##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364436_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1748577 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.915366037640894 33.0 31.0 34.0 30.0 34.0 2 31.845247878703656 33.0 31.0 34.0 30.0 34.0 3 32.063776430777715 33.0 31.0 34.0 30.0 34.0 4 35.762746507588744 37.0 35.0 37.0 35.0 37.0 5 35.705914580827724 37.0 35.0 37.0 33.0 37.0 6 35.76963668171319 37.0 35.0 37.0 33.0 37.0 7 35.67199156800072 37.0 35.0 37.0 33.0 37.0 8 35.68004440181931 37.0 35.0 37.0 33.0 37.0 9 37.313971875416414 39.0 37.0 39.0 34.0 39.0 10-11 37.27021229262423 39.0 37.0 39.0 33.0 39.0 12-13 37.25311124417169 39.0 37.0 39.0 33.0 39.0 14-15 38.55942832371694 40.0 38.0 41.0 34.0 41.0 16-17 38.57315062476516 40.0 38.0 41.0 34.0 41.0 18-19 38.61334673851938 40.0 38.0 41.0 34.0 41.0 20-21 38.616874178260375 40.0 38.0 41.0 34.0 41.0 22-23 38.59781010501682 40.0 38.0 41.0 34.0 41.0 24-25 38.54954971957197 40.0 38.0 41.0 34.0 41.0 26-27 38.44335565434064 40.0 38.0 41.0 34.0 41.0 28-29 38.36299602476757 40.0 38.0 41.0 34.0 41.0 30-31 38.245111024564544 40.0 38.0 41.0 33.0 41.0 32-33 38.1332732273157 40.0 37.5 41.0 33.0 41.0 34-35 38.04205105065434 40.0 37.0 41.0 33.0 41.0 36-37 37.949452326091446 40.0 37.0 41.0 33.0 41.0 38-39 37.858229005642876 40.0 37.0 41.0 33.0 41.0 40-41 37.73920307770261 40.0 37.0 41.0 33.0 41.0 42-43 37.612184650718845 40.0 37.0 41.0 32.0 41.0 44-45 37.4623551036071 40.0 37.0 41.0 32.0 41.0 46-47 37.282056495081434 40.0 36.5 41.0 31.0 41.0 48-49 37.077810413839366 40.0 36.0 41.0 31.0 41.0 50-51 36.86580116288845 39.5 36.0 41.0 30.5 41.0 52-53 36.73898461434641 39.0 35.5 41.0 30.0 41.0 54-55 36.59193189662223 39.0 35.0 41.0 30.0 41.0 56-57 36.38991763016441 39.0 35.0 41.0 30.0 41.0 58-59 36.17828497115083 39.0 35.0 40.5 29.5 41.0 60-61 35.93873361024421 38.0 35.0 40.0 29.0 41.0 62-63 35.99160260028583 38.0 35.0 40.0 30.0 41.0 64-65 35.78512298857871 38.0 35.0 40.0 29.5 41.0 66-67 35.519870443223255 37.0 35.0 40.0 29.0 41.0 68-69 35.20507790048708 37.0 34.0 39.5 29.0 41.0 70-71 34.86845789461945 36.0 34.0 39.0 29.0 41.0 72-73 34.521545805532156 36.0 34.0 39.0 29.0 40.5 74-75 34.18065747176132 35.0 34.0 37.5 29.0 40.0 76-77 33.85620621797038 35.0 34.0 37.0 29.0 39.0 78-79 33.52546956753977 35.0 33.5 37.0 28.0 39.0 80-81 33.23256739623133 35.0 33.0 36.0 28.0 38.5 82-83 32.95978901701211 35.0 33.0 36.0 27.5 37.0 84-85 32.7347809104203 35.0 33.0 35.5 27.0 37.0 86-87 32.54218944890617 35.0 33.0 35.0 27.0 36.5 88-89 32.362593697618124 35.0 33.0 35.0 27.0 36.0 90-91 32.23577057229964 35.0 33.0 35.0 26.5 36.0 92-93 32.114842526237055 35.0 33.0 35.0 26.0 36.0 94-95 32.00506726326607 35.0 33.0 35.0 26.0 35.0 96-97 31.95335692966338 35.0 33.0 35.0 26.0 35.0 98-99 31.879153448775774 35.0 33.0 35.0 25.5 35.0 100-101 31.86990936058292 35.0 33.0 35.0 25.5 35.0 102-103 30.740895882766388 33.5 30.5 34.5 24.0 35.0 104-105 31.708433200253694 35.0 32.5 35.0 25.0 35.0 106-107 31.90147388419269 35.0 33.0 35.0 26.5 35.0 108-109 31.91104766904746 35.0 33.0 35.0 26.0 35.0 110-111 31.85482967006886 35.0 33.0 35.0 25.0 35.0 112-113 31.80369551927081 35.0 33.0 35.0 25.0 35.0 114-115 31.699936862946267 35.0 33.0 35.0 25.0 35.0 116-117 31.61717670997617 35.0 33.0 35.0 25.0 35.0 118-119 31.522357608501082 35.0 33.0 35.0 25.0 35.0 120-121 31.405675586491185 35.0 33.0 35.0 25.0 35.0 122-123 31.22541586673049 35.0 33.0 35.0 24.5 35.0 124-125 30.205992644304484 34.5 31.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2866439439683539 1101 2 0.5332422846920544 1101 3 0.35705788682243167 1101 4 0.25148478133426266 1101 5 0.2901387126729347 1101 6 0.20495651898102807 1101 7 0.30602547789827383 1101 8 0.3118614421985342 1101 9 0.38792556244948884 1101 10-11 0.362401312098541 1101 12-13 0.40310255327693056 1101 14-15 0.43109765775109565 1101 16-17 0.46174017998027495 1101 18-19 0.24053658415949286 1101 20-21 0.32940270249418546 1101 22-23 0.4345062343113071 1101 24-25 0.37840654379357375 1101 26-27 0.35289590898707957 1101 28-29 0.383360913508362 1101 30-31 0.3941644861003297 1101 32-33 0.3772696166456271 1101 34-35 0.3376493746554061 1101 36-37 0.31461242950882706 1101 38-39 0.35604296263776547 1101 40-41 0.41869390310000654 1101 42-43 0.2861271520406774 1101 44-45 0.3855393649432983 1101 46-47 0.3274782727316463 1101 48-49 0.23461648020017378 1101 50-51 0.224113182585576 1101 52-53 0.23307539654938836 1101 54-55 0.2645919157957337 1101 56-57 0.34679441466168726 1101 58-59 0.29817294134929995 1101 60-61 0.05566488753970589 1101 62-63 0.2906981404020357 1101 64-65 0.4161589542782238 1101 66-67 0.44508539454676566 1101 68-69 0.490769833487974 1101 70-71 0.46657438073582114 1101 72-73 0.4232681697678373 1101 74-75 0.402282930977897 1101 76-77 0.476004136722473 1101 78-79 0.37123257355157335 1101 80-81 0.260745099419907 1101 82-83 0.28266795121277255 1101 84-85 0.1334757682649368 1101 86-87 0.3704470103660569 1101 88-89 0.3824874692060547 1101 90-91 0.2919460934075033 1101 92-93 0.3197913335225877 1101 94-95 0.32414713555052543 1101 96-97 0.2280181329810027 1101 98-99 0.24435895343685132 1101 100-101 0.3762320476452956 1101 102-103 0.4843156362509049 1101 104-105 0.3949603381767517 1101 106-107 0.35765412127101825 1101 108-109 0.37686159204939784 1101 110-111 0.3559363794930448 1101 112-113 0.34331539131052224 1101 114-115 0.3432595875752682 1101 116-117 0.35648011441286087 1101 118-119 0.38309143657652456 1101 120-121 0.42342599551165705 1101 122-123 0.39202146375990665 1101 124-125 0.31208520567444253 1102 1 0.32212618188319553 1102 2 0.39902146953488327 1102 3 0.24402982429015196 1102 4 0.23172937827870044 1102 5 0.13431950713762575 1102 6 0.21571931451529025 1102 7 0.29425659590493325 1102 8 0.31259161927411583 1102 9 0.32222535029774235 1102 10-11 0.3086755671905266 1102 12-13 0.33088228935613273 1102 14-15 0.28230234800678033 1102 16-17 0.4087842149722789 1102 18-19 0.3347853367797242 1102 20-21 0.2948758099196169 1102 22-23 0.3985791198153237 1102 24-25 0.34447158240559617 1102 26-27 0.2931345189162897 1102 28-29 0.3431784609665982 1102 30-31 0.3820952341554573 1102 32-33 0.30834155590421375 1102 34-35 0.3151079420203118 1102 36-37 0.3369808909842362 1102 38-39 0.28886033814403334 1102 40-41 0.19554880402960606 1102 42-43 0.2863108974981827 1102 44-45 0.26174413891818915 1102 46-47 0.26946668686711206 1102 48-49 0.23378911604623198 1102 50-51 0.3087415064382526 1102 52-53 0.16833999364815355 1102 54-55 0.14647649190791867 1102 56-57 0.12739272889174913 1102 58-59 0.08652802202040277 1102 60-61 0.15830747335953532 1102 62-63 0.15306602239493827 1102 64-65 0.33732348989069294 1102 66-67 0.24257715257292745 1102 68-69 0.17675641773999473 1102 70-71 0.19943687725419323 1102 72-73 0.22475304272649055 1102 74-75 0.192168762732571 1102 76-77 0.23575644143941332 1102 78-79 0.3016088426073438 1102 80-81 0.15449661819550897 1102 82-83 0.06165113995939464 1102 84-85 0.09527185536028071 1102 86-87 0.18167723570758199 1102 88-89 0.10115388194872565 1102 90-91 0.14673978962792233 1102 92-93 0.08423249617415252 1102 94-95 0.1193892493105082 1102 96-97 0.058823655886069304 1102 98-99 0.11177786992978156 1102 100-101 0.11526770722232982 1102 102-103 0.060060260349484196 1102 104-105 0.06261387228475712 1102 106-107 0.05843985877970681 1102 108-109 0.06341127896140719 1102 110-111 0.07349151077422178 1102 112-113 0.027101179633138628 1102 114-115 0.13418550306662524 1102 116-117 0.11457718122675686 1102 118-119 -0.05525235292164865 1102 120-121 -0.08383384284184459 1102 122-123 -0.08512741534184087 1102 124-125 -0.19225093960502448 1103 1 0.32698773026335815 1103 2 0.44051290025535295 1103 3 0.32146516518092483 1103 4 0.21861137831452027 1103 5 0.16075737314525185 1103 6 0.14548768429268222 1103 7 0.2412418929195681 1103 8 0.3097739470378684 1103 9 0.3779743429372928 1103 10-11 0.3401357294427143 1103 12-13 0.33868443481002686 1103 14-15 0.45699157954746283 1103 16-17 0.4899105245408677 1103 18-19 0.3313612066914402 1103 20-21 0.41877165332655153 1103 22-23 0.3453169195610215 1103 24-25 0.37238254069639254 1103 26-27 0.414760407632059 1103 28-29 0.41941356546345077 1103 30-31 0.48556363437752026 1103 32-33 0.44815308548031396 1103 34-35 0.41879532897208804 1103 36-37 0.35964533698076195 1103 38-39 0.3595025058046275 1103 40-41 0.3200102019772757 1103 42-43 0.36143803086375215 1103 44-45 0.3150972433792276 1103 46-47 0.2829037469863849 1103 48-49 0.29802801717268324 1103 50-51 0.2333117888573497 1103 52-53 0.2764311843929761 1103 54-55 0.2855799142471511 1103 56-57 0.16046688852928526 1103 58-59 0.1578818070093888 1103 60-61 0.11613565796169922 1103 62-63 0.3934375906536829 1103 64-65 0.41716553577261095 1103 66-67 0.3241996028317047 1103 68-69 0.4721914362754305 1103 70-71 0.4202917636239363 1103 72-73 0.43669286972912147 1103 74-75 0.38574092555784034 1103 76-77 0.31524532913439174 1103 78-79 0.3247842576398412 1103 80-81 0.3144562879603683 1103 82-83 0.3372107309262873 1103 84-85 0.23909713876435745 1103 86-87 0.3227706650311788 1103 88-89 0.34810264535781954 1103 90-91 0.23046564431729877 1103 92-93 0.20087534436269294 1103 94-95 0.24744562567828154 1103 96-97 0.2810742692566528 1103 98-99 0.29797103241477885 1103 100-101 0.23083367366155727 1103 102-103 0.2800399877801354 1103 104-105 0.37922204936528914 1103 106-107 0.35265837988502824 1103 108-109 0.2895708448562182 1103 110-111 0.2630397476695805 1103 112-113 0.2702468663395585 1103 114-115 0.2409088326391462 1103 116-117 0.2585691581604408 1103 118-119 0.20931520738566434 1103 120-121 0.17562266604979015 1103 122-123 0.144362152881083 1103 124-125 -0.016131596493579536 1104 1 0.2975011802502969 1104 2 0.38487435233161094 1104 3 0.24221940910884854 1104 4 0.13994575630703565 1104 5 0.127562283043396 1104 6 0.2244214354896883 1104 7 0.20014360790171537 1104 8 0.1779978523221004 1104 9 0.23092602745022361 1104 10-11 0.19102525673444148 1104 12-13 0.18479638745214544 1104 14-15 0.23592637540364336 1104 16-17 0.24725395624627566 1104 18-19 0.24792776018669116 1104 20-21 0.1544067190530285 1104 22-23 0.15806733307194776 1104 24-25 0.24386682728123077 1104 26-27 0.10141105619130286 1104 28-29 0.19992753777998473 1104 30-31 0.07819217098412423 1104 32-33 -4.486382073309869E-4 1104 34-35 -0.010933930190439867 1104 36-37 -0.07425211230974327 1104 38-39 -0.22925034524438814 1104 40-41 -0.13749828587856427 1104 42-43 -0.3548318394243992 1104 44-45 -0.17692608734245852 1104 46-47 -0.06082359696764428 1104 48-49 -0.1099538754015299 1104 50-51 -0.04058391933452299 1104 52-53 -0.09809932836056845 1104 54-55 -0.10364860494846084 1104 56-57 -0.07433105697433007 1104 58-59 -0.29964339539332485 1104 60-61 -0.23908711560842733 1104 62-63 0.09492936396264184 1104 64-65 0.07292421950204186 1104 66-67 0.008544688580812476 1104 68-69 0.044545575737430454 1104 70-71 -0.04481035181090931 1104 72-73 -0.010444241788249542 1104 74-75 -0.06879098007461693 1104 76-77 -6.456780911676674E-4 1104 78-79 -0.01734475776888189 1104 80-81 0.02965573080880546 1104 82-83 0.02458114966050573 1104 84-85 0.036704869242285554 1104 86-87 0.13465993115810448 1104 88-89 0.13344468418431177 1104 90-91 0.0414133243033703 1104 92-93 0.19997345527067267 1104 94-95 0.14228152713209852 1104 96-97 0.156253515360941 1104 98-99 0.0924151866761207 1104 100-101 0.037728076029452495 1104 102-103 0.0542644153939591 1104 104-105 0.08895722826317609 1104 106-107 0.13746346389303454 1104 108-109 -0.04760962001711988 1104 110-111 0.09230134773922671 1104 112-113 -0.041008988104593413 1104 114-115 0.005103835686071534 1104 116-117 -0.10730043355193075 1104 118-119 -0.3134761219431432 1104 120-121 -0.1714945969431554 1104 122-123 -0.1712245103033858 1104 124-125 -0.3442437232455582 1105 1 0.26603030358407764 1105 2 0.4140487265761159 1105 3 0.25364342540101603 1105 4 0.17968518866821626 1105 5 0.15160495567774035 1105 6 0.09972466747912989 1105 7 0.159082985717788 1105 8 0.15206470705047792 1105 9 0.20587376035501137 1105 10-11 0.26477408028671334 1105 12-13 0.2680305816340436 1105 14-15 0.17515865285987786 1105 16-17 0.18548568761411133 1105 18-19 0.3047333788436646 1105 20-21 0.3020971857005321 1105 22-23 0.25547522924250643 1105 24-25 0.36505760584270774 1105 26-27 0.26543198724334616 1105 28-29 0.2756041744362179 1105 30-31 0.209090132742638 1105 32-33 0.3004365092715844 1105 34-35 0.23939318121859543 1105 36-37 0.1992532972788581 1105 38-39 0.29095683699684116 1105 40-41 0.23219463657369488 1105 42-43 0.22005669673372097 1105 44-45 0.3629794654907954 1105 46-47 0.22559836432443348 1105 48-49 0.21320709068427846 1105 50-51 0.19544759194121752 1105 52-53 0.289336560415812 1105 54-55 0.25897970511504553 1105 56-57 0.07686138470349135 1105 58-59 0.23209243291189097 1105 60-61 0.2639623770773696 1105 62-63 0.4632291556589152 1105 64-65 0.5284238139183088 1105 66-67 0.4485376665340084 1105 68-69 0.4704480712403267 1105 70-71 0.4934903893272633 1105 72-73 0.5110504856231586 1105 74-75 0.5264351113534502 1105 76-77 0.44366604981026114 1105 78-79 0.3842114348544001 1105 80-81 0.3879478313884732 1105 82-83 0.3348430235784505 1105 84-85 0.31304923755438097 1105 86-87 0.3186202890913208 1105 88-89 0.3952560918880508 1105 90-91 0.19259633528312037 1105 92-93 0.2226762041279997 1105 94-95 0.10450317020184485 1105 96-97 0.29092286425408176 1105 98-99 0.3487944485710628 1105 100-101 0.28935235446953556 1105 102-103 0.25576459731722423 1105 104-105 0.18799822822303014 1105 106-107 0.19568833888849468 1105 108-109 0.21769273899554165 1105 110-111 0.24691260862993403 1105 112-113 0.3508657645785398 1105 114-115 0.3147543072528336 1105 116-117 0.31681430745752337 1105 118-119 0.18446155006354914 1105 120-121 0.25342857154199905 1105 122-123 0.21660587476858595 1105 124-125 0.13610913779246658 1106 1 0.25391681556654433 1106 2 0.42198258165308644 1106 3 0.20750421965163923 1106 4 0.1754513920178411 1106 5 0.17458265577629106 1106 6 0.11150138761573913 1106 7 0.17704113923679898 1106 8 0.14475373463636032 1106 9 0.19238749812742384 1106 10-11 0.23144816520838418 1106 12-13 0.251087997674027 1106 14-15 0.2698428984922998 1106 16-17 0.3080410486929779 1106 18-19 0.30423719161777996 1106 20-21 0.30626051937656484 1106 22-23 0.2570736855767777 1106 24-25 0.1571650004428875 1106 26-27 0.26412560119150186 1106 28-29 0.24334059526818663 1106 30-31 0.27126524451798417 1106 32-33 0.24272965279788394 1106 34-35 0.18836538721121343 1106 36-37 0.16097798514793737 1106 38-39 0.2043044927864699 1106 40-41 0.2488385678559979 1106 42-43 0.17641610554686338 1106 44-45 0.030054763077750124 1106 46-47 0.016578978229965458 1106 48-49 0.012371627998305712 1106 50-51 0.014193940279071171 1106 52-53 -0.10122691769678482 1106 54-55 -0.03003879078882221 1106 56-57 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-0.7095874189068994 2216 84-85 -0.6405013598707683 2216 86-87 -0.5772689164064957 2216 88-89 -0.5288005241902596 2216 90-91 -0.6186836580788224 2216 92-93 -0.5953401545757373 2216 94-95 -0.6395296548798015 2216 96-97 -0.5628223792116529 2216 98-99 -0.5777326375882481 2216 100-101 -0.5842801419283745 2216 102-103 -0.6114496998146493 2216 104-105 -0.6732577049568214 2216 106-107 -0.7624542983072686 2216 108-109 -0.5861084090109365 2216 110-111 -0.6699525938530151 2216 112-113 -0.5655208012370245 2216 114-115 -0.6007413796750782 2216 116-117 -0.6091500562769276 2216 118-119 -0.6971183685924984 2216 120-121 -0.5952287898191813 2216 122-123 -0.6515171313166697 2216 124-125 -0.5864171591262739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56.0 3 8.0 4 37.0 5 82.0 6 277.0 7 638.0 8 1085.0 9 1894.0 10 2798.0 11 3775.0 12 5059.0 13 6568.0 14 8890.0 15 7069.0 16 4345.0 17 3999.0 18 4210.0 19 4085.0 20 4322.0 21 4472.0 22 4992.0 23 5935.0 24 7708.0 25 10660.0 26 14298.0 27 19303.0 28 25187.0 29 32227.0 30 40382.0 31 50626.0 32 63218.0 33 80781.0 34 107184.0 35 153740.0 36 284116.0 37 543490.0 38 240225.0 39 836.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8097958389711 19.11149760996862 12.81537400915964 25.263332541900642 2 15.838646968897145 20.47933958978295 38.56907306823206 25.11294037308785 3 18.926262392304547 24.406356069119536 29.73684135276641 26.930540185809505 4 12.931799268071837 16.61032781086628 36.72908012175146 33.728792799310426 5 13.091435470486063 38.35482153345851 34.86413048140699 13.689612514648447 6 32.935695711427066 36.89182689695678 16.724513704572345 13.447963687043806 7 28.610834046330314 32.44649091100425 21.047327748317404 17.895347294348028 8 26.481876405785965 35.52002571233637 19.512609396097513 18.485488485780152 9 26.236019346016793 15.301756799957907 19.66438995823461 38.79783389579069 10-11 24.94165255519202 26.321317276848543 28.04700622277429 20.690023945185143 12-13 25.62649514433737 23.58357681703465 28.2133986664585 22.576529372169485 14-15 22.354863411791417 25.29825681110983 25.626409360296975 26.720470416801778 16-17 22.417428571918766 28.700394663775175 26.412334143706566 22.469842620599493 18-19 22.443748513075786 27.07826254049009 28.382438052449537 22.09555089398459 20-21 22.843174764394135 26.45411097137844 28.459198536867408 22.24351572736002 22-23 22.677611566433733 26.475328224035888 28.30001195257629 22.547048256954085 24-25 22.692795341583473 26.639147146508275 28.292920471903727 22.37513704000453 26-27 22.33455871840125 27.016480933519237 28.126218855908213 22.5227414921713 28-29 22.294398784833525 26.9619637108558 28.362110230834737 22.38152727347594 30-31 22.38437437359295 26.583192431959148 28.352101596539104 22.680331597908797 32-33 22.226244540560696 26.76956748258727 28.27599242126598 22.728195555586055 34-35 22.8407728112631 26.680466459298046 27.974032599079134 22.504728130359716 36-37 22.94522917778285 26.327350754356253 27.957390495242702 22.770029572618192 38-39 22.584535882606257 26.528828870561604 28.07991869960545 22.80671654722669 40-41 22.741631316099127 26.38886625118133 28.17991401483794 22.689588417881595 42-43 22.739932458902015 26.32292765782227 28.027839971633885 22.90929991164183 44-45 22.655307715931297 26.551475857225622 27.99547860917763 22.79773781766545 46-47 22.957153159397613 26.887406159408478 27.309892558348874 22.845548122845035 48-49 22.924069114485665 26.822153099348782 27.341975789456225 22.911801996709325 50-51 22.969877792056053 26.844742896652534 27.34975354245195 22.835625768839463 52-53 23.168668008329057 26.717039055186014 27.19259718045016 22.921695756034765 54-55 22.69668421808133 26.653415891893808 27.45844192163113 23.191457968393728 56-57 22.724262466651226 27.067309479487466 27.3823678273327 22.82606022652861 58-59 22.781349429547657 26.58935008837229 27.360609550040504 23.26869093203955 60-61 22.632899722859882 26.683886412585338 27.724906123504066 22.958307741050707 62-63 22.559167684880517 26.80641336041453 27.690279219830394 22.944139734874554 64-65 22.431239802422198 26.70482912676994 27.481517828497115 23.382413242310747 66-67 22.306166671527762 26.992262851450064 27.351840954101537 23.349729522920637 68-69 22.48568407339225 26.619273843817 27.733322581733606 23.161719501057146 70-71 22.465296066458613 26.62407775007906 27.474483537184806 23.436142646277517 72-73 22.535724763622074 26.69616493869015 27.264284043539405 23.503826254148375 74-75 22.44845379986126 26.922120101087916 27.201919046172975 23.427507052877854 76-77 22.624825786911302 26.705915724614933 27.290390986499308 23.37886750197446 78-79 22.7218475365969 26.745919682118657 27.198373305836686 23.33385947544775 80-81 22.635062682398317 27.09091449790315 27.03278151319616 23.24124130650237 82-83 22.66781007293647 26.855902501263166 27.141277490575906 23.335009935224452 84-85 22.686647485355234 26.750351857539016 27.086425133122532 23.476575523983218 86-87 22.774833478880254 26.61089560253852 27.213499891626164 23.400771026955063 88-89 22.608555413916573 26.771797867637513 27.00069828208881 23.61894843635711 90-91 22.565108072380145 26.94539291599695 27.04524573888372 23.44425327273918 92-93 22.60746334296595 26.48977194546639 27.252908514543 23.64985619702466 94-95 22.438653354817585 26.331640042491927 27.257083298185513 23.97262330450497 96-97 22.67878359810525 26.249408427424946 27.187031299372183 23.88477667509762 98-99 22.5240410119202 26.334055605108276 26.995081555131108 24.146821827840416 100-101 22.30840620330185 26.23316006606559 27.254087987358876 24.204345743273677 102-103 22.33283688283997 26.012404398963174 27.271861552082687 24.382897166114166 104-105 22.261741765236756 26.058420293485508 27.26794135780228 24.411896583475453 106-107 22.431715945195453 26.174262680915927 27.02326991326331 24.370751460625318 108-109 22.39194184658485 26.07234515463517 26.868900288902452 24.666812709877536 110-111 22.31085620619159 26.218428134838167 27.058771718860946 24.41194394010929 112-113 22.30206219911667 26.246305756799764 27.164780046868813 24.28685199721475 114-115 22.23037199269895 26.341688134877845 26.873911040536957 24.55402883188625 116-117 22.0768373368745 26.303760143247914 27.06841048463979 24.550992035237794 118-119 21.92654692422358 26.509922316010588 26.7387679622958 24.82476279747003 120-121 21.940341655641014 26.556046972003177 26.857321124905532 24.646290247450278 122-123 21.965266514618786 26.506972546740997 26.81705414475355 24.71070679388667 124-125 21.883945005956782 26.543707621391782 26.735268584373923 24.837078788277513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 34.0 1 32.5 2 77.0 3 114.0 4 74.5 5 59.5 6 142.5 7 347.0 8 590.0 9 714.5 10 598.5 11 418.5 12 385.5 13 418.5 14 492.5 15 585.0 16 676.0 17 791.0 18 934.5 19 1054.5 20 1247.5 21 1545.5 22 1893.5 23 2409.0 24 3325.5 25 4605.5 26 6291.5 27 8541.5 28 11087.0 29 14056.0 30 17569.0 31 21502.5 32 26426.5 33 29780.5 34 32091.5 35 35579.5 36 40451.5 37 45116.0 38 48288.0 39 50978.5 40 53231.5 41 56752.5 42 60011.5 43 62831.0 44 66055.0 45 69174.0 46 69792.5 47 69309.5 48 69311.5 49 67762.5 50 66895.5 51 65576.5 52 63908.5 53 63551.0 54 63132.5 55 58867.0 56 52046.0 57 46588.5 58 42254.0 59 36975.0 60 29922.0 61 24075.5 62 19610.5 63 14848.5 64 11186.5 65 8621.5 66 6351.5 67 5377.5 68 4101.5 69 2543.0 70 1786.5 71 1309.0 72 981.5 73 737.5 74 637.5 75 565.0 76 344.0 77 140.0 78 48.0 79 26.0 80 16.5 81 5.0 82 2.5 83 1.5 84 0.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039575037301760235 2 0.005032663703114018 3 6.862723231519115E-4 4 0.08069418733061226 5 0.005604557305740611 6 0.0 7 0.005089853063376677 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.718936026265929E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.290829628892523E-4 28-29 5.718936026265929E-4 30-31 7.720563635459005E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0011151925251218562 42-43 6.862723231519115E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.862723231519115E-4 58-59 4.8610956223260404E-4 60-61 1.7156808078797788E-4 62-63 4.8610956223260404E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.8594680131329644E-5 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.0016276091930753E-4 92-93 2.2875744105063715E-4 94-95 5.432989224952632E-4 96-97 0.003402766935628228 98-99 0.009064513601631498 100-101 0.017986053802606348 102-103 0.009436244443338784 104-105 0.004289202019699447 106-107 0.010408463567803991 108-109 0.013496689021987593 110-111 0.007177264712963741 112-113 0.004546554140881414 114-115 0.0035171456561535466 116-117 0.0 118-119 0.001058003164859197 120-121 2.0016276091930753E-4 122-123 2.8594680131329645E-4 124-125 0.03256934066958447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1748577.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.0074507194055 #Duplication Level Percentage of deduplicated Percentage of total 1 84.41946786583233 60.78830672107357 2 10.06307234568499 14.492323720354483 3 2.721993924730438 5.880115301806444 4 1.0765379249235338 3.100750063060096 5 0.500529773253858 1.8020936490586188 6 0.2899070164307817 1.2525279119309636 7 0.18863637777436942 0.9508257273532547 8 0.11520124953367678 0.6636278638888132 9 0.08986359029402849 0.5823763244609491 >10 0.4827621138581796 6.368962657813013 >50 0.03582047645518308 1.777100448372778 >100 0.015589918812034342 1.7297893125748782 >500 1.5435560415945696E-4 0.06980065235539427 >1k 4.630668124783708E-4 0.5413996458966966 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1993 0.11397839500347996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1800 0.10294084847278673 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.7189360262659295E-5 0.0 4 0.0 0.0 0.0 5.7189360262659295E-5 0.0 5 0.0 0.0 0.0 5.7189360262659295E-5 0.0 6 0.0 0.0 0.0 5.7189360262659295E-5 0.0 7 0.0 0.0 0.0 1.1437872052531859E-4 0.0 8 0.0 0.0 0.0 1.1437872052531859E-4 0.0 9 0.0 0.0 0.0 1.7156808078797788E-4 0.0 10-11 0.0 5.7189360262659295E-5 0.0 3.1454148144462613E-4 0.0 12-13 0.0 5.7189360262659295E-5 0.0 4.5751488210127436E-4 0.0 14-15 0.0 5.7189360262659295E-5 0.0 4.5751488210127436E-4 0.0 16-17 0.0 5.7189360262659295E-5 0.0 5.147042423639337E-4 0.0 18-19 0.0 5.7189360262659295E-5 0.0 5.718936026265929E-4 0.0 20-21 0.0 5.7189360262659295E-5 0.0 5.718936026265929E-4 0.0 22-23 0.0 5.7189360262659295E-5 0.0 7.148670032832412E-4 0.0 24-25 0.0 8.578404039398894E-5 0.0 0.0010865978449905267 0.0 26-27 0.0 1.1437872052531859E-4 0.0 0.001458328686697812 0.0 28-29 0.0 1.1437872052531859E-4 0.0 0.003745903097204184 0.0 30-31 0.0 1.1437872052531859E-4 0.0 0.010865978449905266 0.0 32-33 0.0 1.1437872052531859E-4 0.0 0.02822294928962236 0.0 34-35 0.0 1.1437872052531859E-4 0.0 0.05493038053228425 0.0 36-37 0.0 1.1437872052531859E-4 0.0 0.1000241910993911 0.0 38-39 0.0 1.1437872052531859E-4 0.0 0.16613509156302525 0.0 40-41 0.0 1.1437872052531859E-4 0.0 0.23785054933240002 0.0 42-43 0.0 1.1437872052531859E-4 0.0 0.30704967525021776 0.0 44-45 0.0 1.1437872052531859E-4 0.0 0.39077489867475096 0.0 46-47 0.0 1.1437872052531859E-4 0.0 0.48936935576757556 0.0 48-49 0.0 1.1437872052531859E-4 0.0 0.5956557818157279 0.0 50-51 0.0 1.1437872052531859E-4 0.0 0.7184699329797887 0.0 52-53 0.0 1.1437872052531859E-4 0.0 0.8333919524276026 0.0 54-55 0.0 1.1437872052531859E-4 0.0 0.9560059408307441 0.0 56-57 0.0 1.1437872052531859E-4 0.0 1.102668055224334 0.0 58-59 0.0 1.1437872052531859E-4 0.0 1.2592811183036263 0.0 60-61 0.0 1.1437872052531859E-4 0.0 1.4345093181484143 0.0 62-63 0.0 1.1437872052531859E-4 0.0 1.6091656243905759 0.0 64-65 0.0 1.1437872052531859E-4 0.0 1.7883112954133562 0.0 66-67 0.0 1.1437872052531859E-4 0.0 1.9964233774091733 0.0 68-69 0.0 1.1437872052531859E-4 0.0 2.2348172256640684 0.0 70-71 0.0 1.4297340065664823E-4 0.0 2.4987747179563726 0.0 72-73 0.0 1.7156808078797788E-4 0.0 2.760844961360009 0.0 74-75 0.0 1.7156808078797788E-4 0.0 3.021857201598786 0.0 76-77 0.0 1.7156808078797788E-4 0.0 3.3068031891074856 0.0 78-79 0.0 1.7156808078797788E-4 0.0 3.634040708530422 0.0 80-81 0.0 1.7156808078797788E-4 0.0 3.982667048691593 0.0 82-83 0.0 1.7156808078797788E-4 0.0 4.355141352082294 0.0 84-85 0.0 1.7156808078797788E-4 0.0 4.7197235237567465 0.0 86-87 0.0 1.7156808078797788E-4 0.0 5.0958293515241255 0.0 88-89 0.0 1.7156808078797788E-4 0.0 5.512739787838911 0.0 90-91 0.0 1.7156808078797788E-4 0.0 5.96476449135497 0.0 92-93 0.0 1.7156808078797788E-4 0.0 6.43185859130024 0.0 94-95 0.0 1.7156808078797788E-4 0.0 6.90367081346718 0.0 96-97 0.0 1.7156808078797788E-4 0.0 7.382088406744455 0.0 98-99 0.0 1.7156808078797788E-4 0.0 7.895162752340903 0.0 100-101 0.0 1.7156808078797788E-4 0.0 8.455017994632207 0.0 102-103 0.0 1.7156808078797788E-4 0.0 9.049901720084389 0.0 104-105 0.0 1.7156808078797788E-4 0.0 9.648045239071543 0.0 106-107 0.0 1.7156808078797788E-4 0.0 10.23403601900288 0.0 108-109 0.0 1.7156808078797788E-4 0.0 10.844074924924668 0.0 110-111 0.0 1.7156808078797788E-4 0.0 11.494975628754123 0.0 112-113 0.0 1.7156808078797788E-4 0.0 12.187310024093877 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2235 0.0 65.03617 1 GTATCAA 3835 0.0 53.125546 1 ATCAACG 4705 0.0 41.89137 3 TCAACGC 4755 0.0 41.45087 4 CAACGCA 4820 0.0 40.981415 5 AACGCAG 5010 0.0 39.4261 6 TATCAAC 5035 0.0 39.027493 2 GTACATG 5985 0.0 34.837467 1 TACATGG 6340 0.0 32.590904 2 ACATGGG 6280 0.0 32.428185 3 ACGCAGA 6125 0.0 32.346077 7 CGCAGAG 6155 0.0 32.09084 8 TATTACG 85 0.0071839355 28.02188 2 GCAGAGT 7035 0.0 27.653793 9 CATGGGG 3420 0.0 26.813334 4 CATGGGA 3670 0.0 24.662308 4 CAGAGTA 6850 0.0 24.318596 10-11 AGAGTAC 6775 0.0 23.577948 10-11 GAGTACT 4255 0.0 23.140326 12-13 GTATAGG 470 0.0 22.814835 1 >>END_MODULE