FastQCFastQC Report
Thu 26 May 2016
SRR1364432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919624
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21180.11033410709597297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.10142611261372018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.067.421691
GTATCAA44400.053.5432471
ATCAACG55650.041.6278533
TCAACGC56800.040.680194
CAACGCA57350.040.254375
TATCAAC60350.038.4876062
AACGCAG60850.037.9370276
GTACATG77400.034.5636941
TACATGG82100.032.1361732
ACGCAGA72450.031.9450367
ACATGGG80550.031.8648093
CGCAGAG73500.031.406918
CATGGGA44750.029.9425724
TAAGGTG12350.028.9067385
GTGGTAT15100.028.409941
TGGTATC14300.027.9043772
CCGTTGC3000.027.7643599
GTAAGGT11250.027.5264634
GCAGAGT87750.025.8998419
TACCGTA1150.001005393925.868097
Produced by FastQC (version 0.11.5)