##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364425_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2003795 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.667097682148125 31.0 31.0 34.0 27.0 34.0 2 30.72524634506025 31.0 31.0 34.0 27.0 34.0 3 30.64171684229175 31.0 31.0 34.0 26.0 34.0 4 34.227478858865304 37.0 35.0 37.0 30.0 37.0 5 34.4084429794465 37.0 35.0 37.0 30.0 37.0 6 34.4664923308023 37.0 35.0 37.0 31.0 37.0 7 34.37556436661435 37.0 35.0 37.0 30.0 37.0 8 34.255309051075585 37.0 35.0 37.0 30.0 37.0 9 35.33846126974067 39.0 35.0 39.0 28.0 39.0 10-11 35.55173258741538 39.0 35.0 39.0 29.5 39.0 12-13 35.62094126395165 39.0 35.0 39.0 30.0 39.0 14-15 36.75153645956797 40.0 36.0 41.0 30.0 41.0 16-17 36.884866964934034 40.0 36.5 41.0 30.0 41.0 18-19 36.857777866498324 40.0 36.5 41.0 30.0 41.0 20-21 36.80839057887658 40.0 37.0 41.0 30.0 41.0 22-23 36.70193233339738 40.0 36.0 41.0 29.5 41.0 24-25 36.76182967818564 40.0 36.0 41.0 30.0 41.0 26-27 36.76619215039462 40.0 36.0 41.0 30.0 41.0 28-29 36.7126437584683 40.0 36.0 41.0 30.0 41.0 30-31 36.50299531638716 40.0 36.0 41.0 29.5 41.0 32-33 36.57888257032281 40.0 36.0 41.0 30.0 41.0 34-35 36.52931986555511 40.0 36.0 41.0 30.0 41.0 36-37 36.41584867713514 40.0 36.0 41.0 29.5 41.0 38-39 36.275279656851126 40.0 35.5 41.0 29.0 41.0 40-41 36.25160258409667 40.0 35.0 41.0 29.0 41.0 42-43 36.12286860681856 39.5 35.0 41.0 28.5 41.0 44-45 36.10073685182367 39.0 35.0 41.0 28.5 41.0 46-47 35.96164028755436 39.0 35.0 41.0 28.0 41.0 48-49 35.798742885375006 39.0 35.0 41.0 28.0 41.0 50-51 35.660955836300616 39.0 35.0 41.0 27.5 41.0 52-53 35.530111613213926 39.0 34.5 40.0 28.0 41.0 54-55 35.398332414243974 38.5 34.0 40.0 28.0 41.0 56-57 35.35592213774363 38.5 34.0 40.0 27.5 41.0 58-59 35.341774233392144 38.5 34.0 40.0 28.0 41.0 60-61 35.15659760604254 38.0 34.0 40.0 28.0 41.0 62-63 34.90144301188495 38.0 34.0 40.0 27.0 41.0 64-65 34.61078728113405 37.0 34.0 40.0 26.5 41.0 66-67 34.304581806023066 37.0 34.0 40.0 26.0 41.0 68-69 34.004649427710916 36.0 33.0 39.0 26.0 41.0 70-71 33.666745350697056 36.0 33.0 39.0 26.0 41.0 72-73 33.3254077887209 35.0 33.0 38.5 26.0 40.0 74-75 32.96408914085522 35.0 33.0 37.5 26.0 39.5 76-77 32.61527202133951 35.0 32.5 37.0 25.5 39.0 78-79 32.30376859908324 35.0 32.0 36.5 25.0 39.0 80-81 31.982533392887 35.0 32.0 36.0 25.0 38.0 82-83 31.696447490886044 35.0 32.0 36.0 24.0 37.0 84-85 31.35707070833094 35.0 32.0 35.0 23.0 37.0 86-87 31.164984192494742 35.0 32.0 35.0 23.0 36.0 88-89 31.034255001135346 35.0 32.0 35.0 23.0 36.0 90-91 30.902288657272827 35.0 32.0 35.0 22.5 36.0 92-93 30.676962962778127 34.0 31.0 35.0 20.0 35.5 94-95 30.594130387589548 34.0 31.0 35.0 20.0 35.0 96-97 30.522370801404335 34.0 31.0 35.0 20.0 35.0 98-99 30.37750568296657 34.0 31.0 35.0 18.5 35.0 100-101 30.260135143565087 34.0 31.0 35.0 18.5 35.0 102-103 29.209971828455505 33.5 29.0 34.5 17.0 35.0 104-105 30.18808760377184 34.0 31.0 35.0 18.5 35.0 106-107 30.44289810085363 35.0 31.5 35.0 19.0 35.0 108-109 30.43626368964889 35.0 32.0 35.0 18.0 35.0 110-111 30.342360620722182 35.0 32.0 35.0 17.5 35.0 112-113 30.236421889464744 35.0 31.5 35.0 14.0 35.0 114-115 30.142013030275052 35.0 31.0 35.0 9.0 35.0 116-117 30.01970161618329 35.0 31.0 35.0 4.5 35.0 118-119 29.75755903173728 34.5 31.0 35.0 2.0 35.0 120-121 29.621050806095433 34.0 31.0 35.0 2.0 35.0 122-123 29.379385615794032 34.0 31.0 35.0 2.0 35.0 124-125 28.15443545871708 33.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5938503060488323 1101 2 0.5082003274180629 1101 3 0.7198564045294518 1101 4 0.5701033401127873 1101 5 0.44529561497343195 1101 6 0.4316379862701112 1101 7 0.5213473306252752 1101 8 0.6520098166912192 1101 9 1.108367278984261 1101 10-11 0.8151326366366618 1101 12-13 0.656070706901204 1101 14-15 0.9229428663064283 1101 16-17 0.8024656368031273 1101 18-19 0.8968287848421568 1101 20-21 0.8818550640210319 1101 22-23 0.9208872463976547 1101 24-25 0.9427005439958691 1101 26-27 0.8411651865779035 1101 28-29 0.76649559249757 1101 30-31 1.0189842536693874 1101 32-33 0.8678679446003841 1101 34-35 0.7722808600581459 1101 36-37 0.8337249746587716 1101 38-39 0.9501181845503908 1101 40-41 0.8712269975552616 1101 42-43 0.7793141468105986 1101 44-45 0.6323018602728325 1101 46-47 0.6058628403846171 1101 48-49 0.5377287773685282 1101 50-51 0.6214271872491466 1101 52-53 0.420741897145156 1101 54-55 0.5897988306918549 1101 56-57 0.5395141662471943 1101 58-59 0.6095881976158921 1101 60-61 0.5942954940930463 1101 62-63 0.574619105408587 1101 64-65 0.5020105998555593 1101 66-67 0.6268520939946853 1101 68-69 0.5920069313264733 1101 70-71 0.45809738049153736 1101 72-73 0.5995979596378689 1101 74-75 0.5339437712462569 1101 76-77 0.6430626129768555 1101 78-79 0.43893172206273334 1101 80-81 0.42364459697331114 1101 82-83 0.47174710019521626 1101 84-85 0.5386840225378009 1101 86-87 0.4073737883451116 1101 88-89 0.40061935617401545 1101 90-91 0.32641376903662334 1101 92-93 0.4483209707153897 1101 94-95 0.33670275665426885 1101 96-97 0.3643616318724767 1101 98-99 0.3167237659307105 1101 100-101 0.2937649246378271 1101 102-103 0.2667000550107801 1101 104-105 0.2545678943305738 1101 106-107 0.1719176191317402 1101 108-109 0.2842278736640793 1101 110-111 0.3287399153825987 1101 112-113 0.2043467964017509 1101 114-115 0.28127131463762467 1101 116-117 0.2503366868987875 1101 118-119 0.17591224153222385 1101 120-121 0.14544926763493038 1101 122-123 0.19911836187359455 1101 124-125 0.007147115264690029 1102 1 0.2777073582503924 1102 2 0.18499738477023797 1102 3 0.21747610857638122 1102 4 0.10791650961727584 1102 5 0.1453319836540672 1102 6 0.021773775266851203 1102 7 0.1328891963049088 1102 8 0.2107989264200114 1102 9 0.5779619697897402 1102 10-11 0.2705754909054292 1102 12-13 0.21559260012222836 1102 14-15 0.3765660106284443 1102 16-17 0.1843127962744333 1102 18-19 0.13939859602000126 1102 20-21 0.3510409516664126 1102 22-23 0.32006709845479975 1102 24-25 0.30046715587305073 1102 26-27 0.16068139840469087 1102 28-29 0.22408872076653097 1102 30-31 0.39494102403664044 1102 32-33 0.1715641886435222 1102 34-35 0.09567248501344494 1102 36-37 0.11144226272495672 1102 38-39 0.09443947994910218 1102 40-41 0.155099809858676 1102 42-43 0.19516852346948355 1102 44-45 -0.055312149110342546 1102 46-47 0.05476356817673178 1102 48-49 -0.018432866156942396 1102 50-51 -0.03907592542201144 1102 52-53 -0.13580854319351943 1102 54-55 -0.19979023383891814 1102 56-57 -0.059674698873386944 1102 58-59 0.05591714013449689 1102 60-61 0.01833445706608927 1102 62-63 0.0875233522762926 1102 64-65 0.12918247509638547 1102 66-67 0.09249252012578779 1102 68-69 -0.07057658624047747 1102 70-71 0.044047811092433165 1102 72-73 0.025112952098233166 1102 74-75 0.021788180253153655 1102 76-77 0.08598909853231618 1102 78-79 -0.09000807118391663 1102 80-81 -0.02505239271453874 1102 82-83 -0.14769717044125485 1102 84-85 0.02263336932547233 1102 86-87 -0.006943774236731315 1102 88-89 0.025844389299319204 1102 90-91 -0.163701513454523 1102 92-93 -0.08790021414825944 1102 94-95 -0.0495830048184267 1102 96-97 -0.08851443870950959 1102 98-99 -0.12341397776777896 1102 100-101 -0.19830332436075793 1102 102-103 -0.23721679983633592 1102 104-105 -0.23180216939678644 1102 106-107 -0.2763583186121856 1102 108-109 -0.22604768212193704 1102 110-111 -0.1774649612461232 1102 112-113 -0.16719224593320803 1102 114-115 -0.17425564794672965 1102 116-117 -0.27728319781769883 1102 118-119 -0.1640016658492094 1102 120-121 -0.19047781372179884 1102 122-123 -0.1497976871056217 1102 124-125 -0.4324015640050902 1103 1 0.37008641225767747 1103 2 0.4578668031719175 1103 3 0.5641754323575832 1103 4 0.4189521806099634 1103 5 0.40533945200152033 1103 6 0.33614237216050213 1103 7 0.3707169164333237 1103 8 0.5252786412323047 1103 9 0.9962808698569106 1103 10-11 0.6640499025385935 1103 12-13 0.6024897994307636 1103 14-15 0.6651637626415905 1103 16-17 0.5805368157072266 1103 18-19 0.6135930395328018 1103 20-21 0.5907288775016255 1103 22-23 0.5528635725303275 1103 24-25 0.5426928518611476 1103 26-27 0.7283446161002942 1103 28-29 0.5489536283923684 1103 30-31 0.7550129815541169 1103 32-33 0.6345966333427313 1103 34-35 0.564068000375805 1103 36-37 0.5548126465782346 1103 38-39 0.664857810368737 1103 40-41 0.7089870051904228 1103 42-43 0.6908269221134731 1103 44-45 0.5263447903209055 1103 46-47 0.5238922579160317 1103 48-49 0.5432260591248124 1103 50-51 0.4989945516785781 1103 52-53 0.4298978267889879 1103 54-55 0.3124733615148827 1103 56-57 0.3182653257907191 1103 58-59 0.4379973776699444 1103 60-61 0.44527417070196407 1103 62-63 0.4595725471352239 1103 64-65 0.4731791358500246 1103 66-67 0.36841885363510585 1103 68-69 0.4232466846857008 1103 70-71 0.4667007177587763 1103 72-73 0.3920035740415386 1103 74-75 0.4359224202815142 1103 76-77 0.3944107969462536 1103 78-79 0.2870338862285564 1103 80-81 0.26884181018209574 1103 82-83 0.21125453495884017 1103 84-85 0.34310233995252304 1103 86-87 0.3151563870473595 1103 88-89 0.36946885599702384 1103 90-91 0.33233035353345386 1103 92-93 0.2627962964126844 1103 94-95 0.22808849167182643 1103 96-97 0.32098547051020887 1103 98-99 0.3261936893278303 1103 100-101 0.2683579359264421 1103 102-103 0.20299758026992976 1103 104-105 0.11932810881903677 1103 106-107 0.1599654051959689 1103 108-109 0.17714715173077877 1103 110-111 0.15142669664239605 1103 112-113 0.10383730439907879 1103 114-115 0.05997343173491032 1103 116-117 0.02078985436419245 1103 118-119 0.021871393244424553 1103 120-121 0.031474978696984124 1103 122-123 0.09143809094765132 1103 124-125 -0.1878441247572873 1104 1 0.43419122427267354 1104 2 0.40676310676833083 1104 3 0.5052423371436596 1104 4 0.3860851134962786 1104 5 0.4177192777609875 1104 6 0.3325315939907938 1104 7 0.41773146232986136 1104 8 0.47106453432499507 1104 9 0.9554184486569994 1104 10-11 0.7067685314144398 1104 12-13 0.6018888891368945 1104 14-15 0.662885526112369 1104 16-17 0.49620365048352255 1104 18-19 0.539551968103865 1104 20-21 0.6048123509205325 1104 22-23 0.5769344888056835 1104 24-25 0.6065175622264576 1104 26-27 0.5775418804234747 1104 28-29 0.574105599434418 1104 30-31 0.62459585004936 1104 32-33 0.6557119403610372 1104 34-35 0.608716484793419 1104 36-37 0.40886378999132234 1104 38-39 0.6151471688202435 1104 40-41 0.5799838814367746 1104 42-43 0.5540714874430321 1104 44-45 0.4449694234628083 1104 46-47 0.4335793863539479 1104 48-49 0.4680857946012793 1104 50-51 0.4245941537194966 1104 52-53 0.2174504201216223 1104 54-55 0.2987384775699482 1104 56-57 0.41940469423916227 1104 58-59 0.3320808531737143 1104 60-61 0.45707278814440144 1104 62-63 0.45939502085705897 1104 64-65 0.36869819746049615 1104 66-67 0.41641068012928173 1104 68-69 0.41281031821392133 1104 70-71 0.4388323236283398 1104 72-73 0.3286094925987726 1104 74-75 0.3151302778051601 1104 76-77 0.24742461564179052 1104 78-79 0.19851832489839438 1104 80-81 0.35785205792037544 1104 82-83 0.3437131144192094 1104 84-85 0.3324610312242129 1104 86-87 0.29827054196911007 1104 88-89 0.3424877284059811 1104 90-91 0.28721131502328134 1104 92-93 0.3416186936199672 1104 94-95 0.350046542931576 1104 96-97 0.19479217180926867 1104 98-99 0.29199058842431214 1104 100-101 0.32802990739381244 1104 102-103 0.32552933433171205 1104 104-105 0.30419514549369 1104 106-107 0.2723964560952439 1104 108-109 0.1521494607190732 1104 110-111 0.19342823610691795 1104 112-113 0.2835546717229427 1104 114-115 0.20723267826877034 1104 116-117 0.15498278112516672 1104 118-119 -0.032274434325128 1104 120-121 -0.11960952652174939 1104 122-123 -0.1306743341613803 1104 124-125 -0.4217636110131231 1105 1 0.28766536991040326 1105 2 0.313855893273022 1105 3 0.4317040266781156 1105 4 0.22915133863000392 1105 5 0.2370317873105705 1105 6 0.23846756168138228 1105 7 0.32432834990304116 1105 8 0.4214427656891786 1105 9 0.9302321129352009 1105 10-11 0.42793114634123697 1105 12-13 0.5814728503127569 1105 14-15 0.36402725371346634 1105 16-17 0.3046039275598673 1105 18-19 0.40769824250767783 1105 20-21 0.6547099208799025 1105 22-23 0.7355213994567151 1105 24-25 0.6721106207701126 1105 26-27 0.6442710125888453 1105 28-29 0.6699564877989488 1105 30-31 0.7115626750901569 1105 32-33 0.6325761911377015 1105 34-35 0.5101088373107814 1105 36-37 0.5109215145081265 1105 38-39 0.5940497261955926 1105 40-41 0.5075155421536977 1105 42-43 0.5417252762049358 1105 44-45 0.5138574142642653 1105 46-47 0.519458787078122 1105 48-49 0.4229173446841443 1105 50-51 0.49617029296499027 1105 52-53 0.3866344662430521 1105 54-55 0.38189534613071174 1105 56-57 0.40690234538173087 1105 58-59 0.4629855776639715 1105 60-61 0.43561217401564534 1105 62-63 0.46145229598178616 1105 64-65 0.5077454360924989 1105 66-67 0.4727150134603164 1105 68-69 0.4929020715348784 1105 70-71 0.5320863005914589 1105 72-73 0.4272501129186992 1105 74-75 0.528029094984312 1105 76-77 0.4888666228483629 1105 78-79 0.43653591222311405 1105 80-81 0.4139342557026424 1105 82-83 0.3837525898553338 1105 84-85 0.5171243351840253 1105 86-87 0.45476464416839235 1105 88-89 0.39925122547897374 1105 90-91 0.29859007917834646 1105 92-93 0.26822857803005107 1105 94-95 0.3428462367597085 1105 96-97 0.3651833059515539 1105 98-99 0.3037141303612678 1105 100-101 0.016903858350712397 1105 102-103 0.09374852521277433 1105 104-105 0.11977952972517159 1105 106-107 0.11359038842432057 1105 108-109 0.21628675020762245 1105 110-111 0.22622523567278563 1105 112-113 0.2712366258719925 1105 114-115 0.1188269102483055 1105 116-117 0.08881173121359254 1105 118-119 -0.07649231548955981 1105 120-121 -0.12235599142600506 1105 122-123 -0.02370266404693311 1105 124-125 -0.2692391039910085 1106 1 0.3927745465457271 1106 2 0.35809481757216943 1106 3 0.425342487296934 1106 4 0.40266713572024315 1106 5 0.37984595339314353 1106 6 0.22960604378858562 1106 7 0.20454280010116577 1106 8 0.5078898640145724 1106 9 0.9732439526458947 1106 10-11 0.41926859137980443 1106 12-13 0.5154396222334512 1106 14-15 0.8475475653185285 1106 16-17 0.6022815798715087 1106 18-19 0.5421172863760262 1106 20-21 0.6289489096442082 1106 22-23 0.6743906980992023 1106 24-25 0.6165579628910294 1106 26-27 0.6101046721549679 1106 28-29 0.5667591427477987 1106 30-31 0.7294957598614502 1106 32-33 0.6759117729377451 1106 34-35 0.6390473656766176 1106 36-37 0.6040666408246835 1106 38-39 0.8254399037365587 1106 40-41 0.7433149414165285 1106 42-43 0.7747118455721775 1106 44-45 0.4861115597648791 1106 46-47 0.5886181359721689 1106 48-49 0.5419732172259728 1106 50-51 0.5645129366304857 1106 52-53 0.47612690858188955 1106 54-55 0.2987054966197533 1106 56-57 0.386816417227962 1106 58-59 0.5125927894792994 1106 60-61 0.4489558764725814 1106 62-63 0.5426626254886173 1106 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-0.43033401420796125 2216 98-99 -0.40257314722462567 2216 100-101 -0.5162041248765412 2216 102-103 -0.818639120608605 2216 104-105 -0.7210558475782243 2216 106-107 -0.3517065616001247 2216 108-109 -0.3497854645061338 2216 110-111 -0.2949149338259218 2216 112-113 -0.49137357844800533 2216 114-115 -0.3045585459260245 2216 116-117 -0.34773727722027203 2216 118-119 -0.1306230281773182 2216 120-121 0.0641586834447807 2216 122-123 0.06278021216870044 2216 124-125 0.071880247175244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31649.0 3 6111.0 4 4152.0 5 3659.0 6 4004.0 7 4596.0 8 4991.0 9 5538.0 10 6316.0 11 6533.0 12 7232.0 13 7357.0 14 7542.0 15 7411.0 16 7128.0 17 6889.0 18 6316.0 19 6190.0 20 6165.0 21 6498.0 22 7814.0 23 9968.0 24 14656.0 25 20395.0 26 24276.0 27 27023.0 28 32361.0 29 38658.0 30 47600.0 31 57987.0 32 72982.0 33 95514.0 34 131032.0 35 192309.0 36 342154.0 37 547666.0 38 194904.0 39 219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.976116228526955 18.55646415286413 12.14318574496238 24.324233873646534 2 15.782258402585503 20.20872467546467 39.59036584708562 24.418651074864208 3 18.660641432881107 25.52591457708997 29.037102098767587 26.77634189126133 4 12.495770413556237 15.923706744680702 37.23493343953463 34.345589402228434 5 13.32936413669474 38.98617801297957 34.23417128963291 13.450286560692776 6 32.276835396922834 36.47647707594109 17.78639478188832 13.460292745247756 7 29.32495589618698 32.486257326722544 21.03738156847382 17.151405208616648 8 26.761324619119105 35.29037177808298 20.00742597975023 17.940877623047687 9 27.40461417229311 15.82965820151184 19.547276326827806 37.218451299367246 10-11 24.856890100031567 26.876487153860456 27.789635496795174 20.476987249312806 12-13 25.3856570824135 24.225061390973103 27.82597477586462 22.563306750748783 14-15 22.72382683461571 25.599327844495917 25.00315665545243 26.673688665435947 16-17 22.942973696214384 28.938630821732538 25.603071652937352 22.515323829115726 18-19 22.957984896154514 27.933204876693857 26.849816785827212 22.258993441324424 20-21 23.861991203746108 27.11645041232284 26.63633257656167 22.385225807369377 22-23 23.543536319320744 27.122114510708954 26.5246907695786 22.809658400391694 24-25 23.234977041325614 27.297788979836294 26.565507087242963 22.90172689159513 26-27 23.08533676581638 27.48818004365136 26.464249654247872 22.962233536284383 28-29 23.061568303432285 27.239446875718226 26.707763608818265 22.991221212031224 30-31 23.189739015545065 27.03770801476565 26.73550182723809 23.037051142451197 32-33 22.97437676154306 27.29056430513326 26.67619048807297 23.05886844525071 34-35 23.371076363823285 27.218924132740817 26.366505728328534 23.043493775107365 36-37 23.395221334067944 26.976451019347923 26.359043228775995 23.269284417808134 38-39 23.109344347134353 27.0486064151465 26.77811885383142 23.063930383887723 40-41 23.140211691702063 26.889183002181394 26.84374349661113 23.126861809505417 42-43 23.16001550096282 26.795979431529886 26.640146345857623 23.40385872164967 44-45 22.961640643695187 27.01875884736531 26.66295533753923 23.356645171400274 46-47 23.27076840846839 27.105112716926715 26.429532301422554 23.194586573182345 48-49 23.144859438763223 27.022224928423604 26.50641074430714 23.32650488850603 50-51 23.09625074362281 26.939907291695782 26.619226193090395 23.344615771591013 52-53 23.20376114531267 26.871776187897556 26.61110126793918 23.313361398850596 54-55 23.106026009779246 26.817113530385583 26.614830774728482 23.46202968510669 56-57 23.038117906826667 27.111038685884754 26.589826607020665 23.261016800267917 58-59 23.16047531753633 26.674349393026354 26.650542590469595 23.514632698967723 60-61 23.09922738298446 26.914398227173358 26.71832419968257 23.268050190159613 62-63 22.928174934235205 26.820407762953753 26.84266834502804 23.408748957783008 64-65 23.066703133921333 26.753098385725067 26.66178883862441 23.51840964172919 66-67 22.92706969849353 27.029070050373775 26.443676075512005 23.60018417562069 68-69 22.764092920878497 26.771015693531304 26.876654109678782 23.58823727591142 70-71 22.904265656402824 26.61739451145655 26.860968777277648 23.617371054862975 72-73 23.004570173361127 26.60206250503797 26.59584848676177 23.797518834839128 74-75 22.95524625887553 26.796643144990224 26.50912733548885 23.738983260645398 76-77 22.862181448316544 26.7316904213152 26.793177346859125 23.612950783509127 78-79 22.904044802616045 26.52551639946038 26.8086271288205 23.761811669103075 80-81 22.888389050256407 26.940276326523787 26.62036684650709 23.550967776712717 82-83 23.159485661990693 26.649458177156266 26.626547909288316 23.56450825156472 84-85 23.04608158191645 26.435674376086983 26.684059176125114 23.83418486587146 86-87 22.9074265406865 26.46846253288012 26.916630479812724 23.707480446620647 88-89 23.028582551990397 26.383544755539695 26.828592035418108 23.7592806570518 90-91 23.08775185425775 26.53578160323763 26.75553993894382 23.620926603560804 92-93 23.163967710347404 26.229977430782576 26.721489268891112 23.88456558997891 94-95 22.996542807647447 26.11652539940878 26.98424225001485 23.902689542928925 96-97 23.117436337025403 26.208470429111347 26.77848940728457 23.895603826578675 98-99 23.06908362791733 26.22052101315933 26.66053792210605 24.049857436817284 100-101 22.864791307361994 26.16660523497808 26.925796093543536 24.042807364116392 102-103 23.03603447350788 25.90282084332073 26.914166854872622 24.14697782829877 104-105 22.802878927287395 26.22582874220744 26.842046552856157 24.129245777649007 106-107 22.80495336722917 26.08785052559344 26.957996194516358 24.149199912661032 108-109 23.01175232896839 26.10695400404396 26.64400227475701 24.23729139223064 110-111 22.79955142336242 26.388014798836736 26.7633259152825 24.049107862518348 112-113 22.726451481527324 26.278992697174296 26.980601994002818 24.01395382729556 114-115 22.675423513516346 26.452393941314785 26.703449948765606 24.16873259640326 116-117 22.640803146984993 26.376612529094963 26.906991200949705 24.07559312297034 118-119 22.637026552567484 26.33728112512066 26.647369500307587 24.37832282200427 120-121 22.588299482452918 26.477290425378975 26.796214837947062 24.138195254221042 122-123 22.680334920813596 26.52832517878808 26.70046790774827 24.09087199265005 124-125 22.783109971471777 26.59400131961164 26.45606238491541 24.166826324001175 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 452.0 1 346.0 2 226.0 3 179.0 4 140.5 5 121.5 6 101.0 7 97.0 8 91.5 9 98.5 10 104.5 11 93.5 12 100.5 13 119.0 14 127.5 15 146.5 16 183.0 17 225.5 18 289.0 19 376.5 20 510.0 21 673.0 22 957.5 23 1397.5 24 1973.0 25 2913.0 26 4423.0 27 6438.0 28 8804.0 29 12067.0 30 16730.5 31 21397.0 32 26216.0 33 31310.0 34 36396.0 35 41633.0 36 48769.5 37 55541.5 38 59486.0 39 62828.5 40 63322.0 41 64595.0 42 67012.5 43 70113.0 44 75334.0 45 78891.5 46 79169.5 47 78185.5 48 78009.5 49 76849.5 50 75508.5 51 74124.0 52 73639.0 53 73614.0 54 72978.5 55 69249.0 56 61950.0 57 55829.0 58 51604.5 59 45609.5 60 37448.0 61 30466.5 62 24649.0 63 19432.5 64 15223.0 65 11877.0 66 9167.5 67 7502.0 68 5471.0 69 3398.0 70 2403.0 71 1732.5 72 1372.0 73 1165.5 74 927.0 75 740.5 76 534.0 77 303.5 78 172.5 79 105.0 80 63.5 81 47.0 82 33.5 83 27.0 84 19.0 85 12.5 86 7.5 87 4.5 88 3.5 89 4.5 90 5.5 91 5.0 92 4.0 93 3.5 94 5.5 95 4.0 96 2.5 97 2.0 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09022879086932545 2 3.4933713279052995E-4 3 0.0 4 0.0026449811482711556 5 0.0015470644452152042 6 2.495265234218071E-4 7 0.0 8 9.482007890028671E-4 9 5.988636562123371E-4 10-11 0.008858191581474153 12-13 0.0019712595350322762 14-15 0.004591288030961251 16-17 0.0026449811482711556 18-19 0.013249858393697958 20-21 0.016393892588812728 22-23 0.009581818499397394 24-25 0.009731534413450477 26-27 0.009581818499397394 28-29 0.007909990792471285 30-31 0.018040767643396655 32-33 0.002370501972507168 34-35 0.0012725852694512162 36-37 6.986742655810599E-4 38-39 2.9943182810616856E-4 40-41 0.0015969697498995656 42-43 0.002420407277191529 44-45 0.0010230587460294091 46-47 0.0018215436209791922 48-49 0.008309233229946178 50-51 0.004541382726276889 52-53 0.007485795702654214 54-55 0.008184469968235273 56-57 0.00978143971813484 58-59 0.008284280577603996 60-61 0.010729640507137705 62-63 0.01265099473748562 64-65 0.008533807101025803 66-67 0.013599195526488488 68-69 0.013100142479644875 70-71 0.004691098640329973 72-73 0.01300033187027615 74-75 0.01262604208514344 76-77 0.005838920648070287 78-79 0.00808465935886655 80-81 0.01295042656559179 82-83 0.016294081979444004 84-85 0.012501278823432537 86-87 0.015221117928730234 88-89 0.014971591405308429 90-91 0.011503172729745308 92-93 0.009032860147869418 94-95 0.007286174483916769 96-97 0.008858191581474153 98-99 0.006088447171492093 100-101 0.010704687854795525 102-103 0.011353456815692223 104-105 0.010055918893898827 106-107 0.005364820253568853 108-109 0.003218892152141312 110-111 0.006188257780860817 112-113 0.009332291975975586 114-115 0.012875568608565247 116-117 0.011303551511007861 118-119 0.2178366549472376 120-121 0.02490274703749635 122-123 0.025726184564788316 124-125 0.23565284871955466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2003795.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.20990316146663 #Duplication Level Percentage of deduplicated Percentage of total 1 85.44290744101879 55.71723720062991 2 8.56918446781615 11.175913786380702 3 2.561653748778596 5.011355786731807 4 1.108059201364781 2.8902573287267765 5 0.5654293420825883 1.8435796320928683 6 0.36456983212538496 1.42641380690931 7 0.2268040397371879 1.0352908627524016 8 0.16682185888944706 0.8702749810717355 9 0.12281854165166882 0.7208086686784091 >10 0.7324900490963634 9.31106210857707 >50 0.09139207660568727 4.22150283648683 >100 0.04678145991697204 5.2954173839662735 >500 0.0010879409164803648 0.4808856169958931 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.9905304684361424E-5 0.0 0.0 0.0 0.0 7 4.9905304684361424E-5 0.0 0.0 0.0 0.0 8 4.9905304684361424E-5 0.0 0.0 0.0 0.0 9 4.9905304684361424E-5 0.0 0.0 0.0 0.0 10-11 4.9905304684361424E-5 0.0 0.0 0.0 0.0 12-13 9.981060936872285E-5 0.0 0.0 0.0 0.0 14-15 1.4971591405308428E-4 0.0 0.0 4.9905304684361424E-5 0.0 16-17 1.4971591405308428E-4 0.0 0.0 4.9905304684361424E-5 0.0 18-19 1.996212187374457E-4 0.0 0.0 4.9905304684361424E-5 0.0 20-21 2.495265234218071E-4 2.4952652342180712E-5 0.0 9.981060936872285E-5 0.0 22-23 2.7447917576398784E-4 4.9905304684361424E-5 0.0 1.74668566395265E-4 0.0 24-25 4.990530468436142E-4 4.9905304684361424E-5 0.0 3.992424374748914E-4 0.0 26-27 4.990530468436142E-4 4.9905304684361424E-5 0.0 9.731534413450478E-4 0.0 28-29 5.739110038701564E-4 4.9905304684361424E-5 0.0 0.003967471722406734 0.0 30-31 5.988636562123371E-4 4.9905304684361424E-5 0.0 0.013973485311621198 0.0 32-33 6.238163085545178E-4 4.9905304684361424E-5 0.0 0.0358320087633715 0.0 34-35 6.737216132388792E-4 4.9905304684361424E-5 0.0 0.06757178254262536 0.0 36-37 6.986742655810599E-4 4.9905304684361424E-5 0.0 0.12107026916426081 0.0 38-39 7.485795702654213E-4 4.9905304684361424E-5 0.0 0.20199172070995286 0.0 40-41 7.485795702654213E-4 4.9905304684361424E-5 0.0 0.2863815909312081 0.0 42-43 7.485795702654213E-4 4.9905304684361424E-5 0.0 0.37316691577731254 0.0 44-45 7.984848749497828E-4 4.9905304684361424E-5 0.0 0.4760217487317815 0.0 46-47 9.731534413450478E-4 4.9905304684361424E-5 0.0 0.604378192379959 0.0 48-49 0.00104801139837159 4.9905304684361424E-5 0.0 0.743838566320407 0.0 50-51 0.00104801139837159 4.9905304684361424E-5 0.0 0.8915582681861168 0.0 52-53 0.00104801139837159 4.9905304684361424E-5 0.0 1.037132041950399 0.0 54-55 0.00104801139837159 4.9905304684361424E-5 0.0 1.1941590831397422 0.0 56-57 0.0010979167030559513 4.9905304684361424E-5 0.0 1.3802809169600683 0.0 58-59 0.0010979167030559513 4.9905304684361424E-5 0.0 1.5859406775643217 0.0 60-61 0.0010979167030559513 4.9905304684361424E-5 0.0 1.8107391225150278 0.0 62-63 0.0011478220077403127 4.9905304684361424E-5 0.0 2.0372093951726598 0.0 64-65 0.0011478220077403127 4.9905304684361424E-5 0.0 2.2661250277598257 0.0 66-67 0.0011977273124246742 4.9905304684361424E-5 0.0 2.5237861158451835 0.0 68-69 0.0011977273124246742 4.9905304684361424E-5 0.0 2.8118145818309754 0.0 70-71 0.0012725852694512162 4.9905304684361424E-5 0.0 3.1373718369394075 0.0 72-73 0.0013723958788199391 4.9905304684361424E-5 0.0 3.4623052757392845 0.0 74-75 0.0014223011835043007 4.9905304684361424E-5 0.0 3.784968023176024 0.0 76-77 0.0014472538358464813 4.9905304684361424E-5 0.0 4.1389463493021985 0.0 78-79 0.001472206488188662 4.9905304684361424E-5 0.0 4.539910519788701 0.0 80-81 0.0015969697498995656 4.9905304684361424E-5 0.0 4.977929379003341 0.0 82-83 0.0015969697498995656 4.9905304684361424E-5 0.0 5.42817503786565 0.0 84-85 0.0017217330116104691 4.9905304684361424E-5 0.0 5.87580066823203 0.0 86-87 0.0018464962733213727 4.9905304684361424E-5 0.0 6.333906412582126 0.0 88-89 0.0018464962733213727 4.9905304684361424E-5 0.0 6.83285964881637 0.0 90-91 0.001896401578005734 4.9905304684361424E-5 0.0 7.385560898195674 0.0 92-93 0.001996212187374457 4.9905304684361424E-5 0.0 7.962042025257075 0.0 94-95 0.001996212187374457 4.9905304684361424E-5 0.0 8.542665292607278 0.0 96-97 0.0020461174920588182 4.9905304684361424E-5 0.0 9.131348266664006 0.0 98-99 0.0022207860584540834 4.9905304684361424E-5 0.0 9.74301263352788 0.0 100-101 0.002345549320164987 4.9905304684361424E-5 0.0 10.427039692184081 0.0 102-103 0.00244535992953371 4.9905304684361424E-5 0.0 11.148271155482472 0.0 104-105 0.0025451705389024325 4.9905304684361424E-5 0.0 11.864661804226479 0.0 106-107 0.002694886452955517 4.9905304684361424E-5 0.0 12.562387869018536 0.0 108-109 0.0027447917576398782 4.9905304684361424E-5 0.0 13.284717249019984 0.0 110-111 0.0028446023670086014 4.9905304684361424E-5 0.0 14.050089954311694 0.0 112-113 0.0028945076716929627 4.9905304684361424E-5 0.0 14.853789933600993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3200 0.0 53.758514 1 GGTATCA 2120 0.0 51.101654 1 TCAACGC 3900 0.0 43.006783 4 GTACATG 11470 0.0 41.93217 1 ATCAACG 4020 0.0 41.575047 3 CAACGCA 4205 0.0 39.750896 5 TACATGG 12035 0.0 39.63517 2 AACGCAG 4240 0.0 39.421783 6 ACATGGG 11920 0.0 38.32105 3 TATCAAC 4580 0.0 37.270813 2 CATGGGA 8155 0.0 33.257786 4 ACGCAGA 5275 0.0 31.461363 7 CATGGGG 4580 0.0 31.426958 4 CGCAGAG 5345 0.0 30.938047 8 GTACCGT 135 2.3367666E-6 30.843246 6 ATGGGAT 3810 0.0 28.103098 5 GCAGAGT 6255 0.0 25.486094 9 GAGTACA 8060 0.0 25.40521 1 AGTACAT 7625 0.0 25.11704 2 ATGGGAG 3095 0.0 24.985577 5 >>END_MODULE