##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364426_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1844508 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.63870311215782 31.0 31.0 34.0 27.0 34.0 2 30.624178913835017 31.0 31.0 34.0 26.0 34.0 3 30.596454447473256 31.0 31.0 34.0 26.0 34.0 4 34.1261230637113 37.0 35.0 37.0 30.0 37.0 5 34.3280983329972 37.0 35.0 37.0 30.0 37.0 6 34.418493169994385 37.0 35.0 37.0 31.0 37.0 7 34.31358009832432 37.0 35.0 37.0 30.0 37.0 8 34.150690590661576 37.0 35.0 37.0 30.0 37.0 9 35.22419203386486 39.0 35.0 39.0 28.0 39.0 10-11 35.465919096040786 39.0 35.0 39.0 29.0 39.0 12-13 35.51141876316069 39.0 35.0 39.0 30.0 39.0 14-15 36.66272442298976 40.0 36.5 41.0 29.5 41.0 16-17 36.80988127999444 40.0 37.0 41.0 30.0 41.0 18-19 36.80071081285632 40.0 37.0 41.0 30.0 41.0 20-21 36.76954233866158 40.0 37.0 41.0 29.5 41.0 22-23 36.697845983861285 40.0 36.5 41.0 29.0 41.0 24-25 36.771324927839835 40.0 36.5 41.0 29.5 41.0 26-27 36.798409115059414 40.0 36.0 41.0 30.0 41.0 28-29 36.759088060339124 40.0 36.0 41.0 30.0 41.0 30-31 36.54341401609535 40.0 36.0 41.0 29.5 41.0 32-33 36.622386294881885 40.0 36.0 41.0 30.0 41.0 34-35 36.56857004686344 40.0 36.0 41.0 30.0 41.0 36-37 36.44611950720734 40.0 36.0 41.0 29.0 41.0 38-39 36.30964815549729 40.0 35.5 41.0 29.0 41.0 40-41 36.28093724722257 40.0 35.0 41.0 29.0 41.0 42-43 36.15472201801239 40.0 35.0 41.0 28.0 41.0 44-45 36.13878307928185 40.0 35.0 41.0 28.5 41.0 46-47 36.00141365610776 39.0 35.0 41.0 28.0 41.0 48-49 35.84404811472761 39.0 35.0 41.0 28.0 41.0 50-51 35.718550963183674 39.0 35.0 41.0 27.5 41.0 52-53 35.60497081064435 39.0 35.0 41.0 28.0 41.0 54-55 35.48495289800857 39.0 34.5 40.5 28.0 41.0 56-57 35.447826737536516 39.0 34.5 40.5 28.0 41.0 58-59 35.433241818414444 39.0 34.0 41.0 28.0 41.0 60-61 35.25603738232634 38.0 34.0 40.5 28.0 41.0 62-63 35.00542475283382 38.0 34.0 40.0 28.0 41.0 64-65 34.71360899491897 37.0 34.0 40.0 27.0 41.0 66-67 34.40743520765429 37.0 34.0 40.0 26.5 41.0 68-69 34.11377722406192 36.5 34.0 39.5 26.0 41.0 70-71 33.76369687743289 36.0 33.0 39.0 26.0 41.0 72-73 33.426596414870524 35.5 33.0 39.0 26.0 40.5 74-75 33.07184869894844 35.0 33.0 37.5 26.0 40.0 76-77 32.72030834238724 35.0 33.0 37.0 26.0 39.0 78-79 32.398217573466745 35.0 32.5 37.0 25.0 39.0 80-81 32.06951311677694 35.0 32.0 36.0 25.0 38.5 82-83 31.779787076011598 35.0 32.0 36.0 24.5 37.0 84-85 31.438870690720776 35.0 32.0 35.5 23.0 37.0 86-87 31.240790498062356 35.0 32.0 35.0 23.0 36.5 88-89 31.098102583453148 35.0 32.0 35.0 23.0 36.0 90-91 30.968424371160225 35.0 32.0 35.0 23.0 36.0 92-93 30.74515155260915 35.0 31.0 35.0 20.0 36.0 94-95 30.66817926514821 35.0 31.0 35.0 20.0 35.0 96-97 30.591012887989642 35.0 31.0 35.0 20.0 35.0 98-99 30.438386550776684 34.5 31.0 35.0 19.0 35.0 100-101 30.310592038635775 34.0 31.0 35.0 18.5 35.0 102-103 29.27070985867234 33.5 29.5 34.5 17.0 35.0 104-105 30.226166273065772 34.0 31.0 35.0 18.5 35.0 106-107 30.4760738364919 35.0 32.0 35.0 19.0 35.0 108-109 30.45979415649051 35.0 32.0 35.0 18.0 35.0 110-111 30.359415356290135 35.0 32.0 35.0 17.0 35.0 112-113 30.25544345700859 35.0 32.0 35.0 12.5 35.0 114-115 30.15715654255769 35.0 32.0 35.0 7.5 35.0 116-117 30.03805838738569 35.0 31.0 35.0 2.0 35.0 118-119 29.781619271914245 35.0 31.0 35.0 2.0 35.0 120-121 29.65163772670002 35.0 31.0 35.0 2.0 35.0 122-123 29.418856681564947 35.0 31.0 35.0 2.0 35.0 124-125 28.238668522988245 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.725017323104943 1101 2 0.7423853348416003 1101 3 0.8773608341734018 1101 4 0.878103439140915 1101 5 0.6819365335859118 1101 6 0.7101868912318707 1101 7 0.7264191550807126 1101 8 0.6828318708452343 1101 9 1.21267774590752 1101 10-11 0.9426884191396638 1101 12-13 0.8732458397364482 1101 14-15 1.1308322210785988 1101 16-17 0.8637715136643109 1101 18-19 0.894953320925751 1101 20-21 1.077229613977309 1101 22-23 1.0044911478533578 1101 24-25 1.058523083530126 1101 26-27 1.023795414894252 1101 28-29 0.9624656953581692 1101 30-31 1.0548997591875349 1101 32-33 0.9673721286110037 1101 34-35 0.8621963232140928 1101 36-37 0.9010038488507561 1101 38-39 1.0354573592658198 1101 40-41 0.9886360692925464 1101 42-43 0.9366244286042686 1101 44-45 0.8030522296855978 1101 46-47 0.7988553903545252 1101 48-49 0.7997519525746029 1101 50-51 0.7742629145050017 1101 52-53 0.6957233819617556 1101 54-55 0.7643320429575837 1101 56-57 0.7109114777525818 1101 58-59 0.8272432656880895 1101 60-61 0.7731829063122433 1101 62-63 0.7213583583684269 1101 64-65 0.702952660020074 1101 66-67 0.7245750489263827 1101 68-69 0.636997589209642 1101 70-71 0.665515959595119 1101 72-73 0.6855934380674356 1101 74-75 0.7277526617750496 1101 76-77 0.8278252404613227 1101 78-79 0.6880925149798571 1101 80-81 0.7210550185374345 1101 82-83 0.7995362966313593 1101 84-85 0.8555783786206916 1101 86-87 0.814947118543202 1101 88-89 0.6815047279164297 1101 90-91 0.7949441534597455 1101 92-93 0.983024091221786 1101 94-95 0.8026890889389868 1101 96-97 0.8295792707828653 1101 98-99 0.8512007625334874 1101 100-101 0.7495910844145115 1101 102-103 0.7340865701392865 1101 104-105 0.6441838984726402 1101 106-107 0.5747638729377265 1101 108-109 0.5916241859238021 1101 110-111 0.637395752258179 1101 112-113 0.47960064569937444 1101 114-115 0.517016650414881 1101 116-117 0.5493750359424645 1101 118-119 0.4398554310090752 1101 120-121 0.3657931338943783 1101 122-123 0.45951058856948634 1101 124-125 0.06137956251934895 1102 1 0.46980201755130935 1102 2 0.34787854589108846 1102 3 0.48546627550604526 1102 4 0.444599856479293 1102 5 0.4320476652675467 1102 6 0.22386727753271174 1102 7 0.3408151073444472 1102 8 0.47088264657998025 1102 9 0.8243673747022697 1102 10-11 0.5718006728189593 1102 12-13 0.5173836308149902 1102 14-15 0.6574736894205628 1102 16-17 0.4892735135259656 1102 18-19 0.4655324924581379 1102 20-21 0.670184908015969 1102 22-23 0.6989256884955068 1102 24-25 0.6424922240168414 1102 26-27 0.5889936005741845 1102 28-29 0.5896625169412104 1102 30-31 0.7266247822785061 1102 32-33 0.5449492901094857 1102 34-35 0.4138715379499942 1102 36-37 0.46492071421510417 1102 38-39 0.5975294895762389 1102 40-41 0.5869943466446799 1102 42-43 0.6333929888666248 1102 44-45 0.5350060467155444 1102 46-47 0.5798641812011454 1102 48-49 0.5020090700883486 1102 50-51 0.4936340566843853 1102 52-53 0.4516947389787731 1102 54-55 0.4769190664391729 1102 56-57 0.4692734374542482 1102 58-59 0.6139149404857633 1102 60-61 0.5516956846754866 1102 62-63 0.5523504667474981 1102 64-65 0.6234721459559438 1102 66-67 0.6845962529529572 1102 68-69 0.6102334182672493 1102 70-71 0.6367554872778456 1102 72-73 0.6435134395322422 1102 74-75 0.6081923922921604 1102 76-77 0.6719158832245924 1102 78-79 0.5173124926416293 1102 80-81 0.622190163817308 1102 82-83 0.5865491228041151 1102 84-85 0.6448224848269817 1102 86-87 0.6942186948736264 1102 88-89 0.6132079519050038 1102 90-91 0.5161034476286162 1102 92-93 0.5597218628917027 1102 94-95 0.603887734404001 1102 96-97 0.48915673643122304 1102 98-99 0.5148580578579214 1102 100-101 0.45174291928249843 1102 102-103 0.41268955080796843 1102 104-105 0.4017819232277944 1102 106-107 0.3355689494939753 1102 108-109 0.4754683096263328 1102 110-111 0.4693181116473575 1102 112-113 0.4229714247299192 1102 114-115 0.39086566187695837 1102 116-117 0.404172593181638 1102 118-119 0.4337968470657465 1102 120-121 0.3201346697799927 1102 122-123 0.26256871883107635 1102 124-125 -0.029596341487287958 1103 1 0.5165029428028554 1103 2 0.5891720097846971 1103 3 0.7277394809766271 1103 4 0.5766416141874728 1103 5 0.5031733430210465 1103 6 0.3896184037499708 1103 7 0.4365495088982172 1103 8 0.48942301506207997 1103 9 0.924412693145257 1103 10-11 0.661573945727703 1103 12-13 0.5472024919193572 1103 14-15 0.8114594195060718 1103 16-17 0.6795846731925508 1103 18-19 0.6679191289125086 1103 20-21 0.6667363903067098 1103 22-23 0.7118026003673208 1103 24-25 0.6001206937101387 1103 26-27 0.752190304196958 1103 28-29 0.6441114123041629 1103 30-31 0.8817746298682678 1103 32-33 0.7746427162377287 1103 34-35 0.7705881088544757 1103 36-37 0.7489686222834067 1103 38-39 0.7315051039378702 1103 40-41 0.6921610537742353 1103 42-43 0.7257566963468705 1103 44-45 0.48649858805513446 1103 46-47 0.4974323611801026 1103 48-49 0.488933620275823 1103 50-51 0.4532506033111261 1103 52-53 0.4066498251647417 1103 54-55 0.4014735699594425 1103 56-57 0.5063721129687977 1103 58-59 0.6311223263129548 1103 60-61 0.7148795750480161 1103 62-63 0.6918740835908537 1103 64-65 0.6138987597510948 1103 66-67 0.4891475194415875 1103 68-69 0.5252616074178036 1103 70-71 0.6174898888316207 1103 72-73 0.5093364548272135 1103 74-75 0.5083545136269052 1103 76-77 0.5040780854851192 1103 78-79 0.4365207686525281 1103 80-81 0.47571542615175133 1103 82-83 0.4415027253505812 1103 84-85 0.5572178882420502 1103 86-87 0.5072122747608745 1103 88-89 0.5015861990523689 1103 90-91 0.4836910497974287 1103 92-93 0.47354317874506435 1103 94-95 0.39134572043081306 1103 96-97 0.5648327365391239 1103 98-99 0.4596504832038768 1103 100-101 0.47981351354755475 1103 102-103 0.5128743313641948 1103 104-105 0.5228019928239753 1103 106-107 0.4316847812762319 1103 108-109 0.480842321657299 1103 110-111 0.4984856550100929 1103 112-113 0.5286413554034937 1103 114-115 0.5066483491527265 1103 116-117 0.36992102187252485 1103 118-119 0.3847943923167527 1103 120-121 0.30188536439302993 1103 122-123 0.2953687512013907 1103 124-125 0.08196822380633506 1104 1 0.47999789704507734 1104 2 0.46595486595775526 1104 3 0.5735221935446759 1104 4 0.4113002911936974 1104 5 0.48303861541364057 1104 6 0.3918489811726573 1104 7 0.505311111530105 1104 8 0.6490609998921713 1104 9 1.0407155169419156 1104 10-11 0.7384357193800994 1104 12-13 0.6999670841464081 1104 14-15 0.7310641793593433 1104 16-17 0.6481028653360497 1104 18-19 0.6181133220481883 1104 20-21 0.6589191864267647 1104 22-23 0.7191448633211479 1104 24-25 0.7333351612619339 1104 26-27 0.7007740324440661 1104 28-29 0.6852963494443358 1104 30-31 0.795874161193737 1104 32-33 0.7657185135718976 1104 34-35 0.7974460312816589 1104 36-37 0.6679706980285189 1104 38-39 0.8416000137930837 1104 40-41 0.7552909249213826 1104 42-43 0.7442551754392852 1104 44-45 0.6700444568001629 1104 46-47 0.7450761946505082 1104 48-49 0.6960339740663457 1104 50-51 0.7186169266312774 1104 52-53 0.5936736766510506 1104 54-55 0.540844971674872 1104 56-57 0.6027223916930637 1104 58-59 0.5297142792105163 1104 60-61 0.5598428252209899 1104 62-63 0.5717834818858734 1104 64-65 0.5591526795150514 1104 66-67 0.5605273024674773 1104 68-69 0.5378213014085844 1104 70-71 0.5850900533246275 1104 72-73 0.4648440332551331 1104 74-75 0.48809589406267406 1104 76-77 0.44199026449528844 1104 78-79 0.3947130427031027 1104 80-81 0.41462892371698956 1104 82-83 0.4910632287514076 1104 84-85 0.4393731383105859 1104 86-87 0.49674262111004097 1104 88-89 0.4517542169116098 1104 90-91 0.46640479279668057 1104 92-93 0.4894555274702448 1104 94-95 0.49761022780036157 1104 96-97 0.4686850714260018 1104 98-99 0.41301671513901894 1104 100-101 0.5009724712362988 1104 102-103 0.44764763127673035 1104 104-105 0.502457536630029 1104 106-107 0.46273818928417043 1104 108-109 0.3400842177656038 1104 110-111 0.37778721247167724 1104 112-113 0.4266947242464276 1104 114-115 0.34216291200602456 1104 116-117 0.26931344213911146 1104 118-119 0.20744325196244162 1104 120-121 0.13702524872572752 1104 122-123 0.26928916791701596 1104 124-125 -7.122840583306811E-5 1105 1 0.2338255569937182 1105 2 0.27662181380037865 1105 3 0.2767267619941478 1105 4 0.15599366602332765 1105 5 0.21186064803664806 1105 6 0.161287870192659 1105 7 0.22940048082046616 1105 8 0.38106288387257337 1105 9 0.7978084214869909 1105 10-11 0.3228382445837994 1105 12-13 0.3045127071021412 1105 14-15 0.12468996106274943 1105 16-17 0.2286787456667625 1105 18-19 0.17762435249462527 1105 20-21 0.45911619992193664 1105 22-23 0.5386636514832333 1105 24-25 0.4112882003096132 1105 26-27 0.3914539631042473 1105 28-29 0.30986015240495846 1105 30-31 0.5019562153414299 1105 32-33 0.3385644025028256 1105 34-35 0.32602047426524194 1105 36-37 0.2868543914218762 1105 38-39 0.4392556838914743 1105 40-41 0.3947900434550675 1105 42-43 0.3783672874995787 1105 44-45 0.28114061669284496 1105 46-47 0.36374145645248035 1105 48-49 0.2288200293630993 1105 50-51 0.2778010392762482 1105 52-53 0.06538992595667992 1105 54-55 0.0379458521768683 1105 56-57 0.1815912954342025 1105 58-59 0.24607353021664125 1105 60-61 0.18495476903249397 1105 62-63 0.19709926557629842 1105 64-65 0.17241367855758938 1105 66-67 -0.003302684542092038 1105 68-69 0.002238649254451275 1105 70-71 0.18192833375602646 1105 72-73 0.14571154482001702 1105 74-75 0.18462361209902411 1105 76-77 0.13121711436280492 1105 78-79 0.008629399962025275 1105 80-81 0.03150416305280146 1105 82-83 0.16758183902148716 1105 84-85 0.2224278714433936 1105 86-87 0.11768063729186906 1105 88-89 0.10606424080902954 1105 90-91 0.06927300324115038 1105 92-93 -0.0013884267264963057 1105 94-95 0.1211869331337958 1105 96-97 0.0937956195084837 1105 98-99 0.14334378773951428 1105 100-101 0.02954913469786291 1105 102-103 -0.027758418015849884 1105 104-105 -0.06330191886308967 1105 106-107 -0.06480829927732401 1105 108-109 0.021393477564824792 1105 110-111 -0.017402673923594847 1105 112-113 0.050480408279263145 1105 114-115 -0.07253171644202894 1105 116-117 -0.113205128035478 1105 118-119 -0.37401130322760423 1105 120-121 -0.2738589760597314 1105 122-123 -0.17028039686676166 1105 124-125 -0.30812936838628957 1106 1 0.26057336790776375 1106 2 0.29796524355330334 1106 3 0.4106474487614449 1106 4 0.1943380720809742 1106 5 0.25011993775089536 1106 6 0.07202463827443495 1106 7 0.021487346102063043 1106 8 0.29510576325714055 1106 9 0.713761352149092 1106 10-11 0.1828658111328707 1106 12-13 0.3950872626859052 1106 14-15 0.561513241801272 1106 16-17 0.38589673824846216 1106 18-19 0.3383592206580204 1106 20-21 0.46467941425958514 1106 22-23 0.4856520021970212 1106 24-25 0.3719593832089245 1106 26-27 0.47717258063244117 1106 28-29 0.4218676872279232 1106 30-31 0.46874728866358595 1106 32-33 0.36830901800747995 1106 34-35 0.4150500162227857 1106 36-37 0.41178745096430447 1106 38-39 0.5413880762838943 1106 40-41 0.5161531563623996 1106 42-43 0.3758686415080774 1106 44-45 0.26153155909795345 1106 46-47 0.41390120158982313 1106 48-49 0.31384669661747466 1106 50-51 0.3538548986691197 1106 52-53 0.2699690839027227 1106 54-55 0.1995078181414769 1106 56-57 0.1919887887578895 1106 58-59 0.2437213046015856 1106 60-61 0.16502475189270172 1106 62-63 0.1624266417788789 1106 64-65 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-1.6514320599751429 2216 86-87 -1.719008073416866 2216 88-89 -2.160147045510435 2216 90-91 -1.918921762748809 2216 92-93 -1.649230900260708 2216 94-95 -1.6735990445738729 2216 96-97 -1.6721781625090628 2216 98-99 -1.6772003823087722 2216 100-101 -1.6981657240122416 2216 102-103 -1.9241491435088136 2216 104-105 -1.7659189718157684 2216 106-107 -1.5268122843646417 2216 108-109 -1.4859927624281184 2216 110-111 -1.5679807847985607 2216 112-113 -1.7706376306398077 2216 114-115 -1.6001985710031157 2216 116-117 -1.464866915199032 2216 118-119 -1.3249153773730455 2216 120-121 -1.182980986645088 2216 122-123 -1.0613363826941544 2216 124-125 -1.0374328217132174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30367.0 3 6100.0 4 3987.0 5 3345.0 6 3679.0 7 4310.0 8 4681.0 9 5011.0 10 5507.0 11 6340.0 12 7055.0 13 7394.0 14 7526.0 15 7029.0 16 6488.0 17 5924.0 18 5696.0 19 5431.0 20 5351.0 21 5663.0 22 6633.0 23 9240.0 24 14057.0 25 20032.0 26 23446.0 27 24178.0 28 28290.0 29 34359.0 30 41400.0 31 50050.0 32 64080.0 33 83736.0 34 115113.0 35 168606.0 36 302086.0 37 510493.0 38 211275.0 39 550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.658776976894735 19.05935825772034 12.392753090767608 23.889111674617315 2 15.38752215368818 20.8922630022971 39.51431850673976 24.20589633727496 3 18.330037061373545 26.283323249343454 29.53725329464551 25.849386394637484 4 12.05401024145213 16.661362508911797 37.524485419891406 33.76014182974467 5 12.948046575753914 39.91864369288021 33.9872126492088 13.146097082157077 6 31.41942920094421 37.25601815557803 17.922344350941337 13.402208292536413 7 28.274694390048726 33.579089925335104 21.155505966902826 16.990709717713344 8 26.386349786715417 36.07355769960596 20.042244877158055 17.497847636520568 9 26.940811569971036 16.325679764532268 19.566614023645446 37.16689464185126 10-11 24.43572281185266 27.82210435939151 27.687583855045343 20.054588973710487 12-13 24.675271867304684 25.296725447907413 27.82106040447479 22.20694228031311 14-15 22.224830691941474 26.36556552497019 25.19397707353915 26.215626709549184 16-17 22.409290327337708 29.769888791058175 25.856566922713252 21.964253958890858 18-19 22.437049494197222 28.52671809327007 27.220602018222618 21.815630394310087 20-21 23.393667121248683 27.731030238289545 26.974950994612794 21.90035164584898 22-23 23.003478552720367 27.731203385129817 26.801854252138273 22.46346381001154 24-25 22.72815012300053 27.882867276003214 26.91849107060073 22.470491530395524 26-27 22.557656402030133 28.199198670288254 26.658676323035802 22.584468604645807 28-29 22.549077017002233 27.99935586923495 26.960776340231174 22.490790773531646 30-31 22.49417085100153 27.85167391469379 27.01731935060569 22.63683588369899 32-33 22.428617512395192 27.951411384873122 26.83360757726495 22.786363525466736 34-35 22.919538971082954 27.870194520632612 26.624018762981276 22.586247745303154 36-37 22.892640125606672 27.523572937313766 26.73481746696102 22.848969470118536 38-39 22.56152734744552 27.686669323205955 26.931778444623355 22.82002488472517 40-41 22.68174597580877 27.517037414541846 27.02822491013679 22.772991699512595 42-43 22.704432795126152 27.43888851840948 26.891816941246123 22.96486174521824 44-45 22.54571113269491 27.65202639970225 26.92632464086966 22.875937826733182 46-47 22.633371926057027 27.786842384189974 26.620657200380272 22.959128489372723 48-49 22.602320822665593 27.547352828590732 26.833170131851613 23.017156216892058 50-51 22.70833474525155 27.494534182201157 26.9480201743911 22.849110898156194 52-53 22.8011195217417 27.62621051541484 26.73094012608174 22.841729836761715 54-55 22.435697413690974 27.469187595714793 26.93368626773765 23.161428722856577 56-57 22.47605130923124 27.7326313535026 26.957760568668725 22.833556768597425 58-59 22.590324373415747 27.315142396269636 26.992693803966223 23.10183942634839 60-61 22.48256708767004 27.50280664398578 27.140796335923227 22.873829932420946 62-63 22.394835149849353 27.56996056089366 27.09534655416838 22.939857735088605 64-65 22.291645035106157 27.497425223027598 26.94597970162137 23.26495004024487 66-67 22.34026517098605 27.72485636390846 26.765981074087808 23.168897391017673 68-69 22.345477516361843 27.53214078504308 27.0421259821172 23.08025571647788 70-71 22.38777566600223 27.496483310873693 26.980548622479866 23.135192400644215 72-73 22.375621256600258 27.502621667608334 26.9233876608652 23.19836941492621 74-75 22.32378460196238 27.67715074191748 26.825310025829047 23.1737546302911 76-77 22.507488290777466 27.53134590793272 26.862801283575315 23.098364517714494 78-79 22.60921828692634 27.48900144984585 26.891550012091088 23.010230251136722 80-81 22.525355302280424 27.894316329305386 26.743001654611565 22.83732671380263 82-83 22.5795116809089 27.66225111337772 26.73735998390598 23.0208772218074 84-85 22.61440222703056 27.378481056928578 26.87290954938581 23.134207166655056 86-87 22.597819420894496 27.298286672352994 26.979365997736675 23.124527909015832 88-89 22.568145710087244 27.289435584884586 26.97095233189279 23.17146637313538 90-91 22.634043187702485 27.616488864187826 26.87717395724607 22.872293990863618 92-93 22.679853116396718 27.130307010587927 26.99155649718663 23.19828337582873 94-95 22.57171640059251 27.102152088434504 27.048501692739112 23.27762981823387 96-97 22.695516906148523 27.171345242632956 26.982523183228157 23.150614667990364 98-99 22.74262406363995 27.168158770229063 26.729934677094043 23.359282489036943 100-101 22.3271254424122 27.28894684547055 27.04744502295596 23.336482689161294 102-103 22.60087167091224 27.014180105559156 27.030500890865454 23.35444733266315 104-105 22.399847963667437 27.203252623223168 27.008245964855966 23.38865344825343 106-107 22.520392865953333 27.269700904632682 26.894943870483274 23.314962358930707 108-109 22.625043780368394 27.29837446067466 26.672274529747337 23.40430722920961 110-111 22.40578972294898 27.57226513205176 26.753205631294996 23.26873951370426 112-113 22.438130548433115 27.583069596660465 26.809670953144426 23.169128901761997 114-115 22.371124349935123 27.64834167372016 26.736664824895257 23.24386915144946 116-117 22.373486159462704 27.775095301702645 26.66585153132031 23.185567007514337 118-119 22.38946631189971 27.8711211400212 26.398769436069415 23.34064311200968 120-121 22.38607748187302 27.89294825855997 26.605858822004596 23.115115437562412 122-123 22.49422722968847 27.93665625091513 26.51428794545776 23.05482857393864 124-125 22.53326978633863 28.0015086071035 26.421818719336038 23.043402887221838 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1605.0 1 1292.5 2 840.0 3 630.5 4 485.5 5 383.0 6 333.0 7 295.0 8 296.0 9 292.5 10 284.0 11 276.0 12 264.5 13 296.0 14 339.5 15 383.0 16 451.5 17 534.0 18 675.0 19 870.5 20 1098.0 21 1471.5 22 2121.0 23 3054.5 24 4267.0 25 5704.5 26 7761.5 27 10717.0 28 13585.5 29 17052.5 30 21205.0 31 24556.0 32 28471.0 33 32320.0 34 36025.5 35 39877.5 36 44986.0 37 50148.5 38 53409.0 39 55434.0 40 57160.0 41 60698.5 42 63767.0 43 66191.0 44 68518.0 45 70554.5 46 70840.0 47 70189.0 48 70696.0 49 70322.0 50 68946.5 51 66672.0 52 65495.5 53 64875.5 54 63776.0 55 60098.0 56 52926.5 57 46864.5 58 43206.0 59 38692.0 60 32062.0 61 25616.0 62 20214.5 63 15726.5 64 12076.5 65 9221.5 66 6840.5 67 5615.5 68 4137.5 69 2369.5 70 1644.0 71 1330.5 72 1108.0 73 825.0 74 633.5 75 520.5 76 337.5 77 167.5 78 86.0 79 54.0 80 34.5 81 22.0 82 14.0 83 11.5 84 11.0 85 11.5 86 8.0 87 6.0 88 5.0 89 3.5 90 3.0 91 2.0 92 2.5 93 2.5 94 2.0 95 1.0 96 0.0 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09113541388814796 2 3.7950499537003907E-4 3 0.0 4 0.0023312449715588114 5 0.0015722349808187334 6 3.2528999603146204E-4 7 0.0 8 0.0013011599841258482 9 4.8793499404719306E-4 10-11 0.009731592381274573 12-13 0.0022770299722202343 14-15 0.0048251349411333536 16-17 0.0028191799656060047 18-19 0.014177222327037888 20-21 0.017511444786360374 22-23 0.011195397363416152 24-25 0.011222504863085441 26-27 0.010761677368707536 28-29 0.008782829892849476 30-31 0.017782519783053258 32-33 0.0023312449715588114 34-35 0.001355374983464425 36-37 0.00108429998677154 38-39 1.6264499801573102E-4 40-41 0.0020601699748659264 42-43 0.0027649649662674276 44-45 9.487624884250976E-4 46-47 0.0017619874785037527 48-49 0.008782829892849476 50-51 0.004906457440141219 52-53 0.008078034901447975 54-55 0.008755722393180187 56-57 0.01087010736738469 58-59 0.009921344878959593 60-61 0.012117052352171961 62-63 0.014773587319762235 64-65 0.00940630238524311 66-67 0.01431275982538433 68-69 0.01507176981612441 70-71 0.004798027441464066 72-73 0.014204329826707175 74-75 0.014448297323730772 76-77 0.006966627415007145 78-79 0.009433409884912399 80-81 0.014556727322407927 82-83 0.01870417477180907 84-85 0.014014577329022157 86-87 0.017294584789006067 88-89 0.01615606980289595 90-91 0.012632094845888443 92-93 0.01032795737399892 94-95 0.00867439989417232 96-97 0.009704484881605285 98-99 0.007156379912692165 100-101 0.011683332357463345 102-103 0.012686309845227018 104-105 0.011493579859778327 106-107 0.006126294925259202 108-109 0.004011909951054699 110-111 0.007400347409715761 112-113 0.009812914880282438 114-115 0.014583834822077214 116-117 0.012659202345557731 118-119 0.21962496232057546 120-121 0.027243037167634945 122-123 0.027432789665319965 124-125 0.23854599708973884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1844508.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.29714356480129 #Duplication Level Percentage of deduplicated Percentage of total 1 84.42270269167199 59.34674851245002 2 9.30345869606866 13.08013143213475 3 2.8753597395237254 6.063887292292469 4 1.2614007158354967 3.5469146885532417 5 0.637780773849688 2.2417083311090793 6 0.3742261262212242 1.578421663240371 7 0.2396949591483205 1.1794909668506068 8 0.15344743512357856 0.8629533101226187 9 0.1119617248994685 0.7083530504118651 >10 0.5598463392964359 7.035451384238393 >50 0.041637531537304734 2.0092530223117073 >100 0.018322542783556175 2.105125678766019 >500 0.0 0.0 >1k 1.6072404051455528E-4 0.2415606675188386 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2138 0.11591166858587765 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.4214999338577005E-5 0.0 5 0.0 0.0 0.0 5.4214999338577005E-5 0.0 6 0.0 0.0 0.0 1.0842999867715401E-4 0.0 7 0.0 0.0 0.0 1.0842999867715401E-4 0.0 8 0.0 0.0 0.0 1.0842999867715401E-4 0.0 9 0.0 0.0 0.0 1.0842999867715401E-4 0.0 10-11 0.0 0.0 0.0 2.9818249636217356E-4 0.0 12-13 0.0 0.0 0.0 3.7950499537003907E-4 0.0 14-15 0.0 0.0 0.0 3.7950499537003907E-4 0.0 16-17 0.0 0.0 0.0 5.96364992724347E-4 0.0 18-19 0.0 0.0 0.0 8.132249900786551E-4 0.0 20-21 0.0 0.0 0.0 9.758699880943861E-4 0.0 22-23 0.0 0.0 0.0 0.0010300849874329632 0.0 24-25 0.0 0.0 0.0 0.0014095899828030021 0.0 26-27 0.0 0.0 0.0 0.001978847475858061 0.0 28-29 0.0 0.0 0.0 0.004282984947747584 0.0 30-31 0.0 0.0 0.0 0.01398746982935287 0.0 32-33 0.0 0.0 0.0 0.033396439592563436 0.0 34-35 0.0 0.0 0.0 0.06324179672845008 0.0 36-37 0.0 0.0 0.0 0.11458340110208251 0.0 38-39 0.0 0.0 0.0 0.19661069510134954 0.0 40-41 0.0 0.0 0.0 0.29064661145411136 0.0 42-43 0.0 0.0 0.0 0.3788002003786376 0.0 44-45 0.0 0.0 0.0 0.4851158140815871 0.0 46-47 0.0 0.0 0.0 0.6115994075384872 0.0 48-49 0.0 0.0 0.0 0.7492783983588036 0.0 50-51 0.0 0.0 0.0 0.9003213865160791 0.0 52-53 0.0 0.0 0.0 1.0439369197639696 0.0 54-55 0.0 0.0 0.0 1.1942208979305051 0.0 56-57 0.0 0.0 0.0 1.3803952056591786 0.0 58-59 0.0 0.0 0.0 1.5892313831113771 0.0 60-61 0.0 0.0 0.0 1.8158771878462985 0.0 62-63 0.0 0.0 0.0 2.039107447622889 0.0 64-65 0.0 0.0 0.0 2.26767788483433 0.0 66-67 0.0 0.0 0.0 2.52254259672498 0.0 68-69 0.0 0.0 0.0 2.8098278782201 0.0 70-71 0.0 0.0 0.0 3.1272024843481296 0.0 72-73 0.0 0.0 0.0 3.4527364478766156 0.0 74-75 0.0 0.0 0.0 3.773851888959007 0.0 76-77 0.0 0.0 0.0 4.12042127223086 0.0 78-79 0.0 0.0 0.0 4.498218495121734 0.0 80-81 0.0 2.7107499669288502E-5 0.0 4.923047229938824 0.0 82-83 0.0 5.4214999338577005E-5 0.0 5.366959644523092 0.0 84-85 0.0 5.4214999338577005E-5 0.0 5.8095166841238965 0.0 86-87 0.0 5.4214999338577005E-5 0.0 6.25288694871478 0.0 88-89 0.0 5.4214999338577005E-5 0.0 6.736240775317863 0.0 90-91 0.0 5.4214999338577005E-5 0.0 7.277279361217191 0.0 92-93 0.0 5.4214999338577005E-5 0.0 7.848000659254392 0.0 94-95 0.0 5.4214999338577005E-5 0.0 8.413951037349797 0.0 96-97 0.0 5.4214999338577005E-5 0.0 8.97353115302292 0.0 98-99 0.0 5.4214999338577005E-5 0.0 9.553848505943048 0.0 100-101 0.0 5.4214999338577005E-5 0.0 10.195970958109154 0.0 102-103 0.0 5.4214999338577005E-5 0.0 10.885612857195522 0.0 104-105 0.0 5.4214999338577005E-5 0.0 11.57758600125345 0.0 106-107 0.0 5.4214999338577005E-5 0.0 12.243048010634816 0.0 108-109 0.0 5.4214999338577005E-5 0.0 12.936322314676868 0.0 110-111 0.0 5.4214999338577005E-5 0.0 13.681968308079988 0.0 112-113 0.0 5.4214999338577005E-5 0.0 14.483699718298864 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1925 0.0 60.613815 1 GTATCAA 3430 0.0 48.944202 1 ATCAACG 3905 0.0 41.123352 3 TCAACGC 3995 0.0 40.346886 4 CAACGCA 4095 0.0 39.36375 5 GTACATG 10885 0.0 38.994858 1 TATCAAC 4415 0.0 37.181267 2 AACGCAG 4415 0.0 36.375935 6 TACATGG 11455 0.0 35.929962 2 ACATGGG 11070 0.0 35.460274 3 CATGGGA 8250 0.0 31.649593 4 CGCAGAG 5410 0.0 29.795666 8 ACGCAGA 5460 0.0 29.631752 7 CATGGGG 3580 0.0 29.240664 4 ATGGGAT 3365 0.0 26.337969 5 TATTACG 115 0.0010073582 25.859371 2 ATGGGAG 3605 0.0 25.574524 5 GAGTACA 7935 0.0 25.283035 1 GCAGAGT 6360 0.0 24.502676 9 AGTACAT 8155 0.0 23.411371 2 >>END_MODULE