FastQCFastQC Report
Thu 26 May 2016
SRR1364426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844508
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21380.11591166858587765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.060.6138151
GTATCAA34300.048.9442021
ATCAACG39050.041.1233523
TCAACGC39950.040.3468864
CAACGCA40950.039.363755
GTACATG108850.038.9948581
TATCAAC44150.037.1812672
AACGCAG44150.036.3759356
TACATGG114550.035.9299622
ACATGGG110700.035.4602743
CATGGGA82500.031.6495934
CGCAGAG54100.029.7956668
ACGCAGA54600.029.6317527
CATGGGG35800.029.2406644
ATGGGAT33650.026.3379695
TATTACG1150.001007358225.8593712
ATGGGAG36050.025.5745245
GAGTACA79350.025.2830351
GCAGAGT63600.024.5026769
AGTACAT81550.023.4113712
Produced by FastQC (version 0.11.5)