FastQCFastQC Report
Thu 26 May 2016
SRR1364435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957917
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.1099127286805314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.063.6254541
GTATCAA45150.052.1263241
ATCAACG56300.040.6179923
TCAACGC56400.040.545984
CAACGCA57650.039.635435
TATCAAC60000.038.4119572
AACGCAG59750.038.23756
GTACATG101500.035.6320151
ACATGGG104650.034.0865633
TACATGG106000.033.8780332
ACGCAGA75350.030.163127
CGCAGAG75350.030.161588
CATGGGG49150.029.5639154
CATGGGA67400.028.0971874
ACCGTAT1358.254651E-526.4418288
GCAGAGT88500.025.478279
GAGTACA79350.024.5537911
AGTACAT76050.024.1189422
GTGGTAT18200.023.8984221
CGTATAG1501.6714333E-423.8329451
Produced by FastQC (version 0.11.5)