##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364431_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1863629 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.58313162115421 31.0 30.0 34.0 25.0 34.0 2 29.654730635764952 31.0 30.0 34.0 25.0 34.0 3 29.593514589008866 31.0 30.0 34.0 24.0 34.0 4 33.15333041072016 35.0 33.0 37.0 26.0 37.0 5 33.271095266278856 35.0 35.0 37.0 27.0 37.0 6 33.382946391154036 37.0 35.0 37.0 27.0 37.0 7 33.225877575418714 37.0 35.0 37.0 27.0 37.0 8 33.09193729009368 37.0 35.0 37.0 26.0 37.0 9 34.09295090385479 38.0 34.0 39.0 23.0 39.0 10-11 34.23926596978261 38.0 34.0 39.0 25.5 39.0 12-13 34.268578402675644 38.0 34.0 39.0 25.5 39.0 14-15 35.26914128294848 39.0 34.0 41.0 25.0 41.0 16-17 35.37893325334603 39.0 35.0 41.0 26.0 41.0 18-19 35.329271008339106 39.0 34.0 41.0 25.0 41.0 20-21 35.254655298881914 39.0 34.0 41.0 25.0 41.0 22-23 35.13839879074644 39.0 34.0 41.0 24.5 41.0 24-25 35.15910114083866 39.0 34.0 41.0 24.5 41.0 26-27 35.13232542528583 39.0 34.0 41.0 25.0 41.0 28-29 35.05905198942493 39.0 34.0 41.0 24.5 41.0 30-31 34.83165104213339 39.0 34.0 41.0 24.0 41.0 32-33 34.88323051422789 39.0 34.0 41.0 24.0 41.0 34-35 34.81586490658817 39.0 34.0 41.0 24.0 41.0 36-37 34.685733050945224 39.0 34.0 41.0 24.0 41.0 38-39 34.52939614054085 39.0 33.0 41.0 23.0 41.0 40-41 34.490310303177296 39.0 33.0 41.0 23.0 41.0 42-43 34.3401613196618 38.5 33.0 40.5 22.5 41.0 44-45 34.314537120853984 38.0 33.0 40.0 22.5 41.0 46-47 34.156933059101355 38.0 33.0 40.0 22.0 41.0 48-49 33.97999575022711 38.0 33.0 40.0 21.0 41.0 50-51 33.82198119904767 38.0 33.0 40.0 20.0 41.0 52-53 33.665135335412785 38.0 33.0 40.0 20.0 41.0 54-55 33.52128159628338 38.0 32.5 40.0 19.5 41.0 56-57 33.46931282996776 38.0 32.5 40.0 19.5 41.0 58-59 33.44870545586058 37.5 32.5 40.0 19.0 41.0 60-61 33.26249323229033 37.0 32.0 40.0 18.0 41.0 62-63 33.006875563752224 37.0 32.0 40.0 18.0 41.0 64-65 32.71056846614857 36.0 32.0 40.0 15.5 41.0 66-67 32.41029625531691 36.0 31.5 39.0 13.0 41.0 68-69 32.11892844552215 35.0 31.0 39.0 12.5 41.0 70-71 31.7849947602232 35.0 31.0 38.5 9.0 40.0 72-73 31.46176787332672 35.0 31.0 37.5 8.0 40.0 74-75 31.113822547298845 35.0 31.0 37.0 7.0 39.0 76-77 30.778214709043485 35.0 30.5 36.5 4.5 39.0 78-79 30.470673615832336 35.0 30.0 36.0 2.0 39.0 80-81 30.162179006658512 35.0 30.0 36.0 2.0 37.0 82-83 29.885866768546745 34.0 30.0 35.0 2.0 37.0 84-85 29.56802695171625 34.0 30.0 35.0 2.0 36.5 86-87 29.372818838942727 34.0 29.0 35.0 2.0 36.0 88-89 29.239317213887528 34.0 29.0 35.0 2.0 36.0 90-91 29.1154720172309 34.0 29.0 35.0 2.0 36.0 92-93 28.898364964271323 34.0 29.0 35.0 2.0 35.0 94-95 28.820381631751815 34.0 29.0 35.0 2.0 35.0 96-97 28.750458648153682 34.0 29.0 35.0 2.0 35.0 98-99 28.60628054188897 34.0 29.0 35.0 2.0 35.0 100-101 28.483688813599706 34.0 29.0 35.0 2.0 35.0 102-103 27.47412897094862 32.5 26.0 34.5 2.0 35.0 104-105 28.412586142413538 34.0 29.0 35.0 2.0 35.0 106-107 28.67017040408794 34.0 29.0 35.0 2.0 35.0 108-109 28.666596463137246 34.0 29.0 35.0 2.0 35.0 110-111 28.575333931807243 34.0 29.0 35.0 2.0 35.0 112-113 28.462566852093417 34.0 29.0 35.0 2.0 35.0 114-115 28.365352492368384 34.0 29.0 35.0 2.0 35.0 116-117 28.24340627882481 34.0 29.0 35.0 2.0 35.0 118-119 27.98473113479131 34.0 29.0 35.0 2.0 35.0 120-121 27.844876313901533 34.0 29.0 35.0 2.0 35.0 122-123 27.606512615976676 34.0 29.0 35.0 2.0 35.0 124-125 26.42398621184796 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2802440940297934 1101 2 1.3394099283525094 1101 3 1.4174882592921314 1101 4 1.35975543094726 1101 5 1.3213885599499662 1101 6 1.3094404255644534 1101 7 1.3986128273630527 1101 8 1.4020385270154136 1101 9 1.951028357832861 1101 10-11 1.7709284484492116 1101 12-13 1.6621909948074105 1101 14-15 1.9167918673805104 1101 16-17 1.6176339813642784 1101 18-19 1.7156373184879712 1101 20-21 1.6867044315576365 1101 22-23 1.8812915040288658 1101 24-25 1.8688127166438022 1101 26-27 1.8574162865538 1101 28-29 1.7906614541943924 1101 30-31 1.990598081323867 1101 32-33 1.8775354270759834 1101 34-35 1.7152873353354323 1101 36-37 1.7335247911818001 1101 38-39 1.9324197659132523 1101 40-41 1.8326382036590303 1101 42-43 1.8081711252517394 1101 44-45 1.6816960933071243 1101 46-47 1.7433823176472956 1101 48-49 1.6433591593411094 1101 50-51 1.7154721474923775 1101 52-53 1.5810932584486466 1101 54-55 1.4395546435758177 1101 56-57 1.5505921692771736 1101 58-59 1.7194938555694534 1101 60-61 1.5111554638512956 1101 62-63 1.6101993457878478 1101 64-65 1.3868828390193784 1101 66-67 1.576597585075966 1101 68-69 1.4720675935819898 1101 70-71 1.4641017285427829 1101 72-73 1.479123370954916 1101 74-75 1.5585562437839826 1101 76-77 1.5294019346969243 1101 78-79 1.3776053188285822 1101 80-81 1.508170720722429 1101 82-83 1.4184320655532012 1101 84-85 1.624542045125903 1101 86-87 1.593117974398801 1101 88-89 1.4492005808631632 1101 90-91 1.4879454481574115 1101 92-93 1.5364621923815598 1101 94-95 1.5289823293407387 1101 96-97 1.480814249108814 1101 98-99 1.5681482437497003 1101 100-101 1.4881208413027345 1101 102-103 1.3575313637863324 1101 104-105 1.4136646876969223 1101 106-107 1.3066756419557244 1101 108-109 1.332342314752033 1101 110-111 1.3518948638340582 1101 112-113 1.2358857959286347 1101 114-115 1.3290470345124987 1101 116-117 1.3883858562245734 1101 118-119 1.2901338374889697 1101 120-121 1.2291549676216746 1101 122-123 1.2569507639870672 1101 124-125 0.984858168078798 1102 1 0.6026201032570135 1102 2 0.5378915685942083 1102 3 0.6024657445794865 1102 4 0.45511526056831286 1102 5 0.4660306485676813 1102 6 0.4828929848146686 1102 7 0.7315044739251064 1102 8 0.640891715349575 1102 9 1.0420487870756716 1102 10-11 0.729921176356882 1102 12-13 0.7337569170498739 1102 14-15 0.9059502524202117 1102 16-17 0.7228647479465948 1102 18-19 0.6999118184547726 1102 20-21 0.8025926129935073 1102 22-23 0.8581226227801437 1102 24-25 0.7931709059247822 1102 26-27 0.7539677605235511 1102 28-29 0.6942711860590407 1102 30-31 0.9052107569472341 1102 32-33 0.790222854988329 1102 34-35 0.6687293442724567 1102 36-37 0.67419919966337 1102 38-39 0.7476866101399722 1102 40-41 0.7055706250911626 1102 42-43 0.8051950269985682 1102 44-45 0.6864706633634086 1102 46-47 0.6827110078591119 1102 48-49 0.6538364319007002 1102 50-51 0.5260153287683949 1102 52-53 0.5506586789979053 1102 54-55 0.46309893398732527 1102 56-57 0.5414932004534734 1102 58-59 0.6372708873654886 1102 60-61 0.5809895607912097 1102 62-63 0.5640000407805132 1102 64-65 0.60420637949791 1102 66-67 0.5318362213557677 1102 68-69 0.49308915409381626 1102 70-71 0.6153623557084948 1102 72-73 0.6157213991038475 1102 74-75 0.5634493511586243 1102 76-77 0.6476542224425792 1102 78-79 0.5845862323529012 1102 80-81 0.7600385442346216 1102 82-83 0.6812108557347685 1102 84-85 0.8076790049142097 1102 86-87 0.8031266310660321 1102 88-89 0.7542374331809114 1102 90-91 0.6548586576905642 1102 92-93 0.5651268276825618 1102 94-95 0.7022966770288903 1102 96-97 0.6793524528011794 1102 98-99 0.6542235518360577 1102 100-101 0.6177792718391295 1102 102-103 0.5123157709644808 1102 104-105 0.6258125494755227 1102 106-107 0.6222787927858562 1102 108-109 0.5614836815709268 1102 110-111 0.574425807730492 1102 112-113 0.6043595418800436 1102 114-115 0.5041457131411065 1102 116-117 0.56412603816214 1102 118-119 0.6717406329727496 1102 120-121 0.5515831135947273 1102 122-123 0.5892300497136524 1102 124-125 0.0878227077137943 1103 1 0.635953436590345 1103 2 0.6741717063668844 1103 3 0.7873529966309185 1103 4 0.6600143415867947 1103 5 0.6986552379086888 1103 6 0.6351621087255808 1103 7 0.7323389628192984 1103 8 0.7972353716932332 1103 9 1.332515037243411 1103 10-11 1.0047900885392238 1103 12-13 0.8841112890460394 1103 14-15 1.1932721345182173 1103 16-17 0.972338532868136 1103 18-19 1.019968627317553 1103 20-21 0.9635137813773653 1103 22-23 0.9833621842137319 1103 24-25 0.7845300442988474 1103 26-27 1.0054466299128677 1103 28-29 0.8223265883681705 1103 30-31 1.0453395336431726 1103 32-33 1.003800943566418 1103 34-35 1.018289609877499 1103 36-37 0.9872178973835588 1103 38-39 0.9426600446656437 1103 40-41 0.9547901866241517 1103 42-43 0.9898501728477456 1103 44-45 0.7747678139739946 1103 46-47 0.7836157001817199 1103 48-49 0.7886963481039047 1103 50-51 0.7595099436062966 1103 52-53 0.7506585547440494 1103 54-55 0.8068375336960685 1103 56-57 0.7427474188718719 1103 58-59 0.8436185198325319 1103 60-61 0.9088845513727719 1103 62-63 0.95032565546434 1103 64-65 0.9338714905063057 1103 66-67 0.7090563892475714 1103 68-69 0.7655421985617892 1103 70-71 0.7875210427925516 1103 72-73 0.7289467309858964 1103 74-75 0.8680155560979657 1103 76-77 0.8021975646724862 1103 78-79 0.7394075304577861 1103 80-81 0.758886959500952 1103 82-83 0.6212558853320402 1103 84-85 0.7157935272078362 1103 86-87 0.7834378621235274 1103 88-89 0.7444766715544766 1103 90-91 0.8122636904687326 1103 92-93 0.881042007030576 1103 94-95 0.7918328870874909 1103 96-97 0.8172450328579366 1103 98-99 0.8815843750924053 1103 100-101 0.8984252427836061 1103 102-103 0.9482329632249531 1103 104-105 0.8138649199905785 1103 106-107 0.8162414221962422 1103 108-109 0.8228424720341962 1103 110-111 0.871061510336343 1103 112-113 0.8970679528720353 1103 114-115 0.8455580321653642 1103 116-117 0.6608554281751111 1103 118-119 0.5939213129892487 1103 120-121 0.5937362341060535 1103 122-123 0.7222204424055398 1103 124-125 0.4759203066322861 1104 1 0.6038391906829972 1104 2 0.6444018593556571 1104 3 0.7873529966309185 1104 4 0.6047347552727231 1104 5 0.7013104371156089 1104 6 0.5449067701943804 1104 7 0.6078393245265516 1104 8 0.8531124448116856 1104 9 1.2028684386312989 1104 10-11 0.9575886309929444 1104 12-13 0.9073047622395194 1104 14-15 1.0023014194095694 1104 16-17 0.9738355990203758 1104 18-19 0.9291734072119837 1104 20-21 1.0109912081651373 1104 22-23 1.0038796935243468 1104 24-25 1.0566517922826648 1104 26-27 0.8980289518055358 1104 28-29 0.9560008642838298 1104 30-31 1.0728185680187607 1104 32-33 1.0720062057371962 1104 34-35 1.0424804573442046 1104 36-37 0.7694493632357151 1104 38-39 1.0607427282655664 1104 40-41 0.9103490913209953 1104 42-43 0.9171162018379135 1104 44-45 0.8309416210098703 1104 46-47 0.810150946544546 1104 48-49 0.7577342067969326 1104 50-51 0.818908973694974 1104 52-53 0.6572302397640044 1104 54-55 0.6944095402335932 1104 56-57 0.7121121537538428 1104 58-59 0.7513751310374133 1104 60-61 0.936182732119228 1104 62-63 0.9707875690296959 1104 64-65 0.8555390949325243 1104 66-67 0.8093598214475577 1104 68-69 0.7680145346548102 1104 70-71 0.8459501615664955 1104 72-73 0.7492719995870303 1104 74-75 0.7663470139121813 1104 76-77 0.7280053775492696 1104 78-79 0.5174036722470277 1104 80-81 0.6997638986537531 1104 82-83 0.728468595993025 1104 84-85 0.6770801333220291 1104 86-87 0.7192468806911698 1104 88-89 0.8173104469744601 1104 90-91 0.7301253307441655 1104 92-93 0.7572617642848165 1104 94-95 0.683345493255267 1104 96-97 0.6195887619258045 1104 98-99 0.7821168885559509 1104 100-101 0.6369242916274835 1104 102-103 0.6788970309579874 1104 104-105 0.6843877879272426 1104 106-107 0.554595118308562 1104 108-109 0.4837097656601088 1104 110-111 0.6370918401597798 1104 112-113 0.6164052247955176 1104 114-115 0.5835948995125761 1104 116-117 0.5656353226101771 1104 118-119 0.4954491912412635 1104 120-121 0.37373114341130886 1104 122-123 0.40106726607994503 1104 124-125 0.24924707564871085 1105 1 0.31296611216417247 1105 2 0.4286740385670562 1105 3 0.4257721314529306 1105 4 0.2860147201051575 1105 5 0.2859597798894882 1105 6 0.28892704882145637 1105 7 0.4062039467562215 1105 8 0.38532474035813635 1105 9 0.8078114434750745 1105 10-11 0.44878063037150184 1105 12-13 0.5963171886620131 1105 14-15 0.20710506417848507 1105 16-17 0.41346365474866076 1105 18-19 0.4393927283963279 1105 20-21 0.8154292519978696 1105 22-23 0.8888541143459534 1105 24-25 0.9166248080482902 1105 26-27 0.7909935769706067 1105 28-29 0.6943235961565506 1105 30-31 0.892536461415645 1105 32-33 0.7427750989074724 1105 34-35 0.6231095501290937 1105 36-37 0.8059262274228232 1105 38-39 0.8415173025157046 1105 40-41 0.8017853377560016 1105 42-43 0.7036894510755118 1105 44-45 0.7388347422495656 1105 46-47 0.757868365981146 1105 48-49 0.6724471296964083 1105 50-51 0.8023341457830142 1105 52-53 0.5674784854129058 1105 54-55 0.5666536168934471 1105 56-57 0.5921891042847776 1105 58-59 0.6760326285056237 1105 60-61 0.620865975224703 1105 62-63 0.6976713553280263 1105 64-65 0.6414878549571981 1105 66-67 0.4594783294177134 1105 68-69 0.42932140496689186 1105 70-71 0.5957290218638427 1105 72-73 0.5006735653026553 1105 74-75 0.572801357826215 1105 76-77 0.5628303970679092 1105 78-79 0.48331074954085906 1105 80-81 0.45372964678967875 1105 82-83 0.4853019112485697 1105 84-85 0.5790367587963914 1105 86-87 0.5948693562888394 1105 88-89 0.48591179518384564 1105 90-91 0.521524813039207 1105 92-93 0.4706582660712968 1105 94-95 0.4974895206218193 1105 96-97 0.5010315700617447 1105 98-99 0.538247675820454 1105 100-101 0.3882447757686087 1105 102-103 0.35578087095271727 1105 104-105 0.3066861874521365 1105 106-107 0.3425111356961743 1105 108-109 0.32845304850808077 1105 110-111 0.38104660917036526 1105 112-113 0.4219775157312391 1105 114-115 0.42615309657337974 1105 116-117 0.4559988938221764 1105 118-119 0.28805397888450557 1105 120-121 0.29145518182500396 1105 122-123 0.37503381608221886 1105 124-125 0.20255608092993427 1106 1 0.4979689404663148 1106 2 0.5785376808036382 1106 3 0.6212225312082396 1106 4 0.4605866038375197 1106 5 0.4135392416703425 1106 6 0.3610814876277715 1106 7 0.3661314258331956 1106 8 0.6731513330490273 1106 9 1.0076063093013161 1106 10-11 0.5732202091432583 1106 12-13 0.6636089568652466 1106 14-15 1.0825655509097132 1106 16-17 0.8036463807331131 1106 18-19 0.7618867193367507 1106 20-21 0.8580789398454556 1106 22-23 0.7537889179114501 1106 24-25 0.693497921719711 1106 26-27 0.8148228452041479 1106 28-29 0.8150598830478799 1106 30-31 0.9449159935836349 1106 32-33 0.8783590194642272 1106 34-35 0.8671223999388005 1106 36-37 0.8879029975883768 1106 38-39 1.009567118043897 1106 40-41 0.9995654063488217 1106 42-43 0.9312768452653231 1106 44-45 0.8497417699135781 1106 46-47 1.0284773013375528 1106 48-49 0.9261177739985342 1106 50-51 0.8410647386595684 1106 52-53 0.7428484595477016 1106 54-55 0.7834425431120025 1106 56-57 0.7870663961534348 1106 58-59 0.8452917034066232 1106 60-61 0.8988321332819282 1106 62-63 0.938953239541874 1106 64-65 0.9919964124330463 1106 66-67 0.9234116750133765 1106 68-69 1.0006432872270778 1106 70-71 0.970051606263457 1106 72-73 0.8191008976820271 1106 74-75 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2216 108-109 -1.952664186911953 2216 110-111 -1.902010680211962 2216 112-113 -2.129735140531377 2216 114-115 -2.039667013489616 2216 116-117 -1.9882409372429102 2216 118-119 -1.8047365975715266 2216 120-121 -1.7901720550570097 2216 122-123 -1.7380897887645652 2216 124-125 -1.6618714398378032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63717.0 3 11661.0 4 7198.0 5 6103.0 6 6229.0 7 7142.0 8 8073.0 9 8586.0 10 9024.0 11 9444.0 12 10007.0 13 10221.0 14 10336.0 15 9898.0 16 9195.0 17 8621.0 18 7947.0 19 7817.0 20 7748.0 21 8015.0 22 9451.0 23 12615.0 24 17217.0 25 22654.0 26 27082.0 27 30896.0 28 36210.0 29 42362.0 30 50228.0 31 58647.0 32 71804.0 33 91292.0 34 120955.0 35 173397.0 36 297166.0 37 434250.0 38 140198.0 39 223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.817184020055755 18.08847325657395 12.087857792309274 25.006484931061017 2 15.781340130122745 19.705251861291835 39.168233952646055 25.345174055939367 3 18.75303507296785 24.293247207464578 29.063939228247683 27.889778491319895 4 12.473720190727828 15.545627329499172 36.807401596267006 35.17325088350599 5 13.465485517616624 38.44320348313407 34.44563747009163 13.645673529157673 6 33.595886294660296 35.916579739260705 17.06406442501277 13.423469541066224 7 29.4413212071716 32.05439494663369 20.796467537261975 17.707816308932735 8 26.810101464307735 35.34809571310751 19.678194644697847 18.163608177886907 9 26.92210487003527 15.551498936747333 19.27570037040793 38.25069582280947 10-11 25.228427172295255 26.216514050957645 27.787605727979216 20.76745304876789 12-13 25.53368110305408 23.52825018599858 27.79206472240802 23.14600398853932 14-15 22.59140250587188 25.254877402882574 24.70699629683887 27.446723794406676 16-17 23.208477008256143 28.461291212299756 25.543737812449358 22.786493966994744 18-19 23.19149861057892 27.38096004756983 26.616456455682886 22.811084886168366 20-21 23.574209174575838 26.783585275618588 26.7025995141391 22.939606035666475 22-23 23.547540761912224 26.61750319167262 26.541997796551865 23.29295824986329 24-25 23.3611424603296 26.81447577219699 26.5382896045276 23.286092162945813 26-27 23.170146307798852 27.097759540246784 26.348846712884193 23.383247439070168 28-29 23.221951879176338 27.079849700237617 26.482712951494946 23.215485469091096 30-31 23.30203668387003 26.640068673913493 26.54274127307031 23.515153369146166 32-33 23.035243658253037 26.92846872705194 26.51391794357484 23.522369671120185 34-35 23.634322619833988 26.876758358423675 26.161261086746666 23.32765793499567 36-37 23.758703509735913 26.572856210721525 26.192627665785228 23.47581261375734 38-39 23.185342962245326 26.738984564210526 26.502295125598064 23.573377347946085 40-41 23.411292722117988 26.52314862884897 26.55805421607712 23.50750443295592 42-43 23.382973385938104 26.466952764366482 26.459815972729945 23.690257876965468 44-45 23.24750987665086 26.710407877174035 26.485226944979175 23.55685530119593 46-47 23.497100769586574 27.027449052853676 26.069490308532206 23.405959869027548 48-49 23.476068595997287 26.69287774391905 26.253695072315686 23.57735858776798 50-51 23.395695776026454 26.743327154238315 26.362171167103064 23.498805902632167 52-53 23.565558595271852 26.675952171155227 26.205729286849643 23.552759946723278 54-55 23.250799521427496 26.556588193404895 26.342389701729342 23.850222583438267 56-57 23.179440367750363 27.006372092873494 26.43849701465785 23.37569052471829 58-59 23.384976416227097 26.475699685022004 26.34199703938392 23.79732685936698 60-61 23.21038304517628 26.6008648065589 26.715841415459995 23.472910732804824 62-63 23.09006926175534 26.702322571089095 26.62936320615488 23.57824496100068 64-65 23.033436984577886 26.628334756760342 26.468444058782037 23.86978419987974 66-67 23.008661487680744 26.832070071137487 26.3376147488131 23.821653692368667 68-69 22.946770070769666 26.39724069682387 26.872401547948904 23.78358768445756 70-71 22.983938693255386 26.43770186638706 26.69033837085944 23.888021069498112 72-73 23.075430744754872 26.521093619817627 26.426936335561642 23.976539299865863 74-75 22.97322534959565 26.770679098642404 26.391338904252354 23.864756647509587 76-77 23.1266657025391 26.59028057453775 26.44550045330989 23.837553269613267 78-79 23.097513150113606 26.520647905372847 26.664706179077662 23.71713276543589 80-81 23.12327864347983 26.755542150828937 26.548496368917156 23.572682836774078 82-83 23.08910164176549 26.72139576095946 26.48364540527312 23.70585719200193 84-85 23.177371929415944 26.452974642139736 26.54018236879799 23.82947105964633 86-87 23.092137887508077 26.318482747961163 26.787834162321367 23.801545202209397 88-89 23.094230869857473 26.445556257706325 26.667391178929034 23.792821693507165 90-91 23.0537354771247 26.66284389822563 26.671510867386516 23.611909757263145 92-93 23.109028590563614 26.08314054545152 26.818068879876915 23.989761984107947 94-95 22.90944450049263 26.22526396905568 26.88255711369505 23.982734416756642 96-97 23.018763836890844 26.14755468548551 26.834103275561503 23.999578202062143 98-99 23.050143573226638 26.200302762154625 26.619807041447075 24.12974662317166 100-101 22.757578489582663 26.04964924934207 27.017352927286854 24.17541933378841 102-103 22.778370931911482 25.76698228310169 27.079133439823806 24.375513345163025 104-105 22.594091626002253 25.996378707357376 27.026123429838528 24.383406236801843 106-107 22.78281853566736 26.014585747544377 26.85328903750755 24.349306679280712 108-109 22.799487648948308 25.981827313870287 26.65140381397815 24.567281223203256 110-111 22.645872803224687 26.216602803812293 26.957440534564427 24.1800838583986 112-113 22.647467142916174 26.11426446336873 27.177159128046966 24.061109265668122 114-115 22.376033213084735 26.285982004570236 26.891178418364227 24.446806363980805 116-117 22.39045101122866 26.127621985921984 27.1837819171016 24.29814508574776 118-119 22.311078225158777 26.255367008034682 26.84467921094866 24.588875555857886 120-121 22.340516032595204 26.39779514077581 26.96087153125809 24.300817295370898 122-123 22.4201233691561 26.412539265716017 26.938085966590762 24.229251398537123 124-125 22.365636276309484 26.460807319889657 26.81492612933189 24.358630274468972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 308.0 1 259.0 2 183.5 3 144.5 4 120.0 5 102.0 6 93.0 7 86.0 8 86.0 9 83.5 10 87.5 11 94.5 12 107.5 13 121.0 14 124.5 15 154.0 16 188.5 17 213.0 18 272.5 19 339.0 20 468.5 21 676.5 22 950.5 23 1494.0 24 2285.5 25 3342.0 26 4911.5 27 7136.0 28 9602.0 29 12336.5 30 16152.0 31 20231.5 32 24742.5 33 28481.0 34 31246.0 35 34730.0 36 39923.0 37 45015.0 38 48514.0 39 51456.5 40 53179.0 41 57001.0 42 61440.0 43 64953.5 44 68886.5 45 72790.0 46 73729.0 47 72197.0 48 72226.5 49 71328.5 50 70350.5 51 69471.0 52 69287.0 53 69978.5 54 70638.5 55 67443.0 56 60071.5 57 54903.5 58 51176.5 59 44928.0 60 37059.0 61 30876.5 62 25737.0 63 20642.5 64 16247.5 65 12706.0 66 9534.5 67 7842.0 68 6082.5 69 3868.0 70 2775.0 71 2082.0 72 1562.5 73 1191.5 74 912.0 75 698.5 76 473.0 77 270.0 78 140.5 79 81.5 80 42.0 81 27.5 82 17.5 83 12.5 84 11.0 85 6.5 86 5.0 87 2.5 88 5.0 89 4.5 90 1.5 91 4.5 92 4.0 93 2.0 94 1.5 95 1.5 96 2.0 97 2.5 98 2.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08692717273663374 2 2.1463499441144135E-4 3 0.0 4 0.0016634212066886703 5 0.0010731749720572067 6 2.682937430143017E-4 7 0.0 8 9.65857474851486E-4 9 4.292699888228827E-4 10-11 0.00963174537421343 12-13 0.002119520569812983 14-15 0.004614652379845989 16-17 0.002521961184334436 18-19 0.014112250882552269 20-21 0.018163486402068222 22-23 0.009980527240132021 24-25 0.01137565470380639 26-27 0.010436626603256335 28-29 0.00820978853623763 30-31 0.01773421641324534 32-33 0.002441473061430145 34-35 0.00160976245808581 36-37 6.975637318371843E-4 38-39 2.9512311731573184E-4 40-41 0.0016365918323872403 42-43 0.0024951318100330053 44-45 8.853693519471955E-4 46-47 0.0016634212066886703 48-49 0.008987840390979105 50-51 0.004695140502750279 52-53 0.008102471039031911 54-55 0.009148816636787687 56-57 0.010248820983146324 58-59 0.008880522893773385 60-61 0.01108053158649066 62-63 0.014112250882552269 64-65 0.009041499139581967 66-67 0.014246397754059418 68-69 0.013951274636743689 70-71 0.004400017385434548 72-73 0.013817127765236534 74-75 0.014058592133949407 76-77 0.0060902679664246485 78-79 0.00788783604462047 80-81 0.014648838368580872 82-83 0.01770738703894391 84-85 0.014058592133949407 86-87 0.016795188312695285 88-89 0.016446406446776692 90-91 0.012475659050165027 92-93 0.00976589224572058 94-95 0.00820978853623763 96-97 0.009524427877007708 98-99 0.00643904983234324 100-101 0.010570773474763486 102-103 0.012797611541782189 104-105 0.01105370221218923 106-107 0.005231727988778882 108-109 0.003380501161980201 110-111 0.0070024666926732735 112-113 0.00965857474851486 114-115 0.014326885876963708 116-117 0.012207365307150726 118-119 0.21482280003155135 120-121 0.02530009996624865 122-123 0.027983037396391665 124-125 0.2342740964000882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1863629.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.59564241119983 #Duplication Level Percentage of deduplicated Percentage of total 1 88.21509231101034 68.45106758236142 2 7.549636828460576 11.716378393513033 3 2.0690129191572093 4.816391598572265 4 0.8080300622611231 2.5079844707485455 5 0.3889812554942264 1.5091625202994776 6 0.23353960883810276 1.0872993585751745 7 0.15857478052827073 0.8613298375714331 8 0.09468836612125207 0.5877923678436356 9 0.07193277412255915 0.5023502836613738 >10 0.3730968392312812 5.277328950240042 >50 0.027836017131453325 1.490693575523564 >100 0.00957823764354911 1.192221061090095 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 2.9512311731573184E-4 0.0 28-29 0.0 0.0 0.0 0.002307326189922994 0.0 30-31 0.0 0.0 0.0 0.008987840390979105 0.0 32-33 0.0 0.0 0.0 0.025004976848932915 0.0 34-35 0.0 0.0 0.0 0.04574408318393844 0.0 36-37 0.0 0.0 0.0 0.08140032163053912 0.0 38-39 0.0 0.0 0.0 0.14286641815511564 0.0 40-41 0.0 0.0 0.0 0.209000825808141 0.0 42-43 0.0 0.0 0.0 0.2743840109807263 0.0 44-45 0.0 0.0 0.0 0.3518404145889552 0.0 46-47 0.0 0.0 0.0 0.44649444712440084 0.0 48-49 0.0 0.0 0.0 0.5524168168664472 0.0 50-51 0.0 0.0 0.0 0.6692050832005727 0.0 52-53 0.0 0.0 0.0 0.7881933582274154 0.0 54-55 0.0 0.0 0.0 0.9092743244497699 0.0 56-57 0.0 0.0 0.0 1.0547431919121242 0.0 58-59 0.0 0.0 0.0 1.2148608977430593 0.0 60-61 0.0 0.0 0.0 1.3916128156408814 0.0 62-63 0.0 0.0 0.0 1.5704574247342147 0.0 64-65 0.0 0.0 0.0 1.7482020294811895 0.0 66-67 0.0 0.0 0.0 1.951863809803346 0.0 68-69 0.0 0.0 0.0 2.176962260192345 0.0 70-71 0.0 0.0 0.0 2.437341337787725 0.0 72-73 0.0 0.0 0.0 2.6976667566345016 0.0 74-75 0.0 0.0 0.0 2.952840935615404 0.0 76-77 0.0 0.0 0.0 3.229881054651972 0.0 78-79 0.0 0.0 0.0 3.5477554813753165 0.0 80-81 0.0 0.0 0.0 3.8979324747575834 0.0 82-83 0.0 0.0 0.0 4.2570704791565275 0.0 84-85 0.0 0.0 0.0 4.617040194158816 0.0 86-87 0.0 0.0 0.0 4.985702626434768 0.0 88-89 0.0 0.0 0.0 5.386238355380819 0.0 90-91 0.0 0.0 0.0 5.833242560616947 0.0 92-93 0.0 0.0 0.0 6.288724848132327 0.0 94-95 0.0 0.0 0.0 6.754429127256552 0.0 96-97 0.0 0.0 0.0 7.221528533844451 0.0 98-99 0.0 0.0 0.0 7.703115802555122 0.0 100-101 0.0 0.0 0.0 8.249871621443969 0.0 102-103 0.0 5.365874860286033E-5 0.0 8.839956879829622 0.0 104-105 0.0 5.365874860286033E-5 0.0 9.433798250617478 0.0 106-107 0.0 5.365874860286033E-5 0.0 10.002151715818975 0.0 108-109 0.0 5.365874860286033E-5 0.0 10.590654041120846 0.0 110-111 0.0 5.365874860286033E-5 0.0 11.23214438066804 0.0 112-113 0.0 5.365874860286033E-5 0.0 11.914093416661792 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2175 0.0 53.100887 1 ATCAACG 2485 0.0 44.038345 3 TCAACGC 2570 0.0 42.12011 4 GTACATG 7135 0.0 41.802578 1 CAACGCA 2590 0.0 41.568565 5 AACGCAG 2640 0.0 40.554882 6 TACATGG 7340 0.0 39.866547 2 GGTATCA 1470 0.0 39.283817 1 ACATGGG 7445 0.0 38.265766 3 TATCAAC 3040 0.0 36.38974 2 CATGGGG 4390 0.0 31.567677 4 ACGCAGA 3360 0.0 31.510498 7 CGCAGAG 3485 0.0 30.550955 8 CATGGGA 3950 0.0 28.910486 4 ATGGGGA 2580 0.0 27.896639 5 GCAGAGT 4340 0.0 24.121769 9 GAGTACA 5495 0.0 23.61827 1 ATGGGAT 2020 0.0 23.557262 5 GTATAGG 730 0.0 22.834658 1 AGTACAT 5475 0.0 22.160812 2 >>END_MODULE