FastQCFastQC Report
Thu 26 May 2016
SRR1364431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863629
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.058.1374551
GTATCAA35400.056.0683821
ATCAACG44300.043.9660723
TCAACGC44800.043.475384
CAACGCA45700.042.5745935
TATCAAC47400.041.3453062
AACGCAG48150.040.5296826
GTACATG69800.035.865011
TACATGG72550.033.7452772
ACATGGG72150.033.104093
ACGCAGA59850.032.9048167
CGCAGAG61850.031.7428978
CATGGGG42750.027.4475614
GCAGAGT71650.026.8199929
CATGGGA37900.026.0881044
AGTACAT55100.023.4593942
GAGTACA58800.023.1118431
CAGAGTA69000.022.5473210-11
GAGTACT39000.021.96697212-13
AGAGTAC69900.021.87400410-11
Produced by FastQC (version 0.11.5)