FastQCFastQC Report
Thu 26 May 2016
SRR1364429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003319
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22050.063.2327351
GTATCAA39200.051.6806031
ATCAACG48550.040.7305953
TCAACGC49750.039.7471544
CAACGCA50400.039.075355
TATCAAC52600.037.707732
AACGCAG54500.036.4623456
GTACATG68450.030.9892651
TACATGG67800.030.659682
ACGCAGA64600.030.5773757
CGCAGAG64450.030.5539388
ACATGGG69850.028.3955123
CATGGGG40600.025.5260854
GCAGAGT79000.024.5500649
TAAGGTG10750.024.3528445
GAGTACA53850.023.015071
AGAGTAC72650.022.88802510-11
GTAAGGT11300.022.6647554
GAGTACT44850.022.5506712-13
CATGGGA36550.022.3251424
Produced by FastQC (version 0.11.5)