##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364434_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1684866 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.608853760477093 31.0 30.0 34.0 25.0 34.0 2 29.578416325096477 31.0 30.0 34.0 25.0 34.0 3 29.56122207938198 31.0 30.0 34.0 23.0 34.0 4 33.04195051713312 35.0 33.0 37.0 26.0 37.0 5 33.24805592848333 35.0 35.0 37.0 27.0 37.0 6 33.31183666831665 37.0 35.0 37.0 27.0 37.0 7 33.16461071681665 37.0 35.0 37.0 26.0 37.0 8 33.03070629949207 37.0 35.0 37.0 26.0 37.0 9 33.98132195676095 38.0 34.0 39.0 23.0 39.0 10-11 34.15646763600191 38.0 34.0 39.0 25.5 39.0 12-13 34.189112071820546 38.0 34.0 39.0 25.0 39.0 14-15 35.185824570025154 39.0 34.0 41.0 25.0 41.0 16-17 35.30801351561489 39.0 34.5 41.0 25.0 41.0 18-19 35.27379031923013 39.0 34.0 41.0 25.0 41.0 20-21 35.22163097836861 39.0 34.0 41.0 24.5 41.0 22-23 35.12431522744242 39.0 34.0 41.0 24.0 41.0 24-25 35.168766240163905 39.0 34.0 41.0 25.0 41.0 26-27 35.168117820645676 39.0 34.0 41.0 25.0 41.0 28-29 35.10677228930965 39.0 34.0 41.0 25.0 41.0 30-31 34.869918735377176 39.0 34.0 41.0 24.0 41.0 32-33 34.92803166542622 39.0 34.0 41.0 24.5 41.0 34-35 34.8561464235138 39.0 34.0 41.0 24.0 41.0 36-37 34.71553197702369 39.0 33.5 41.0 24.0 41.0 38-39 34.55422983192729 39.0 33.0 41.0 23.5 41.0 40-41 34.50906659639402 39.0 33.0 41.0 23.0 41.0 42-43 34.34663439110291 38.5 33.0 40.5 23.0 41.0 44-45 34.318176044860536 38.0 33.0 40.0 23.0 41.0 46-47 34.15811732208971 38.0 33.0 40.0 22.0 41.0 48-49 33.97913127809571 38.0 33.0 40.0 21.5 41.0 50-51 33.82515137702346 38.0 33.0 40.0 20.5 41.0 52-53 33.67049249020397 38.0 32.5 40.0 20.0 41.0 54-55 33.519984081820155 38.0 32.0 40.0 20.0 41.0 56-57 33.47280050757746 38.0 32.5 40.0 19.5 41.0 58-59 33.448085485730026 37.5 32.0 40.0 19.5 41.0 60-61 33.25624530378084 37.0 32.0 40.0 18.5 41.0 62-63 32.99689085066706 37.0 32.0 40.0 18.0 41.0 64-65 32.69311713809881 36.0 32.0 40.0 15.5 41.0 66-67 32.390429268559046 36.0 31.0 39.0 13.0 41.0 68-69 32.09388550780893 35.0 31.0 39.0 12.5 41.0 70-71 31.7377916107275 35.0 31.0 38.5 8.0 40.0 72-73 31.42126376815723 35.0 31.0 37.5 7.5 40.0 74-75 31.078950492205315 35.0 31.0 37.0 6.5 39.0 76-77 30.74231066446827 35.0 30.0 36.5 2.0 39.0 78-79 30.425607733790105 35.0 30.0 36.0 2.0 39.0 80-81 30.1101413999689 35.0 30.0 36.0 2.0 37.5 82-83 29.825712846006745 34.0 30.0 35.0 2.0 37.0 84-85 29.504513118550676 34.0 29.5 35.0 2.0 36.5 86-87 29.31624384372407 34.0 29.0 35.0 2.0 36.0 88-89 29.162276703310532 34.0 29.0 35.0 2.0 36.0 90-91 29.02597832705984 34.0 29.0 35.0 2.0 36.0 92-93 28.804987458943323 34.0 29.0 35.0 2.0 35.0 94-95 28.71034936902994 34.0 29.0 35.0 2.0 35.0 96-97 28.636215877108327 34.0 29.0 35.0 2.0 35.0 98-99 28.479820056906604 34.0 29.0 35.0 2.0 35.0 100-101 28.347796204564634 34.0 29.0 35.0 2.0 35.0 102-103 27.32779402041468 32.5 26.0 34.5 2.0 35.0 104-105 28.268885181373477 34.0 29.0 35.0 2.0 35.0 106-107 28.522420477355467 34.0 29.0 35.0 2.0 35.0 108-109 28.51527747607228 34.0 29.0 35.0 2.0 35.0 110-111 28.409893427726594 34.0 29.0 35.0 2.0 35.0 112-113 28.291657021982758 34.0 29.0 35.0 2.0 35.0 114-115 28.179103263998442 34.0 29.0 35.0 2.0 35.0 116-117 28.04747439855751 34.0 29.0 35.0 2.0 35.0 118-119 27.78036680661845 34.0 29.0 35.0 2.0 35.0 120-121 27.63281709049859 34.0 28.5 35.0 2.0 35.0 122-123 27.37823393670476 34.0 27.5 35.0 2.0 35.0 124-125 26.18267446788053 33.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0342999242778461 1101 2 0.9596927018121093 1101 3 1.1286722783088443 1101 4 0.9791141386250999 1101 5 1.000340036551627 1101 6 1.0430691861415724 1101 7 1.089635647445533 1101 8 1.1623596532636071 1101 9 1.616726277707322 1101 10-11 1.5131428820383448 1101 12-13 1.3281494626573789 1101 14-15 1.5553125967585757 1101 16-17 1.250151230212417 1101 18-19 1.5186928416575185 1101 20-21 1.4471582509279557 1101 22-23 1.4896698057156925 1101 24-25 1.6009606844241162 1101 26-27 1.456423570265919 1101 28-29 1.4927788904627022 1101 30-31 1.6474076953815384 1101 32-33 1.4752712275033844 1101 34-35 1.4343783081481973 1101 36-37 1.4192008114360277 1101 38-39 1.5418417222192389 1101 40-41 1.5354089328538834 1101 42-43 1.5243489977099571 1101 44-45 1.3666136312663184 1101 46-47 1.2274291961999282 1101 48-49 1.2677026843592074 1101 50-51 1.3882185001868024 1101 52-53 1.2557075053430253 1101 54-55 1.2454961088945495 1101 56-57 1.2218299664316206 1101 58-59 1.2828073305827772 1101 60-61 1.1963680740323426 1101 62-63 1.1601074842706112 1101 64-65 1.2123958578697227 1101 66-67 1.1752742675868149 1101 68-69 1.2034826077193728 1101 70-71 1.0566613716851876 1101 72-73 1.1416450365736353 1101 74-75 1.0137336734956932 1101 76-77 1.2286484750076845 1101 78-79 1.1530799806400935 1101 80-81 1.0866205239507565 1101 82-83 1.0967510469064834 1101 84-85 1.2473528606646092 1101 86-87 1.1715796139615655 1101 88-89 1.0472559204610263 1101 90-91 1.1223302075154606 1101 92-93 1.174195856515567 1101 94-95 1.1182097939161402 1101 96-97 1.1395637534082717 1101 98-99 1.104282883458147 1101 100-101 1.1386747993561208 1101 102-103 1.0146039996234144 1101 104-105 1.1049779035531628 1101 106-107 1.1354156530936024 1101 108-109 1.1156037596331316 1101 110-111 1.1713616525919548 1101 112-113 1.1045196805852413 1101 114-115 1.127926281366829 1101 116-117 1.1068926205788543 1101 118-119 1.1281637360413193 1101 120-121 0.8946980244722731 1101 122-123 0.9166037957341828 1101 124-125 0.6106165379487223 1102 1 0.5611988392181324 1102 2 0.4155811348736975 1102 3 0.62107622565102 1102 4 0.5549560406318363 1102 5 0.43580857187515676 1102 6 0.30445427383913426 1102 7 0.3106388154016102 1102 8 0.4831100677695943 1102 9 0.8187108290102572 1102 10-11 0.6092258382412368 1102 12-13 0.6043122252407969 1102 14-15 0.7187295042507529 1102 16-17 0.5368894866820639 1102 18-19 0.48307796743583253 1102 20-21 0.6556258995139217 1102 22-23 0.7489267534888455 1102 24-25 0.6401268912001967 1102 26-27 0.4665657444593805 1102 28-29 0.4945269028588868 1102 30-31 0.7234896421422334 1102 32-33 0.5416103190900117 1102 34-35 0.4280243378669013 1102 36-37 0.5008372593116306 1102 38-39 0.5966458209172956 1102 40-41 0.5642495129144436 1102 42-43 0.6561781462907845 1102 44-45 0.5108267317711039 1102 46-47 0.6115931332421596 1102 48-49 0.5902761394139446 1102 50-51 0.42441199031210886 1102 52-53 0.3540767423800091 1102 54-55 0.26549235824249706 1102 56-57 0.49809541104860244 1102 58-59 0.6649880461261972 1102 60-61 0.5347880340294182 1102 62-63 0.615191310286292 1102 64-65 0.5568485195633315 1102 66-67 0.5485167350020816 1102 68-69 0.5208196568477845 1102 70-71 0.5547475871170846 1102 72-73 0.4831278527844276 1102 74-75 0.5321273577723957 1102 76-77 0.5370639741164105 1102 78-79 0.45202848337132906 1102 80-81 0.5502003626696919 1102 82-83 0.48911352853856727 1102 84-85 0.6563578407810162 1102 86-87 0.5400004836655441 1102 88-89 0.5691690522161998 1102 90-91 0.5116759227354883 1102 92-93 0.4741609038233463 1102 94-95 0.6043187054775174 1102 96-97 0.6021551547739818 1102 98-99 0.4783461271284324 1102 100-101 0.34996253934798105 1102 102-103 0.38253578853030845 1102 104-105 0.4206965961864668 1102 106-107 0.36177135816481254 1102 108-109 0.4584880080429592 1102 110-111 0.4810192340772126 1102 112-113 0.47621224619445 1102 114-115 0.31462420548215775 1102 116-117 0.31909203195919744 1102 118-119 0.5040996612434654 1102 120-121 0.5305301856638778 1102 122-123 0.4531015541075689 1102 124-125 0.13180825097215632 1103 1 1.0201368369568087 1103 2 0.9695904122276069 1103 3 1.1056771865558517 1103 4 1.0382926530734053 1103 5 0.9175007430673077 1103 6 0.8423446847807625 1103 7 0.9272877307419662 1103 8 1.120233072794825 1103 9 1.557987672137088 1103 10-11 1.3897403839239502 1103 12-13 1.191093115228611 1103 14-15 1.4092453773416977 1103 16-17 1.313452155169898 1103 18-19 1.2789705060142254 1103 20-21 1.1592505753078584 1103 22-23 1.2338171587551017 1103 24-25 1.0785684130343256 1103 26-27 1.3668316675251901 1103 28-29 1.2688935935513257 1103 30-31 1.4710029587899456 1103 32-33 1.2663351735322976 1103 34-35 1.272759903011746 1103 36-37 1.1942016212066306 1103 38-39 1.2608135864005234 1103 40-41 1.3771156931380162 1103 42-43 1.453398014281447 1103 44-45 1.1786957118486256 1103 46-47 1.1331632017944386 1103 48-49 1.1299717940279947 1103 50-51 1.0924289243837393 1103 52-53 0.842835391816223 1103 54-55 0.943525470299079 1103 56-57 0.9114517548933705 1103 58-59 1.1235784268621956 1103 60-61 0.9345648497252483 1103 62-63 1.055492701321839 1103 64-65 1.1042305299971957 1103 66-67 0.9360518356486338 1103 68-69 1.0722006028326732 1103 70-71 1.1283767306959902 1103 72-73 1.0169323056872912 1103 74-75 0.9962174178494436 1103 76-77 0.976236595202213 1103 78-79 0.9946134579929193 1103 80-81 1.0846012033825971 1103 82-83 0.9158240406659033 1103 84-85 1.065794073791924 1103 86-87 0.9996266211850369 1103 88-89 1.0153810358433653 1103 90-91 1.0035316741336828 1103 92-93 0.9800313746339242 1103 94-95 0.8754084950281396 1103 96-97 0.9530266723768008 1103 98-99 0.9582000270916673 1103 100-101 0.8414012291676762 1103 102-103 0.9535493703443727 1103 104-105 1.0648760687166856 1103 106-107 1.0155474247105296 1103 108-109 0.9537244727354768 1103 110-111 0.9747369041194709 1103 112-113 0.9423846976494623 1103 114-115 0.9430054705495046 1103 116-117 0.8891771374298223 1103 118-119 0.7653197291336156 1103 120-121 0.622668634027697 1103 122-123 0.6983603261950435 1103 124-125 0.5217787989048368 1104 1 0.8513723411633229 1104 2 0.8819846984585453 1104 3 0.9499549756218642 1104 4 0.8959724381800029 1104 5 0.930370139714995 1104 6 0.8565140646743359 1104 7 0.9759984578154857 1104 8 1.0289847162050307 1104 9 1.569355031555638 1104 10-11 1.3026923845176341 1104 12-13 1.1385576212949289 1104 14-15 1.2778202794176252 1104 16-17 1.203748994669688 1104 18-19 1.2342528160988735 1104 20-21 1.3977711707173626 1104 22-23 1.377210836069537 1104 24-25 1.5521556473706042 1104 26-27 1.3438192257311599 1104 28-29 1.3273643219729507 1104 30-31 1.4355318716293368 1104 32-33 1.353890495329594 1104 34-35 1.3831960965674739 1104 36-37 1.2941242022866604 1104 38-39 1.4412051790104314 1104 40-41 1.3091677093191834 1104 42-43 1.431363273958894 1104 44-45 1.2783495627512593 1104 46-47 1.2841442735328386 1104 48-49 1.2818590300818116 1104 50-51 1.2216490089709566 1104 52-53 1.062048894661018 1104 54-55 1.096315224946892 1104 56-57 1.1191944408470391 1104 58-59 1.1707910864921303 1104 60-61 1.3107201788463954 1104 62-63 1.3084712557300975 1104 64-65 1.28513284767142 1104 66-67 1.199325502294883 1104 68-69 1.1475246912645858 1104 70-71 1.3037809763897386 1104 72-73 1.1808845302092905 1104 74-75 1.2320135251263338 1104 76-77 1.213211323709391 1104 78-79 1.2122158760298127 1104 80-81 1.2156330494884315 1104 82-83 1.261187363541687 1104 84-85 1.1663529547855305 1104 86-87 1.229716445279788 1104 88-89 1.2140647521718826 1104 90-91 0.9976736479240245 1104 92-93 1.0977838019552522 1104 94-95 1.1045603965230129 1104 96-97 0.9785517453803507 1104 98-99 1.1571160163832914 1104 100-101 1.0340129559466185 1104 102-103 0.9982944436784642 1104 104-105 1.0552761550473733 1104 106-107 1.0001440086209428 1104 108-109 0.8438251724223313 1104 110-111 0.9963269474436025 1104 112-113 1.076634338505393 1104 114-115 1.0792151322622026 1104 116-117 0.9614147580991492 1104 118-119 0.9660539612264003 1104 120-121 0.9787212931520948 1104 122-123 1.0895035401787965 1104 124-125 0.73248845397422 1105 1 0.4474192648512343 1105 2 0.5026094311523792 1105 3 0.5276509492272901 1105 4 0.4888782853114222 1105 5 0.4039092733375256 1105 6 0.489756259334321 1105 7 0.509723942021509 1105 8 0.7362850766864852 1105 9 1.1671313497569002 1105 10-11 0.8163314142726321 1105 12-13 0.7737201434431569 1105 14-15 0.34161144456700754 1105 16-17 0.5665827455097912 1105 18-19 0.5452950046490059 1105 20-21 0.918034352729947 1105 22-23 0.8999694356907284 1105 24-25 0.7536166664944588 1105 26-27 0.834186122535705 1105 28-29 0.7619169278261921 1105 30-31 0.9539672904599072 1105 32-33 0.7914682432369631 1105 34-35 0.8056426353345003 1105 36-37 0.8142111149768354 1105 38-39 0.8676831529824511 1105 40-41 0.8035602369662769 1105 42-43 0.8269913670730915 1105 44-45 0.7576757984694709 1105 46-47 0.7554788186541259 1105 48-49 0.5102493887303154 1105 50-51 0.6508333433022386 1105 52-53 0.47326857450460835 1105 54-55 0.5115849750538075 1105 56-57 0.5760287721233865 1105 58-59 0.6933048336663177 1105 60-61 0.658839870909695 1105 62-63 0.5622234705526097 1105 64-65 0.6758768752879689 1105 66-67 0.5808253189992243 1105 68-69 0.47077834190306334 1105 70-71 0.5371827910167397 1105 72-73 0.35440029363569536 1105 74-75 0.4024858169330159 1105 76-77 0.4266163460103627 1105 78-79 0.374062427016554 1105 80-81 0.33261951615916985 1105 82-83 0.396349885689915 1105 84-85 0.5880006299856291 1105 86-87 0.6198221457746875 1105 88-89 0.5308549397444402 1105 90-91 0.5117588498547363 1105 92-93 0.5390818406917361 1105 94-95 0.5921631948366937 1105 96-97 0.5170826306983685 1105 98-99 0.45636419870137246 1105 100-101 0.40190612512850876 1105 102-103 0.32421840653054446 1105 104-105 0.33591387118350013 1105 106-107 0.3635951603286429 1105 108-109 0.32774433903808387 1105 110-111 0.384499795247109 1105 112-113 0.4115784333303445 1105 114-115 0.3268281645833362 1105 116-117 0.32934794683257707 1105 118-119 0.08713586383530725 1105 120-121 0.23263160047781284 1105 122-123 0.19573055184861943 1105 124-125 0.09052719045616797 1106 1 0.8546574348445048 1106 2 0.849821810609658 1106 3 0.9349339723266397 1106 4 0.8929924364663933 1106 5 0.7506058420749113 1106 6 0.7972411214110195 1106 7 0.7953608561278145 1106 8 0.912525265069533 1106 9 1.4811064562830296 1106 10-11 0.8640248432925901 1106 12-13 1.100663404263969 1106 14-15 1.2494910139771847 1106 16-17 1.0932423104346611 1106 18-19 1.155977332525211 1106 20-21 1.2359486832973374 1106 22-23 1.2182207252175274 1106 24-25 1.1078580883379132 1106 26-27 1.2530339583733294 1106 28-29 1.1526110296427419 1106 30-31 1.2342930361427946 1106 32-33 1.2656255655065323 1106 34-35 1.2028051157112714 1106 36-37 1.2340866476074766 1106 38-39 1.506107126967656 1106 40-41 1.3604238835238007 1106 42-43 1.214664612195314 1106 44-45 1.0416206291489942 1106 46-47 1.1698424652116515 1106 48-49 1.0432993999435922 1106 50-51 1.032663350030198 1106 52-53 0.9572778815241207 1106 54-55 0.7984480609475071 1106 56-57 0.7348474534420646 1106 58-59 0.8414120325898438 1106 60-61 0.9531379197995804 1106 62-63 1.0341734593393284 1106 64-65 1.110295466900439 1106 66-67 1.0445189722847203 1106 68-69 1.024137278884453 1106 70-71 0.9546418625574447 1106 72-73 1.047999755398422 1106 74-75 1.091025287666362 1106 76-77 1.0100487299529526 1106 78-79 0.9803620457651547 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-2.014603722338908 2216 122-123 -1.9156895977692017 2216 124-125 -1.8311495049414575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55586.0 3 10313.0 4 6544.0 5 5640.0 6 5820.0 7 6647.0 8 7395.0 9 7967.0 10 8447.0 11 9065.0 12 9547.0 13 9688.0 14 9990.0 15 9496.0 16 8881.0 17 8274.0 18 7750.0 19 7455.0 20 7191.0 21 7728.0 22 8943.0 23 11579.0 24 16429.0 25 22041.0 26 25233.0 27 27734.0 28 32734.0 29 37876.0 30 44596.0 31 52574.0 32 65051.0 33 82567.0 34 109400.0 35 157041.0 36 268122.0 37 386792.0 38 126529.0 39 201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86303322941222 18.555029085240665 12.360455003421793 24.22148268192533 2 15.630994549114943 20.330186887487656 39.23610451644762 24.802714046949784 3 18.52812033716628 25.50968444968324 29.353432261081892 26.60876295206859 4 12.212131589865157 16.330739814617523 37.075011826041745 34.38211676947558 5 12.937554567269313 39.64834789152962 34.20571719568602 13.208380345515053 6 32.22592249449658 36.84232705249869 17.43805572091704 13.493694732087691 7 28.78222956603077 33.32104748983005 20.785332483414113 17.11139046072507 8 27.044800852784313 35.21990890497224 19.896915660533914 17.838374581709534 9 27.331648118021256 16.070876381206862 19.350101997270972 37.24737350350091 10-11 24.69468355091585 27.27290804550283 27.65627314043295 20.376135263148363 12-13 25.30512858179422 24.78694343172649 27.519397443944392 22.388530542534895 14-15 22.39875069519586 26.032762792939362 24.814886480525317 26.75360003133946 16-17 22.61104580542096 29.501193456953494 25.73804250505175 22.149718232573793 18-19 22.85402387959763 28.059239542733128 27.0529876802931 22.03374889737614 20-21 23.369415357235187 27.295082964711764 26.99458576710636 22.340915910946688 22-23 23.196217446378604 27.307230682110927 26.942117266047838 22.554434605462635 24-25 22.88077014283768 27.556183701955483 26.891485565906052 22.671560589300782 26-27 22.57474857257843 27.86328687745633 26.568887522326186 22.99307702763906 28-29 22.781945961346693 27.87089249252101 26.63973835414787 22.707423191984425 30-31 22.71568873053319 27.442693350208426 26.735079256340327 23.10653866291805 32-33 22.39962916073876 27.71723743743423 26.62558232981784 23.257551072009175 34-35 23.245281607841207 27.547729966531005 26.44543141408428 22.76155701154351 36-37 23.312839922592538 27.089851592173904 26.414213011777605 23.183095473455953 38-39 22.809073524297002 27.302063437987055 26.64512380271809 23.243739234997847 40-41 22.969585025889884 27.22628066485125 26.84915018031514 22.954984128943725 42-43 22.870378751344735 27.08645620803502 26.6371480505991 23.406016990021143 44-45 22.736101445684092 27.335908252551793 26.86470693848238 23.06328336328173 46-47 23.273784192679724 27.518789762984568 26.177198014989077 23.030228029346635 48-49 22.903880078921688 27.5750159671587 26.25190834257331 23.269195611346298 50-51 23.027973435059952 27.4190293433493 26.51623924115773 23.036757980433016 52-53 23.308645785878685 27.050330247266242 26.57829405139422 23.062729915460856 54-55 22.59729675666596 27.35919445224331 26.735083696549626 23.308425094541104 56-57 22.777577772349765 27.67246411996606 26.537261011238655 23.01269709644552 58-59 22.863098962074094 26.991059567663438 26.624942274794535 23.520899195467933 60-61 22.415846603190584 27.297970856719378 26.992897449450975 23.293285090639063 62-63 22.57253292485627 27.224707599258352 27.14546178199802 23.057297693887357 64-65 22.39993779361429 27.222475974415843 26.78854488401447 23.589041347955394 66-67 22.35365455865972 27.51282721759419 26.64778584133993 23.485732382406166 68-69 22.70683931002019 26.882120633088945 27.092048160158704 23.318991896732157 70-71 22.5081234183855 27.009071459669 26.84605406194661 23.63675105999889 72-73 22.452102685502574 27.06201608137624 26.813384268673136 23.672496964448055 74-75 22.404421375800325 27.390400526167703 26.75411988598117 23.451058212050807 76-77 22.61914089163506 27.107528030532357 26.930738320364206 23.342592757468378 78-79 22.70942643397442 27.04247514935023 26.971483892374643 23.276614524300705 80-81 22.762999652147485 27.28911886464738 26.719258088758192 23.22862339444694 82-83 22.65005262504905 27.184295711815377 26.90496444470036 23.260687218435212 84-85 22.521502438659542 27.211243744562992 26.940799324719993 23.326454492057476 86-87 22.698592763291458 27.145403774713905 26.76035185073057 23.395651611264068 88-89 22.564921910872226 27.137541282508444 26.738777553950342 23.558759252668988 90-91 22.50514798719488 27.227020150904735 26.87679674992684 23.391035111973544 92-93 22.720215919171583 26.767008694996658 27.055634979309428 23.45714040652233 94-95 22.37164895369301 26.56957919197975 27.3351298572179 23.723641997109336 96-97 22.648760032563633 26.35410486664848 27.274594342083745 23.722540758704138 98-99 22.52727766458971 26.717020105267373 26.856149920685912 23.899552309456997 100-101 22.186267476988945 26.568287914704253 27.343064363280966 23.902380245025835 102-103 22.2030194888452 26.234257913781807 27.482702700135814 24.080019897237182 104-105 21.942838385580167 26.59458371755577 27.337900176264977 24.12467772059908 106-107 22.205480610711035 26.49449549861033 27.012285150830312 24.287738739848315 108-109 22.271684121276223 26.396747148101092 26.986253845786308 24.345314884836377 110-111 22.073030991829352 26.64952466255389 27.261366000484937 24.016078345131817 112-113 22.100179319866637 26.757747229623806 27.390501211195588 23.751572239313965 114-115 21.903116487328603 26.96820234343511 27.1800007241985 23.948680445037787 116-117 21.900985514816853 26.943598160451486 27.28138295583214 23.87403336889952 118-119 21.675205469592523 27.09884727647579 26.960667971726483 24.265279282205206 120-121 21.703498947988354 27.209699404889122 27.132787379659838 23.954014267462682 122-123 21.776486691310904 27.141699015566356 27.20112778104804 23.880686512074696 124-125 21.685972509067415 27.17886418525042 27.125886030633335 24.009277275048834 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 834.0 1 676.5 2 472.0 3 364.5 4 271.5 5 234.0 6 214.5 7 203.5 8 212.0 9 211.5 10 198.0 11 193.5 12 205.0 13 218.0 14 233.0 15 264.0 16 307.0 17 373.0 18 441.5 19 526.5 20 725.5 21 1046.0 22 1435.5 23 2027.0 24 2869.0 25 3974.5 26 5591.5 27 7881.0 28 10345.5 29 13253.0 30 17106.5 31 21613.5 32 27914.5 33 31955.5 34 33712.5 35 36338.0 36 40654.0 37 45888.5 38 48570.0 39 50230.5 40 51639.0 41 54019.5 42 55559.5 43 56481.0 44 58905.5 45 62537.5 46 63910.0 47 63567.0 48 63400.0 49 61990.5 50 61105.0 51 59927.5 52 59187.0 53 60123.0 54 60806.0 55 57700.0 56 52438.5 57 47620.0 58 43854.0 59 38339.0 60 30674.5 61 25366.5 62 21840.0 63 17025.5 64 12241.5 65 9243.5 66 6848.5 67 5515.0 68 4160.5 69 2734.0 70 1962.0 71 1322.0 72 1002.0 73 746.0 74 533.5 75 411.5 76 302.5 77 175.0 78 87.0 79 55.5 80 32.5 81 24.0 82 17.5 83 10.5 84 11.5 85 10.5 86 6.0 87 5.5 88 5.5 89 3.5 90 2.0 91 2.0 92 0.5 93 0.5 94 1.0 95 0.5 96 1.5 97 2.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09128322371037222 2 1.187038019640731E-4 3 0.0 4 0.0014837975245509138 5 0.0011276861186586946 6 2.967595049101828E-4 7 0.0 8 0.0018992608314251697 9 0.0010089823166946213 10-11 0.010653666226275562 12-13 0.00258180769271859 14-15 0.005282319187401253 16-17 0.0032346786035209922 18-19 0.014719271443545064 20-21 0.01925969186867086 22-23 0.010920749780694726 24-25 0.01136588903806 26-27 0.010980101681676763 28-29 0.008665377543377336 30-31 0.019704831126036135 32-33 0.0023147241382994255 34-35 0.0013650937225868408 36-37 9.199544652215665E-4 38-39 4.748152078562924E-4 40-41 0.002255372237317389 42-43 0.003175326702538956 44-45 0.001394769673077859 46-47 0.0023147241382994255 48-49 0.009377600355161777 50-51 0.005282319187401253 52-53 0.008516997790922246 54-55 0.008843433246323446 56-57 0.011247185236095927 58-59 0.00949630415712585 60-61 0.011247185236095927 62-63 0.013918020780287572 64-65 0.008991812998778537 66-67 0.015698577809748666 68-69 0.014066400532742662 70-71 0.004540420425125796 72-73 0.01463024359207201 74-75 0.01376964102783248 76-77 0.005697782494275509 78-79 0.008991812998778537 80-81 0.014481863839616919 82-83 0.018221033601485224 84-85 0.013739965077341461 86-87 0.017093347482826527 88-89 0.0168856158293894 90-91 0.013472881522922297 92-93 0.009585332008598903 94-95 0.0077750990286467895 96-97 0.009763387711545012 98-99 0.006439681256550965 100-101 0.011069129533149818 102-103 0.012463899206227674 104-105 0.011069129533149818 106-107 0.006439681256550965 108-109 0.004006253316287467 110-111 0.006855144563425223 112-113 0.009911767464000104 114-115 0.014541215740598957 116-117 0.012671630859664804 118-119 0.2206110159502299 120-121 0.028132801065485324 122-123 0.029468218837581148 124-125 0.23648764946292467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1684866.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.04231827628435 #Duplication Level Percentage of deduplicated Percentage of total 1 86.52512632148671 63.1999581566974 2 8.181356685791041 11.951705179507123 3 2.4137133042365355 5.289096461872409 4 1.0395273431921388 3.0371794823336185 5 0.5355494462775904 1.9558886553847805 6 0.31104157133734683 1.3631518470466855 7 0.19841885303244267 1.0145081110647676 8 0.14041034144806513 0.8204717479465065 9 0.09787849544329902 0.6434344994915966 >10 0.49982132938426393 6.771975278139 >50 0.04307145437619797 2.1579235095662495 >100 0.014084853994344835 1.7947070709498796 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.935190098203655E-5 0.0 14-15 0.0 0.0 0.0 5.935190098203655E-5 0.0 16-17 0.0 0.0 0.0 5.935190098203655E-5 0.0 18-19 0.0 0.0 0.0 8.902785147305482E-5 0.0 20-21 0.0 0.0 0.0 2.0773165343712795E-4 0.0 22-23 0.0 0.0 0.0 4.4513925736527416E-4 0.0 24-25 0.0 0.0 0.0 8.309266137485117E-4 0.0 26-27 0.0 0.0 0.0 0.0013057418216048042 0.0 28-29 0.0 0.0 0.0 0.003739169761868303 0.0 30-31 0.0 0.0 0.0 0.015016030948455248 0.0 32-33 0.0 0.0 0.0 0.03846003183635969 0.0 34-35 0.0 0.0 0.0 0.07282478250495886 0.0 36-37 0.0 0.0 0.0 0.12757691116088757 0.0 38-39 0.0 0.0 0.0 0.21307332452551123 0.0 40-41 0.0 0.0 0.0 0.3056029381565062 0.0 42-43 0.0 0.0 0.0 0.3952243086393814 0.0 44-45 0.0 0.0 0.0 0.5054704647135143 0.0 46-47 0.0 0.0 0.0 0.6408521508535396 0.0 48-49 0.0 0.0 0.0 0.7845431031310501 0.0 50-51 0.0 0.0 0.0 0.9492149524057106 0.0 52-53 0.0 0.0 0.0 1.101185494870215 0.0 54-55 0.0 0.0 0.0 1.254966270314672 0.0 56-57 0.0 0.0 0.0 1.4375623936859074 0.0 58-59 0.0 0.0 0.0 1.6406645988464366 0.0 60-61 0.0 0.0 0.0 1.8761135900421755 0.0 62-63 0.0 0.0 0.0 2.102452064437172 0.0 64-65 0.0 0.0 0.0 2.3279002603174375 0.0 66-67 0.0 0.0 0.0 2.5841520928073805 0.0 68-69 0.0 0.0 0.0 2.8865203523603657 0.0 70-71 0.0 0.0 0.0 3.2109675190786686 0.0 72-73 0.0 0.0 0.0 3.541438903746648 0.0 74-75 0.0 0.0 0.0 3.8637790779800887 0.0 76-77 0.0 0.0 0.0 4.209919364507326 0.0 78-79 0.0 0.0 0.0 4.606538442819785 0.0 80-81 0.0 0.0 0.0 5.042804590988245 0.0 82-83 0.0 0.0 0.0 5.498716218381759 0.0 84-85 0.0 0.0 0.0 5.944567698558817 0.0 86-87 0.0 0.0 0.0 6.396740156190463 0.0 88-89 0.0 0.0 0.0 6.88511727342115 0.0 90-91 0.0 0.0 0.0 7.433914625851552 0.0 92-93 0.0 0.0 0.0 8.004612829744325 0.0 94-95 0.0 0.0 0.0 8.56492445096524 0.0 96-97 0.0 0.0 0.0 9.122535560691473 0.0 98-99 0.0 0.0 0.0 9.714184985630904 0.0 100-101 0.0 0.0 0.0 10.369608028175534 0.0 102-103 0.0 0.0 0.0 11.076993660029938 0.0 104-105 0.0 0.0 0.0 11.774586228222304 0.0 106-107 0.0 0.0 0.0 12.456509894555413 0.0 108-109 0.0 0.0 0.0 13.162441404835755 0.0 110-111 0.0 0.0 0.0 13.921641246247475 0.0 112-113 0.0 0.0 0.0 14.712386623031149 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1780 0.0 54.85034 1 GTATCAA 2960 0.0 52.091103 1 TCAACGC 3560 0.0 41.771923 4 ATCAACG 3580 0.0 41.703476 3 CAACGCA 3630 0.0 40.967617 5 AACGCAG 3785 0.0 39.28995 6 GTACATG 8825 0.0 38.856464 1 TATCAAC 3990 0.0 37.865387 2 TACATGG 9155 0.0 36.44904 2 ACATGGG 9215 0.0 35.43713 3 CATGGGA 6625 0.0 33.400387 4 ACGCAGA 4640 0.0 32.1783 7 CGCAGAG 4645 0.0 32.0156 8 ATGGGAG 2890 0.0 27.581274 5 GAGTACA 6435 0.0 25.626347 1 CATGGGG 3360 0.0 25.138739 4 GCAGAGT 5705 0.0 24.92083 9 GTGGTAT 1055 0.0 24.8288 1 ATGGGAT 2925 0.0 24.607462 5 AGAGTAC 4945 0.0 22.555643 10-11 >>END_MODULE