FastQCFastQC Report
Thu 26 May 2016
SRR1364433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396324
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.066.832191
GTATCAA46250.053.0768281
ATCAACG57150.042.4111333
CAACGCA57450.042.151815
AACGCAG60550.040.0903436
TATCAAC63100.039.0750852
TCAACGC63000.038.5674864
ACGCAGA71450.033.8911177
CGCAGAG72200.033.5383648
GTACATG73550.033.3759771
TACATGG75350.032.090152
ACATGGG73500.031.6804333
TAAGGTG18000.030.7413045
AGGTAAG16900.028.192412
GTAAGGT17350.028.1459624
GGTAAGG17800.027.0998423
CATGGGG48250.026.042754
TAAGGTA9350.024.840184
AGAGTAC76700.024.58943610-11
GCAGAGT97100.024.3251749
Produced by FastQC (version 0.11.5)