FastQCFastQC Report
Thu 26 May 2016
SRR1364435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957917
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.051.308011
GTATCAA29600.050.071761
TCAACGC35250.041.3399934
ATCAACG35400.040.6586883
CAACGCA36250.040.202665
GTACATG94950.040.1835441
AACGCAG37400.038.966486
TATCAAC38650.038.0091972
TACATGG98550.037.7193872
ACATGGG102250.035.365643
ACGCAGA46650.031.11257
CGCAGAG46250.030.995748
CATGGGA62800.029.928914
CATGGGG49400.027.451834
GCAGAGT54850.024.5091659
AGTACAT71150.022.4863342
GTGTAAG9800.022.472991
GAGTACA75300.022.2915021
ATGGGAG27850.021.9992925
ATGGGGA29000.021.537145
Produced by FastQC (version 0.11.5)