FastQCFastQC Report
Thu 26 May 2016
SRR1364434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684866
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.1741978293822773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26400.1566890185925765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.071.292551
GTATCAA49300.049.063691
ATCAACG63850.037.7651633
TCAACGC64700.037.2679144
GTACATG86850.037.11151
CAACGCA66700.036.128985
TATCAAC67900.035.3424842
AACGCAG68850.035.087196
GTGGTAT13600.035.0454941
TGGTATC13350.034.7916562
TACATGG91150.034.5589682
ACATGGG90900.033.7318843
CATGGGA65450.030.109444
ACGCAGA84150.028.6369927
CGCAGAG86100.027.9168268
TAAGGTG11650.027.5800045
CATGGGG33550.026.4410134
GAGTACT52000.025.05697412-13
GAGTACA66350.024.603151
ATGGGAT28500.024.00935
Produced by FastQC (version 0.11.5)