FastQCFastQC Report
Thu 26 May 2016
SRR1364426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844508
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45440.24635295699449392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42030.2278656422200392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25710.1393867632994815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.077.813621
GTATCAA64600.052.209821
ATCAACG83050.040.591293
TCAACGC84100.040.0845034
CAACGCA85700.039.0873345
TATCAAC87750.038.42032
AACGCAG89600.037.318576
GTACATG108500.034.4903951
TGGTATC14300.033.32312
TACATGG111650.033.183572
ACATGGG111500.032.157163
ACGCAGA108500.030.763087
CGCAGAG110050.030.2749168
CATGGGA82850.029.4745674
GTGGTAT18850.028.7672481
GCAGAGT121350.027.1615739
ATGGGAT34050.026.560425
CGTAGGG900.00947980926.473352
GAGTACA82300.025.7761731
GAGTACT67900.025.41049612-13
Produced by FastQC (version 0.11.5)