FastQCFastQC Report
Thu 26 May 2016
SRR1364430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162203
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17650.048.221041
GTATCAA25050.038.2528151
GTACATG68750.035.926971
ATCAACG24950.034.802953
TACATGG72350.033.1283872
TCAACGC26550.032.9311334
CAACGCA26800.032.6262055
ACATGGG73750.030.9672743
AACGCAG28550.030.626356
TATCAAC30800.029.7374532
TAAGGTA9400.025.9423854
ACGCAGA33800.025.5173347
CATGGGG47600.025.3654734
CGCAGAG35150.024.7065168
TAAGGTG11000.023.7927135
GAGTACA54900.023.308341
CATGGGA35350.022.3777334
ATGGGGA27800.020.5404745
GTATATA12100.019.6752321
GTAAGGT12550.019.4309484
Produced by FastQC (version 0.11.5)