FastQCFastQC Report
Thu 26 May 2016
SRR1364433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396324
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.047.7788121
GTATCAA25350.043.6794471
ATCAACG26250.040.3288423
CAACGCA28000.037.6021655
GTACATG72300.035.9821
AACGCAG30200.034.6652456
TACATGG73650.034.6432572
ACATGGG74050.033.4916153
TATCAAC32550.032.889382
TCAACGC32900.031.9971684
ACGCAGA34900.029.6559837
CATGGGG47000.029.3578534
CGCAGAG37350.027.8699368
TAAGGTA9900.026.4333154
TAAGGTG16150.023.2041475
GTGTAAG11350.023.0780411
CATGGGA31800.022.2563534
GTAAGGT16000.021.5596734
GAGTACA58650.021.3153951
AGAGTAC39850.020.82344410-11
Produced by FastQC (version 0.11.5)