FastQCFastQC Report
Thu 26 May 2016
SRR1364425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003795
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20400.1018068215560973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.063.680371
GTATCAA47800.055.2194181
ATCAACG62950.041.435963
TCAACGC63000.041.308544
CAACGCA66550.039.1622165
TATCAAC69450.037.9065362
AACGCAG70000.037.315226
GTACATG106150.037.0458831
TACATGG114150.034.0722922
ACATGGG115350.033.144883
ACGCAGA82750.031.4219347
CGCAGAG84350.030.8941358
CATGGGA81200.029.702844
GCAGAGT99700.025.8989169
ATGGGAG31900.025.1816565
AGTACAT76200.025.0126132
CATGGGG41750.024.9620694
ATTCCGT3101.2732926E-1124.951766
GTGGTAT15250.024.2235531
GAGTACA82350.023.8039111
Produced by FastQC (version 0.11.5)