FastQCFastQC Report
Thu 26 May 2016
SRR1364430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162203
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27480.1270925995385262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25810.11936899541809905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28200.067.797081
GTATCAA49700.054.047451
ATCAACG62450.042.516393
TCAACGC63950.041.519144
CAACGCA63950.041.400185
TATCAAC66400.040.1683582
AACGCAG66350.039.9914056
CGCAGAG79000.033.587728
ACGCAGA78900.033.5548867
GTACATG68900.032.8487971
TACATGG72150.030.778442
ACATGGG73800.029.7662433
GTAAGGT11900.028.5155624
GCAGAGT95750.027.587799
TAAGGTG12850.026.8539165
GAGTACT58950.024.72606512-13
TGGTATC14200.024.3172112
CATGGGG47600.024.1381744
GTGGTAT15300.024.1354451
CAGAGTA91700.024.07006810-11
Produced by FastQC (version 0.11.5)