##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1919624 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.727713343863172 31.0 30.0 34.0 25.0 34.0 2 29.777699695357008 31.0 30.0 34.0 25.0 34.0 3 29.682052839514405 31.0 30.0 34.0 25.0 34.0 4 33.20632113372202 35.0 35.0 37.0 27.0 37.0 5 33.378620500681386 37.0 35.0 37.0 27.0 37.0 6 33.42034742220351 37.0 35.0 37.0 28.0 37.0 7 33.30292546873763 37.0 35.0 37.0 27.0 37.0 8 33.156334261292834 37.0 35.0 37.0 26.0 37.0 9 34.10043112609553 38.0 34.0 39.0 24.0 39.0 10-11 34.29245128212608 38.0 34.0 39.0 26.0 39.0 12-13 34.33807584193571 38.0 34.5 39.0 25.5 39.0 14-15 35.3545876692519 39.0 35.0 41.0 25.5 41.0 16-17 35.47801470496306 39.0 35.0 41.0 26.5 41.0 18-19 35.42923900722225 39.0 35.0 41.0 26.0 41.0 20-21 35.364641721503794 39.0 34.5 41.0 25.0 41.0 22-23 35.253845805220195 39.0 34.0 41.0 25.0 41.0 24-25 35.29112914820819 39.0 34.0 41.0 25.0 41.0 26-27 35.2798412605802 39.0 34.0 41.0 25.0 41.0 28-29 35.210836080399076 39.0 34.0 41.0 25.0 41.0 30-31 34.98698156513984 39.0 34.0 41.0 24.0 41.0 32-33 35.03860495597054 39.0 34.0 41.0 25.0 41.0 34-35 34.97730623288727 39.0 34.0 41.0 25.0 41.0 36-37 34.84576823377911 39.0 34.0 41.0 24.0 41.0 38-39 34.69098297374903 39.0 33.5 41.0 24.0 41.0 40-41 34.64570561735006 39.0 33.0 41.0 24.0 41.0 42-43 34.486210580822075 38.5 33.0 41.0 23.0 41.0 44-45 34.45527144899209 38.0 33.0 40.0 23.0 41.0 46-47 34.29763432838931 38.0 33.0 40.0 23.0 41.0 48-49 34.11529054648202 38.0 33.0 40.0 22.0 41.0 50-51 33.95837752601551 38.0 33.0 40.0 21.0 41.0 52-53 33.804611736465056 38.0 33.0 40.0 21.0 41.0 54-55 33.659026194713135 38.0 33.0 40.0 20.5 41.0 56-57 33.60225466028764 38.0 32.5 40.0 20.5 41.0 58-59 33.569485222105996 37.5 33.0 40.0 20.5 41.0 60-61 33.37243465386972 37.0 32.0 40.0 20.0 41.0 62-63 33.09887014331973 37.0 32.0 40.0 18.5 41.0 64-65 32.78855312290324 36.0 32.0 39.5 18.0 41.0 66-67 32.47787795943372 36.0 32.0 39.0 16.5 41.0 68-69 32.17721178730835 35.0 31.0 39.0 13.5 41.0 70-71 31.822632453022052 35.0 31.0 38.5 12.5 40.0 72-73 31.50272501281501 35.0 31.0 37.5 9.5 40.0 74-75 31.157015384262753 35.0 31.0 37.0 7.5 39.0 76-77 30.82180546815418 35.0 31.0 36.5 7.0 39.0 78-79 30.517551353806788 35.0 30.0 36.0 4.0 39.0 80-81 30.218666259642514 35.0 30.0 36.0 2.0 37.0 82-83 29.940430261342847 34.0 30.0 35.0 2.0 37.0 84-85 29.625894185528 34.0 30.0 35.0 2.0 36.5 86-87 29.44533043971111 34.0 30.0 35.0 2.0 36.0 88-89 29.304415343838166 34.0 29.5 35.0 2.0 36.0 90-91 29.1805293640838 34.0 29.0 35.0 2.0 36.0 92-93 28.960554254374816 34.0 29.0 35.0 2.0 35.0 94-95 28.883271151017077 34.0 29.0 35.0 2.0 35.0 96-97 28.811678484953305 34.0 29.0 35.0 2.0 35.0 98-99 28.661729588711125 34.0 29.0 35.0 2.0 35.0 100-101 28.53657643371827 34.0 29.0 35.0 2.0 35.0 102-103 27.508611582268195 32.5 26.0 34.5 2.0 35.0 104-105 28.463389965951666 34.0 29.0 35.0 2.0 35.0 106-107 28.7222627972978 34.0 29.0 35.0 2.0 35.0 108-109 28.7260867232333 34.0 29.0 35.0 2.0 35.0 110-111 28.619213450133984 34.0 29.0 35.0 2.0 35.0 112-113 28.503510843790245 34.0 29.0 35.0 2.0 35.0 114-115 28.402015186307317 34.0 29.0 35.0 2.0 35.0 116-117 28.27730222168508 34.0 29.0 35.0 2.0 35.0 118-119 28.016535529874602 34.0 29.0 35.0 2.0 35.0 120-121 27.878961973803204 34.0 29.0 35.0 2.0 35.0 122-123 27.637636328781053 34.0 29.0 35.0 2.0 35.0 124-125 26.456770440461256 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0690766851408497 1101 2 1.0703579894108444 1101 3 1.2522401478952716 1101 4 1.2254205433877132 1101 5 0.9849223583695021 1101 6 1.0294115532637207 1101 7 1.086500104170831 1101 8 1.2058770818758688 1101 9 1.68383794496296 1101 10-11 1.4253326692488812 1101 12-13 1.194927131157435 1101 14-15 1.454576096376904 1101 16-17 1.18816150460718 1101 18-19 1.3393427273837233 1101 20-21 1.315133209357505 1101 22-23 1.3423356429248798 1101 24-25 1.2512327063518498 1101 26-27 1.2957247151285856 1101 28-29 1.2825567142194245 1101 30-31 1.404591580876101 1101 32-33 1.2860468636456943 1101 34-35 1.1711091613712838 1101 36-37 1.0959998874029324 1101 38-39 1.2344312403960842 1101 40-41 1.241225840034467 1101 42-43 1.2200371062117554 1101 44-45 1.1045843879287887 1101 46-47 1.1773538088051936 1101 48-49 1.0861256314544363 1101 50-51 1.0959928864874584 1101 52-53 1.0041401831116517 1101 54-55 0.8901319981339455 1101 56-57 0.9646957341008573 1101 58-59 0.9416255198808656 1101 60-61 0.9287119232959569 1101 62-63 1.0174296594911496 1101 64-65 0.9208610811234905 1101 66-67 1.0554527810188006 1101 68-69 0.9496859528809622 1101 70-71 0.8980765453443702 1101 72-73 0.9692130167550879 1101 74-75 0.8957865215072225 1101 76-77 1.054677438281871 1101 78-79 0.9154113879018801 1101 80-81 0.9616692158029885 1101 82-83 1.0128558604185933 1101 84-85 1.1598515153369853 1101 86-87 1.1223441122674522 1101 88-89 0.9818683236824697 1101 90-91 0.9686333796342126 1101 92-93 1.119315777844978 1101 94-95 1.037859105540221 1101 96-97 1.024177423198065 1101 98-99 1.0578305375749082 1101 100-101 1.0434977311392224 1101 102-103 1.0644036483447437 1101 104-105 1.0254088342363836 1101 106-107 0.9640040452007739 1101 108-109 1.0237037987711481 1101 110-111 1.117345291153672 1101 112-113 0.9609242245858738 1101 114-115 1.0716594646139121 1101 116-117 1.0330896906708702 1101 118-119 0.913650004378578 1101 120-121 0.7690223785101722 1101 122-123 0.9709320116619118 1101 124-125 0.5513017330287013 1102 1 0.6520683221116883 1102 2 0.5087735848510597 1102 3 0.6180556248631284 1102 4 0.5303025141334388 1102 5 0.5078462935175452 1102 6 0.4137705198489172 1102 7 0.623398880267402 1102 8 0.7169289438509097 1102 9 1.1161827648289986 1102 10-11 0.7439983043353493 1102 12-13 0.7032343057315842 1102 14-15 0.9422180649770695 1102 16-17 0.7100482818791392 1102 18-19 0.6538437558544103 1102 20-21 0.8221640658100497 1102 22-23 0.8752209929314034 1102 24-25 0.7483031974057823 1102 26-27 0.8435852745606596 1102 28-29 0.9057160782000935 1102 30-31 0.8516621302333647 1102 32-33 0.7560063078517487 1102 34-35 0.6826244764890959 1102 36-37 0.7273259149407565 1102 38-39 0.8144473087928787 1102 40-41 0.7093408585049588 1102 42-43 0.783746433811153 1102 44-45 0.6765786182315949 1102 46-47 0.742596688802962 1102 48-49 0.6517938925227469 1102 50-51 0.5386411285255548 1102 52-53 0.6156648850650512 1102 54-55 0.3968351898912488 1102 56-57 0.5274583905869861 1102 58-59 0.6297276766375219 1102 60-61 0.5294524921498578 1102 62-63 0.5920057360037703 1102 64-65 0.6651584910565091 1102 66-67 0.6482278910954804 1102 68-69 0.6136881963929639 1102 70-71 0.6922771134519827 1102 72-73 0.6693531196488003 1102 74-75 0.7451267610639327 1102 76-77 0.8551320876223407 1102 78-79 0.6910465443413685 1102 80-81 0.8099250134857812 1102 82-83 0.737287869411297 1102 84-85 0.8600866526529813 1102 86-87 0.7631028600985701 1102 88-89 0.9052807241003862 1102 90-91 0.7450642598745141 1102 92-93 0.8155616266481296 1102 94-95 0.7619375631606964 1102 96-97 0.7260084391685098 1102 98-99 0.7657941889490019 1102 100-101 0.6778438126892503 1102 102-103 0.6795555168497529 1102 104-105 0.7017706833380437 1102 106-107 0.5931529682217267 1102 108-109 0.7510204231484714 1102 110-111 0.782839575095533 1102 112-113 0.7324279490023713 1102 114-115 0.5853687339099807 1102 116-117 0.5493137573512215 1102 118-119 0.64962123285299 1102 120-121 0.5931824607878227 1102 122-123 0.6653997839151273 1102 124-125 0.39822990566251804 1103 1 0.6111610943083754 1103 2 0.6800717225142456 1103 3 0.816270517978527 1103 4 0.7674392207775966 1103 5 0.6765472290052514 1103 6 0.6200937679241321 1103 7 0.7135086353992648 1103 8 0.840793891137487 1103 9 1.3095243888126191 1103 10-11 1.042226459041764 1103 12-13 0.8706154935741992 1103 14-15 1.0608208376956725 1103 16-17 0.9506921113137139 1103 18-19 1.0346864484140639 1103 20-21 1.018403949207034 1103 22-23 1.0711139828026575 1103 24-25 1.0225487377318174 1103 26-27 1.1598727017175179 1103 28-29 1.0265926120003854 1103 30-31 1.2941696462597605 1103 32-33 1.1499724076706883 1103 34-35 1.0560308921746326 1103 36-37 1.0240387725875308 1103 38-39 1.0982240974316895 1103 40-41 1.066347669046202 1103 42-43 1.13214215248815 1103 44-45 0.954931314912578 1103 46-47 0.9550972624595744 1103 48-49 0.8308883047024409 1103 50-51 0.8892305213224105 1103 52-53 0.8312146685602997 1103 54-55 0.8734804482931793 1103 56-57 0.8910711381816796 1103 58-59 0.9962625297634347 1103 60-61 0.904433707707966 1103 62-63 1.041386445574254 1103 64-65 1.015099651885528 1103 66-67 0.8402010066222729 1103 68-69 0.861674661696405 1103 70-71 0.9811205272442116 1103 72-73 0.9405649726091418 1103 74-75 0.9406444557832323 1103 76-77 0.8424640624768358 1103 78-79 0.8389177834036481 1103 80-81 0.8913255364806361 1103 82-83 0.8354995680467638 1103 84-85 0.896353617141159 1103 86-87 0.9829574865153887 1103 88-89 0.9690145357358517 1103 90-91 0.9072242727244202 1103 92-93 0.9443062545227114 1103 94-95 0.8302066364555643 1103 96-97 0.897629792962114 1103 98-99 0.854118972174291 1103 100-101 0.8384604080575251 1103 102-103 0.7888296770164764 1103 104-105 0.8160062982799339 1103 106-107 0.8331062725737759 1103 108-109 0.9123809161602061 1103 110-111 0.9178218187362717 1103 112-113 0.9251537919851351 1103 114-115 0.9237075211054275 1103 116-117 0.7622889474902657 1103 118-119 0.5672147298816732 1103 120-121 0.3384369708535857 1103 122-123 0.46826859562257894 1103 124-125 0.23044968903178642 1104 1 0.725110256592302 1104 2 0.7251563806499242 1104 3 0.8308776718792217 1104 4 0.6863804756969216 1104 5 0.825212076725883 1104 6 0.6986641598783692 1104 7 0.8627963971941952 1104 8 0.9141674364903452 1104 9 1.3872599309939986 1104 10-11 1.0773396437863951 1104 12-13 1.0019392808150585 1104 14-15 1.1919902816185939 1104 16-17 1.0146069206216595 1104 18-19 1.1215169516404018 1104 20-21 1.1889465493479818 1104 22-23 1.198289626883664 1104 24-25 1.2496748129405404 1104 26-27 1.2647343617291824 1104 28-29 1.2441547824691526 1104 30-31 1.2576144143675805 1104 32-33 1.2474551038507968 1104 34-35 1.3021376792102615 1104 36-37 1.069365361343408 1104 38-39 1.1767689697279096 1104 40-41 1.1187620631136213 1104 42-43 1.0996749140816888 1104 44-45 0.9212925696997729 1104 46-47 1.0855884908363223 1104 48-49 1.1730210896099749 1104 50-51 1.0892015493181617 1104 52-53 0.8993410738729963 1104 54-55 0.9187637228904535 1104 56-57 1.0068561175347241 1104 58-59 1.0619780466318716 1104 60-61 1.1054928108097286 1104 62-63 1.0565076154678295 1104 64-65 1.0168141040674428 1104 66-67 0.9254643684275194 1104 68-69 0.869506000092187 1104 70-71 0.8685586434054393 1104 72-73 0.8085475195072327 1104 74-75 0.7828898681585308 1104 76-77 0.9046588255233488 1104 78-79 0.6865880677171496 1104 80-81 0.812834473901745 1104 82-83 0.786958940857744 1104 84-85 0.8616719756741347 1104 86-87 0.8580527723366238 1104 88-89 0.8643871877408458 1104 90-91 0.7553912580227546 1104 92-93 0.8204419325816232 1104 94-95 0.832449862344923 1104 96-97 0.7063365142326816 1104 98-99 0.7968328274412819 1104 100-101 0.735185228999029 1104 102-103 0.7447326136631531 1104 104-105 0.6570310825029644 1104 106-107 0.6711072703444536 1104 108-109 0.620285734122465 1104 110-111 0.6519749635359808 1104 112-113 0.6540953857458511 1104 114-115 0.6993840797385182 1104 116-117 0.6971633662654035 1104 118-119 0.7069642131485594 1104 120-121 0.630957409489092 1104 122-123 0.7230767868893828 1104 124-125 0.636341714559542 1105 1 0.4214797459958639 1105 2 0.4845268008293928 1105 3 0.5778152076973555 1105 4 0.28507928357252865 1105 5 0.3809258206255137 1105 6 0.4999981251819676 1105 7 0.5697786261139726 1105 8 0.6618520598344517 1105 9 1.2350177213416842 1105 10-11 0.6926785488538627 1105 12-13 0.7453496252461917 1105 14-15 0.4005494995377674 1105 16-17 0.5057677431051246 1105 18-19 0.5610303932691281 1105 20-21 0.8440794282911739 1105 22-23 0.9072465059255279 1105 24-25 1.021962446924043 1105 26-27 0.8488234457496588 1105 28-29 0.7913454074016926 1105 30-31 0.9971745709916604 1105 32-33 0.9335182212937525 1105 34-35 0.8832837845980492 1105 36-37 0.8918925390749166 1105 38-39 0.95470204812937 1105 40-41 0.943680053078154 1105 42-43 0.9421838198847894 1105 44-45 0.7814803811606055 1105 46-47 0.8452321309674318 1105 48-49 0.6130174290573009 1105 50-51 0.6905187976573757 1105 52-53 0.6088585989341269 1105 54-55 0.5923059661528995 1105 56-57 0.6273628551982071 1105 58-59 0.6400233643621149 1105 60-61 0.52517374364718 1105 62-63 0.6535625885114555 1105 64-65 0.6090440634225374 1105 66-67 0.5492983106048825 1105 68-69 0.4821927664674348 1105 70-71 0.5281271342842295 1105 72-73 0.4737700445485409 1105 74-75 0.5461885547205121 1105 76-77 0.47220769566359166 1105 78-79 0.3590179390603936 1105 80-81 0.3354347525618735 1105 82-83 0.37919616845822546 1105 84-85 0.570411798478716 1105 86-87 0.550279471993786 1105 88-89 0.5296638942477756 1105 90-91 0.4833314725196871 1105 92-93 0.4690448436680761 1105 94-95 0.5294252591140349 1105 96-97 0.4948429074253724 1105 98-99 0.43878485349772234 1105 100-101 0.24311466446780372 1105 102-103 0.2883844840910399 1105 104-105 0.37879108592124666 1105 106-107 0.32050847704939045 1105 108-109 0.37237315217300093 1105 110-111 0.40529820355238755 1105 112-113 0.40494907420418613 1105 114-115 0.2896915881884041 1105 116-117 0.2163932714390313 1105 118-119 0.04458357205407637 1105 120-121 0.10104175197057685 1105 122-123 0.1817441474899404 1105 124-125 0.0026230687790231855 1106 1 0.6563092827639387 1106 2 0.6577943221782476 1106 3 0.784141675908856 1106 4 0.6131964347289696 1106 5 0.5658221885902179 1106 6 0.5678156279666027 1106 7 0.550540851199699 1106 8 0.7669976251695871 1106 9 1.2233965188169478 1106 10-11 0.6424888661928492 1106 12-13 0.874477216047076 1106 14-15 1.1132263510458387 1106 16-17 0.9395367168312845 1106 18-19 0.991354611933815 1106 20-21 1.0407104589330558 1106 22-23 0.9483118738247072 1106 24-25 0.9299254058425035 1106 26-27 1.094553238048789 1106 28-29 1.0044412643043117 1106 30-31 0.9829956555789892 1106 32-33 1.0507497498935692 1106 34-35 1.045370621473154 1106 36-37 0.9649557447883268 1106 38-39 1.1551443134011734 1106 40-41 1.0518964682178265 1106 42-43 1.086930145260986 1106 44-45 0.946443471120638 1106 46-47 0.9702909904690387 1106 48-49 0.9113208776344095 1106 50-51 0.8719096946368694 1106 52-53 0.9524169233493467 1106 54-55 0.8343934582358088 1106 56-57 0.8304088071059539 1106 58-59 0.9323216149572673 1106 60-61 0.9090514818698168 1106 62-63 1.0037624268935232 1106 64-65 1.0396726261149993 1106 66-67 0.9026768896775437 1106 68-69 0.9352377355634687 1106 70-71 0.9691970886380865 1106 72-73 0.86548636140704 1106 74-75 0.8421872006243696 1106 76-77 0.8967232786893398 1106 78-79 0.8477458530971269 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-2.2488730313507332 2216 114-115 -2.381758371862915 2216 116-117 -2.2169049652653463 2216 118-119 -1.9705542386137331 2216 120-121 -1.927760624646389 2216 122-123 -1.9592876649866007 2216 124-125 -1.9223022841526252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61538.0 3 11356.0 4 7088.0 5 6138.0 6 6134.0 7 7200.0 8 8038.0 9 8676.0 10 9382.0 11 9946.0 12 10454.0 13 10812.0 14 10747.0 15 10120.0 16 9493.0 17 8693.0 18 8232.0 19 7936.0 20 8265.0 21 8666.0 22 9935.0 23 12694.0 24 17826.0 25 23771.0 26 27776.0 27 31547.0 28 36984.0 29 43387.0 30 51153.0 31 60825.0 32 74370.0 33 94816.0 34 125545.0 35 181357.0 36 310614.0 37 447347.0 38 140554.0 39 209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5944114061788 18.22471373498549 12.39169583801274 24.78917902082297 2 15.855767061867029 19.869432287794908 39.03195272807024 25.242847922267824 3 18.736429634136684 24.595910449129622 29.093822540247466 27.573837376486228 4 12.549333811216643 15.997907871321585 36.765306505186565 34.687451812275214 5 13.321136322434512 38.65672776980156 34.48835067378761 13.533785233976317 6 33.0187219638084 36.52811993225732 17.104141086477146 13.349017017457129 7 29.308968839731115 32.406242055735916 20.767035627810447 17.517753476722525 8 27.053226165229127 35.01503697903887 19.824484358989185 18.107252496742813 9 26.889051653092753 15.919658848080918 19.278667482108382 37.91262201671795 10-11 25.021106399960612 26.66090631050751 27.65473706804818 20.663250221483697 12-13 25.55719798445756 23.956928134112495 27.535492743864875 22.95038113756507 14-15 22.703373753639223 25.562576629747202 24.47791487380951 27.25613474280407 16-17 22.97647811441497 28.7591673985999 25.612219169461557 22.652135317523577 18-19 23.254090981085206 27.578094390583452 26.658952225343658 22.50886240298769 20-21 23.459285293551407 26.911130233029496 26.831255843712963 22.798328629706134 22-23 23.49865493256949 26.70212181228657 26.750417639073877 23.04880561607006 24-25 23.250611259045147 27.05719342356644 26.58418077742043 23.108014539967982 26-27 22.878707480175585 27.364166189307305 26.404185445189377 23.35294088532773 28-29 23.173774984175278 27.342957542856993 26.46680194741764 23.01646552555009 30-31 23.05386717770002 27.03829128953338 26.400854292708836 23.50698724005776 32-33 22.78588763106067 27.23144690357805 26.362844759930372 23.619820705430904 34-35 23.584450819038032 27.097839940049994 26.157357302916573 23.1603519379954 36-37 23.6135725485906 26.626658540015942 26.20498434577857 23.55478456561489 38-39 23.134985555708653 26.995574376444466 26.424444674235716 23.44499539361117 40-41 23.42530421813374 26.67927524219978 26.536224085001354 23.359196454665128 42-43 23.0686459677224 26.619734195194596 26.42784191118151 23.8837779259015 44-45 23.11687723092888 26.918260224504635 26.62892971675408 23.335932827812403 46-47 23.699062876218292 27.175104982972986 25.924165332619292 23.20166680818943 48-49 23.218857769371493 27.054538937572918 26.07762533645469 23.648977956600895 50-51 23.313003608712798 27.066492109229333 26.326099063255885 23.294405218801984 52-53 23.558577344277694 26.779915148297718 26.2482309537572 23.413276553667387 54-55 22.994203023888627 27.01559145358903 26.365170421303198 23.62503510121915 56-57 23.107537340765454 27.344006018494156 26.330625393022988 23.217831247717406 58-59 23.08635488738805 26.63490575793895 26.398144672510888 23.880594682162116 60-61 22.77616327534323 26.974072213932633 26.66942098813801 23.58034352258613 62-63 22.956279583505918 26.8571473229288 26.93170361918457 23.254869474380715 64-65 22.828162107768808 26.89453210982408 26.478371555624094 23.798934226783018 66-67 22.767824928732587 27.1768049222773 26.31497916878869 23.740390980201425 68-69 22.99919868956046 26.64800068356261 26.853017366241595 23.499783260635336 70-71 22.968847883534142 26.540055977171523 26.628619208062382 23.862476931231953 72-73 22.80945714519424 26.789652554793204 26.434402634637816 23.96648766537474 74-75 22.844572472965 27.142897334742134 26.384052217296976 23.628477974995885 76-77 23.11771463103961 26.706433656418415 26.619198078681332 23.556653633860634 78-79 23.04217581461501 26.642996876733882 26.66977517172754 23.64505213692357 80-81 23.186009138044174 26.815592976434328 26.49300787745361 23.505390008067884 82-83 23.037124550450493 26.794596418192736 26.571309851467934 23.596969179888838 84-85 22.935509274346806 26.860242263866454 26.694118618586938 23.510129843199806 86-87 23.163807808896795 26.83311815049044 26.58146363930979 23.421610401302974 88-89 22.917981162953428 26.831433077528665 26.531688847461815 23.718896912056096 90-91 22.806533561323384 26.96935784538982 26.6165359475081 23.607572645778692 92-93 22.996082190638937 26.48027549701996 26.863798816321427 23.659843496019672 94-95 22.730075356994288 26.359422828305927 26.99147444064448 23.9190273740553 96-97 23.03262315870199 26.09699005019188 27.019501582237265 23.850885208868863 98-99 22.800433656404923 26.393936520704436 26.76861898877411 24.037010834116526 100-101 22.605286335120418 26.24370220074236 27.12826605738555 24.02274540675167 102-103 22.52488485276967 25.938390337974475 27.275299111879526 24.26142569737633 104-105 22.234989458997674 26.304053408092436 27.030969681039924 24.429987451869962 106-107 22.500987880522853 26.211693744274267 26.8092930838986 24.47802529130428 108-109 22.60246301428167 26.119026849142656 26.735835349638993 24.542674786936683 110-111 22.587743004823952 26.259277560864682 26.974079224958174 24.178900209353195 112-113 22.41267658099086 26.42302853168494 27.268272860123627 23.89602202720057 114-115 22.407542339170288 26.62081437402164 26.90049217144437 24.071151115363705 116-117 22.269363763723714 26.574257436059344 27.109712060610704 24.04666673960624 118-119 22.09137626155177 26.689634901135484 26.877245941968226 24.34174289534452 120-121 22.113348906597412 26.709302949662366 27.01499295764006 24.16235518610016 122-123 22.14614685761615 26.73046124008084 27.06168826516296 24.061703637140052 124-125 21.99098049341761 26.877586554862308 26.988940099625523 24.14249285209456 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 595.0 1 486.0 2 322.5 3 241.5 4 193.5 5 162.0 6 153.0 7 151.0 8 141.0 9 144.0 10 148.5 11 130.0 12 131.5 13 154.0 14 176.5 15 209.0 16 245.0 17 286.0 18 337.0 19 407.0 20 551.0 21 797.0 22 1128.0 23 1576.5 24 2222.0 25 3326.5 26 5004.0 27 7403.0 28 10192.5 29 13412.0 30 17246.5 31 21848.0 32 28553.0 33 32382.5 34 33799.5 35 37713.5 36 44182.0 37 50486.0 38 53737.0 39 56092.0 40 57593.0 41 59558.5 42 61375.5 43 63412.0 44 66266.5 45 69951.0 46 71274.0 47 70469.5 48 69859.5 49 69152.0 50 69568.5 51 69320.5 52 70254.0 53 73363.0 54 76876.0 55 73367.5 56 65062.0 57 59792.5 58 56460.0 59 49537.5 60 39068.5 61 31661.0 62 26397.0 63 19907.5 64 14070.0 65 10364.5 66 7607.5 67 5852.5 68 4402.0 69 3184.5 70 2371.5 71 1668.5 72 1221.0 73 954.0 74 734.5 75 510.5 76 352.0 77 232.5 78 134.0 79 66.0 80 45.5 81 32.5 82 20.5 83 17.5 84 14.0 85 8.0 86 5.5 87 7.5 88 7.0 89 4.5 90 3.0 91 3.5 92 3.0 93 1.0 94 0.5 95 2.0 96 2.0 97 1.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08574595858355595 2 3.6465474488754044E-4 3 0.0 4 0.0025004896792288492 5 0.0014586189795501618 6 3.6465474488754044E-4 7 0.0 8 0.0011981513046304903 9 5.209353498393435E-4 10-11 0.00950707013456802 12-13 0.0021618817018332756 14-15 0.004453997241126387 16-17 0.002604676749196718 18-19 0.01458618979550162 20-21 0.018779719361708334 22-23 0.010601034369230641 24-25 0.01172104537138523 26-27 0.010965689114118181 28-29 0.008282872062445561 30-31 0.01956112238646735 32-33 0.002370255841769013 34-35 0.0012762916071063916 36-37 7.293094897750809E-4 38-39 3.9070151237950766E-4 40-41 0.0016148995845019648 42-43 0.0029953782615762255 44-45 0.001041870699678687 46-47 0.0014065254445662276 48-49 0.008361012364921464 50-51 0.005365634103345238 52-53 0.00820473175996966 54-55 0.009116368622188512 56-57 0.010783361741674411 58-59 0.009298695994632282 60-61 0.011252203556529821 62-63 0.014013160910678342 64-65 0.008986134784728676 66-67 0.013987114143186374 68-69 0.013961067375694407 70-71 0.0047665584510299935 72-73 0.01411734798064621 74-75 0.013544319095822932 76-77 0.005678195313248845 78-79 0.008282872062445561 80-81 0.014872704237913259 82-83 0.017789942197013582 84-85 0.014091301213154243 86-87 0.0169824924047626 88-89 0.016722024729842928 90-91 0.012606635466112114 92-93 0.009585210437043922 94-95 0.008361012364921464 96-97 0.009897771646947526 98-99 0.006876346617879334 100-101 0.010601034369230641 102-103 0.012163840418748672 104-105 0.011174063254053918 106-107 0.005756335615724746 108-109 0.0034121265414477006 110-111 0.007319141665242777 112-113 0.00932474276212425 114-115 0.014195488283122112 116-117 0.011825232441353098 118-119 0.22009518530712266 120-121 0.02724491879659767 122-123 0.02852121040370406 124-125 0.23715581801436117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1919624.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.33564026899437 #Duplication Level Percentage of deduplicated Percentage of total 1 86.99614862431856 61.1892981442804 2 7.763784761118081 10.921415441678034 3 2.3321643019138687 4.921028081628127 4 0.9699974273700124 2.729015604533887 5 0.5123342829764072 1.801767991245087 6 0.2978037369521832 1.256772990781859 7 0.2077088999494656 1.0226536927259908 8 0.14561906355323592 0.8193768056310585 9 0.10604223801753863 0.6712693835868608 >10 0.578233650330846 7.848890678293681 >50 0.059182445978597134 2.9269107001096306 >100 0.029997057482972773 3.461211665208545 >500 9.835100381587732E-4 0.43038882029689746 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2093534983934354E-5 2 0.0 0.0 0.0 0.0 5.2093534983934354E-5 3 0.0 0.0 0.0 0.0 5.2093534983934354E-5 4 0.0 0.0 0.0 0.0 5.2093534983934354E-5 5 0.0 5.2093534983934354E-5 0.0 5.2093534983934354E-5 1.5628060495180305E-4 6 5.2093534983934354E-5 5.2093534983934354E-5 0.0 5.2093534983934354E-5 1.5628060495180305E-4 7 1.0418706996786871E-4 5.2093534983934354E-5 0.0 5.2093534983934354E-5 1.5628060495180305E-4 8 1.0418706996786871E-4 5.2093534983934354E-5 0.0 5.2093534983934354E-5 1.5628060495180305E-4 9 1.0418706996786871E-4 5.2093534983934354E-5 0.0 1.0418706996786871E-4 1.5628060495180305E-4 10-11 1.0418706996786871E-4 5.2093534983934354E-5 0.0 2.0837413993573742E-4 1.5628060495180305E-4 12-13 1.5628060495180305E-4 5.2093534983934354E-5 0.0 3.646547448875405E-4 5.99075652315245E-4 14-15 2.0837413993573742E-4 5.2093534983934354E-5 0.0 3.9070151237950766E-4 6.251224198072122E-4 16-17 2.0837413993573742E-4 5.2093534983934354E-5 0.0 4.1674827987147483E-4 6.251224198072122E-4 18-19 2.0837413993573742E-4 5.2093534983934354E-5 0.0 4.1674827987147483E-4 6.251224198072122E-4 20-21 2.0837413993573742E-4 5.2093534983934354E-5 0.0 4.1674827987147483E-4 7.814030247590153E-4 22-23 2.6046767491967176E-4 5.2093534983934354E-5 0.0 4.948885823473763E-4 7.814030247590153E-4 24-25 3.125612099036061E-4 5.2093534983934354E-5 0.0 7.814030247590153E-4 7.814030247590153E-4 26-27 3.125612099036061E-4 5.2093534983934354E-5 0.0 0.0012241980721224574 8.334965597429497E-4 28-29 4.42795047363442E-4 5.2093534983934354E-5 0.0 0.003698640983859339 8.334965597429497E-4 30-31 4.688418148554092E-4 5.2093534983934354E-5 0.0 0.011408484161481623 8.334965597429497E-4 32-33 4.688418148554092E-4 5.2093534983934354E-5 0.0 0.029745408475826515 9.376836297108183E-4 34-35 4.688418148554092E-4 5.2093534983934354E-5 0.0 0.05779777706467516 0.001041870699678687 36-37 5.209353498393435E-4 5.2093534983934354E-5 0.0 0.10455172471275626 0.001041870699678687 38-39 5.730288848232779E-4 1.0418706996786871E-4 0.0 0.17461752926614793 0.001041870699678687 40-41 5.730288848232779E-4 1.0418706996786871E-4 0.0 0.24885081661825442 0.001041870699678687 42-43 5.99075652315245E-4 1.0418706996786871E-4 0.0 0.3265222772793005 0.0011200110021545885 44-45 6.251224198072122E-4 1.0418706996786871E-4 0.0 0.42195763336986825 0.0011460577696465557 46-47 6.251224198072122E-4 1.0418706996786871E-4 0.0 0.5326043016757449 0.0011721045371385229 48-49 6.251224198072122E-4 1.0418706996786871E-4 0.0 0.651481748509083 0.0012502448396144244 50-51 6.251224198072122E-4 1.0418706996786871E-4 0.0 0.7879147166320071 0.001302338374598359 52-53 6.251224198072122E-4 1.0418706996786871E-4 0.0 0.9133819956408129 0.001458618979550162 54-55 6.251224198072122E-4 1.0418706996786871E-4 0.0 1.0513517230457632 0.001458618979550162 56-57 6.772159547911466E-4 1.0418706996786871E-4 0.0 1.2145347213829374 0.0015367592820260635 58-59 6.772159547911466E-4 1.0418706996786871E-4 0.0 1.389568998928957 0.0015628060495180307 60-61 6.772159547911466E-4 1.0418706996786871E-4 0.0 1.5860397661208654 0.0015628060495180307 62-63 6.772159547911466E-4 1.0418706996786871E-4 0.0 1.7757383737648622 0.0015628060495180307 64-65 6.772159547911466E-4 1.0418706996786871E-4 0.0 1.9708286622796964 0.001614899584501965 66-67 6.772159547911466E-4 1.0418706996786871E-4 0.0 2.199024392276821 0.0016409463519939322 68-69 6.772159547911466E-4 1.5628060495180305E-4 0.0 2.4554808650027296 0.0016669931194858993 70-71 7.553562572670482E-4 1.5628060495180305E-4 0.0 2.7307951973928226 0.001771180189453768 72-73 8.334965597429497E-4 1.5628060495180305E-4 0.0 3.0136630923555865 0.0018753672594216367 74-75 8.334965597429497E-4 1.5628060495180305E-4 0.0 3.2968175017607617 0.001927460794405571 76-77 8.334965597429497E-4 1.5628060495180305E-4 0.0 3.6053414627031124 0.001927460794405571 78-79 8.595433272349168E-4 1.5628060495180305E-4 0.0 3.9529876684183987 0.001927460794405571 80-81 0.001041870699678687 1.5628060495180305E-4 0.0 4.333062099661184 0.001953507561897538 82-83 0.001041870699678687 1.5628060495180305E-4 0.0 4.7286864510966735 0.0020316478643734397 84-85 0.001041870699678687 1.5628060495180305E-4 0.0 5.123190791530008 0.0020316478643734397 86-87 0.001041870699678687 1.5628060495180305E-4 0.0 5.524337057673794 0.002083741399357374 88-89 0.001041870699678687 1.5628060495180305E-4 0.0 5.95035798677241 0.0021358349343413084 90-91 0.0010939642346626214 1.5628060495180305E-4 0.0 6.434932049192967 0.0021358349343413084 92-93 0.0010939642346626214 1.5628060495180305E-4 0.0 6.92713781448867 0.0023442090742770458 94-95 0.0010939642346626214 1.5628060495180305E-4 0.0 7.428876696686435 0.002370255841769013 96-97 0.0010939642346626214 1.5628060495180305E-4 0.0 7.936345867732431 0.002604676749196718 98-99 0.0011200110021545885 2.0837413993573742E-4 0.0 8.461162185928078 0.0028130508891324553 100-101 0.0011460577696465557 2.6046767491967176E-4 0.0 9.052423807995734 0.0028130508891324553 102-103 0.0011460577696465557 2.6046767491967176E-4 0.0 9.67535829933362 0.0028651444241163896 104-105 0.0012502448396144244 2.8651444241163893E-4 0.0 10.291676911728548 0.002917237959100324 106-107 0.0012502448396144244 3.125612099036061E-4 0.0 10.906432718073956 0.002917237959100324 108-109 0.0012762916071063918 3.125612099036061E-4 0.0 11.546532029189049 0.002917237959100324 110-111 0.0013544319095822933 3.125612099036061E-4 0.0 12.228670823036179 0.002917237959100324 112-113 0.0013544319095822933 3.125612099036061E-4 0.0 12.952849099615342 0.0029693314940842583 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1480 0.0 57.915283 1 GTATCAA 2680 0.0 47.75249 1 ATCAACG 2725 0.0 43.209938 3 CAACGCA 2760 0.0 43.099648 5 TCAACGC 2855 0.0 41.658997 4 GTACATG 7945 0.0 39.707664 1 AACGCAG 3030 0.0 39.062794 6 TACATGG 8500 0.0 36.730373 2 TATCAAC 3430 0.0 36.062363 2 ACATGGG 8370 0.0 35.87987 3 CATGGGA 4795 0.0 32.617603 4 ACGCAGA 3710 0.0 32.06335 7 CGCAGAG 3855 0.0 30.548761 8 CATGGGG 3935 0.0 29.167387 4 GTATACG 160 8.717951E-6 26.041765 1 GAGTACA 5465 0.0 25.378044 1 ATGGGAG 2480 0.0 24.942184 5 GTGTAAG 1105 0.0 24.77924 1 GCAGAGT 4795 0.0 23.568348 9 ATGGGAT 2350 0.0 23.53791 5 >>END_MODULE