FastQCFastQC Report
Thu 26 May 2016
SRR1364436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748577
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.11397839500347996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.10294084847278673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.065.036171
GTATCAA38350.053.1255461
ATCAACG47050.041.891373
TCAACGC47550.041.450874
CAACGCA48200.040.9814155
AACGCAG50100.039.42616
TATCAAC50350.039.0274932
GTACATG59850.034.8374671
TACATGG63400.032.5909042
ACATGGG62800.032.4281853
ACGCAGA61250.032.3460777
CGCAGAG61550.032.090848
TATTACG850.007183935528.021882
GCAGAGT70350.027.6537939
CATGGGG34200.026.8133344
CATGGGA36700.024.6623084
CAGAGTA68500.024.31859610-11
AGAGTAC67750.023.57794810-11
GAGTACT42550.023.14032612-13
GTATAGG4700.022.8148351
Produced by FastQC (version 0.11.5)