##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364429_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2003319 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.794428146490898 31.0 31.0 34.0 27.0 34.0 2 30.871712393283346 31.0 31.0 34.0 27.0 34.0 3 30.839842780905087 31.0 31.0 34.0 27.0 34.0 4 34.422738465516474 37.0 35.0 37.0 30.0 37.0 5 34.611050960930335 37.0 35.0 37.0 32.0 37.0 6 34.716712116243094 37.0 35.0 37.0 32.0 37.0 7 34.577515113668866 37.0 35.0 37.0 31.0 37.0 8 34.45507130916244 37.0 35.0 37.0 30.0 37.0 9 35.57086415094151 39.0 35.0 39.0 29.0 39.0 10-11 35.809943648515286 39.0 36.0 39.0 30.5 39.0 12-13 35.87202936726503 39.0 36.0 39.0 30.0 39.0 14-15 37.05116109815761 40.0 37.0 41.0 30.5 41.0 16-17 37.19437543396734 40.0 37.0 41.0 31.0 41.0 18-19 37.1744514977395 40.0 37.0 41.0 31.0 41.0 20-21 37.12051725162094 40.0 37.0 41.0 30.5 41.0 22-23 37.034884858577186 40.0 37.0 41.0 30.0 41.0 24-25 37.091087340558346 40.0 37.0 41.0 30.0 41.0 26-27 37.098029819514515 40.0 37.0 41.0 30.0 41.0 28-29 37.05403158458538 40.0 37.0 41.0 30.0 41.0 30-31 36.828679805862166 40.0 36.0 41.0 30.0 41.0 32-33 36.90659675268891 40.0 36.0 41.0 30.0 41.0 34-35 36.863351767741435 40.0 36.0 41.0 30.0 41.0 36-37 36.744866394218796 40.0 36.0 41.0 30.0 41.0 38-39 36.610354616513895 40.0 36.0 41.0 30.0 41.0 40-41 36.5805950025932 40.0 36.0 41.0 30.0 41.0 42-43 36.45068808312605 40.0 36.0 41.0 29.5 41.0 44-45 36.435697210479205 40.0 35.5 41.0 30.0 41.0 46-47 36.29831270007423 40.0 35.0 41.0 29.0 41.0 48-49 36.13583682878264 39.0 35.0 41.0 29.0 41.0 50-51 36.01335334013205 39.0 35.0 41.0 28.5 41.0 52-53 35.88871892095068 39.0 35.0 41.0 28.0 41.0 54-55 35.773017677164745 39.0 35.0 41.0 28.0 41.0 56-57 35.73627240594234 39.0 35.0 40.5 28.5 41.0 58-59 35.72018160862049 39.0 35.0 41.0 28.0 41.0 60-61 35.5369756389272 38.5 35.0 41.0 28.0 41.0 62-63 35.289725201028894 38.0 34.0 40.0 28.0 41.0 64-65 34.99598116924963 37.5 34.0 40.0 28.0 41.0 66-67 34.69352659261955 37.0 34.0 40.0 28.0 41.0 68-69 34.39236462091159 36.5 34.0 39.5 28.0 41.0 70-71 34.0406330694213 36.0 34.0 39.0 27.0 41.0 72-73 33.71263837661401 35.5 33.0 39.0 26.0 40.5 74-75 33.357230176522066 35.0 33.0 37.5 26.0 40.0 76-77 33.002805843702376 35.0 33.0 37.0 26.0 39.0 78-79 32.67892008212372 35.0 33.0 37.0 26.0 39.0 80-81 32.35620063504614 35.0 33.0 36.0 26.0 38.5 82-83 32.067377437143065 35.0 32.0 36.0 25.0 37.0 84-85 31.72507698474382 35.0 32.0 35.5 24.5 37.0 86-87 31.53740243066631 35.0 32.0 35.0 24.5 36.5 88-89 31.39287377596878 35.0 32.0 35.0 24.0 36.0 90-91 31.27120493540969 35.0 32.0 35.0 24.0 36.0 92-93 31.050531642738875 35.0 32.0 35.0 23.0 36.0 94-95 30.97417610475416 35.0 32.0 35.0 23.0 35.0 96-97 30.903848812895 35.0 32.0 35.0 23.0 35.0 98-99 30.757284037140366 35.0 32.0 35.0 21.5 35.0 100-101 30.633402618354843 35.0 31.5 35.0 20.0 35.0 102-103 29.610326662902914 33.5 29.5 34.5 19.0 35.0 104-105 30.563329904024272 34.5 31.5 35.0 20.0 35.0 106-107 30.807779739522264 35.0 32.0 35.0 22.5 35.0 108-109 30.806112755881614 35.0 32.0 35.0 20.0 35.0 110-111 30.703404699900517 35.0 32.0 35.0 19.5 35.0 112-113 30.594300757892277 35.0 32.0 35.0 18.5 35.0 114-115 30.500404828187623 35.0 32.0 35.0 18.0 35.0 116-117 30.390027748950615 35.0 32.0 35.0 16.0 35.0 118-119 30.13853834561545 35.0 32.0 35.0 2.0 35.0 120-121 30.02117261404699 35.0 32.0 35.0 2.0 35.0 122-123 29.8020060709253 35.0 32.0 35.0 2.0 35.0 124-125 28.652304001509496 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5739899009818004 1101 2 0.55335754788819 1101 3 0.7720289619143905 1101 4 0.7081294087965588 1101 5 0.5800905024688916 1101 6 0.5304096688814894 1101 7 0.5810746300955856 1101 8 0.6368480020180982 1101 9 1.1435406317142736 1101 10-11 0.87172774670411 1101 12-13 0.7462245715354783 1101 14-15 0.9167401593270768 1101 16-17 0.7634319402117811 1101 18-19 0.8636351078676086 1101 20-21 0.964725789131208 1101 22-23 0.8663864305041358 1101 24-25 0.8468615559006523 1101 26-27 0.835024183056106 1101 28-29 0.8368084489645895 1101 30-31 1.0127764463276776 1101 32-33 0.843063683673293 1101 34-35 0.8166522535471614 1101 36-37 0.7411878126063698 1101 38-39 0.8560450943618108 1101 40-41 0.8977906583809911 1101 42-43 0.8694698828215977 1101 44-45 0.7073320072679365 1101 46-47 0.6948910982816301 1101 48-49 0.7227290805080031 1101 50-51 0.7607817634633065 1101 52-53 0.561559463382082 1101 54-55 0.5085986123284414 1101 56-57 0.609950255971043 1101 58-59 0.6258982823292385 1101 60-61 0.6802203435941223 1101 62-63 0.6426747988796748 1101 64-65 0.6505718137190115 1101 66-67 0.591781065202305 1101 68-69 0.555280697194398 1101 70-71 0.5482313315632297 1101 72-73 0.5825552458784955 1101 74-75 0.6522163850144693 1101 76-77 0.687675220340239 1101 78-79 0.5442100018237781 1101 80-81 0.629994527036402 1101 82-83 0.6933181769098695 1101 84-85 0.7540898213151479 1101 86-87 0.7566804507271314 1101 88-89 0.5788839616364996 1101 90-91 0.6114393703464387 1101 92-93 0.6819498263248853 1101 94-95 0.7045804344853543 1101 96-97 0.6150529578601898 1101 98-99 0.7216704296424226 1101 100-101 0.6647807342199563 1101 102-103 0.6781756776009793 1101 104-105 0.6277198875367418 1101 106-107 0.540016324524764 1101 108-109 0.5794033940175431 1101 110-111 0.6520835695139198 1101 112-113 0.6363390957490722 1101 114-115 0.618267365391997 1101 116-117 0.5541799373396046 1101 118-119 0.607169402477048 1101 120-121 0.5507760378781441 1101 122-123 0.47125223430023766 1101 124-125 0.06452247838005931 1102 1 0.4561064972469637 1102 2 0.37522106077444306 1102 3 0.5138613533286396 1102 4 0.49440040480002523 1102 5 0.44056590978262733 1102 6 0.2433732424074222 1102 7 0.3444767745938577 1102 8 0.38303313275542905 1102 9 0.703532861043449 1102 10-11 0.5635104239264734 1102 12-13 0.5606078440351965 1102 14-15 0.6662800282988286 1102 16-17 0.4702859692593506 1102 18-19 0.44830956072058825 1102 20-21 0.5266716763000332 1102 22-23 0.5186539213015209 1102 24-25 0.49421144683974205 1102 26-27 0.4724315588578989 1102 28-29 0.46539963668769957 1102 30-31 0.5839989758426469 1102 32-33 0.472390563858859 1102 34-35 0.3340538369021502 1102 36-37 0.32051249476524646 1102 38-39 0.40655413148390096 1102 40-41 0.35514942546903683 1102 42-43 0.4243977616870538 1102 44-45 0.394975444671374 1102 46-47 0.3829634741286796 1102 48-49 0.24155113861090172 1102 50-51 0.19141721297916092 1102 52-53 0.08366708652652477 1102 54-55 0.12470258416266944 1102 56-57 0.28460060810784427 1102 58-59 0.32273819875250354 1102 60-61 0.2279168762716708 1102 62-63 0.2929687526254341 1102 64-65 0.24078868784060603 1102 66-67 0.10989021920769204 1102 68-69 0.1525086299761611 1102 70-71 0.20538232048129856 1102 72-73 0.19215532138717606 1102 74-75 0.12957105342921693 1102 76-77 0.18906046201327342 1102 78-79 0.14168036401978412 1102 80-81 0.2762004743794435 1102 82-83 0.18283019559051183 1102 84-85 0.2490624817420013 1102 86-87 0.21239344533162097 1102 88-89 0.2510103003317212 1102 90-91 0.10846443819435336 1102 92-93 0.2534194722227845 1102 94-95 0.23308754768760664 1102 96-97 0.12675373272924517 1102 98-99 0.12265918608942883 1102 100-101 0.018342701117674665 1102 102-103 -0.016539264196570258 1102 104-105 0.058136019242500225 1102 106-107 0.05527806424996129 1102 108-109 0.01982652708478483 1102 110-111 0.025856391818976476 1102 112-113 0.033314998911773586 1102 114-115 -0.002371975924052805 1102 116-117 0.024889453890100555 1102 118-119 0.08884367819492667 1102 120-121 -0.0036055667071970277 1102 122-123 0.06808969410544208 1102 124-125 -0.3315326778673118 1103 1 0.46923066910598976 1103 2 0.5212629272473777 1103 3 0.6738363074695854 1103 4 0.6810983402359838 1103 5 0.5975511160952323 1103 6 0.46412608533541544 1103 7 0.5151876034996761 1103 8 0.6495100902733384 1103 9 1.108768837717328 1103 10-11 0.7636737909502216 1103 12-13 0.6583736504564186 1103 14-15 0.8648545838067321 1103 16-17 0.789509906081598 1103 18-19 0.7415828573499965 1103 20-21 0.7360829214697802 1103 22-23 0.7801863640042654 1103 24-25 0.6724540603119848 1103 26-27 0.7689427069535739 1103 28-29 0.6112183557685285 1103 30-31 0.8954750143038197 1103 32-33 0.7598894853739182 1103 34-35 0.7054691509298721 1103 36-37 0.6443409370912008 1103 38-39 0.7773565326186116 1103 40-41 0.7748219390871185 1103 42-43 0.7326589251003455 1103 44-45 0.6000077804634429 1103 46-47 0.5082680549491982 1103 48-49 0.5475215374815221 1103 50-51 0.5139481910264152 1103 52-53 0.4375126824941802 1103 54-55 0.3367703409431613 1103 56-57 0.4126000987711933 1103 58-59 0.5010504708900001 1103 60-61 0.5510584747801701 1103 62-63 0.6317618248132817 1103 64-65 0.6563274259777927 1103 66-67 0.5033392411520978 1103 68-69 0.5326818148599486 1103 70-71 0.6145306966685169 1103 72-73 0.5666084201088779 1103 74-75 0.620831313624393 1103 76-77 0.5725838275198498 1103 78-79 0.46406384492320285 1103 80-81 0.464101415485203 1103 82-83 0.45146459190360844 1103 84-85 0.4944651140371974 1103 86-87 0.45507089726453387 1103 88-89 0.38278328998368494 1103 90-91 0.3734091655804477 1103 92-93 0.4474834598286499 1103 94-95 0.35246989154487096 1103 96-97 0.4418264403287431 1103 98-99 0.44755142095614886 1103 100-101 0.3353781992151639 1103 102-103 0.3118671643656299 1103 104-105 0.23892172105381349 1103 106-107 0.18702672286477195 1103 108-109 0.25285721774445946 1103 110-111 0.29397618930138947 1103 112-113 0.16755646866897322 1103 114-115 0.16191160372065383 1103 116-117 0.13520153096248677 1103 118-119 0.09134013638784921 1103 120-121 0.042432767336791244 1103 122-123 0.002829840712706755 1103 124-125 -0.12248827388081551 1104 1 0.4971692991793333 1104 2 0.5153615965671534 1104 3 0.7542580741601448 1104 4 0.6575243983588024 1104 5 0.6000374366454579 1104 6 0.5665762873495979 1104 7 0.6585523125824437 1104 8 0.7722660415535572 1104 9 1.1851241361129823 1104 10-11 0.8745556588847521 1104 12-13 0.8794667226766748 1104 14-15 0.948387333232219 1104 16-17 0.8001864230728444 1104 18-19 0.8035869725197387 1104 20-21 0.9031392505936964 1104 22-23 0.8234811793896455 1104 24-25 0.9336493036689006 1104 26-27 0.8454589340591809 1104 28-29 0.8614946447392384 1104 30-31 1.0098224277530576 1104 32-33 1.0152430012765166 1104 34-35 0.864671608824267 1104 36-37 0.8198500734506524 1104 38-39 0.976287699043553 1104 40-41 0.8388231189253332 1104 42-43 0.9040603293931042 1104 44-45 0.7181455515371269 1104 46-47 0.7534692565909893 1104 48-49 0.8249466883107957 1104 50-51 0.8090427436481704 1104 52-53 0.6669931002873213 1104 54-55 0.6297457696406354 1104 56-57 0.6763953672939138 1104 58-59 0.751332108868155 1104 60-61 0.7922559188715823 1104 62-63 0.7650958204046816 1104 64-65 0.6655764202370307 1104 66-67 0.6756931763150007 1104 68-69 0.6539030085444537 1104 70-71 0.7570987602412202 1104 72-73 0.6486038647922427 1104 74-75 0.7513431219404154 1104 76-77 0.8365461888468246 1104 78-79 0.6933602059170241 1104 80-81 0.6910665262020217 1104 82-83 0.7414416726802457 1104 84-85 0.8155900765304622 1104 86-87 0.8640549882975108 1104 88-89 0.8353500748888862 1104 90-91 0.734628250227729 1104 92-93 0.7183331012668503 1104 94-95 0.62120056188758 1104 96-97 0.5861191257902938 1104 98-99 0.6558351486132175 1104 100-101 0.6413334784562785 1104 102-103 0.6739416317191136 1104 104-105 0.6216516099320053 1104 106-107 0.5629123784060113 1104 108-109 0.46065510269597 1104 110-111 0.447763872422108 1104 112-113 0.5342226259063594 1104 114-115 0.5389104131844213 1104 116-117 0.5422551397340456 1104 118-119 0.48675028044935686 1104 120-121 0.33145884553112737 1104 122-123 0.4948097936516298 1104 124-125 0.3036195692414587 1105 1 0.22208891587371582 1105 2 0.21501630473219535 1105 3 0.3410129116776339 1105 4 0.13502763216995106 1105 5 0.2346701020977946 1105 6 0.2047875278131528 1105 7 0.3034450028493296 1105 8 0.3676040738124726 1105 9 0.8616976162217469 1105 10-11 0.45940558364915063 1105 12-13 0.37917185189713365 1105 14-15 0.2431448633924589 1105 16-17 0.2815749819810094 1105 18-19 0.36326042359019084 1105 20-21 0.6293779982508312 1105 22-23 0.5828109225326443 1105 24-25 0.5717090169816501 1105 26-27 0.5716546522292418 1105 28-29 0.5469617654593861 1105 30-31 0.7708345446454317 1105 32-33 0.6059747665414221 1105 34-35 0.5118797078712873 1105 36-37 0.5181090951237834 1105 38-39 0.5896347595752189 1105 40-41 0.4871394252530479 1105 42-43 0.518788029295429 1105 44-45 0.5401938079189534 1105 46-47 0.5185509598195139 1105 48-49 0.4152584541036717 1105 50-51 0.394105176002121 1105 52-53 0.34692763211066335 1105 54-55 0.32127529631627993 1105 56-57 0.33948451488768683 1105 58-59 0.40203008410639995 1105 60-61 0.4026035865677855 1105 62-63 0.4296466506361938 1105 64-65 0.45476836342754723 1105 66-67 0.3820520369575391 1105 68-69 0.43459042439936724 1105 70-71 0.5290084247168068 1105 72-73 0.42853839661558624 1105 74-75 0.5014785460316489 1105 76-77 0.4068780174680313 1105 78-79 0.34627776353165274 1105 80-81 0.35200340748814796 1105 82-83 0.3416961538185532 1105 84-85 0.5406730996819178 1105 86-87 0.4681060957128409 1105 88-89 0.4603676298636792 1105 90-91 0.47651785187499485 1105 92-93 0.49631942686543695 1105 94-95 0.4893397345598949 1105 96-97 0.47237793627575897 1105 98-99 0.4117143492678963 1105 100-101 0.36865113110536285 1105 102-103 0.3885959439072799 1105 104-105 0.39118910894206493 1105 106-107 0.2769381433013436 1105 108-109 0.2890345013390849 1105 110-111 0.42184032235743274 1105 112-113 0.48909525334491377 1105 114-115 0.42796561236079 1105 116-117 0.3769202752901286 1105 118-119 0.22912165396114403 1105 120-121 0.2341528082037101 1105 122-123 0.2191755706902505 1105 124-125 0.0752412265366722 1106 1 0.13473611734815805 1106 2 0.22546650466537344 1106 3 0.3862402750386664 1106 4 0.2561989926855759 1106 5 0.20374114034916602 1106 6 0.14118204824298175 1106 7 0.08621431984310846 1106 8 0.26594964025387924 1106 9 0.7459489176735516 1106 10-11 0.2629300121420499 1106 12-13 0.3103664930413359 1106 14-15 0.4685395023810486 1106 16-17 0.32774790531659903 1106 18-19 0.2634593867399815 1106 20-21 0.3792481225219646 1106 22-23 0.5150161154061834 1106 24-25 0.36314648801558036 1106 26-27 0.3409105343602903 1106 28-29 0.2718377637780023 1106 30-31 0.5323433664947288 1106 32-33 0.47998873491856386 1106 34-35 0.4660913460299554 1106 36-37 0.30125866238432764 1106 38-39 0.47911859786107414 1106 40-41 0.4137437946821336 1106 42-43 0.4198270351263602 1106 44-45 0.35661947696757323 1106 46-47 0.4330069367975611 1106 48-49 0.3778299935352365 1106 50-51 0.3208008356738148 1106 52-53 0.25344890262941533 1106 54-55 0.26242402288745126 1106 56-57 0.21991984108593954 1106 58-59 0.28657875247600373 1106 60-61 0.2140107712284376 1106 62-63 0.37015494436012375 1106 64-65 0.2856094839155787 1106 66-67 0.32073743590775194 1106 68-69 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-1.6589540773025035 2216 88-89 -1.9629067571745082 2216 90-91 -1.7997240828380043 2216 92-93 -1.6060583392292926 2216 94-95 -1.6414178049898815 2216 96-97 -1.5694019232090177 2216 98-99 -1.6304105850149497 2216 100-101 -1.7621312614769629 2216 102-103 -2.0265146998818544 2216 104-105 -1.8194379521868385 2216 106-107 -1.5598840141330577 2216 108-109 -1.5363954083296996 2216 110-111 -1.4809972248394274 2216 112-113 -1.7279692211568296 2216 114-115 -1.5487968022069083 2216 116-117 -1.4842850967884438 2216 118-119 -1.3750871115122614 2216 120-121 -1.3058080004757358 2216 122-123 -1.2761023531256441 2216 124-125 -1.2853810551628975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27543.0 3 5459.0 4 3492.0 5 3028.0 6 3306.0 7 3912.0 8 4593.0 9 5011.0 10 5525.0 11 6322.0 12 6713.0 13 7198.0 14 7010.0 15 6823.0 16 6275.0 17 5932.0 18 5647.0 19 5679.0 20 5550.0 21 6189.0 22 7327.0 23 9496.0 24 13329.0 25 18520.0 26 22166.0 27 24976.0 28 30583.0 29 36641.0 30 44816.0 31 54507.0 32 69233.0 33 91716.0 34 125881.0 35 185772.0 36 334465.0 37 569550.0 38 232552.0 39 582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.313153760986786 18.445352876048716 12.528578623189853 24.71291473977465 2 15.686131609121102 19.97153712029735 39.48019054455349 24.862140726028063 3 18.621497624691823 24.522504903113283 29.675403667613597 27.1805938045813 4 12.298428963930865 16.166416408421455 37.87898150218843 33.65617312545925 5 13.089025098225967 38.54012077139144 35.1736569211931 13.197197209189488 6 32.677153955895086 36.85932409996636 17.431066722440917 13.032455221697647 7 28.704864277731108 32.41525688120564 21.38620958519337 17.493669255869886 8 26.294343216722062 35.418715375776095 20.22608763366735 18.060853773834495 9 26.307038158885206 15.890167662685917 19.87332951298552 37.929464665443355 10-11 24.595558355615108 26.54131130649403 28.491132885900676 20.371997451990183 12-13 25.245179462156393 23.89955959671089 28.12378714381807 22.73147379731465 14-15 22.354849563106022 25.577591611955082 25.220868077187262 26.846690747751634 16-17 22.737892919015234 28.723687570182495 26.162711321224236 22.375708189578035 18-19 22.78989854764601 27.811743890655215 27.18597584643684 22.212381715261937 20-21 23.14051876755511 27.10296867963602 27.30416909222168 22.452343460587183 22-23 23.06190985399674 27.025729042796538 27.0598260900821 22.852535013124616 24-25 22.883402750424466 27.142182477338977 27.13369567008086 22.8407191021557 26-27 22.60074044564524 27.494361290747726 26.86441837175843 23.04047989184861 28-29 22.72894848573269 27.564375049765033 26.86914972242655 22.837526742075724 30-31 22.88169568191236 27.230937058468175 26.844286234941112 23.04308102467835 32-33 22.523788463132 27.350193332740975 26.881911782764483 23.244106421362545 34-35 23.287754315073023 27.258457618002318 26.61052589542991 22.84326217149475 36-37 23.229912569478937 26.902269000476714 26.71819817751166 23.149620252532692 38-39 22.801703977361502 27.19090826046654 26.7932170359799 23.214170726192062 40-41 23.14424300389409 26.944435986148807 26.851963242521187 23.059357767435916 42-43 22.795403600175415 26.880188252818467 26.913758474609057 23.410649672397067 44-45 22.82776842375344 27.18161610584951 26.918924153532902 23.071691316864147 46-47 23.21236030438021 27.427938325269906 26.401923042364437 22.957778327985448 48-49 22.883884997244344 27.242715480402886 26.666997811449157 23.206401710903616 50-51 23.003800383283103 27.301583101997334 26.691739645041306 23.00287686967825 52-53 23.145188022172878 27.068648261154333 26.70405078355884 23.082112933113947 54-55 22.74638688808732 27.173576889063277 26.87647064843443 23.203565574414966 56-57 22.793422958540415 27.426845976987398 26.838913005439764 22.940818059032424 58-59 22.856446093744346 27.04030348360661 26.743818890296957 23.35943153235209 60-61 22.60466516433012 27.118938526345637 27.13621173559809 23.14018457372615 62-63 22.62738101864876 27.15226812237604 27.20576139802417 23.014589460951033 64-65 22.545356245338574 27.189060985192263 26.897568919227894 23.36801385024127 66-67 22.509997254187365 27.275679588627344 26.807144105239512 23.407179051945786 68-69 22.75490326271783 26.924012033624656 27.22045935855948 23.100625345098035 70-71 22.75213269030227 26.88238092551945 26.920170066108533 23.445316318069747 72-73 22.730175101918963 27.063565680942475 26.71859383284192 23.48766538429664 74-75 22.76112789938948 27.324391640552335 26.725704937118426 23.188775522939757 76-77 22.937263455944993 27.0643963928615 26.814395706457862 23.183944444735648 78-79 22.928423232371642 27.055177481708682 26.87962774369788 23.136771542221794 80-81 22.99104785949161 27.247202440489843 26.737477350058576 23.024272349959972 82-83 22.96598737270103 27.244800450532765 26.78588011155479 23.00333206521142 84-85 22.930337132502807 27.202100023189917 26.83225949944222 23.035303344865056 86-87 22.98369713501031 27.21848794665379 26.7540825809795 23.0437323373564 88-89 22.912702305663622 27.234389372953853 26.761099898776962 23.091808422605563 90-91 22.856072360709227 27.314864738032906 26.703030738723843 23.12603216253402 92-93 23.01351836403783 26.84298099920774 26.970431729990164 23.173068906764275 94-95 22.82379546265766 26.92340333124496 27.04166677066927 23.211134435428114 96-97 23.038513966014694 26.75409814787404 27.01047185892282 23.196916027188443 98-99 22.81054615007714 26.886447599599933 26.785707599904452 23.51729865041847 100-101 22.729455708379863 26.80244461207359 27.078317147579824 23.38978253196672 102-103 22.628552029873994 26.548939632965237 27.19786528745732 23.624643049703455 104-105 22.492255166280227 26.90731585068115 26.93681997913744 23.663609003901183 106-107 22.75720433725389 26.850973166473892 26.733161841311144 23.658660654961082 108-109 22.749249652125624 26.746529056090452 26.743259348928923 23.760961942855 110-111 22.71082495391685 26.973613106210276 26.876367362271598 23.43919457760128 112-113 22.765451537440832 27.150605001961925 26.933270565024607 23.150672895572637 114-115 22.702201982068427 27.30491213688663 26.66918448233427 23.32370139871067 116-117 22.707716685279475 27.195183867413576 26.85351362712115 23.243585820185803 118-119 22.566790372691393 27.432921977658875 26.555090285721146 23.445197363928582 120-121 22.55079164565431 27.539831934451442 26.6968079529057 23.21256846698855 122-123 22.564954721246 27.591641636631042 26.639980167373984 23.203423474748977 124-125 22.56643511123242 27.649755289428445 26.59275772790306 23.191051871436073 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 316.0 1 283.5 2 223.0 3 187.5 4 154.0 5 124.0 6 112.0 7 108.5 8 111.0 9 108.5 10 105.5 11 102.0 12 110.0 13 125.0 14 137.5 15 149.5 16 180.5 17 234.5 18 315.0 19 415.5 20 559.5 21 791.5 22 1177.0 23 1749.0 24 2543.0 25 3927.0 26 6014.0 27 8735.0 28 11762.5 29 15154.5 30 19470.0 31 24740.5 32 31752.5 33 36579.5 34 38555.0 35 42402.0 36 48065.5 37 53276.5 38 57645.5 39 61678.5 40 64856.5 41 68019.0 42 69995.0 43 71465.5 44 73711.5 45 76683.0 46 77832.0 47 78103.0 48 77163.0 49 74882.0 50 74422.0 51 73288.5 52 71630.5 53 71880.0 54 73511.0 55 69146.5 56 61096.0 57 55152.0 58 50880.0 59 44346.0 60 34431.0 61 27901.5 62 23733.5 63 17912.5 64 13175.5 65 10314.0 66 7518.5 67 5888.0 68 4508.0 69 3023.0 70 2050.0 71 1366.0 72 1013.5 73 730.5 74 535.5 75 410.0 76 255.5 77 140.0 78 89.0 79 61.0 80 41.5 81 26.0 82 17.5 83 13.0 84 11.0 85 9.5 86 6.5 87 5.0 88 6.5 89 8.5 90 6.5 91 2.0 92 2.0 93 4.5 94 5.0 95 3.5 96 2.5 97 2.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09099898718077351 2 3.993372997510631E-4 3 0.0 4 0.002745443935788559 5 0.0014975148740664866 6 2.495858123444145E-4 7 0.0 8 0.0012479290617220722 9 5.490887871577117E-4 10-11 0.009334509381681101 12-13 0.00207156224245864 14-15 0.004342793134792811 16-17 0.002970071166898532 18-19 0.013003420823143992 20-21 0.017021752401889065 22-23 0.010632355605872055 24-25 0.010482604118465407 26-27 0.010033349656245461 28-29 0.0077621187639112885 30-31 0.01742108970164013 32-33 0.001846935011348667 34-35 0.001073218993080982 36-37 6.988402745643604E-4 38-39 2.995029748132973E-4 40-41 0.0012978462241909551 42-43 0.0027704025170230003 44-45 0.0010981775743154234 46-47 0.0017471006864109011 48-49 0.008336166132303442 50-51 0.004991716246888289 52-53 0.0075374915328013155 54-55 0.008161456063662352 56-57 0.009958473912542136 58-59 0.008960130663164478 60-61 0.010832024255747587 62-63 0.01315317231055064 64-65 0.008835337756992271 66-67 0.013427716704129497 68-69 0.013228048054253965 70-71 0.004891881921950523 72-73 0.012828710754502901 74-75 0.013377799541660615 76-77 0.0062146867273759196 78-79 0.00913484073180557 80-81 0.013901929747583884 82-83 0.017820427001391193 84-85 0.01465068718461713 86-87 0.016347870708559146 88-89 0.015798781921401432 90-91 0.012129870479938541 92-93 0.009584095194025513 94-95 0.008186414644896795 96-97 0.009409385125384424 98-99 0.0071131956518158115 100-101 0.010981775743154235 102-103 0.01193020183006301 104-105 0.010457645537230966 106-107 0.0057903908463904155 108-109 0.004068248741213956 110-111 0.007312864301691343 112-113 0.009234675056743335 114-115 0.012978462241909551 116-117 0.01088194141821647 118-119 0.21761386978309494 120-121 0.026406178946039045 122-123 0.027878735238871095 124-125 0.23628288854645715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2003319.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.41470633282667 #Duplication Level Percentage of deduplicated Percentage of total 1 85.5598590165776 62.813519235800975 2 8.95035424386169 13.1417525677575 3 2.6464967600636036 5.828753473525401 4 1.0408002392392066 3.0564017565952843 5 0.5428407969638795 1.9926248847290404 6 0.3387359696917908 1.492092104357286 7 0.18719442377039613 0.9619976553772438 8 0.13166680454235985 0.7733023831407231 9 0.09991009743598354 0.6601383416652148 >10 0.45524279216083846 5.950149694570686 >50 0.032710114764909155 1.6603619828559815 >100 0.014188740927682143 1.6689059196246525 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 2.4958581234441444E-5 0.0 0.0 0.0 16-17 0.0 4.991716246888289E-5 0.0 2.4958581234441444E-5 0.0 18-19 0.0 4.991716246888289E-5 0.0 4.991716246888289E-5 0.0 20-21 0.0 4.991716246888289E-5 0.0 4.991716246888289E-5 0.0 22-23 0.0 4.991716246888289E-5 0.0 1.9966864987553155E-4 2.4958581234441444E-5 24-25 0.0 4.991716246888289E-5 0.0 4.242958809855046E-4 4.991716246888289E-5 26-27 0.0 4.991716246888289E-5 0.0 7.487574370332434E-4 4.991716246888289E-5 28-29 0.0 4.991716246888289E-5 0.0 0.0028203196794918833 4.991716246888289E-5 30-31 0.0 4.991716246888289E-5 0.0 0.010832024255747587 4.991716246888289E-5 32-33 0.0 4.991716246888289E-5 0.0 0.028078403888746627 7.487574370332434E-5 34-35 0.0 4.991716246888289E-5 0.0 0.057055316701933145 9.983432493776577E-5 36-37 4.991716246888289E-5 4.991716246888289E-5 0.0 0.10612388740884501 9.983432493776577E-5 38-39 4.991716246888289E-5 4.991716246888289E-5 0.0 0.1806002938124183 9.983432493776577E-5 40-41 4.991716246888289E-5 4.991716246888289E-5 0.0 0.2625143574238551 9.983432493776577E-5 42-43 4.991716246888289E-5 4.991716246888289E-5 0.0 0.340360172294078 9.983432493776577E-5 44-45 4.991716246888289E-5 4.991716246888289E-5 0.0 0.4355522011222377 9.983432493776577E-5 46-47 4.991716246888289E-5 4.991716246888289E-5 0.0 0.5478158995147553 9.983432493776577E-5 48-49 4.991716246888289E-5 4.991716246888289E-5 0.0 0.6674174207901986 9.983432493776577E-5 50-51 4.991716246888289E-5 4.991716246888289E-5 0.0 0.8005964102571782 9.983432493776577E-5 52-53 4.991716246888289E-5 4.991716246888289E-5 0.0 0.9303810326762737 1.4975148740664868E-4 54-55 4.991716246888289E-5 4.991716246888289E-5 0.0 1.0661307560104007 1.9966864987553155E-4 56-57 4.991716246888289E-5 4.991716246888289E-5 0.0 1.2321552383819052 1.9966864987553155E-4 58-59 4.991716246888289E-5 4.991716246888289E-5 0.0 1.4125309049632135 1.9966864987553155E-4 60-61 4.991716246888289E-5 4.991716246888289E-5 0.0 1.6082810575849378 1.9966864987553155E-4 62-63 4.991716246888289E-5 4.991716246888289E-5 0.0 1.7977915648980516 2.495858123444144E-4 64-65 4.991716246888289E-5 4.991716246888289E-5 0.0 1.9920192440644748 2.495858123444144E-4 66-67 4.991716246888289E-5 4.991716246888289E-5 0.0 2.220215552290973 2.495858123444144E-4 68-69 4.991716246888289E-5 4.991716246888289E-5 0.0 2.475442003994371 2.495858123444144E-4 70-71 4.991716246888289E-5 4.991716246888289E-5 0.0 2.7533807646211113 2.495858123444144E-4 72-73 4.991716246888289E-5 4.991716246888289E-5 0.0 3.031419359572789 2.9950297481329735E-4 74-75 4.991716246888289E-5 4.991716246888289E-5 0.0 3.3079604396504 2.9950297481329735E-4 76-77 4.991716246888289E-5 4.991716246888289E-5 0.0 3.606365236889382 2.9950297481329735E-4 78-79 4.991716246888289E-5 4.991716246888289E-5 0.0 3.951642249686645 2.9950297481329735E-4 80-81 4.991716246888289E-5 4.991716246888289E-5 0.0 4.316337038684304 2.9950297481329735E-4 82-83 4.991716246888289E-5 4.991716246888289E-5 0.0 4.699950432257669 3.494201372821802E-4 84-85 4.991716246888289E-5 4.991716246888289E-5 0.0 5.084761837730287 3.494201372821802E-4 86-87 4.991716246888289E-5 4.991716246888289E-5 0.0 5.4807297290146995 3.494201372821802E-4 88-89 4.991716246888289E-5 4.991716246888289E-5 0.0 5.912113847070786 3.494201372821802E-4 90-91 4.991716246888289E-5 4.991716246888289E-5 0.0 6.380586416841252 3.494201372821802E-4 92-93 4.991716246888289E-5 4.991716246888289E-5 0.0 6.864558265558307 3.494201372821802E-4 94-95 4.991716246888289E-5 4.991716246888289E-5 0.0 7.3493787060373315 3.494201372821802E-4 96-97 4.991716246888289E-5 4.991716246888289E-5 0.0 7.838566898232383 3.494201372821802E-4 98-99 4.991716246888289E-5 4.991716246888289E-5 0.0 8.359602240082584 3.494201372821802E-4 100-101 4.991716246888289E-5 4.991716246888289E-5 0.0 8.938816034790266 3.494201372821802E-4 102-103 4.991716246888289E-5 4.991716246888289E-5 0.0 9.539194706384755 4.49254462219946E-4 104-105 9.983432493776577E-5 4.991716246888289E-5 0.0 10.1423687390775 4.49254462219946E-4 106-107 9.983432493776577E-5 4.991716246888289E-5 0.0 10.74292212074063 4.49254462219946E-4 108-109 1.2479290617220724E-4 4.991716246888289E-5 0.0 11.365239385240194 4.7421304345438746E-4 110-111 1.4975148740664868E-4 4.991716246888289E-5 0.0 12.033155977655081 5.740473683921532E-4 112-113 1.4975148740664868E-4 4.991716246888289E-5 0.0 12.729200891121184 5.990059496265947E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2125 0.0 44.25508 1 GGTATCA 1380 0.0 39.680187 1 ATCAACG 2360 0.0 39.06244 3 TCAACGC 2515 0.0 36.893337 4 CAACGCA 2520 0.0 36.821976 5 GTACATG 7045 0.0 35.65303 1 AACGCAG 2645 0.0 35.081806 6 TACATGG 7105 0.0 34.320965 2 ACATGGG 7085 0.0 33.746277 3 TATCAAC 2845 0.0 32.821396 2 CATGGGG 4170 0.0 28.954851 4 CGCAGAG 3315 0.0 27.453072 8 ACGCAGA 3375 0.0 26.788773 7 GTACCGT 160 8.766539E-6 26.023222 6 CATGGGA 3650 0.0 24.769207 4 ATGGGGA 2385 0.0 23.443502 5 GTATTAC 615 0.0 22.259619 1 GAGTACA 5440 0.0 22.210693 1 AGTACCG 270 4.561116E-8 22.030241 5 GGACCGA 485 0.0 20.849237 6 >>END_MODULE