FastQCFastQC Report
Thu 26 May 2016
SRR1364428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132757
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.10695076841853057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.065.924431
GTATCAA47150.048.1321831
ATCAACG55750.040.2685933
CAACGCA58400.038.3097085
TCAACGC59550.037.598984
TATCAAC60850.036.8944442
AACGCAG61000.036.8710566
GTACATG74450.034.402981
ACATGGG76650.031.4639233
ACGCAGA72000.031.403267
TACATGG79700.031.2318822
CGCAGAG73500.030.5159388
CATGGGG42800.026.9915584
TATTCCG2507.021299E-1026.1810175
GCAGAGT89250.024.9307949
GAGTACT51150.024.71644612-13
GTGGTAT17000.024.526831
GTACTTT51350.024.3305314-15
CAGAGTA83850.023.911110-11
TGGTATC16700.023.891232
Produced by FastQC (version 0.11.5)