779733626216107 1105 118-119 0.5432516430007794 1105 120-121 0.5106741311363479 1105 122-123 0.6026202145559267 1105 124-125 0.4705967546819707 1106 1 0.6358352437958459 1106 2 0.6501865420619417 1106 3 0.6950817955563267 1106 4 0.36448497632270715 1106 5 0.42491929154912356 1106 6 0.38379815089654556 1106 7 0.45473492501339763 1106 8 0.7368748937287251 1106 9 1.187685841143228 1106 10-11 0.599657323166511 1106 12-13 0.8541585600845565 1106 14-15 1.0941037384551109 1106 16-17 0.8031845464611536 1106 18-19 0.8985752684726407 1106 20-21 0.8989439378361794 1106 22-23 0.9923398197289117 1106 24-25 0.9952203047511077 1106 26-27 0.9444452468845199 1106 28-29 0.8999357512859731 1106 30-31 0.9130639742000071 1106 32-33 1.0508829605811485 1106 34-35 0.9958978907887683 1106 36-37 0.9976368027567517 1106 38-39 1.0853676392815004 1106 40-41 0.9864413962374172 1106 42-43 0.9544953883982217 1106 44-45 0.619785170183242 1106 46-47 0.7264112603955368 1106 48-49 0.8186448434790563 1106 50-51 0.7988907498678728 1106 52-53 0.819442910314244 1106 54-55 0.7429148121386859 1106 56-57 0.6252427187445306 1106 58-59 0.7929072502432604 1106 60-61 0.7699009730102375 1106 62-63 0.8253797518447712 1106 64-65 0.8841363575331442 1106 66-67 0.7927434767723938 1106 68-69 0.8592392467499224 1106 70-71 0.8005984912067916 1106 72-73 0.8359820059410445 1106 74-75 0.8170815093524162 1106 76-77 0.8550456078802675 1106 78-79 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scores pass #Quality Count 2 62541.0 3 10961.0 4 6869.0 5 5893.0 6 6055.0 7 6780.0 8 7539.0 9 8041.0 10 8711.0 11 9050.0 12 9640.0 13 9795.0 14 9891.0 15 9428.0 16 8801.0 17 8336.0 18 7714.0 19 7313.0 20 7363.0 21 7816.0 22 9138.0 23 11919.0 24 16327.0 25 21703.0 26 25807.0 27 28848.0 28 34079.0 29 39885.0 30 47022.0 31 55225.0 32 67388.0 33 85632.0 34 113511.0 35 162324.0 36 275875.0 37 402357.0 38 132783.0 39 217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.321826421496766 18.428441326426935 12.444740320290055 24.804991931786244 2 15.68377498119887 20.078979048534084 39.32132920423319 24.915916766033863 3 18.828624647356108 24.460232520500956 29.21981702836078 27.491325803782164 4 12.40809454309205 16.179649718392987 37.14473136383809 34.267524374676874 5 13.401515307488006 38.550037859811184 34.58682478570889 13.461622046991916 6 32.8740635935703 36.63987720272473 17.207288457502205 13.278770746202762 7 28.855480116391853 32.41603453324305 20.986391212049117 17.742094138315977 8 26.317083771789356 35.763828521755045 19.76780893992771 18.151278766527888 9 26.377512125629586 16.090185746173013 19.63611387368757 37.89618825450983 10-11 24.800111186767175 26.70467073074128 28.01102945276836 20.484188629723185 12-13 25.208338277938974 24.080911000815824 27.877081345363568 22.83366937588163 14-15 22.28225746492728 25.76894041212232 24.911496073799977 27.037306049150416 16-17 22.795767984336543 29.02389066006073 25.794775435459016 22.38556592014371 18-19 22.815392376566194 27.905860739235827 27.052425549633767 22.22632133456421 20-21 23.18385433483757 27.252044648365096 27.03863532430524 22.525465692492094 22-23 23.09035949908631 27.10413205178434 26.920135780897446 22.885372668231902 24-25 23.027394220309105 27.329360934981622 26.92061532900404 22.722629515705233 26-27 22.720445710108837 27.684409926555748 26.606968309901447 22.988176053433968 28-29 22.73575687245006 27.69532531269866 26.828728441243765 22.740189373607517 30-31 22.844205908791 27.19996659534812 26.885453789897895 23.070373705962986 32-33 22.50220183696112 27.499084950873296 26.787319706726752 23.211393505438824 34-35 23.231098557267508 27.460214336735948 26.533335773440612 22.77535133255593 36-37 23.300338734130595 27.030160090908623 26.590228924337556 23.079272250623223 38-39 22.774828388634617 27.216164266519044 26.826675237465064 23.182332107381278 40-41 23.068953852427164 27.05321856037004 26.887710327422067 22.990117259780725 42-43 22.930133923848075 26.96883841350253 26.846278390260164 23.254749272389223 44-45 22.785193081625405 27.24723358235715 26.849249182613587 23.118324153403854 46-47 23.07426812909786 27.491558002790313 26.348782233502682 23.08539163460914 48-49 22.958668345875928 27.25941152180055 26.61049093993395 23.171429192389567 50-51 23.005575942947456 27.274565218572445 26.609850136703393 23.110008701776703 52-53 23.18738418247123 27.11385578000961 26.517438402232845 23.181321635286313 54-55 22.72398016392416 27.0739385980741 26.837526570258298 23.36455466774344 56-57 22.723031197872118 27.487775283122414 26.79926184129588 22.989931677709592 58-59 22.914436086441274 26.96557013630561 26.69112297703532 23.428870800217798 60-61 22.687433546119205 27.130856735299215 27.058418787995603 23.12329093058597 62-63 22.7152496101294 27.18607087365619 26.997863397578215 23.1008161186362 64-65 22.613032705875895 27.145322208698996 26.7181966470529 23.52344843837221 66-67 22.415016918828517 27.420770922036112 26.671577969603305 23.492634189532062 68-69 22.637668609263493 26.979283860698132 27.142866948037202 23.240180582001173 70-71 22.606238629494086 26.91443431572217 26.895274959636673 23.58405209514708 72-73 22.561232471047827 26.98258222360129 26.836473661708204 23.619711643642678 74-75 22.5472097683489 27.228334526369437 26.799470698070543 23.424985007211124 76-77 22.66755048548076 26.980999992564943 26.90933746188817 23.442112060066126 78-79 22.767017361240192 26.981014450065345 26.951445149519426 23.300523039175033 80-81 22.674201454526735 27.26903024877955 26.854836172701457 23.201932123992254 82-83 22.69444683569247 27.21536418643879 26.88460930169107 23.205579676177667 84-85 22.698063068112923 27.092365752042518 26.858628681509007 23.350942498335556 86-87 22.766582555931063 26.893671274882518 26.98556103805403 23.354185131132382 88-89 22.737259051650174 26.93081850940914 26.885889149459473 23.44603328948121 90-91 22.58887959447769 27.184208080418102 26.970322230086722 23.256590095017483 92-93 22.700399159699913 26.647355931602362 27.14257362205828 23.509671286639445 94-95 22.482568737027712 26.596123742378204 27.249277784355076 23.67202973623901 96-97 22.779353812892857 26.542086810301928 27.117266545032344 23.561292831772867 98-99 22.581374839000443 26.65054573988877 26.950496782296568 23.81758263881422 100-101 22.362172478936444 26.523150608070583 27.319393342137577 23.795283570855393 102-103 22.397543099146265 26.319856392898235 27.385842277557842 23.896758230397655 104-105 22.203519247762934 26.457387817900518 27.36822839363184 23.97086454070471 106-107 22.355477151239086 26.56477473517811 27.16306359814617 23.916684515436636 108-109 22.379403310468206 26.501732328136924 26.98648681108644 24.132377550308433 110-111 22.261930328716208 26.64792057666961 27.223200388228797 23.866948706385386 112-113 22.3082363148703 26.661305640544914 27.434002613214265 23.596455431370526 114-115 22.108320494961077 26.775034253930656 27.209360653351368 23.9072845977569 116-117 21.99963566345713 26.775761725044877 27.354410516914623 23.870192094583366 118-119 21.896377612309838 26.92421435841946 27.04270822826017 24.136699801010536 120-121 22.00271606689716 26.96648849389857 27.15163066981825 23.879164769386012 122-123 22.029291844419433 26.932736875374513 27.19227617452688 23.845695105679173 124-125 21.96661816049343 26.956782690719688 27.15357807310779 23.923021075679088 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 484.0 1 391.5 2 265.0 3 208.0 4 164.0 5 138.5 6 135.0 7 135.0 8 124.0 9 118.5 10 117.0 11 112.0 12 118.0 13 135.0 14 167.5 15 195.0 16 221.0 17 251.0 18 313.0 19 420.5 20 580.5 21 873.5 22 1267.0 23 1809.0 24 2651.0 25 3922.5 26 5769.0 27 8006.0 28 10462.0 29 13401.0 30 16827.5 31 20757.0 32 25544.0 33 29134.5 34 31995.5 35 35774.5 36 40507.5 37 45090.5 38 48531.5 39 51230.5 40 53703.5 41 57802.0 42 61045.5 43 63686.5 44 66738.5 45 69764.5 46 70232.5 47 69535.0 48 70004.5 49 68629.0 50 67484.0 51 65989.0 52 64330.5 53 63755.0 54 62676.5 55 58351.5 56 52059.0 57 46840.5 58 42581.5 59 37236.5 60 30126.0 61 24212.5 62 19992.5 63 15484.0 64 11651.5 65 9242.0 66 7004.0 67 5659.0 68 4330.5 69 2853.0 70 1987.5 71 1441.5 72 1114.5 73 835.5 74 674.5 75 531.5 76 345.5 77 199.0 78 105.5 79 68.0 80 44.5 81 29.5 82 20.5 83 10.5 84 10.5 85 12.0 86 9.0 87 4.5 88 3.5 89 3.5 90 2.0 91 2.0 92 3.0 93 4.0 94 2.5 95 1.0 96 1.0 97 1.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09138859769972955 2 6.862723231519115E-4 3 0.0 4 0.002344763770769031 5 0.0012009765655158452 6 2.2875744105063715E-4 7 5.718936026265929E-5 8 9.722191244652081E-4 9 4.575148821012743E-4 10-11 0.009579217843995432 12-13 0.002201790370112383 14-15 0.003831687137598173 16-17 0.002773683972738976 18-19 0.013210742220674298 20-21 0.016785077237090502 22-23 0.009836569965177398 24-25 0.010236895487016014 26-27 0.010065327406228036 28-29 0.0073488327937517185 30-31 0.018157621883394325 32-33 0.002116006329718394 34-35 0.0011437872052531858 36-37 4.575148821012743E-4 38-39 2.2875744105063715E-4 40-41 0.0013439499661724932 42-43 0.0026307105720823273 44-45 9.150297642025486E-4 46-47 0.0015727074072231307 48-49 0.0077205636354590055 50-51 0.004775311581932051 52-53 0.007834942355984323 54-55 0.008378241278479586 56-57 0.009865164645308728 58-59 0.00874997212018687 60-61 0.01052284228832931 62-63 0.013754041143169561 64-65 0.008292457238085598 66-67 0.01309636350014898 68-69 0.013153552860411638 70-71 0.004803906262063381 72-73 0.012553064577653714 74-75 0.013525283702118925 76-77 0.005947693467316566 78-79 0.00812088915729762 80-81 0.013839825183563548 82-83 0.017356970839717097 84-85 0.014011393264351527 86-87 0.016384751715251885 88-89 0.015841452792756627 90-91 0.012267117776340418 92-93 0.008807161480449532 94-95 0.007892131716246984 96-97 0.009550623163864102 98-99 0.0071486700328324115 100-101 0.010179706126753355 102-103 0.011180519931349893 104-105 0.010980357170430584 106-107 0.005175637103770666 108-109 0.0036029296965475353 110-111 0.007062885992438423 112-113 0.00889294552084352 114-115 0.013582473062381584 116-117 0.011066141210824575 118-119 0.2150891839478616 120-121 0.026364295081085933 122-123 0.027422298245945134 124-125 0.23593470576360093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1748577.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.52287314502887 #Duplication Level Percentage of deduplicated Percentage of total 1 88.60048341501961 69.5716451978582 2 7.393814990177375 11.61167193063022 3 1.9466504638489228 4.585697622915618 4 0.7596759679371687 2.386077586466293 5 0.38984377773395074 1.5305826752690923 6 0.2098457834616099 0.9886616300865116 7 0.14368736702894694 0.7897921425630043 8 0.09675682178650129 0.6078098914446085 9 0.07087374350829574 0.5008688973733698 >10 0.35572111288286845 5.0309615841354445 >50 0.023245981630099503 1.270009619720372 >100 0.0092662811669718 1.045972733466019 >500 1.3429381766718313E-4 0.08024848807128654 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.7189360262659295E-5 0.0 3 0.0 0.0 0.0 5.7189360262659295E-5 0.0 4 0.0 0.0 0.0 5.7189360262659295E-5 0.0 5 0.0 0.0 0.0 5.7189360262659295E-5 0.0 6 0.0 0.0 0.0 5.7189360262659295E-5 0.0 7 0.0 0.0 0.0 5.7189360262659295E-5 0.0 8 0.0 0.0 0.0 5.7189360262659295E-5 0.0 9 0.0 0.0 0.0 1.1437872052531859E-4 0.0 10-11 0.0 0.0 0.0 1.1437872052531859E-4 0.0 12-13 0.0 0.0 0.0 1.7156808078797788E-4 0.0 14-15 0.0 0.0 0.0 1.7156808078797788E-4 0.0 16-17 0.0 0.0 0.0 2.2875744105063715E-4 0.0 18-19 0.0 0.0 0.0 3.4313616157595576E-4 0.0 20-21 0.0 0.0 0.0 3.4313616157595576E-4 0.0 22-23 0.0 0.0 0.0 5.432989224952633E-4 0.0 24-25 0.0 2.8594680131329647E-5 0.0 8.292457238085597E-4 0.0 26-27 0.0 5.7189360262659295E-5 0.0 0.0011723818853845154 0.0 28-29 0.0 5.7189360262659295E-5 0.0 0.003345577575365569 0.0 30-31 0.0 5.7189360262659295E-5 0.0 0.010208300806884684 0.0 32-33 0.0 5.7189360262659295E-5 0.0 0.026936188683712524 0.0 34-35 0.0 5.7189360262659295E-5 0.0 0.05278577952243453 0.0 36-37 0.0 5.7189360262659295E-5 0.0 0.09639266672271224 0.0 38-39 0.0 5.7189360262659295E-5 0.0 0.16078788637846658 0.0 40-41 0.0 5.7189360262659295E-5 0.0 0.23053031121877962 0.0 42-43 0.0 5.7189360262659295E-5 0.0 0.29847127121081884 0.0 44-45 0.0 5.7189360262659295E-5 0.0 0.38028065106655295 0.0 46-47 0.0 5.7189360262659295E-5 0.0 0.4765875337488712 0.0 48-49 0.0 5.7189360262659295E-5 0.0 0.5813012523898005 0.0 50-51 0.0 5.7189360262659295E-5 0.0 0.7017706397830922 0.0 52-53 0.0 5.7189360262659295E-5 0.0 0.8144336795005309 0.0 54-55 0.0 5.7189360262659295E-5 0.0 0.9345599307322467 0.0 56-57 0.0 5.7189360262659295E-5 0.0 1.0787057132742794 0.0 58-59 0.0 5.7189360262659295E-5 0.0 1.2323449296199138 0.0 60-61 0.0 5.7189360262659295E-5 0.0 1.4047708508118317 0.0 62-63 0.0 5.7189360262659295E-5 0.0 1.57570984863692 0.0 64-65 0.0 5.7189360262659295E-5 0.0 1.751252589963153 0.0 66-67 0.0 5.7189360262659295E-5 0.0 1.9556759582220287 0.0 68-69 0.0 5.7189360262659295E-5 0.0 2.190638444861164 0.0 70-71 0.0 5.7189360262659295E-5 0.0 2.450792844696001 0.0 72-73 0.0 5.7189360262659295E-5 0.0 2.7076588563157356 0.0 74-75 0.0 5.7189360262659295E-5 0.0 2.9638100009321864 0.0 76-77 0.0 5.7189360262659295E-5 0.0 3.2425223481722565 0.0 78-79 0.0 5.7189360262659295E-5 0.0 3.5613530316365822 0.0 80-81 0.0 5.7189360262659295E-5 0.0 3.9017726986000616 0.0 82-83 0.0 5.7189360262659295E-5 0.0 4.262608967177311 0.0 84-85 0.0 8.578404039398894E-5 0.0 4.616124997640939 0.0 86-87 0.0 1.1437872052531859E-4 0.0 4.982851770325242 0.0 88-89 0.0 1.1437872052531859E-4 0.0 5.38755227822395 0.0 90-91 0.0 1.1437872052531859E-4 0.0 5.825965913997496 0.0 92-93 0.0 1.1437872052531859E-4 0.0 6.279305972799596 0.0 94-95 0.0 1.1437872052531859E-4 0.0 6.7401664324762365 0.0 96-97 0.0 1.1437872052531859E-4 0.0 7.207489289862556 8.578404039398894E-5 98-99 0.0 1.1437872052531859E-4 0.0 7.708067760241613 1.1437872052531859E-4 100-101 0.0 1.1437872052531859E-4 0.0 8.255598695396314 1.1437872052531859E-4 102-103 0.0 1.1437872052531859E-4 0.0 8.837843572230447 1.1437872052531859E-4 104-105 0.0 1.1437872052531859E-4 0.0 9.422776348996928 1.1437872052531859E-4 106-107 0.0 1.1437872052531859E-4 0.0 9.998444449400855 1.1437872052531859E-4 108-109 0.0 1.1437872052531859E-4 0.0 10.594614935458948 1.1437872052531859E-4 110-111 0.0 1.1437872052531859E-4 0.0 11.230875163061164 1.1437872052531859E-4 112-113 0.0 1.1437872052531859E-4 0.0 11.905280693958574 1.1437872052531859E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2140 0.0 48.673172 1 GGTATCA 1375 0.0 43.72039 1 ATCAACG 2275 0.0 43.39917 3 CAACGCA 2275 0.0 43.14143 5 TCAACGC 2280 0.0 43.04313 4 AACGCAG 2400 0.0 40.89448 6 GTACATG 6105 0.0 40.362682 1 TATCAAC 2655 0.0 37.85968 2 TACATGG 6530 0.0 36.98006 2 ACATGGG 6485 0.0 36.04436 3 ACGCAGA 3005 0.0 32.859097 7 CGCAGAG 3050 0.0 32.37429 8 CATGGGG 3525 0.0 29.359243 4 CATGGGA 3760 0.0 26.416992 4 GCAGAGT 3945 0.0 23.67253 9 GAGTACA 4485 0.0 23.62234 1 TAAGGTA 610 0.0 22.426046 4 GTGTAGG 1075 0.0 22.1471 1 AGGACCG 525 0.0 21.527138 5 ATGGGGA 2350 0.0 20.75573 5 >>END_MODULE