FastQCFastQC Report
Thu 26 May 2016
SRR1364434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684866
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.054.850341
GTATCAA29600.052.0911031
TCAACGC35600.041.7719234
ATCAACG35800.041.7034763
CAACGCA36300.040.9676175
AACGCAG37850.039.289956
GTACATG88250.038.8564641
TATCAAC39900.037.8653872
TACATGG91550.036.449042
ACATGGG92150.035.437133
CATGGGA66250.033.4003874
ACGCAGA46400.032.17837
CGCAGAG46450.032.01568
ATGGGAG28900.027.5812745
GAGTACA64350.025.6263471
CATGGGG33600.025.1387394
GCAGAGT57050.024.920839
GTGGTAT10550.024.82881
ATGGGAT29250.024.6074625
AGAGTAC49450.022.55564310-11
Produced by FastQC (version 0.11.5)