##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364427_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1446552 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.460627063527618 31.0 31.0 34.0 26.0 34.0 2 30.43684568546447 31.0 31.0 34.0 26.0 34.0 3 30.356415808073265 31.0 31.0 34.0 26.0 34.0 4 33.8229244437808 37.0 35.0 37.0 28.0 37.0 5 34.060395340091475 37.0 35.0 37.0 30.0 37.0 6 34.12703518435563 37.0 35.0 37.0 30.0 37.0 7 34.00075144204978 37.0 35.0 37.0 30.0 37.0 8 33.831175789048714 37.0 35.0 37.0 29.0 37.0 9 34.81894187004684 39.0 35.0 39.0 27.0 39.0 10-11 35.07680124876258 39.0 35.0 39.0 27.5 39.0 12-13 35.131674492171726 39.0 35.0 39.0 27.5 39.0 14-15 36.26619402551723 40.0 36.0 41.0 27.0 41.0 16-17 36.439804790978826 40.0 36.0 41.0 28.5 41.0 18-19 36.43077020390557 40.0 36.0 41.0 28.0 41.0 20-21 36.39827845801602 40.0 36.0 41.0 27.5 41.0 22-23 36.341430173267185 40.0 36.0 41.0 27.0 41.0 24-25 36.43040485236618 40.0 36.0 41.0 28.0 41.0 26-27 36.47165915915916 40.0 36.0 41.0 29.0 41.0 28-29 36.43285066834791 40.0 36.0 41.0 29.0 41.0 30-31 36.208375848223916 40.0 36.0 41.0 28.0 41.0 32-33 36.28059551263971 40.0 36.0 41.0 29.0 41.0 34-35 36.22140510676422 40.0 36.0 41.0 29.0 41.0 36-37 36.10147647647648 40.0 35.0 41.0 28.0 41.0 38-39 35.963065275219975 40.0 35.0 41.0 27.5 41.0 40-41 35.91944776267981 40.0 35.0 41.0 27.5 41.0 42-43 35.7785340589208 39.5 35.0 41.0 27.0 41.0 44-45 35.76250317997555 39.0 35.0 41.0 27.0 41.0 46-47 35.60905034869123 39.0 35.0 41.0 27.0 41.0 48-49 35.45183304851813 39.0 35.0 41.0 27.0 41.0 50-51 35.31744382504051 39.0 34.5 41.0 26.5 41.0 52-53 35.18465046538251 39.0 34.0 40.5 27.0 41.0 54-55 35.05062659344427 38.5 34.0 40.0 26.5 41.0 56-57 35.00519234704318 38.0 34.0 40.0 26.5 41.0 58-59 34.97490549942208 38.0 34.0 40.0 27.0 41.0 60-61 34.782793843567326 38.0 34.0 40.0 26.5 41.0 62-63 34.52397286789552 37.5 34.0 40.0 26.0 41.0 64-65 34.21094920887738 37.0 33.5 40.0 26.0 41.0 66-67 33.89567018676135 36.5 33.0 40.0 26.0 41.0 68-69 33.59409547669216 36.0 33.0 39.0 26.0 41.0 70-71 33.240712743129876 35.5 33.0 39.0 25.0 41.0 72-73 32.90616376044552 35.0 33.0 38.5 25.0 40.0 74-75 32.558594160458796 35.0 32.0 37.0 24.0 39.5 76-77 32.21800287856918 35.0 32.0 37.0 23.5 39.0 78-79 31.902214714714717 35.0 32.0 36.5 23.0 39.0 80-81 31.58309448951714 35.0 32.0 36.0 22.0 38.0 82-83 31.302557045996274 35.0 32.0 36.0 20.5 37.0 84-85 30.969803366902816 35.0 31.5 35.0 19.5 37.0 86-87 30.776475370398025 35.0 31.0 35.0 19.5 36.0 88-89 30.636321058627686 35.0 31.0 35.0 19.0 36.0 90-91 30.5070191047401 35.0 31.0 35.0 18.5 36.0 92-93 30.276949601535236 34.5 31.0 35.0 17.0 35.5 94-95 30.198101070683943 34.0 31.0 35.0 16.0 35.0 96-97 30.122251395041452 34.0 31.0 35.0 14.0 35.0 98-99 29.967487169489935 34.0 31.0 35.0 8.5 35.0 100-101 29.832398351390065 34.0 30.5 35.0 7.5 35.0 102-103 28.820211786372006 33.5 29.0 34.5 7.0 35.0 104-105 29.75313262157185 34.0 30.5 35.0 7.0 35.0 106-107 29.992807724851925 35.0 31.0 35.0 6.0 35.0 108-109 29.97309913504665 35.0 31.0 35.0 2.0 35.0 110-111 29.863313589832927 35.0 31.0 35.0 2.0 35.0 112-113 29.750479761529483 35.0 31.0 35.0 2.0 35.0 114-115 29.64173946045493 35.0 31.0 35.0 2.0 35.0 116-117 29.514213453785274 35.0 31.0 35.0 2.0 35.0 118-119 29.245349631399357 34.5 30.5 35.0 2.0 35.0 120-121 29.108654925643876 34.0 30.0 35.0 2.0 35.0 122-123 28.86359252899308 34.0 30.0 35.0 2.0 35.0 124-125 27.6980184604494 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6680796250009102 1101 2 0.5966872593756136 1101 3 0.7411567510021868 1101 4 0.6513906863714993 1101 5 0.41558654310397714 1101 6 0.49513227721598696 1101 7 0.38544704852180445 1101 8 0.560901071227569 1101 9 0.9182404637793979 1101 10-11 0.6876875250365586 1101 12-13 0.6006925814634911 1101 14-15 0.9090526149407125 1101 16-17 0.6387607674886056 1101 18-19 0.7129476276765487 1101 20-21 0.782712369364063 1101 22-23 0.7286835023618323 1101 24-25 0.7983219536826454 1101 26-27 0.7528822195896936 1101 28-29 0.8474056587095831 1101 30-31 1.0162427678475439 1101 32-33 0.8470632633182618 1101 34-35 0.8589490052463731 1101 36-37 0.8243776868137118 1101 38-39 0.9067622042318106 1101 40-41 0.7772339275903519 1101 42-43 0.9241071643661698 1101 44-45 0.7130143776813824 1101 46-47 0.7339749356992229 1101 48-49 0.6426645869741989 1101 50-51 0.7113842563355632 1101 52-53 0.7432793106428903 1101 54-55 0.6439408805852693 1101 56-57 0.5746517553682366 1101 58-59 0.5749675215433072 1101 60-61 0.5816623843873856 1101 62-63 0.6567228746528784 1101 64-65 0.5506382014262883 1101 66-67 0.6343344541012215 1101 68-69 0.514765174003756 1101 70-71 0.45832464236693937 1101 72-73 0.484498036826551 1101 74-75 0.46913887700059576 1101 76-77 0.47799878711896326 1101 78-79 0.4368243485383161 1101 80-81 0.48614232407263813 1101 82-83 0.5946557644338952 1101 84-85 0.5898195167433009 1101 86-87 0.5118650944130394 1101 88-89 0.41880090126677416 1101 90-91 0.3941117390288653 1101 92-93 0.5548371438735629 1101 94-95 0.4759583501317053 1101 96-97 0.39561900416691387 1101 98-99 0.4019348265573299 1101 100-101 0.45651998638385294 1101 102-103 0.40479949190986275 1101 104-105 0.419583593524397 1101 106-107 0.2945900597438431 1101 108-109 0.29986142253449444 1101 110-111 0.2350428954081707 1101 112-113 0.21669879371940226 1101 114-115 0.2657527882276369 1101 116-117 0.29513678291298007 1101 118-119 0.29710437226734854 1101 120-121 0.12286052353938004 1101 122-123 0.1063278184808567 1101 124-125 -0.16775037201852783 1102 1 0.45668081771133373 1102 2 0.42360194403495655 1102 3 0.6356479104424437 1102 4 0.5053719286943235 1102 5 0.5257146823420698 1102 6 0.32055710502862667 1102 7 0.4575133050791109 1102 8 0.44648031288548395 1102 9 0.795142073006275 1102 10-11 0.6410428789930478 1102 12-13 0.5271154318747691 1102 14-15 0.7736247513178895 1102 16-17 0.5807464760821759 1102 18-19 0.4845728255610524 1102 20-21 0.6945227021730034 1102 22-23 0.75040645663411 1102 24-25 0.6236185020159581 1102 26-27 0.5431815686228916 1102 28-29 0.6144478556462261 1102 30-31 0.7769310058154701 1102 32-33 0.6991172198946316 1102 34-35 0.5648183895131211 1102 36-37 0.44367657140095673 1102 38-39 0.600497009713095 1102 40-41 0.6093532118321079 1102 42-43 0.6761833533024557 1102 44-45 0.5101818347861311 1102 46-47 0.5335807340966809 1102 48-49 0.46142321284111887 1102 50-51 0.3688481421561036 1102 52-53 0.2823199946241317 1102 54-55 0.25242221653974184 1102 56-57 0.2852652117618746 1102 58-59 0.3710448093108809 1102 60-61 0.37980369310205475 1102 62-63 0.3966213812487567 1102 64-65 0.3973188564683241 1102 66-67 0.2900139764890852 1102 68-69 0.30653705596684233 1102 70-71 0.3423513310982287 1102 72-73 0.3118911915695435 1102 74-75 0.31141183263405026 1102 76-77 0.44077711440404954 1102 78-79 0.29791891485056254 1102 80-81 0.45088258493782973 1102 82-83 0.3570285470756609 1102 84-85 0.49651867537840744 1102 86-87 0.39272872466455766 1102 88-89 0.3552173830762513 1102 90-91 0.29290129221205774 1102 92-93 0.34326922215786837 1102 94-95 0.36642437999318744 1102 96-97 0.25800564594213427 1102 98-99 0.20638204099524415 1102 100-101 0.11580948305807226 1102 102-103 0.09299600617280745 1102 104-105 0.1546844063460746 1102 106-107 0.04834460624082482 1102 108-109 0.06541520628416464 1102 110-111 0.08933122478038058 1102 112-113 0.08028697286850317 1102 114-115 0.10910828242536041 1102 116-117 0.054257000171396896 1102 118-119 0.23177608816831352 1102 120-121 0.22813590369623427 1102 122-123 0.2463407454226001 1102 124-125 0.10919471199009223 1103 1 0.655009706630544 1103 2 0.7494643408803476 1103 3 0.9032111633954116 1103 4 0.7870312372759471 1103 5 0.5906356763785681 1103 6 0.5356917635279146 1103 7 0.5417800790825709 1103 8 0.7401553160620935 1103 9 1.1998091768657133 1103 10-11 0.9249300798478828 1103 12-13 0.7042524815740379 1103 14-15 0.964331903512992 1103 16-17 0.8141878000087601 1103 18-19 0.8574132624240818 1103 20-21 0.9513777881206096 1103 22-23 0.9562952556019013 1103 24-25 0.7863802100695381 1103 26-27 0.7878877128299919 1103 28-29 0.8776909163480795 1103 30-31 1.127739826648579 1103 32-33 0.9451566883387272 1103 34-35 0.9308834755204174 1103 36-37 0.8789561942667703 1103 38-39 1.038890565071 1103 40-41 1.0843959148773124 1103 42-43 1.0769052756771416 1103 44-45 0.8234017424639788 1103 46-47 0.7924575523339286 1103 48-49 0.8060587467208578 1103 50-51 0.7427575409193921 1103 52-53 0.8406564477172935 1103 54-55 0.8161422807030405 1103 56-57 0.7821753703536913 1103 58-59 0.8144243662566666 1103 60-61 0.7933921730442037 1103 62-63 0.7172534545402272 1103 64-65 0.789877525719497 1103 66-67 0.5533374118691228 1103 68-69 0.629962629014841 1103 70-71 0.6513030662105734 1103 72-73 0.5123759127056573 1103 74-75 0.620658843420884 1103 76-77 0.5989832624486624 1103 78-79 0.582406687239704 1103 80-81 0.6529613683253004 1103 82-83 0.5952454486979093 1103 84-85 0.7007955036784814 1103 86-87 0.658418202958277 1103 88-89 0.6726391595399832 1103 90-91 0.5988235202237178 1103 92-93 0.5633954253262949 1103 94-95 0.5379072736180497 1103 96-97 0.5998048277833412 1103 98-99 0.6641535688304643 1103 100-101 0.5266065951980181 1103 102-103 0.41693793855915473 1103 104-105 0.37823602053397565 1103 106-107 0.4589632738034801 1103 108-109 0.4513070411549229 1103 110-111 0.5836988146401225 1103 112-113 0.5545625987463367 1103 114-115 0.5231372241317622 1103 116-117 0.45746861881081813 1103 118-119 0.42394405546924574 1103 120-121 0.3546119622755164 1103 122-123 0.39369226119948664 1103 124-125 0.16520541610638517 1104 1 0.41912198352173036 1104 2 0.4409909268445169 1104 3 0.5415723475309413 1104 4 0.3887769487049155 1104 5 0.5132656170569234 1104 6 0.4316896193511752 1104 7 0.4755633936630588 1104 8 0.631825224229722 1104 9 1.105702530178874 1104 10-11 0.7415816485167994 1104 12-13 0.7206105312434019 1104 14-15 0.7676376253495718 1104 16-17 0.6224654186846763 1104 18-19 0.6658070530135447 1104 20-21 0.7617208302931857 1104 22-23 0.8463046015670841 1104 24-25 0.8802680010540769 1104 26-27 0.8926253949087979 1104 28-29 0.7768308525971079 1104 30-31 0.8843731558919643 1104 32-33 0.8271212380659563 1104 34-35 0.7914029945774033 1104 36-37 0.7023596571470065 1104 38-39 0.8975417612936241 1104 40-41 0.8714717242300623 1104 42-43 0.8574463061176658 1104 44-45 0.6357773580228283 1104 46-47 0.6463324295267299 1104 48-49 0.7003964754424672 1104 50-51 0.6651616642826568 1104 52-53 0.5781327238461884 1104 54-55 0.49381221149590715 1104 56-57 0.532652893481476 1104 58-59 0.5831180370193678 1104 60-61 0.674533729608612 1104 62-63 0.6516996451892183 1104 64-65 0.5315000949200979 1104 66-67 0.5277621868567408 1104 68-69 0.5588900011191384 1104 70-71 0.5632871780697712 1104 72-73 0.5292826244835709 1104 74-75 0.5108304819843852 1104 76-77 0.563984841864567 1104 78-79 0.43877795527136954 1104 80-81 0.5420820209068822 1104 82-83 0.511331690897638 1104 84-85 0.5654513084887824 1104 86-87 0.6361216800228071 1104 88-89 0.5921213634821711 1104 90-91 0.5391936082620106 1104 92-93 0.586094851602379 1104 94-95 0.5255582427180592 1104 96-97 0.3974379229424976 1104 98-99 0.554505556675494 1104 100-101 0.5273273396728868 1104 102-103 0.5034594730030406 1104 104-105 0.5530029587905609 1104 106-107 0.5473250670801306 1104 108-109 0.49576063721952224 1104 110-111 0.5908773251856587 1104 112-113 0.6303869624766811 1104 114-115 0.6165048521101362 1104 116-117 0.633484296362834 1104 118-119 0.6382858045222513 1104 120-121 0.5054368263717812 1104 122-123 0.4144126314876715 1104 124-125 0.1863417427591827 1105 1 0.20542340020406513 1105 2 0.2578154231040486 1105 3 0.35843549759641036 1105 4 0.07230115046313301 1105 5 0.1979766940984362 1105 6 0.1821630267533365 1105 7 0.2227321040988528 1105 8 0.25490626620612744 1105 9 0.6920868694469888 1105 10-11 0.32374661970546725 1105 12-13 0.4170223187825499 1105 14-15 -0.014208024713859402 1105 16-17 0.09456550844287648 1105 18-19 0.1290055781470656 1105 20-21 0.4644557701600718 1105 22-23 0.4122518598729741 1105 24-25 0.3861759301296388 1105 26-27 0.3125926897201552 1105 28-29 0.32643365422496373 1105 30-31 0.47661476740575637 1105 32-33 0.2756396580285738 1105 34-35 0.2642914185355991 1105 36-37 0.23637154787147807 1105 38-39 0.3105192273454094 1105 40-41 0.26058941652460277 1105 42-43 0.29632324623356254 1105 44-45 0.15970426733775156 1105 46-47 0.34075903896948745 1105 48-49 0.2626256444995647 1105 50-51 0.08265796368934275 1105 52-53 0.036351825010925154 1105 54-55 0.0631131960693736 1105 56-57 0.08897270937543311 1105 58-59 0.28404296156307396 1105 60-61 0.21072081240181006 1105 62-63 0.26405384913645946 1105 64-65 0.346962146240962 1105 66-67 0.19963063859685803 1105 68-69 0.20583061341170605 1105 70-71 0.3400681623502848 1105 72-73 0.24576163709448196 1105 74-75 0.26306894423019855 1105 76-77 0.2837506486264587 1105 78-79 0.23206289403967162 1105 80-81 0.21883672059936288 1105 82-83 0.2166513497350806 1105 84-85 0.3372844021685175 1105 86-87 0.3150800535357554 1105 88-89 0.3160127519838447 1105 90-91 0.21154177407788666 1105 92-93 0.200213906952186 1105 94-95 0.10541883674974528 1105 96-97 0.11538435609135078 1105 98-99 0.11899780434954721 1105 100-101 -0.09776308988888971 1105 102-103 -0.11535011997645483 1105 104-105 -0.051774895752320305 1105 106-107 -0.05626387776862174 1105 108-109 0.03845539340352033 1105 110-111 0.1530338657758179 1105 112-113 -0.024574111106332452 1105 114-115 -0.06339097791727966 1105 116-117 -0.03809689656685222 1105 118-119 -0.18169935241627044 1105 120-121 -0.18619084063910663 1105 122-123 -0.02266426191815185 1105 124-125 -0.20056820689321597 1106 1 0.42555084994076964 1106 2 0.4239300029390449 1106 3 0.4841651602559942 1106 4 0.30166556363101193 1106 5 0.28227536002890474 1106 6 0.2961654551385351 1106 7 0.12170368412625265 1106 8 0.496519603893951 1106 9 0.9464634958732034 1106 10-11 0.426918191789909 1106 12-13 0.5491864503225301 1106 14-15 0.784625633213885 1106 16-17 0.5671611545464188 1106 18-19 0.47435015783168666 1106 20-21 0.7427233421180475 1106 22-23 0.7283506501916648 1106 24-25 0.7049364781627361 1106 26-27 0.7102500190439685 1106 28-29 0.7094417248077676 1106 30-31 0.8698770588405154 1106 32-33 0.8523383420042023 1106 34-35 0.6638840721498553 1106 36-37 0.7235654597116081 1106 38-39 0.8571004211253808 1106 40-41 0.8550012772853606 1106 42-43 0.8234553803862283 1106 44-45 0.6606792073125263 1106 46-47 0.7958289003494841 1106 48-49 0.6362959223834821 1106 50-51 0.5965263257786191 1106 52-53 0.5853731641855475 1106 54-55 0.563534346232494 1106 56-57 0.6343635201468558 1106 58-59 0.6820881847427742 1106 60-61 0.5652700631613072 1106 62-63 0.6998160624911947 1106 64-65 0.6588776786908284 1106 66-67 0.5242486344811681 1106 68-69 0.6093495355757454 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1.1729182758440757 2204 52-53 1.0674393338835984 2204 54-55 1.0496504659972956 2204 56-57 1.0710494911767299 2204 58-59 0.9471104634020193 2204 60-61 0.9431829889654253 2204 62-63 0.8360768302583423 2204 64-65 0.9115625745337042 2204 66-67 0.9339910185552966 2204 68-69 0.9410824468816585 2204 70-71 0.9233515410793274 2204 72-73 0.8666437638380415 2204 74-75 0.9227543118109196 2204 76-77 0.8582545414356133 2204 78-79 0.7147314017267377 2204 80-81 0.8262238042280927 2204 82-83 0.8674226116098538 2204 84-85 0.9654064754763994 2204 86-87 0.9914898361645221 2204 88-89 0.9336628229866548 2204 90-91 0.8955820509437693 2204 92-93 1.000860038984353 2204 94-95 1.1297931768014742 2204 96-97 1.0449798182666257 2204 98-99 1.1295114593475901 2204 100-101 1.0716609564254682 2204 102-103 1.179648207474692 2204 104-105 1.1406156725321175 2204 106-107 1.0492545243481963 2204 108-109 1.1712106846911077 2204 110-111 1.1146514459604724 2204 112-113 1.2705648478597524 2204 114-115 1.247485828446223 2204 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-1.3091005235289757 2216 72-73 -1.236939296039509 2216 74-75 -1.1977716496760777 2216 76-77 -1.100388651265277 2216 78-79 -1.07548337600209 2216 80-81 -1.0681561133440312 2216 82-83 -1.3552914764751591 2216 84-85 -1.4112785199328357 2216 86-87 -1.4189677373597434 2216 88-89 -1.8577409212645932 2216 90-91 -1.5088221997324922 2216 92-93 -1.2815822470922527 2216 94-95 -1.317083354310725 2216 96-97 -1.2675898151722471 2216 98-99 -1.318256667991534 2216 100-101 -1.3674078803203997 2216 102-103 -1.6785583480529596 2216 104-105 -1.5693227124250022 2216 106-107 -1.3316144474443448 2216 108-109 -1.2204944442002343 2216 110-111 -1.1870831626306355 2216 112-113 -1.401212251024873 2216 114-115 -1.3171776459587292 2216 116-117 -1.113794169340995 2216 118-119 -0.8979621765643309 2216 120-121 -0.8950375695147059 2216 122-123 -0.9114885646453779 2216 124-125 -0.757781951286681 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28177.0 3 5722.0 4 4023.0 5 3420.0 6 3478.0 7 4051.0 8 4059.0 9 4376.0 10 5184.0 11 5773.0 12 6591.0 13 6663.0 14 6816.0 15 6254.0 16 5672.0 17 5098.0 18 4651.0 19 4717.0 20 4743.0 21 4903.0 22 5746.0 23 7726.0 24 12323.0 25 17468.0 26 19505.0 27 19989.0 28 23362.0 29 27823.0 30 33162.0 31 40739.0 32 50939.0 33 66533.0 34 91698.0 35 135099.0 36 238464.0 37 382830.0 38 148352.0 39 423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.089531284920454 18.807762537804905 12.28224340580758 23.820462771467064 2 15.50568353465183 21.069899560816204 38.98926201499156 24.435154889540403 3 18.325438698366874 26.453594478456356 29.419267333631975 25.801699489544795 4 12.214124638270278 16.890859592871728 36.88288183657075 34.012133932287234 5 12.887979128039854 40.34312363207502 33.536992562912445 13.23190467697268 6 31.491545037942082 37.323847756070144 17.66309701655045 13.52151018943733 7 28.398495180263133 34.24958107278549 20.522179638201738 16.829744108749633 8 26.738820399836577 35.999325287012255 19.70839402408919 17.553460289061977 9 27.28910482057975 16.562257180542918 19.07686436951971 37.071773629357615 10-11 24.548695779023923 28.222423792829048 27.099823771673353 20.129056656473676 12-13 25.02454159073752 25.575483019984457 27.26487773448626 22.135097654791764 14-15 22.14567282963979 26.69659202150312 24.806496839240975 26.35123830961612 16-17 22.43509754811039 30.113403437360226 25.44252850122052 22.008970513308864 18-19 22.617410501003558 28.58716762182504 26.89249611264715 21.90292576452425 20-21 23.456085049716275 27.74556162695749 26.642268895884484 22.15608442744175 22-23 23.32821257728666 27.7057676259903 26.546580097773713 22.419439698949322 24-25 22.864892164769483 27.859718963645168 26.70367357774995 22.571715293835403 26-27 22.641007213394023 28.259241091629494 26.341177255494912 22.75857443948157 28-29 22.63680104422288 28.26277304641171 26.548143037240884 22.552282872124525 30-31 22.66176952552953 27.759136159791353 26.759541335531146 22.819552979147968 32-33 22.519164050912412 28.055852531949878 26.402440057143906 23.022543359993804 34-35 23.080937980350956 27.874293958625916 26.28501171939675 22.759756341626378 36-37 23.119095384981243 27.519221755667672 26.318769365201604 23.04291349414948 38-39 22.59898468735429 27.63904726385098 26.500510352393746 23.261457696400985 40-41 22.848365188921335 27.55128141314928 26.641862445801255 22.95849095212813 42-43 22.800670162656512 27.44476463941426 26.59098819956523 23.163576998364 44-45 22.643471167506025 27.56533671610631 26.724885848694647 23.066306267693022 46-47 22.89420489833616 28.014554811093014 26.207854371697277 22.883385918873554 48-49 22.81025114886068 27.687421401475216 26.26817142584551 23.234156023818596 50-51 22.85071939959239 27.652431689531305 26.473158361931414 23.023690548944888 52-53 23.035386327269862 27.54456864520237 26.438549315789672 22.981495711738095 54-55 22.579353885975976 27.582734618703125 26.49094461405721 23.34696688126369 56-57 22.659847007048157 27.82284924742802 26.485638719129096 23.031665026394723 58-59 22.876489151314676 27.234837472807854 26.427913893583433 23.460759482294037 60-61 22.546681803222928 27.46545187045247 26.805843245380906 23.182023080943704 62-63 22.554181364625062 27.554195192574944 26.715357182859428 23.17626625994057 64-65 22.321478262823405 27.548252504878402 26.604582670061237 23.52568656223696 66-67 22.331812490363653 27.81303518450275 26.392076037078237 23.46307628805536 68-69 22.543172558175872 27.417694370714635 26.753884095587022 23.28524897552247 70-71 22.45850299690976 27.426425346874154 26.68815546599009 23.426916190225995 72-73 22.598234518329793 27.611587118586645 26.32966835425097 23.460510008832593 74-75 22.52691409682645 27.864551104461473 26.334780874207752 23.273753924504327 76-77 22.78520217674828 27.515927574000003 26.470381850429614 23.228488398822098 78-79 22.811561699106285 27.687459685252875 26.517511032327157 22.98346758331369 80-81 22.822624331461604 27.903153153931754 26.38100647459407 22.89321604001257 82-83 22.81099323475694 27.730374866166148 26.318071174547004 23.14056072452991 84-85 22.81768803807638 27.5477130111812 26.531849696755156 23.102749253987263 86-87 22.806002263674525 27.50582339605108 26.437734085310243 23.250440254964147 88-89 22.760697532680208 27.480966434619013 26.456543525960424 23.30179250674035 90-91 22.775631802773415 27.751063519400542 26.459491543352804 23.01381313447324 92-93 22.800208792804252 27.252715343505646 26.510152722948543 23.436923140741563 94-95 22.7342978436271 27.17518641541419 26.706516407425575 23.383999333533133 96-97 22.78121941801808 27.208634839339485 26.56324807567929 23.446897666963146 98-99 22.76192007024057 27.354899815583934 26.33226347818723 23.550916635988266 100-101 22.45721126440191 27.4166288999533 26.671008542363882 23.455151293280913 102-103 22.612269195096584 27.047207905814417 26.683091597393076 23.657431301695926 104-105 22.465869537337046 27.28418205632818 26.681927560074215 23.568020846260556 106-107 22.613800438792826 27.49098926108657 26.31927345808084 23.57593684203977 108-109 22.701082577515475 27.42731341792594 26.197795163019187 23.673808841539397 110-111 22.51511888568757 27.820478031597993 26.41828888262836 23.246114200086073 112-113 22.490275951421932 27.823880299831167 26.505274423505305 23.1805693252416 114-115 22.469521701765196 28.003805422865863 26.20435342035196 23.322319455016984 116-117 22.50101373669395 27.894162086558765 26.35843309666226 23.246391080085026 118-119 22.382126585225258 28.189330491752397 25.945973472419297 23.482569450603048 120-121 22.425882701935905 28.320623328991985 26.138688349679377 23.114805619392733 122-123 22.496111268039513 28.366774065623883 25.986168249761178 23.15094641657542 124-125 22.650219112849353 28.443682038290003 25.812768693887843 23.093330154972794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2427.0 1 1887.5 2 1180.5 3 853.0 4 625.5 5 521.5 6 451.5 7 412.5 8 410.5 9 436.5 10 398.5 11 310.5 12 284.5 13 306.0 14 362.0 15 420.5 16 454.0 17 541.5 18 706.5 19 834.5 20 1052.0 21 1476.5 22 1983.5 23 2622.0 24 3545.5 25 4812.0 26 6508.0 27 8600.5 28 10725.0 29 13430.5 30 16835.0 31 20148.5 32 23918.0 33 26238.5 34 27570.5 35 29259.0 36 31801.5 37 35462.0 38 38157.5 39 39576.5 40 40818.0 41 43641.5 42 46187.0 43 48566.5 44 51138.0 45 53489.0 46 54375.0 47 53997.0 48 53995.5 49 52735.5 50 51551.0 51 50280.5 52 50229.5 53 51340.0 54 51680.5 55 49151.5 56 44079.0 57 40564.0 58 38368.0 59 34678.0 60 28567.0 61 23201.5 62 19242.5 63 14791.0 64 11284.0 65 8936.0 66 6461.0 67 5078.5 68 3734.5 69 2157.0 70 1447.5 71 1078.5 72 900.5 73 672.5 74 541.5 75 480.0 76 326.0 77 175.5 78 88.5 79 54.0 80 35.5 81 21.5 82 12.0 83 10.0 84 9.0 85 8.0 86 6.0 87 5.5 88 3.0 89 1.0 90 2.5 91 2.5 92 1.0 93 2.0 94 2.5 95 3.0 96 3.5 97 2.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09007626410941327 2 3.4564951692023514E-4 3 0.0 4 0.002073897101521411 5 0.0012443382609128467 6 3.4564951692023514E-4 7 0.0 8 7.604289372245173E-4 9 2.7651961353618814E-4 10-11 0.009194277150078256 12-13 0.0019356372947533168 14-15 0.00442431381657901 16-17 0.002419546618441646 18-19 0.01348033115988917 20-21 0.016936826329091523 22-23 0.010438615410991102 24-25 0.010749699976219313 26-27 0.010058400942378844 28-29 0.008122763647625526 30-31 0.018388554300156512 32-33 0.00197020224644534 34-35 0.00127890321260487 36-37 9.678186473766584E-4 38-39 1.7282475846011757E-4 40-41 0.0016591176812171286 42-43 0.0025923713769017637 44-45 4.839093236883292E-4 46-47 0.0013825980676809406 48-49 0.008226458502701596 50-51 0.004700833430115198 52-53 0.007154945000248867 54-55 0.008295588406085644 56-57 0.010058400942378844 58-59 0.009159712198386231 60-61 0.011337304154983713 62-63 0.014240760097113688 64-65 0.008606672971313857 66-67 0.014171630193729641 68-69 0.014171630193729641 70-71 0.004286054009810916 72-73 0.014137065242037616 74-75 0.013203811546352983 76-77 0.00570321702918388 78-79 0.008364718309469691 80-81 0.014275325048805713 82-83 0.018146599638312347 84-85 0.013549461063273217 86-87 0.015969007681714862 88-89 0.015588793213102605 90-91 0.01209773309220823 92-93 0.009816446280534678 94-95 0.008191893551009572 96-97 0.009159712198386231 98-99 0.006532775869792444 100-101 0.011924908333748114 102-103 0.01199403823713216 104-105 0.010611440169451218 106-107 0.005772346932567927 108-109 0.003491060120894375 110-111 0.00687842538671268 112-113 0.00953992666699849 114-115 0.01348033115988917 116-117 0.011786648526980019 118-119 0.2228748085101676 120-121 0.02637305814101394 122-123 0.026718707657934177 124-125 0.2391894657088027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1446552.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.76235838342556 #Duplication Level Percentage of deduplicated Percentage of total 1 84.58428840127345 63.23720883063031 2 9.488049534933483 14.186979193807826 3 2.9065032220794063 6.5189110659504514 4 1.1801621745836934 3.5292682978715573 5 0.6394688850669102 2.3904100980210954 6 0.33321183642761804 1.4947021639560572 7 0.23160031682226126 1.212049012158656 8 0.13477679162877496 0.8060984638014995 9 0.0977259170394046 0.6575598029743991 >10 0.3845007322727898 4.719858428328103 >50 0.016263096918585337 0.8009758901038918 >100 0.0032848485430172604 0.3805308830894221 >500 1.6424241060962848E-4 0.06544786930681298 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.912990338404703E-5 0.0 0.0 0.0 0.0 7 6.912990338404703E-5 0.0 0.0 0.0 0.0 8 6.912990338404703E-5 0.0 0.0 0.0 0.0 9 6.912990338404703E-5 0.0 0.0 0.0 0.0 10-11 6.912990338404703E-5 0.0 0.0 0.0 0.0 12-13 2.073897101521411E-4 0.0 0.0 0.0 0.0 14-15 2.073897101521411E-4 0.0 0.0 0.0 0.0 16-17 2.073897101521411E-4 0.0 0.0 0.0 0.0 18-19 2.419546618441646E-4 0.0 0.0 0.0 0.0 20-21 2.7651961353618814E-4 0.0 0.0 2.073897101521411E-4 0.0 22-23 2.7651961353618814E-4 0.0 0.0 6.567340821484469E-4 0.0 24-25 3.4564951692023514E-4 0.0 0.0 0.00127890321260487 0.0 26-27 3.4564951692023514E-4 0.0 0.0 0.0024886765218256933 0.0 28-29 4.1477942030428215E-4 0.0 0.0 0.006532775869792444 0.0 30-31 4.839093236883292E-4 0.0 0.0 0.017939209928160206 0.0 32-33 5.184742753803527E-4 0.0 0.0 0.04037186357628346 0.0 34-35 5.876041787643998E-4 0.0 0.0 0.07507507507507508 0.0 36-37 6.221691304564232E-4 0.0 0.0 0.1311048617678452 0.0 38-39 6.221691304564232E-4 0.0 0.0 0.22380806220585225 0.0 40-41 6.221691304564232E-4 0.0 0.0 0.32715726776500254 0.0 42-43 6.912990338404703E-4 0.0 0.0 0.4210702415122305 0.0 44-45 7.604289372245173E-4 0.0 0.0 0.5295350599218003 0.0 46-47 7.949938889165409E-4 0.0 0.0 0.6678985615449704 0.0 48-49 8.986887439926114E-4 0.0 0.0 0.8183252313086566 0.0 50-51 8.986887439926114E-4 0.0 0.0 0.986103506821739 3.456495169202352E-5 52-53 8.986887439926114E-4 0.0 0.0 1.143304907117062 6.912990338404703E-5 54-55 8.986887439926114E-4 0.0 0.0 1.3013704312046852 6.912990338404703E-5 56-57 8.986887439926114E-4 0.0 0.0 1.491961574834503 6.912990338404703E-5 58-59 8.986887439926114E-4 0.0 0.0 1.71379943479391 6.912990338404703E-5 60-61 8.986887439926114E-4 0.0 0.0 1.9570329998506795 6.912990338404703E-5 62-63 8.986887439926114E-4 0.0 0.0 2.1969137645933228 6.912990338404703E-5 64-65 8.986887439926114E-4 0.0 0.0 2.4217587753499354 6.912990338404703E-5 66-67 0.0010369485507607055 0.0 0.0 2.6860769609388395 6.912990338404703E-5 68-69 0.0010369485507607055 0.0 0.0 2.9955024084858337 6.912990338404703E-5 70-71 0.0011752083575287996 0.0 0.0 3.341324750164529 6.912990338404703E-5 72-73 0.0012443382609128464 0.0 0.0 3.6798538870362076 6.912990338404703E-5 74-75 0.0012443382609128464 0.0 0.0 4.011228078907637 6.912990338404703E-5 76-77 0.0012443382609128464 0.0 0.0 4.364654709958577 6.912990338404703E-5 78-79 0.0012443382609128464 0.0 0.0 4.7656081495860505 6.912990338404703E-5 80-81 0.0016591176812171288 0.0 0.0 5.210389947959008 6.912990338404703E-5 82-83 0.0017282475846011757 0.0 0.0 5.681959583893285 6.912990338404703E-5 84-85 0.0017282475846011757 0.0 0.0 6.134000022121569 6.912990338404703E-5 86-87 0.0017282475846011757 0.0 0.0 6.580890282547742 6.912990338404703E-5 88-89 0.0017282475846011757 0.0 0.0 7.071090427444019 6.912990338404703E-5 90-91 0.0017973774879852227 0.0 0.0 7.630662430386187 6.912990338404703E-5 92-93 0.0017973774879852227 0.0 0.0 8.217367920406595 6.912990338404703E-5 94-95 0.0017973774879852227 0.0 0.0 8.79207937218987 6.912990338404703E-5 96-97 0.0017973774879852227 0.0 0.0 9.359601314021205 6.912990338404703E-5 98-99 0.0018665073913692698 0.0 0.0 9.951353286988645 6.912990338404703E-5 100-101 0.0019702022464453406 0.0 0.0 10.628065911215081 6.912990338404703E-5 102-103 0.0021084620532134347 0.0 0.0 11.338928707713238 6.912990338404703E-5 104-105 0.0024195466184416463 0.0 0.0 12.037451816457342 6.912990338404703E-5 106-107 0.0025923713769017637 0.0 0.0 12.709014262881666 6.912990338404703E-5 108-109 0.002626936328593787 0.0 0.0 13.407226287060542 6.912990338404703E-5 110-111 0.002696066231977834 0.0 0.0 14.14511887578186 6.912990338404703E-5 112-113 0.002696066231977834 0.0 0.0 14.938177127403646 6.912990338404703E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2955 0.0 52.374 1 GGTATCA 1825 0.0 52.18636 1 TCAACGC 3395 0.0 43.798042 4 ATCAACG 3390 0.0 43.68568 3 CAACGCA 3440 0.0 43.3995 5 AACGCAG 3530 0.0 41.450512 6 TATCAAC 3730 0.0 40.979225 2 GTACATG 8865 0.0 38.407597 1 TACATGG 9055 0.0 36.78231 2 ACATGGG 9190 0.0 34.494602 3 ACGCAGA 4475 0.0 32.697273 7 CGCAGAG 4595 0.0 31.584482 8 CATGGGA 6430 0.0 29.87762 4 CATGGGG 3345 0.0 29.160984 4 GCAGAGT 5330 0.0 26.559458 9 GAGTACA 6955 0.0 26.103731 1 ATGGGGA 1860 0.0 25.90249 5 GTATAGA 535 0.0 25.590214 1 ATGGGAG 2780 0.0 25.246819 5 AGTACAT 6850 0.0 25.179485 2 >>END_MODULE