FastQCFastQC Report
Thu 26 May 2016
SRR1364427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446552
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29550.052.3741
GGTATCA18250.052.186361
TCAACGC33950.043.7980424
ATCAACG33900.043.685683
CAACGCA34400.043.39955
AACGCAG35300.041.4505126
TATCAAC37300.040.9792252
GTACATG88650.038.4075971
TACATGG90550.036.782312
ACATGGG91900.034.4946023
ACGCAGA44750.032.6972737
CGCAGAG45950.031.5844828
CATGGGA64300.029.877624
CATGGGG33450.029.1609844
GCAGAGT53300.026.5594589
GAGTACA69550.026.1037311
ATGGGGA18600.025.902495
GTATAGA5350.025.5902141
ATGGGAG27800.025.2468195
AGTACAT68500.025.1794852
Produced by FastQC (version 0.11.5)