FastQCFastQC Report
Thu 26 May 2016
SRR1364427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446552
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48410.33465786228217165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43570.30119898904429293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.20172105807464924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.075.490531
GTATCAA59200.055.9511261
ATCAACG76800.043.187133
TATCAAC77400.042.776892
TCAACGC77950.042.5499884
CAACGCA78300.042.3319665
AACGCAG80550.041.1423956
TGGTATC11100.039.6994132
GTACATG88450.037.5830571
TACATGG90500.036.0584752
ACATGGG90200.035.1208843
TATAACG1209.230862E-734.736992
ACGCAGA95200.034.686147
CGCAGAG96800.034.1116338
GTGGTAT14950.032.6759641
GCAGAGT105300.030.8495739
CATGGGA65000.030.2315864
CATGGGG32550.030.1851484
GAGTACT63650.028.08865712-13
CAGAGTA105700.026.87715310-11
Produced by FastQC (version 0.11.5)