FastQCFastQC Report
Thu 26 May 2016
SRR1364432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919624
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.057.9152831
GTATCAA26800.047.752491
ATCAACG27250.043.2099383
CAACGCA27600.043.0996485
TCAACGC28550.041.6589974
GTACATG79450.039.7076641
AACGCAG30300.039.0627946
TACATGG85000.036.7303732
TATCAAC34300.036.0623632
ACATGGG83700.035.879873
CATGGGA47950.032.6176034
ACGCAGA37100.032.063357
CGCAGAG38550.030.5487618
CATGGGG39350.029.1673874
GTATACG1608.717951E-626.0417651
GAGTACA54650.025.3780441
ATGGGAG24800.024.9421845
GTGTAAG11050.024.779241
GCAGAGT47950.023.5683489
ATGGGAT23500.023.537915
Produced by FastQC (version 0.11.5)