FastQCFastQC Report
Thu 26 May 2016
SRR1364429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003319
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21250.044.255081
GGTATCA13800.039.6801871
ATCAACG23600.039.062443
TCAACGC25150.036.8933374
CAACGCA25200.036.8219765
GTACATG70450.035.653031
AACGCAG26450.035.0818066
TACATGG71050.034.3209652
ACATGGG70850.033.7462773
TATCAAC28450.032.8213962
CATGGGG41700.028.9548514
CGCAGAG33150.027.4530728
ACGCAGA33750.026.7887737
GTACCGT1608.766539E-626.0232226
CATGGGA36500.024.7692074
ATGGGGA23850.023.4435025
GTATTAC6150.022.2596191
GAGTACA54400.022.2106931
AGTACCG2704.561116E-822.0302415
GGACCGA4850.020.8492376
Produced by FastQC (version 0.11.5)