FastQCFastQC Report
Thu 26 May 2016
SRR1364424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309810
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.045.8108631
GTATCAA32700.045.32561
ATCAACG32050.043.425143
TCAACGC32600.043.05744
CAACGCA32950.042.4222835
AACGCAG35550.039.152356
TATCAAC37450.037.9576642
GTACATG94200.037.660611
TACATGG98150.034.7230382
ACATGGG95200.034.4245343
ACGCAGA43700.031.9865977
CATGGGA62300.030.741234
CGCAGAG45800.030.3900898
GAGTACA69250.024.75511
ATGGGAT28000.024.6423195
ATGGGAG30250.023.595955
CATGGGG43000.023.099444
GCAGAGT58500.023.0813399
TAAGGTG13050.022.789835
AGTACAT74150.021.6573852
Produced by FastQC (version 0.11.5)