FastQCFastQC Report
Thu 26 May 2016
SRR1364431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863629
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.053.1008871
ATCAACG24850.044.0383453
TCAACGC25700.042.120114
GTACATG71350.041.8025781
CAACGCA25900.041.5685655
AACGCAG26400.040.5548826
TACATGG73400.039.8665472
GGTATCA14700.039.2838171
ACATGGG74450.038.2657663
TATCAAC30400.036.389742
CATGGGG43900.031.5676774
ACGCAGA33600.031.5104987
CGCAGAG34850.030.5509558
CATGGGA39500.028.9104864
ATGGGGA25800.027.8966395
GCAGAGT43400.024.1217699
GAGTACA54950.023.618271
ATGGGAT20200.023.5572625
GTATAGG7300.022.8346581
AGTACAT54750.022.1608122
Produced by FastQC (version 0.11.5)