FastQCFastQC Report
Thu 26 May 2016
SRR1364428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132757
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.050.7738571
GTATCAA26100.046.066881
ATCAACG28550.039.5784453
GTACATG81150.037.994361
CAACGCA29950.037.9313855
TCAACGC30600.037.123914
ACATGGG81400.036.3114173
AACGCAG31750.035.7809456
TACATGG85800.035.489012
TATCAAC33600.034.8688932
CATGGGG44400.029.6040464
ACGCAGA40450.028.0851677
CGCAGAG41250.027.3962928
CATGGGA46400.026.4052944
GAGTACA62750.023.0499741
TAGGACG1552.1128514E-423.022974
GTATAGG8800.022.320771
AGTACAT61650.021.7050442
ATGGGGA28000.020.8175755
GTATAGT8050.019.9639191
Produced by FastQC (version 0.11.5)