FastQCFastQC Report
Thu 26 May 2016
SRR1364425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003795
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32000.053.7585141
GGTATCA21200.051.1016541
TCAACGC39000.043.0067834
GTACATG114700.041.932171
ATCAACG40200.041.5750473
CAACGCA42050.039.7508965
TACATGG120350.039.635172
AACGCAG42400.039.4217836
ACATGGG119200.038.321053
TATCAAC45800.037.2708132
CATGGGA81550.033.2577864
ACGCAGA52750.031.4613637
CATGGGG45800.031.4269584
CGCAGAG53450.030.9380478
GTACCGT1352.3367666E-630.8432466
ATGGGAT38100.028.1030985
GCAGAGT62550.025.4860949
GAGTACA80600.025.405211
AGTACAT76250.025.117042
ATGGGAG30950.024.9855775
Produced by FastQC (version 0.11.5)