##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364434_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1684866 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92711467855604 33.0 31.0 34.0 30.0 34.0 2 31.84708338823384 33.0 31.0 34.0 30.0 34.0 3 32.062524260089525 33.0 31.0 34.0 30.0 34.0 4 35.76879170212943 37.0 35.0 37.0 35.0 37.0 5 35.71379148252739 37.0 35.0 37.0 33.0 37.0 6 35.78063240637535 37.0 35.0 37.0 33.0 37.0 7 35.67907477508597 37.0 35.0 37.0 33.0 37.0 8 35.68479807889767 37.0 35.0 37.0 33.0 37.0 9 37.31801104657581 39.0 37.0 39.0 34.0 39.0 10-11 37.27587742882817 39.0 37.0 39.0 33.5 39.0 12-13 37.25936572997496 39.0 37.0 39.0 33.0 39.0 14-15 38.55420490412887 40.0 38.0 41.0 34.0 41.0 16-17 38.57104927038708 40.0 38.0 41.0 34.0 41.0 18-19 38.63169533957003 40.0 38.0 41.0 34.0 41.0 20-21 38.63543718016744 40.0 38.0 41.0 34.0 41.0 22-23 38.610876473262564 40.0 38.0 41.0 34.0 41.0 24-25 38.556412201326395 40.0 38.0 41.0 34.0 41.0 26-27 38.44687055231692 40.0 38.0 41.0 34.0 41.0 28-29 38.36499964982379 40.0 38.0 41.0 34.0 41.0 30-31 38.23137507671233 40.0 38.0 41.0 33.0 41.0 32-33 38.09877254333579 40.0 37.0 41.0 33.0 41.0 34-35 37.999650417303215 40.0 37.0 41.0 33.0 41.0 36-37 37.889753547166364 40.0 37.0 41.0 33.0 41.0 38-39 37.78219692248523 40.0 37.0 41.0 33.0 41.0 40-41 37.65120341914431 40.0 37.0 41.0 32.5 41.0 42-43 37.50249812151233 40.0 37.0 41.0 32.0 41.0 44-45 37.320268496129664 40.0 36.5 41.0 31.0 41.0 46-47 37.09427218544383 40.0 36.0 41.0 31.0 41.0 48-49 36.84441195917064 40.0 36.0 41.0 30.5 41.0 50-51 36.62079417591666 39.5 35.5 41.0 30.0 41.0 52-53 36.47768605930679 39.0 35.0 41.0 30.0 41.0 54-55 36.32295357613009 39.0 35.0 41.0 30.0 41.0 56-57 36.108792924778584 39.0 35.0 41.0 29.0 41.0 58-59 35.878642871302524 38.5 35.0 40.0 29.0 41.0 60-61 35.632393021166074 38.0 35.0 40.0 28.5 41.0 62-63 35.66828964439902 38.0 35.0 40.0 29.0 41.0 64-65 35.44793117078747 37.0 35.0 40.0 29.0 41.0 66-67 35.17335147127427 37.0 34.0 40.0 29.0 41.0 68-69 34.849281485886706 36.5 34.0 39.0 29.0 41.0 70-71 34.50580431915654 36.0 34.0 39.0 28.0 41.0 72-73 34.165677270477296 35.5 34.0 39.0 28.0 40.5 74-75 33.83647097157875 35.0 34.0 37.5 28.0 40.0 76-77 33.523894778575865 35.0 33.5 37.0 28.0 39.0 78-79 33.203948860028035 35.0 33.0 36.5 27.0 39.0 80-81 32.92339835927605 35.0 33.0 36.0 27.0 38.5 82-83 32.65402382147898 35.0 33.0 36.0 26.0 37.0 84-85 32.437850250405674 35.0 33.0 35.0 26.0 37.0 86-87 32.260426645205015 35.0 33.0 35.0 26.0 36.5 88-89 32.080880022506236 35.0 33.0 35.0 26.0 36.0 90-91 31.955738913361657 35.0 33.0 35.0 26.0 36.0 92-93 31.842046785916505 35.0 33.0 35.0 25.5 36.0 94-95 31.729510239983476 35.0 33.0 35.0 25.0 35.0 96-97 31.67570833526227 35.0 33.0 35.0 25.0 35.0 98-99 31.594906657265327 35.0 32.5 35.0 25.0 35.0 100-101 31.58293804967279 35.0 32.5 35.0 25.0 35.0 102-103 30.463323196028647 33.5 30.0 34.5 23.5 35.0 104-105 31.425011840704244 35.0 32.5 35.0 25.0 35.0 106-107 31.609668365318072 35.0 33.0 35.0 25.0 35.0 108-109 31.615621064227067 35.0 33.0 35.0 25.0 35.0 110-111 31.55235995028685 35.0 33.0 35.0 25.0 35.0 112-113 31.49234063717827 35.0 33.0 35.0 25.0 35.0 114-115 31.37704452460908 35.0 33.0 35.0 24.5 35.0 116-117 31.28602482333907 35.0 33.0 35.0 24.0 35.0 118-119 31.178097546036305 35.0 33.0 35.0 24.0 35.0 120-121 31.04886263952148 35.0 33.0 35.0 23.5 35.0 122-123 30.861761706865707 35.0 33.0 35.0 21.5 35.0 124-125 29.836876345062457 34.5 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32394950540509626 1101 2 0.4880577718240353 1101 3 0.31186803946051356 1101 4 0.22434951382979307 1101 5 0.29559566906029033 1101 6 0.2851516144677788 1101 7 0.34804029412971005 1101 8 0.3162388060324588 1101 9 0.4344161809136651 1101 10-11 0.4077989855206212 1101 12-13 0.4078460747345716 1101 14-15 0.45261059255657443 1101 16-17 0.455558912811469 1101 18-19 0.3631613763472856 1101 20-21 0.360169565650061 1101 22-23 0.45703461502994003 1101 24-25 0.4088374725498838 1101 26-27 0.3518771096700277 1101 28-29 0.420398927675123 1101 30-31 0.27790592970949746 1101 32-33 0.35053392852901055 1101 34-35 0.2890232107180992 1101 36-37 0.3698047114046332 1101 38-39 0.26759221768438124 1101 40-41 0.25308898176141525 1101 42-43 0.26590515592361896 1101 44-45 0.3255808310210213 1101 46-47 0.4005914511905928 1101 48-49 0.28417264175369894 1101 50-51 0.30641225769694813 1101 52-53 0.13504177884713187 1101 54-55 0.3069299232491929 1101 56-57 0.29243519676009555 1101 58-59 0.2253378712496854 1101 60-61 0.21311716055182472 1101 62-63 0.440012443045255 1101 64-65 0.38591051229126805 1101 66-67 0.3131913606845984 1101 68-69 0.4163124972053467 1101 70-71 0.43901301662087633 1101 72-73 0.4803439341186291 1101 74-75 0.41660611982199214 1101 76-77 0.4469478740996209 1101 78-79 0.3576020067675856 1101 80-81 0.34579477061828356 1101 82-83 0.33902503817669327 1101 84-85 0.325575747946921 1101 86-87 0.354481331521761 1101 88-89 0.40187818461602376 1101 90-91 0.37183799410207996 1101 92-93 0.3489124867795361 1101 94-95 0.36403950585641454 1101 96-97 0.2456538936202648 1101 98-99 0.3193630403996828 1101 100-101 0.31687389155447576 1101 102-103 0.37416252023898267 1101 104-105 0.39627679116513903 1101 106-107 0.46898643661483774 1101 108-109 0.4693670641753407 1101 110-111 0.5464326659105687 1101 112-113 0.5827217571574472 1101 114-115 0.6215010806274535 1101 116-117 0.5148493681281927 1101 118-119 0.5244644066264392 1101 120-121 0.5077868212993728 1101 122-123 0.6081926099218116 1101 124-125 0.540184172127038 1102 1 0.3132562180749261 1102 2 0.4621870372462844 1102 3 0.2908587967174725 1102 4 0.22190237203217578 1102 5 0.2549301081458921 1102 6 0.262222769263623 1102 7 0.28982283005406373 1102 8 0.296136701254774 1102 9 0.37297938196977043 1102 10-11 0.3363066007647646 1102 12-13 0.33428826240740506 1102 14-15 0.3668136711739862 1102 16-17 0.42961511456451973 1102 18-19 0.340989159461067 1102 20-21 0.43576326274124 1102 22-23 0.34678320045081534 1102 24-25 0.3651991194276647 1102 26-27 0.27240008233832924 1102 28-29 0.3586599760805953 1102 30-31 0.38890309239108234 1102 32-33 0.3994837912871816 1102 34-35 0.39842846187275427 1102 36-37 0.3544277935399833 1102 38-39 0.20091024819613068 1102 40-41 0.311816002595215 1102 42-43 0.40268502921745863 1102 44-45 0.37559574598306966 1102 46-47 0.31304878412336734 1102 48-49 0.15227926967873628 1102 50-51 0.2348104663299182 1102 52-53 0.13116351092907763 1102 54-55 0.16531334083367 1102 56-57 0.24228443021869595 1102 58-59 0.24248441082738026 1102 60-61 0.2699263922937689 1102 62-63 0.2945682159364722 1102 64-65 0.33498709933692083 1102 66-67 0.33413426433605764 1102 68-69 0.37469559745021286 1102 70-71 0.4348189957828481 1102 72-73 0.4323833398412589 1102 74-75 0.2974097325370977 1102 76-77 0.36803246233483833 1102 78-79 0.30884889457261266 1102 80-81 0.29285987782769496 1102 82-83 0.31284716418289094 1102 84-85 0.2820432502544321 1102 86-87 0.3196115253890639 1102 88-89 0.34272879986198745 1102 90-91 0.26579654289893995 1102 92-93 0.28727092822493816 1102 94-95 0.32927631072398 1102 96-97 0.2175394923857219 1102 98-99 0.19282001428535267 1102 100-101 0.10564750388877187 1102 102-103 0.07663585011079022 1102 104-105 0.10421946935435855 1102 106-107 0.16514334806015896 1102 108-109 0.13779808874710042 1102 110-111 0.06874987677941746 1102 112-113 0.11417676232857232 1102 114-115 0.13007091257043157 1102 116-117 0.15217476333690882 1102 118-119 0.09845576531752442 1102 120-121 0.07960886147275303 1102 122-123 0.08503218235351895 1102 124-125 -0.1722505239129859 1103 1 0.22612857815632736 1103 2 0.3966805442966823 1103 3 0.23430158920604072 1103 4 0.15773180038902268 1103 5 0.0984635175293107 1103 6 0.15377698458878797 1103 7 0.13558824012861947 1103 8 0.21508944236854433 1103 9 0.260870612688457 1103 10-11 0.2372084816486435 1103 12-13 0.26184204135893907 1103 14-15 0.3339427393964627 1103 16-17 0.28632068595857874 1103 18-19 0.1952241277451563 1103 20-21 0.23463860744173104 1103 22-23 0.2635461120971314 1103 24-25 0.3336242236497 1103 26-27 0.3032040723824494 1103 28-29 0.37687124460992294 1103 30-31 0.36118162173626445 1103 32-33 0.29057396550241776 1103 34-35 0.3145569133706516 1103 36-37 0.28153684343729424 1103 38-39 0.2507292035857489 1103 40-41 0.16785101212039422 1103 42-43 0.24546518960369212 1103 44-45 0.1086846123385996 1103 46-47 0.029227620380467556 1103 48-49 0.026286393541376185 1103 50-51 -0.010773930664150555 1103 52-53 0.06420719495673666 1103 54-55 -0.11719066188546634 1103 56-57 -0.0727974339795594 1103 58-59 -0.19796895426285488 1103 60-61 -0.22094349836689275 1103 62-63 0.04913082703991023 1103 64-65 0.1470181316821666 1103 66-67 0.021431251277007846 1103 68-69 0.08883169116207768 1103 70-71 -0.033584904590270526 1103 72-73 -0.004929569692556868 1103 74-75 0.04597275794323963 1103 76-77 0.043915630230856095 1103 78-79 -0.02929282533226285 1103 80-81 0.023411849486109304 1103 82-83 -0.08797218722211397 1103 84-85 -0.023384083761200714 1103 86-87 -0.00390742123525456 1103 88-89 0.04175963419955053 1103 90-91 -0.07656174760104761 1103 92-93 -0.16887478273302392 1103 94-95 -0.045411306921909755 1103 96-97 0.029673154512718014 1103 98-99 0.0028470070214972054 1103 100-101 0.03537999802263769 1103 102-103 0.03120041606721813 1103 104-105 0.10605293227552437 1103 106-107 -0.0036690062323536665 1103 108-109 -0.022109043124128647 1103 110-111 -0.10856971393742754 1103 112-113 -0.1419329414985917 1103 114-115 -0.02268555625880353 1103 116-117 -0.003170939801638184 1103 118-119 -0.009904731013342882 1103 120-121 -0.09786191505160602 1103 122-123 -0.12706944540842002 1103 124-125 -0.41429411461217214 1104 1 0.36630640510543344 1104 2 0.43544541924519464 1104 3 0.2655243576923638 1104 4 0.1739766489846417 1104 5 0.22621721194137479 1104 6 0.20848111566106553 1104 7 0.2562368285538952 1104 8 0.25826839479236696 1104 9 0.3287503810085326 1104 10-11 0.34919985895034955 1104 12-13 0.3515012684854426 1104 14-15 0.38591217431613245 1104 16-17 0.4036559617322908 1104 18-19 0.2881900242086104 1104 20-21 0.25063789644503487 1104 22-23 0.25957754178055836 1104 24-25 0.3008281238262853 1104 26-27 0.23230929243981535 1104 28-29 0.32505233747730244 1104 30-31 0.26566291011153 1104 32-33 0.22851430788912808 1104 34-35 0.2443514178263655 1104 36-37 0.1165510163818908 1104 38-39 0.17063027328903502 1104 40-41 0.19217356416427833 1104 42-43 0.19703372479170866 1104 44-45 0.2561247163761493 1104 46-47 0.35981224178769367 1104 48-49 0.11447526070716663 1104 50-51 0.27324348583829305 1104 52-53 0.20665444298796132 1104 54-55 0.2310394942907621 1104 56-57 0.3773568853577629 1104 58-59 0.21473505625755962 1104 60-61 0.2391507589251134 1104 62-63 0.4889244921695379 1104 64-65 0.5427080463852505 1104 66-67 0.4972620625812709 1104 68-69 0.49542004449178734 1104 70-71 0.4299980650620938 1104 72-73 0.40654955944343385 1104 74-75 0.4350376676638419 1104 76-77 0.43420209967857915 1104 78-79 0.43925602198783764 1104 80-81 0.42385627103002577 1104 82-83 0.30648109877193264 1104 84-85 0.29127845692099186 1104 86-87 0.3880714731162769 1104 88-89 0.4903773224621659 1104 90-91 0.4310116310494152 1104 92-93 0.44400979631901905 1104 94-95 0.48262720745146837 1104 96-97 0.5091777187939108 1104 98-99 0.4618242962834067 1104 100-101 0.4291814290250109 1104 102-103 0.3788124199209868 1104 104-105 0.47526202784819205 1104 106-107 0.480087036348376 1104 108-109 0.4486250496210822 1104 110-111 0.5702928631047648 1104 112-113 0.36181785772456365 1104 114-115 0.36769019285593174 1104 116-117 0.30201369735510397 1104 118-119 0.21616037865481985 1104 120-121 0.307248219762311 1104 122-123 0.2534131930180017 1104 124-125 0.0934773482359823 1105 1 0.20293223757320078 1105 2 0.3515937665293656 1105 3 0.1808011341327571 1105 4 0.14816678767835612 1105 5 0.13354323381465605 1105 6 0.14698022973205838 1105 7 0.15960763566576475 1105 8 0.1173797163540442 1105 9 0.13060808248100386 1105 10-11 0.17042977280139127 1105 12-13 0.21063382179392676 1105 14-15 0.25085718561935977 1105 16-17 0.251177651718244 1105 18-19 0.27099124006979736 1105 20-21 0.19897066970830934 1105 22-23 0.2166348827715865 1105 24-25 0.2781333721918955 1105 26-27 0.22108900161070721 1105 28-29 0.19963518711376338 1105 30-31 0.18169883606008597 1105 32-33 0.2163135380473733 1105 34-35 0.19687127485575928 1105 36-37 0.20622276441145715 1105 38-39 0.16413519172246538 1105 40-41 0.10956389226350183 1105 42-43 0.1614375556709149 1105 44-45 0.17052946373362943 1105 46-47 0.17301579161356528 1105 48-49 0.1229676539374509 1105 50-51 0.20638578436582122 1105 52-53 0.058806181241770616 1105 54-55 -0.00801622758863374 1105 56-57 -0.1975086978279066 1105 58-59 -0.09202397105348581 1105 60-61 -0.06855208826740267 1105 62-63 0.13557552778999593 1105 64-65 0.17837997771047753 1105 66-67 0.19940860745815314 1105 68-69 0.2518175211215947 1105 70-71 0.2559270685447075 1105 72-73 0.18795253494291586 1105 74-75 0.19242310787890915 1105 76-77 0.14339300210419026 1105 78-79 0.12132481895735481 1105 80-81 0.20056764147054196 1105 82-83 0.20134912589968934 1105 84-85 0.15456494803115106 1105 86-87 0.1577511639576059 1105 88-89 0.25410051547249424 1105 90-91 0.13942215222006382 1105 92-93 0.1519246554868552 1105 94-95 0.0384925156936653 1105 96-97 0.1723079503574283 1105 98-99 0.23923631436857917 1105 100-101 0.26846672871340616 1105 102-103 0.27875361256472075 1105 104-105 0.2635248432694688 1105 106-107 0.27158104869028676 1105 108-109 0.26759508078134076 1105 110-111 0.32047172911564203 1105 112-113 0.37326070002920275 1105 114-115 0.18036814238853083 1105 116-117 0.24818831701661637 1105 118-119 0.24310652028126256 1105 120-121 0.2965355051536456 1105 122-123 0.30029701412144405 1105 124-125 0.22089654540100767 1106 1 0.2899506273466983 1106 2 0.4348355247711275 1106 3 0.19284531444224484 1106 4 0.1419961216097292 1106 5 0.10264280771015422 1106 6 0.1375151030220323 1106 7 0.13520418197659723 1106 8 0.055252557798318946 1106 9 0.23225419147405546 1106 10-11 0.2741211834315749 1106 12-13 0.247910677591193 1106 14-15 0.36888028496898784 1106 16-17 0.24113616259511872 1106 18-19 0.22567222235281292 1106 20-21 0.2332889024959286 1106 22-23 0.2540305992999521 1106 24-25 0.1455117081416617 1106 26-27 0.20632335908995714 1106 28-29 0.2735514239381658 1106 30-31 0.20923696120025426 1106 32-33 0.32727003053447845 1106 34-35 0.2815797296662481 1106 36-37 0.2214722598140142 1106 38-39 0.20965773040826718 1106 40-41 0.10094817124982569 1106 42-43 0.1590025926747245 1106 44-45 0.06319037425168972 1106 46-47 0.22171673352878685 1106 48-49 0.13998604371093393 1106 50-51 0.19098603105790346 1106 52-53 0.07898503300225457 1106 54-55 -0.0595552497909182 1106 56-57 0.03127578299739042 1106 58-59 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-0.7571068685243816 2216 86-87 -0.7243406395876875 2216 88-89 -0.6233677720539319 2216 90-91 -0.7160966313219781 2216 92-93 -0.8431635702409039 2216 94-95 -0.7214656075784127 2216 96-97 -0.6682868618071538 2216 98-99 -0.7035214511217482 2216 100-101 -0.7527769694488988 2216 102-103 -0.7402027217985889 2216 104-105 -0.7248769293764887 2216 106-107 -0.8134535097745044 2216 108-109 -0.7081778091194586 2216 110-111 -0.8442286286661123 2216 112-113 -0.7474816971085438 2216 114-115 -0.9011247832904417 2216 116-117 -0.825755413671299 2216 118-119 -0.9510864379233226 2216 120-121 -1.0820390598162675 2216 122-123 -1.0254522947092646 2216 124-125 -0.964522221878223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69.0 3 16.0 4 46.0 5 80.0 6 321.0 7 709.0 8 1233.0 9 2172.0 10 3243.0 11 4497.0 12 5896.0 13 7994.0 14 11292.0 15 8894.0 16 4911.0 17 4445.0 18 4562.0 19 4505.0 20 4650.0 21 4769.0 22 5310.0 23 5995.0 24 7733.0 25 10497.0 26 14312.0 27 19102.0 28 24281.0 29 31056.0 30 38761.0 31 48704.0 32 61156.0 33 78153.0 34 102699.0 35 149091.0 36 278008.0 37 515709.0 38 219374.0 39 621.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70503682556723 19.03077862145787 12.725661993307034 24.538522559667868 2 15.8534428315693 20.719668371344323 38.535449222923404 24.891439574162973 3 18.83293784713855 25.380064409447513 29.901664603174417 25.885333140239524 4 12.817418007834657 16.594863814154063 36.761476527208956 33.826241650802324 5 12.706282478565258 39.30761856994893 34.491991464684986 13.49410748680083 6 32.343343624952965 37.03938473445366 16.846740334246167 13.77053130634721 7 28.386435442638263 33.34288951686666 20.92560837572777 17.3450666647673 8 27.327692528664 34.713739846373535 19.615743922662098 18.34282370230036 9 27.42206205122544 14.890620381680206 19.411692087085857 38.2756254800085 10-11 25.012434223255735 26.53092293393065 27.707485343048056 20.749157499765563 12-13 25.873244519148702 24.01113797773829 28.05543586255524 22.060181640557765 14-15 22.63764596116249 25.224172130009155 25.736735146890023 26.40144676193834 16-17 22.231767986296834 28.825170666391276 26.641317469757237 22.301743877554653 18-19 22.34608637900177 26.83404105550265 28.9080344988962 21.911838066599383 20-21 22.905619794096385 26.120682594342814 29.01361295200924 21.96008465955156 22-23 22.680676089374465 26.223776846348613 28.924496072684715 22.17105099159221 24-25 22.404571046006033 26.515758523229742 28.91772995597276 22.161940474791468 26-27 22.119615457093552 26.830488631827915 28.609006993173015 22.44088891790552 28-29 22.1657683708469 26.800315991208752 28.800129625244157 22.23378601270019 30-31 22.184253244344468 26.424647818358256 28.804259120221026 22.586839817076246 32-33 22.014480676801597 26.64128779380675 28.696288013408783 22.647943515982874 34-35 22.740858916970254 26.472906450720714 28.47478671894382 22.311447913365217 36-37 22.88042491212951 26.05343689053017 28.37006028966102 22.696077907679303 38-39 22.499296679973362 26.34052203558028 28.44060002397817 22.719581260468193 40-41 22.58683408799947 26.229055269575767 28.598048136007282 22.58606250641748 42-43 22.634776314778144 26.10065011471303 28.32077984089446 22.943793729614363 44-45 22.48312328695576 26.376340907822936 28.438374327691342 22.702161477529962 46-47 23.181932569118256 26.617873468869334 27.503759942927214 22.696434019085196 48-49 22.954288352901656 26.81388312186251 27.30404079612266 22.927787729113174 50-51 22.989935991923392 26.796679022746922 27.44408975078426 22.769295234545428 52-53 23.252739386989827 26.480592521897883 27.539044648061033 22.727623443051257 54-55 22.559360803767184 26.70221252016481 27.66745248583567 23.070974190232338 56-57 22.745081048228904 27.000120484716543 27.37754034309062 22.877258123963937 58-59 22.70786457501785 26.436900654594837 27.619314138453127 23.235920631934185 60-61 22.359718148354343 26.708333877395006 27.961762949756004 22.970185024494647 62-63 22.45934078717613 26.575325635750907 28.075214647169076 22.890118929903885 64-65 22.251917956680234 26.46976079996866 27.89717401858664 23.38114722476446 66-67 22.20183154085593 26.877575687792287 27.63888274761398 23.2817100237378 68-69 22.549686443907113 26.37818081675338 27.943379473501157 23.128753265838352 70-71 22.373500325841935 26.52611542995111 27.622671476544724 23.477712767662233 72-73 22.364686568546105 26.626657550214677 27.431795762986493 23.576860118252725 74-75 22.41997286431087 26.917125753620763 27.269943129008478 23.392958253059888 76-77 22.518823455396454 26.765155211156262 27.47218473160477 23.24383660184252 78-79 22.68987563402668 26.72874281990378 27.290864674104647 23.290516871964893 80-81 22.745785124751762 26.932616599777077 26.98855576645264 23.33304250901852 82-83 22.70664928446811 26.86626914729989 27.10655538973289 23.320526178499115 84-85 22.51490622993164 26.852728941055254 27.15132835489588 23.48103647411723 86-87 22.705425832083975 26.748417975079324 27.046720629415038 23.49943556342166 88-89 22.548914869194345 26.85347083981753 26.94979897511137 23.647815315876752 90-91 22.469308289914803 27.03971634446887 26.966713267953047 23.52426209766328 92-93 22.5669386769132 26.489423769172927 27.216278118755056 23.727359435158824 94-95 22.346225308401085 26.2774136359738 27.358304950650187 24.018056104974924 96-97 22.64089685864434 26.147367009058563 27.23508351663744 23.976652615659653 98-99 22.50530136537055 26.36229137698977 26.891136888747752 24.24127036889193 100-101 22.17839920192502 26.216739331817006 27.249357173874262 24.35550429238371 102-103 22.16520225501093 25.89140154657304 27.332406359023974 24.610989839392055 104-105 22.040280924802314 26.165536705558694 27.113546623694017 24.680635745944976 106-107 22.30144724165748 26.141496201294867 26.844430277333075 24.712626279714573 108-109 22.350130637343433 26.01011322694569 26.741789198845677 24.897966936865195 110-111 22.11554984156436 26.26262536329499 27.05808297771751 24.563741817423143 112-113 22.154145575931395 26.35637565408978 27.032990579207777 24.45648819077104 114-115 21.959769362785902 26.579250307378782 26.749951997568864 24.711028332266448 116-117 21.927774056742212 26.595713426383295 26.85062991675003 24.62588260012446 118-119 21.750559773746055 26.760256583369564 26.500290088183842 24.988893554700542 120-121 21.662510523899527 27.001811134545367 26.610086146853547 24.725592194701562 122-123 21.70194871831396 26.972385095180524 26.54712493864443 24.778541247861085 124-125 21.60121998391602 26.983564831238425 26.44873832182152 24.96647686302403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 76.0 1 63.0 2 114.5 3 179.5 4 132.0 5 100.5 6 250.5 7 619.5 8 1018.0 9 1158.5 10 930.0 11 654.0 12 587.0 13 645.0 14 710.0 15 790.0 16 891.0 17 1020.5 18 1177.5 19 1295.5 20 1495.5 21 1788.0 22 2253.0 23 2878.5 24 3557.0 25 4704.5 26 6398.0 27 8530.0 28 10994.5 29 14165.0 30 18072.0 31 22494.5 32 28816.5 33 32645.5 34 33815.5 35 35765.5 36 40032.0 37 45352.0 38 47588.5 39 49565.0 40 51252.5 41 53118.0 42 54525.0 43 55221.5 44 57636.0 45 61806.0 46 63253.0 47 62830.0 48 62725.5 49 61199.0 50 60644.5 51 59986.0 52 59248.0 53 60093.0 54 61516.5 55 58381.0 56 52139.5 57 47287.0 58 43426.0 59 38029.5 60 30783.0 61 25027.5 62 21110.0 63 16322.0 64 11752.0 65 8859.5 66 6360.5 67 5212.0 68 3907.5 69 2402.0 70 1678.0 71 1109.5 72 822.0 73 601.0 74 471.0 75 378.5 76 242.0 77 122.0 78 53.0 79 24.5 80 12.0 81 7.0 82 4.0 83 1.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04213984969724595 2 0.0053416710883832895 3 0.0011870380196407312 4 0.07840386119727029 5 0.0059945419991856925 6 0.0 7 0.0055197267913293995 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.9675950491018276E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.044911583473107E-4 28-29 5.34167108838329E-4 30-31 6.528709108024021E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.902785147305483E-4 42-43 5.638430593293473E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.9675950491018276E-5 52-53 0.0 54-55 0.0 56-57 2.967595049101828E-4 58-59 4.154633068742559E-4 60-61 4.748152078562924E-4 62-63 4.748152078562924E-4 64-65 0.0 66-67 2.9675950491018276E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.9675950491018276E-5 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.2643545540120104E-4 92-93 1.7805570294610965E-4 94-95 3.8578735638323755E-4 96-97 0.0030862988510659004 98-99 0.009407276305652794 100-101 0.018547469056886423 102-103 0.010297554820383343 104-105 0.004362364722179686 106-107 0.012641954909173785 108-109 0.014274132186179792 110-111 0.006677088860479112 112-113 0.0048668558805269975 114-115 0.0032940305045030286 116-117 2.9675950491018276E-5 118-119 0.0019882886828982245 120-121 6.231949603113838E-4 122-123 8.902785147305483E-4 124-125 0.03451313042105426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1684866.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.22939152896586 #Duplication Level Percentage of deduplicated Percentage of total 1 81.20192608062582 51.343483770580335 2 10.97206742169059 13.875142937765705 3 3.419093697462415 6.485616422132118 4 1.590160648099721 4.021795608506054 5 0.7901607039883506 2.498069026164136 6 0.4699713088556479 1.7829599937008584 7 0.29749352142833163 1.3167234040605942 8 0.21596405621411452 1.0924220693236673 9 0.13886087244097714 0.7902079624461896 >10 0.8030647287784548 9.390370030052871 >50 0.07177636100584568 3.13295159650311 >100 0.028471988696298852 3.1953480492101636 >500 3.954442853791506E-4 0.16977513351180556 >1k 5.931664280687259E-4 0.9051339960423214 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2935 0.1741978293822773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2640 0.1566890185925765 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.935190098203655E-5 0.0 14-15 0.0 0.0 0.0 5.935190098203655E-5 0.0 16-17 0.0 0.0 0.0 5.935190098203655E-5 0.0 18-19 5.935190098203655E-5 0.0 0.0 5.935190098203655E-5 0.0 20-21 5.935190098203655E-5 0.0 0.0 8.902785147305482E-5 0.0 22-23 5.935190098203655E-5 0.0 0.0 2.670835544191645E-4 0.0 24-25 5.935190098203655E-5 0.0 0.0 7.715747127664751E-4 0.0 26-27 5.935190098203655E-5 0.0 0.0 0.0013057418216048042 0.0 28-29 5.935190098203655E-5 0.0 0.0 0.0039172254648144125 0.0 30-31 5.935190098203655E-5 0.0 0.0 0.01563922590876663 0.0 32-33 5.935190098203655E-5 0.0 0.0 0.039558042004527366 0.0 34-35 5.935190098203655E-5 0.0 0.0 0.07519885854424033 0.0 36-37 5.935190098203655E-5 0.0 0.0 0.13176122018012115 0.0 38-39 5.935190098203655E-5 0.0 0.0 0.2206703678512119 0.0 40-41 5.935190098203655E-5 0.0 0.0 0.3160191967788536 0.0 42-43 5.935190098203655E-5 0.0 0.0 0.40837075470690254 0.0 44-45 5.935190098203655E-5 0.0 0.0 0.5215845058301372 0.0 46-47 5.935190098203655E-5 0.0 0.0 0.659162212306498 0.0 48-49 5.935190098203655E-5 0.0 0.0 0.8054646482272181 0.0 50-51 5.935190098203655E-5 0.0 0.0 0.9727183051945971 0.0 52-53 5.935190098203655E-5 0.0 0.0 1.127122275599365 0.0 54-55 5.935190098203655E-5 0.0 0.0 1.2832177751821212 0.0 56-57 5.935190098203655E-5 0.0 0.0 1.4682176505431292 0.0 58-59 5.935190098203655E-5 0.0 0.0 1.6744061545547242 0.0 60-61 5.935190098203655E-5 0.0 0.0 1.9131195003044752 0.0 62-63 5.935190098203655E-5 0.0 0.0 2.143464228015759 0.0 64-65 5.935190098203655E-5 0.0 0.0 2.3733044645686956 0.0 66-67 5.935190098203655E-5 0.0 0.0 2.6343934769886745 0.0 68-69 5.935190098203655E-5 0.0 0.0 2.942162759531025 0.0 70-71 5.935190098203655E-5 0.0 0.0 3.2723373846940946 0.0 72-73 5.935190098203655E-5 0.0 0.0 3.6089220151632233 0.0 74-75 5.935190098203655E-5 0.0 0.0 3.937375435197814 0.0 76-77 5.935190098203655E-5 0.0 0.0 4.291735960011064 0.0 78-79 5.935190098203655E-5 0.0 0.0 4.7006408818268035 0.0 80-81 5.935190098203655E-5 0.0 0.0 5.149311577300509 0.0 82-83 5.935190098203655E-5 0.0 0.0 5.618369650761544 0.0 84-85 5.935190098203655E-5 0.0 0.0 6.079622949243442 0.0 86-87 5.935190098203655E-5 0.0 0.0 6.543576759220022 0.0 88-89 5.935190098203655E-5 0.0 0.0 7.044951942765775 0.0 90-91 5.935190098203655E-5 0.0 0.0 7.609566576807889 0.0 92-93 5.935190098203655E-5 0.0 0.0 8.19628979396581 0.0 94-95 5.935190098203655E-5 0.0 0.0 8.772181289194512 0.0 96-97 5.935190098203655E-5 0.0 0.0 9.344363290611835 0.0 98-99 5.935190098203655E-5 0.0 0.0 9.95218610856887 0.0 100-101 5.935190098203655E-5 0.0 0.0 10.623010969418338 0.0 102-103 5.935190098203655E-5 0.0 0.0 11.348350551319808 0.0 104-105 5.935190098203655E-5 0.0 0.0 12.061493317569468 0.0 106-107 5.935190098203655E-5 0.0 0.0 12.75804722749465 0.0 108-109 5.935190098203655E-5 0.0 0.0 13.484722227168215 0.0 110-111 5.935190098203655E-5 0.0 0.0 14.263864307309898 0.0 112-113 5.935190098203655E-5 0.0 0.0 15.07695567481331 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2975 0.0 71.29255 1 GTATCAA 4930 0.0 49.06369 1 ATCAACG 6385 0.0 37.765163 3 TCAACGC 6470 0.0 37.267914 4 GTACATG 8685 0.0 37.1115 1 CAACGCA 6670 0.0 36.12898 5 TATCAAC 6790 0.0 35.342484 2 AACGCAG 6885 0.0 35.08719 6 GTGGTAT 1360 0.0 35.045494 1 TGGTATC 1335 0.0 34.791656 2 TACATGG 9115 0.0 34.558968 2 ACATGGG 9090 0.0 33.731884 3 CATGGGA 6545 0.0 30.10944 4 ACGCAGA 8415 0.0 28.636992 7 CGCAGAG 8610 0.0 27.916826 8 TAAGGTG 1165 0.0 27.580004 5 CATGGGG 3355 0.0 26.441013 4 GAGTACT 5200 0.0 25.056974 12-13 GAGTACA 6635 0.0 24.60315 1 ATGGGAT 2850 0.0 24.0093 5 >>END_MODULE