##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364424_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2309810 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.83305466683407 31.0 31.0 34.0 27.0 34.0 2 30.821806555517554 31.0 31.0 34.0 27.0 34.0 3 30.780046843679784 31.0 31.0 34.0 27.0 34.0 4 34.37631796554695 37.0 35.0 37.0 30.0 37.0 5 34.53125018940952 37.0 35.0 37.0 31.0 37.0 6 34.635611587100236 37.0 35.0 37.0 31.0 37.0 7 34.507950870417915 37.0 35.0 37.0 31.0 37.0 8 34.334833168096075 37.0 35.0 37.0 30.0 37.0 9 35.44215974474091 39.0 35.0 39.0 29.0 39.0 10-11 35.672034929279896 39.0 35.0 39.0 30.0 39.0 12-13 35.746946069157204 39.0 35.0 39.0 30.0 39.0 14-15 36.916871084634664 40.0 37.0 41.0 30.0 41.0 16-17 37.062813824513704 40.0 37.0 41.0 31.0 41.0 18-19 37.044576177261334 40.0 37.0 41.0 30.0 41.0 20-21 36.997781419250934 40.0 37.0 41.0 30.0 41.0 22-23 36.91208952251484 40.0 37.0 41.0 30.0 41.0 24-25 36.964495781038266 40.0 37.0 41.0 30.0 41.0 26-27 36.98148397487239 40.0 37.0 41.0 30.0 41.0 28-29 36.9345708088544 40.0 36.5 41.0 30.0 41.0 30-31 36.70786168559319 40.0 36.0 41.0 30.0 41.0 32-33 36.78889021174902 40.0 36.0 41.0 30.0 41.0 34-35 36.74045852256246 40.0 36.0 41.0 30.0 41.0 36-37 36.62672146193843 40.0 36.0 41.0 30.0 41.0 38-39 36.48500439430083 40.0 36.0 41.0 29.5 41.0 40-41 36.45932327767218 40.0 36.0 41.0 29.5 41.0 42-43 36.32016724319316 40.0 35.0 41.0 29.0 41.0 44-45 36.307602356903814 40.0 35.0 41.0 29.0 41.0 46-47 36.164271736636344 39.5 35.0 41.0 29.0 41.0 48-49 35.99993982189011 39.0 35.0 41.0 28.0 41.0 50-51 35.86825171767374 39.0 35.0 41.0 28.0 41.0 52-53 35.74095618254315 39.0 35.0 41.0 28.0 41.0 54-55 35.61774583190825 39.0 35.0 40.5 28.0 41.0 56-57 35.579059533035185 39.0 34.5 40.5 28.0 41.0 58-59 35.56047986630935 39.0 35.0 41.0 28.0 41.0 60-61 35.374695537728215 38.0 34.0 40.5 28.0 41.0 62-63 35.12200808724527 38.0 34.0 40.0 28.0 41.0 64-65 34.81013979504807 37.0 34.0 40.0 28.0 41.0 66-67 34.50219000697027 37.0 34.0 40.0 27.0 41.0 68-69 34.198640147890956 36.0 34.0 39.0 26.5 41.0 70-71 33.847647209077806 36.0 33.0 39.0 26.0 41.0 72-73 33.518347829475154 35.0 33.0 39.0 26.0 40.5 74-75 33.16296773327676 35.0 33.0 37.5 26.0 40.0 76-77 32.81244517947364 35.0 33.0 37.0 26.0 39.0 78-79 32.494962139743095 35.0 33.0 36.5 26.0 39.0 80-81 32.178694567951474 35.0 32.0 36.0 25.0 38.5 82-83 31.888045553530375 35.0 32.0 36.0 25.0 37.0 84-85 31.54983505136786 35.0 32.0 35.0 24.0 37.0 86-87 31.359368303020595 35.0 32.0 35.0 24.0 36.5 88-89 31.218434200215604 35.0 32.0 35.0 24.0 36.0 90-91 31.096184534658697 35.0 32.0 35.0 23.0 36.0 92-93 30.876335282988645 35.0 32.0 35.0 21.5 36.0 94-95 30.796571146544522 35.0 32.0 35.0 21.5 35.0 96-97 30.721697888570922 35.0 32.0 35.0 20.0 35.0 98-99 30.573967555772988 34.5 31.0 35.0 19.5 35.0 100-101 30.44582866123188 34.0 31.0 35.0 19.0 35.0 102-103 29.40917651235383 33.5 29.5 34.5 18.0 35.0 104-105 30.368622310926007 34.0 31.0 35.0 19.0 35.0 106-107 30.614735411137712 35.0 32.0 35.0 19.5 35.0 108-109 30.607569670232614 35.0 32.0 35.0 19.0 35.0 110-111 30.50323641338465 35.0 32.0 35.0 18.5 35.0 112-113 30.39794420320286 35.0 32.0 35.0 17.5 35.0 114-115 30.296845411527357 35.0 32.0 35.0 14.0 35.0 116-117 30.177945372130175 35.0 32.0 35.0 6.5 35.0 118-119 29.91669747728168 35.0 31.0 35.0 2.0 35.0 120-121 29.789970387174705 35.0 31.0 35.0 2.0 35.0 122-123 29.560962157060537 35.0 31.0 35.0 2.0 35.0 124-125 28.393001588875276 34.0 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6773185242410094 1101 2 0.623955598872918 1101 3 0.7694194957749012 1101 4 0.6917868910240585 1101 5 0.5289905134522428 1101 6 0.5313224996237409 1101 7 0.5840105178091264 1101 8 0.6659526748767419 1101 9 1.0975494194388205 1101 10-11 0.9483586779704112 1101 12-13 0.8325542654677847 1101 14-15 1.0167149216103013 1101 16-17 0.8221684247577699 1101 18-19 0.9030945407317787 1101 20-21 0.9306404135866515 1101 22-23 1.0049265606497286 1101 24-25 1.0289142747162714 1101 26-27 0.9897317611294412 1101 28-29 0.9513627941285066 1101 30-31 1.1346950451151798 1101 32-33 1.02697922145898 1101 34-35 1.0047495341923138 1101 36-37 0.890979661722362 1101 38-39 1.02501483302931 1101 40-41 0.980828409792089 1101 42-43 1.0049816007153254 1101 44-45 0.9440156712341121 1101 46-47 0.9361777436176268 1101 48-49 0.8722802189661181 1101 50-51 0.7997514275204054 1101 52-53 0.658476931159484 1101 54-55 0.6227487927181272 1101 56-57 0.6778524519746227 1101 58-59 0.7717909978979094 1101 60-61 0.7658590187968244 1101 62-63 0.8439031327893431 1101 64-65 0.7766050784959262 1101 66-67 0.8592143175429712 1101 68-69 0.7621816434908837 1101 70-71 0.7828446176089656 1101 72-73 0.7843835732289719 1101 74-75 0.7944415181011806 1101 76-77 0.7418248036521362 1101 78-79 0.6675452986724153 1101 80-81 0.7160510099205837 1101 82-83 0.6981883090655359 1101 84-85 0.7593227772550648 1101 86-87 0.6696877935482632 1101 88-89 0.6401145810350428 1101 90-91 0.6746396283993086 1101 92-93 0.77400730183437 1101 94-95 0.7513790757534835 1101 96-97 0.7328785272430274 1101 98-99 0.7539872978267965 1101 100-101 0.7083502159743702 1101 102-103 0.6346319972776371 1101 104-105 0.7054097336019822 1101 106-107 0.603122533126129 1101 108-109 0.6643268336068253 1101 110-111 0.7338251855305558 1101 112-113 0.6825521800081837 1101 114-115 0.7297664734292049 1101 116-117 0.582887239634033 1101 118-119 0.532751735599291 1101 120-121 0.47814025974778573 1101 122-123 0.5548153153630189 1101 124-125 0.2719534736474323 1102 1 0.38887843207510997 1102 2 0.25435191223697373 1102 3 0.4150807860974801 1102 4 0.4034689186738305 1102 5 0.4116264581527034 1102 6 0.2636796424808878 1102 7 0.36332388186442444 1102 8 0.40827525552190025 1102 9 0.7003581751991916 1102 10-11 0.3976455443298619 1102 12-13 0.4162558783710111 1102 14-15 0.6419787464950986 1102 16-17 0.4990716505642183 1102 18-19 0.4271717296718691 1102 20-21 0.5841680633562376 1102 22-23 0.6207572058110244 1102 24-25 0.5835386986794049 1102 26-27 0.4957755399312873 1102 28-29 0.539522932377352 1102 30-31 0.5686858285253251 1102 32-33 0.4438939680027616 1102 34-35 0.33926105492963643 1102 36-37 0.29234256494816435 1102 38-39 0.36215769017216815 1102 40-41 0.32838486140499157 1102 42-43 0.385807637581685 1102 44-45 0.2885951643216771 1102 46-47 0.3391247482259203 1102 48-49 0.37272031113201365 1102 50-51 0.25443691139137314 1102 52-53 0.22322923530694538 1102 54-55 0.12535363142781364 1102 56-57 0.21549415704375008 1102 58-59 0.331211504810355 1102 60-61 0.3249730740963699 1102 62-63 0.3946300913146885 1102 64-65 0.2960025439337173 1102 66-67 0.26264173689781245 1102 68-69 0.23616781860609137 1102 70-71 0.37589185263201585 1102 72-73 0.3444112229985592 1102 74-75 0.3109161724790539 1102 76-77 0.3382936976613564 1102 78-79 0.22353953830375417 1102 80-81 0.32357981176389927 1102 82-83 0.2715638851024025 1102 84-85 0.3922651735684255 1102 86-87 0.37677765529940643 1102 88-89 0.3277044427861995 1102 90-91 0.22483778508133767 1102 92-93 0.25578495160395676 1102 94-95 0.3311751587027949 1102 96-97 0.22609857332597727 1102 98-99 0.23066702132910066 1102 100-101 0.13797348786377128 1102 102-103 0.04056748114859943 1102 104-105 0.20632217599829517 1102 106-107 0.12605801699709573 1102 108-109 0.17522890733954455 1102 110-111 0.11391389520797546 1102 112-113 0.11206139659804748 1102 114-115 0.1444761508485577 1102 116-117 0.08487802304416903 1102 118-119 0.16317800288039663 1102 120-121 0.056705927950549295 1102 122-123 0.08730725084688729 1102 124-125 -0.3279969871820576 1103 1 0.5159528083184348 1103 2 0.5847386737184372 1103 3 0.7808941448725797 1103 4 0.6349636312131182 1103 5 0.5010575429566799 1103 6 0.4592872252447293 1103 7 0.5229656268650658 1103 8 0.6519482553489766 1103 9 1.1064658985483717 1103 10-11 0.8001951963646547 1103 12-13 0.7419898344819842 1103 14-15 0.9393705667385888 1103 16-17 0.8176145924542979 1103 18-19 0.8550307849516088 1103 20-21 0.8114863848597267 1103 22-23 0.8288766772761562 1103 24-25 0.8115124250620624 1103 26-27 0.9550236954912705 1103 28-29 0.9274628473265238 1103 30-31 1.092534018802624 1103 32-33 0.914851628055537 1103 34-35 0.9666189944370203 1103 36-37 0.8784708431276087 1103 38-39 0.9678222561990992 1103 40-41 0.8825035745013423 1103 42-43 0.9016091736579952 1103 44-45 0.7892955746592492 1103 46-47 0.792131706870066 1103 48-49 0.7257734055274767 1103 50-51 0.7087062092039105 1103 52-53 0.6176001868782635 1103 54-55 0.5928903403712837 1103 56-57 0.5784461009495416 1103 58-59 0.6780680777358583 1103 60-61 0.7091488661594312 1103 62-63 0.642063872651029 1103 64-65 0.6880675329706989 1103 66-67 0.49311270932592777 1103 68-69 0.5816420928095383 1103 70-71 0.6336910799406823 1103 72-73 0.5775276170968837 1103 74-75 0.699266169244936 1103 76-77 0.6259908223942148 1103 78-79 0.6101344871980174 1103 80-81 0.6388940348418117 1103 82-83 0.6510348123187981 1103 84-85 0.7322294761133463 1103 86-87 0.6239952371788178 1103 88-89 0.7262831778349792 1103 90-91 0.701756213904897 1103 92-93 0.7089889689784528 1103 94-95 0.6140715912013697 1103 96-97 0.7113449920300319 1103 98-99 0.6401874042228357 1103 100-101 0.6596982537858835 1103 102-103 0.6774888536839043 1103 104-105 0.6230143086316495 1103 106-107 0.544867510005453 1103 108-109 0.5691347887568021 1103 110-111 0.5862468412166066 1103 112-113 0.6398383035094319 1103 114-115 0.58809961579529 1103 116-117 0.529190059129057 1103 118-119 0.5521696265024332 1103 120-121 0.5085502937126734 1103 122-123 0.6054384278563667 1103 124-125 0.42179539368671826 1104 1 0.7399989966564782 1104 2 0.6821222385752321 1104 3 0.7733877856956255 1104 4 0.5994199601067294 1104 5 0.6392034239845188 1104 6 0.6436040074703726 1104 7 0.6782425191034136 1104 8 0.7983441972841163 1104 9 1.256317579930652 1104 10-11 0.9452486634096573 1104 12-13 0.9075867844776511 1104 14-15 1.0247302912915899 1104 16-17 0.8601613061685427 1104 18-19 0.9285852380299531 1104 20-21 0.9458327772818507 1104 22-23 1.0310019529810717 1104 24-25 1.0301378631323814 1104 26-27 0.9596042736678712 1104 28-29 0.945367162353719 1104 30-31 1.0428802902211558 1104 32-33 1.030266553605884 1104 34-35 0.9593122263133296 1104 36-37 0.8769141383442687 1104 38-39 0.9684713934564257 1104 40-41 0.8978431323288945 1104 42-43 0.8428588050511934 1104 44-45 0.721214344587942 1104 46-47 0.7405275252063603 1104 48-49 0.7861807205030829 1104 50-51 0.8336114825276866 1104 52-53 0.6352581963269301 1104 54-55 0.6742634343618761 1104 56-57 0.7325549273022389 1104 58-59 0.69597543407329 1104 60-61 0.76743837488808 1104 62-63 0.772252429019737 1104 64-65 0.7066008720568462 1104 66-67 0.6857351070592372 1104 68-69 0.7242250021707122 1104 70-71 0.7911571883903932 1104 72-73 0.6757511512907755 1104 74-75 0.709621100692992 1104 76-77 0.7356761222090071 1104 78-79 0.6056501360773652 1104 80-81 0.7176195263418705 1104 82-83 0.7384900401770054 1104 84-85 0.7326034761710929 1104 86-87 0.7602221730984908 1104 88-89 0.7484709958546532 1104 90-91 0.6344437054094989 1104 92-93 0.6569608692182882 1104 94-95 0.6734217872888344 1104 96-97 0.6246161101584136 1104 98-99 0.7146426933938557 1104 100-101 0.7085234889075487 1104 102-103 0.6866760031019368 1104 104-105 0.6365962729969006 1104 106-107 0.574221222658565 1104 108-109 0.4952880049383239 1104 110-111 0.6158489680900132 1104 112-113 0.7055845372319318 1104 114-115 0.6380144915492529 1104 116-117 0.5479092425461829 1104 118-119 0.45369009508804936 1104 120-121 0.37410207822375696 1104 122-123 0.4567528659211817 1104 124-125 0.2559052613840471 1105 1 0.4057171113240585 1105 2 0.45120016998037116 1105 3 0.5614515272444152 1105 4 0.35232917818337484 1105 5 0.35276516925443246 1105 6 0.4357910903193627 1105 7 0.4205237438356946 1105 8 0.6196686464578391 1105 9 1.0911956282454724 1105 10-11 0.6730654977030781 1105 12-13 0.7524745079114936 1105 14-15 0.46145718468580554 1105 16-17 0.5920147700393485 1105 18-19 0.5487547984285612 1105 20-21 0.8505784780509842 1105 22-23 0.8644007709014048 1105 24-25 0.7836687803960416 1105 26-27 0.7957411136763071 1105 28-29 0.7708704005990228 1105 30-31 0.9229115987217398 1105 32-33 0.7160556875610169 1105 34-35 0.6435122685077204 1105 36-37 0.7186912179379945 1105 38-39 0.7647637592927055 1105 40-41 0.7195617618572783 1105 42-43 0.8116693540970914 1105 44-45 0.6738412642979767 1105 46-47 0.7506080811923823 1105 48-49 0.5475436155863562 1105 50-51 0.6567647137565018 1105 52-53 0.48457796274941245 1105 54-55 0.5732004043265277 1105 56-57 0.5839473823322763 1105 58-59 0.6944601666050119 1105 60-61 0.6652700090428851 1105 62-63 0.7446596053137355 1105 64-65 0.7041124842680162 1105 66-67 0.5759756469693826 1105 68-69 0.522993067245551 1105 70-71 0.5816490570582502 1105 72-73 0.5610659880324889 1105 74-75 0.5375394990749669 1105 76-77 0.47473071622790286 1105 78-79 0.37958511724103516 1105 80-81 0.4255494645426623 1105 82-83 0.3838259279793519 1105 84-85 0.4932018363808197 1105 86-87 0.546143419125265 1105 88-89 0.4909915528971247 1105 90-91 0.5056415952439295 1105 92-93 0.38443972667280946 1105 94-95 0.38433106868889766 1105 96-97 0.35387820612553966 1105 98-99 0.44557514352984384 1105 100-101 0.2938079288150526 1105 102-103 0.3103778729248887 1105 104-105 0.3903993775629644 1105 106-107 0.3409773880211624 1105 108-109 0.29031760147901764 1105 110-111 0.33270015040832845 1105 112-113 0.3340533641289234 1105 114-115 0.32462710843903864 1105 116-117 0.19747793525479196 1105 118-119 0.10844454658150937 1105 120-121 0.05373814037236002 1105 122-123 0.10229145231962633 1105 124-125 0.011212615460944875 1106 1 0.37888101408336183 1106 2 0.3991894685299968 1106 3 0.4685116459455472 1106 4 0.29215094108094064 1106 5 0.3370496715864846 1106 6 0.25353215345114677 1106 7 0.20955480514863467 1106 8 0.45599915626792864 1106 9 0.8259011177973434 1106 10-11 0.3268449448321036 1106 12-13 0.4879886877008417 1106 14-15 0.7045434605344028 1106 16-17 0.5379236661034597 1106 18-19 0.5724678545624329 1106 20-21 0.7212631992196776 1106 22-23 0.6890986718150174 1106 24-25 0.573968557180109 1106 26-27 0.6674322324442485 1106 28-29 0.6334338163940743 1106 30-31 0.685635074369209 1106 32-33 0.697501243236978 1106 34-35 0.6493051980961013 1106 36-37 0.5793784915614637 1106 38-39 0.7791087709457685 1106 40-41 0.6430718682676257 1106 42-43 0.715289092995512 1106 44-45 0.5365307045511969 1106 46-47 0.659722030957127 1106 48-49 0.5685823480088601 1106 50-51 0.5201856872311197 1106 52-53 0.5147754847105972 1106 54-55 0.4033161582308935 1106 56-57 0.48348515147080917 1106 58-59 0.5595070713580128 1106 60-61 0.583774019609443 1106 62-63 0.581592227539879 1106 64-65 0.5941171864107702 1106 66-67 0.5214400610827141 1106 68-69 0.5492887457174334 1106 70-71 0.5350289179502639 1106 72-73 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-1.0894444760756556 2216 80-81 -1.0432021021126126 2216 82-83 -1.2312959825764338 2216 84-85 -1.3451624035392484 2216 86-87 -1.418817096789624 2216 88-89 -1.830479224536976 2216 90-91 -1.4639433591525588 2216 92-93 -1.3962893786635533 2216 94-95 -1.4176016592791179 2216 96-97 -1.3624623144878534 2216 98-99 -1.3810100347161764 2216 100-101 -1.4137981242745958 2216 102-103 -1.6787131420229002 2216 104-105 -1.4727338645011585 2216 106-107 -1.1539628254986525 2216 108-109 -1.2606445653261922 2216 110-111 -1.3053698352163323 2216 112-113 -1.4943255615448656 2216 114-115 -1.4238756427533694 2216 116-117 -1.335187300970592 2216 118-119 -1.1355855495222258 2216 120-121 -1.1234609554270811 2216 122-123 -1.0752331434395899 2216 124-125 -0.9739530171646571 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32952.0 3 6768.0 4 4281.0 5 3704.0 6 4100.0 7 4768.0 8 5399.0 9 6167.0 10 6709.0 11 7647.0 12 8493.0 13 8651.0 14 8704.0 15 8285.0 16 7836.0 17 7199.0 18 6902.0 19 6807.0 20 6961.0 21 7549.0 22 8542.0 23 11330.0 24 16369.0 25 22776.0 26 27085.0 27 30233.0 28 36601.0 29 43650.0 30 52999.0 31 64602.0 32 81603.0 33 108042.0 34 145708.0 35 214759.0 36 387069.0 37 643276.0 38 254721.0 39 563.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.179406637510006 18.779051401725393 12.401186776978399 24.640355183786202 2 15.663796581874479 20.362723417225013 39.11037473309424 24.863105267806272 3 18.21413882527134 25.089206471527962 29.778120278291286 26.918534424909407 4 12.27861374581992 16.317944170919628 37.693807679052924 33.70963440420753 5 13.05279965087705 38.95868365408427 34.758124988094146 13.230391706944534 6 32.27816535241488 37.00189539727579 17.565804227714363 13.154135022594971 7 28.57773088597369 32.716298187425885 21.477793185497156 17.228177741103266 8 26.521430426876787 35.35271452073773 20.193350073599444 17.932504978786042 9 26.64364310799859 15.779450066845383 19.72813324743875 37.84877357771728 10-11 24.580139517272748 26.906423237031934 28.27754424833841 20.235892997356906 12-13 25.26141745680889 24.18896999627884 28.054235855748864 22.4953766911634 14-15 22.30935634447703 25.774211306428068 25.153140757446295 26.763291591648603 16-17 22.694376832977888 29.03125251648868 25.926609015805063 22.347761634728368 18-19 22.877849794047133 27.8679364315099 27.00463605034663 22.249577724096333 20-21 23.350427009943143 27.248789853431433 26.944385868207366 22.456397268418065 22-23 23.202625910953785 27.153377524275324 26.83003128032859 22.813965284442293 24-25 22.931705858679276 27.351190611495845 26.827136672063727 22.88996685776115 26-27 22.717899791717137 27.659254342581907 26.590692455553437 23.032153410147515 28-29 22.856133426450015 27.75588650560021 26.66517870479775 22.72280136315203 30-31 22.919686030867773 27.26564357220212 26.707099985753867 23.10757041117624 32-33 22.60597242964485 27.457378212098337 26.68554594442245 23.251103413834358 34-35 23.290694407223537 27.373480539080948 26.38224023642048 22.953584817275043 36-37 23.389792351027964 26.991422371105568 26.43665272238247 23.182132555484 38-39 22.81801716462642 27.193877760861994 26.70649969467166 23.28160537983993 40-41 23.101469209506796 27.078796915463144 26.72001807964786 23.0997157953822 42-43 22.883426910653917 27.008780761510202 26.666927876444603 23.440864451391278 44-45 22.83690479489062 27.200977404025988 26.817847667668776 23.14427013341461 46-47 23.26335568029059 27.466300976064872 26.297252774608893 22.973090569035644 48-49 23.044426937983616 27.287694293030878 26.415387616206708 23.2524911527788 50-51 22.996871696702435 27.35067065532012 26.54128630826657 23.11117133971088 52-53 23.235031471388908 27.15439111115922 26.491689713212573 23.118887704239302 54-55 22.846545649145018 27.17547078806289 26.63157603052946 23.34640753226263 56-57 22.779401201338246 27.487875558919306 26.675653827885544 23.057069411856904 58-59 22.917323339210192 27.034876672566615 26.58586419261317 23.461935795610025 60-61 22.659838840025746 27.143879906571293 26.855059722194863 23.341221531208102 62-63 22.71969732068359 27.147438825688774 26.977380886916556 23.155482966711077 64-65 22.710823733265098 27.140277249191318 26.715098897264006 23.433800120279578 66-67 22.6034052303826 27.339721135169164 26.61341862718694 23.443455007261292 68-69 22.779814531524746 26.94469329548392 27.019860983077308 23.25563118991402 70-71 22.75407349071024 26.957634051392798 26.74485938883081 23.54343306906615 72-73 22.75801808476114 27.08999103057852 26.577263999539298 23.574726885121045 74-75 22.754584746694746 27.32932930245689 26.623658912338826 23.292427038509533 76-77 23.002421988923103 27.014961431753083 26.685109400815353 23.297507178508464 78-79 22.96342976964437 27.06242941222697 26.697175233463277 23.276965584665383 80-81 23.01519002195055 27.253709066795352 26.593525594936935 23.137575316317164 82-83 22.98179086284745 27.28045058981323 26.559532869219186 23.178225678120132 84-85 22.914126905673196 27.180882075636113 26.65466849475334 23.25032252393735 86-87 23.12395564825692 27.068644571622556 26.644387162814304 23.163012617306222 88-89 22.96084542633739 27.13521590742779 26.60320888587905 23.300729780355773 90-91 22.90465312230196 27.26860603772343 26.65749025999049 23.16925057998412 92-93 23.060495015702912 26.773816371511387 26.817958147361658 23.347730465424043 94-95 22.82269592289186 26.839037001228977 26.914568269203915 23.42369880667524 96-97 23.07702965500027 26.65486236748577 26.917807136744486 23.35030084076947 98-99 22.963645916294663 26.823933388333604 26.656183276900684 23.556237418471042 100-101 22.78249860525653 26.741188652665905 26.921482383367078 23.554830358710486 102-103 22.67982337638945 26.52586398007572 27.030552351459857 23.763760292074974 104-105 22.633201137609188 26.809370792661262 26.764016235848892 23.793411833880654 106-107 22.839613075626204 26.745203493784047 26.60797799889336 23.80720543169638 108-109 22.854550732581345 26.781934877184415 26.55206086736471 23.81145352286953 110-111 22.862357563927244 27.003456977633018 26.676398188231122 23.45778727020862 112-113 22.848033086134098 26.99960339590095 26.826998347771568 23.325365170193383 114-115 22.832055897583448 27.220130508231293 26.48068778240574 23.467125811779514 116-117 22.77696653356007 27.096006693937465 26.767285004346082 23.359741768156386 118-119 22.702409067183396 27.331976426086808 26.389293246862678 23.576321259867118 120-121 22.65417000836652 27.423324227742548 26.542414615599142 23.380091148291793 122-123 22.682967599503375 27.52110802345235 26.52701614806558 23.268908228978695 124-125 22.73264399849502 27.570348562955143 26.43488589175155 23.26212154679829 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 708.0 1 594.5 2 451.0 3 357.5 4 272.5 5 238.5 6 218.5 7 202.5 8 193.0 9 181.0 10 171.0 11 178.0 12 179.5 13 180.5 14 207.5 15 248.0 16 283.0 17 357.5 18 467.0 19 595.0 20 777.0 21 1079.5 22 1527.0 23 2241.0 24 3375.0 25 5096.0 26 7464.0 27 10746.5 28 14247.5 29 18173.0 30 23092.0 31 28608.0 32 35821.5 33 41065.5 34 43832.0 35 47797.0 36 54441.0 37 61281.0 38 65405.0 39 68236.5 40 69975.0 41 72940.0 42 76503.0 43 79389.0 44 83141.0 45 87228.5 46 88679.5 47 89266.5 48 88915.0 49 87242.0 50 85877.0 51 83520.5 52 82862.0 53 85009.0 54 87482.0 55 82751.0 56 73465.5 57 67259.0 58 62183.0 59 53491.0 60 42132.5 61 33952.0 62 28059.0 63 21337.5 64 15555.0 65 11736.5 66 8536.5 67 6574.0 68 4770.5 69 2959.5 70 2033.0 71 1367.5 72 955.0 73 733.0 74 543.5 75 418.0 76 297.0 77 163.0 78 81.0 79 51.5 80 35.0 81 23.5 82 13.5 83 5.5 84 5.5 85 7.5 86 7.5 87 6.5 88 4.0 89 2.5 90 3.0 91 5.0 92 3.0 93 2.5 94 3.5 95 3.0 96 2.5 97 1.0 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08862200787077724 2 2.5976162541507743E-4 3 0.0 4 0.001991505794848927 5 9.524592931886172E-4 6 1.7317441694338496E-4 7 4.329360423584624E-5 8 4.3293604235846236E-4 9 8.658720847169248E-5 10-11 0.00881024846199471 12-13 0.0020131525969668503 14-15 0.003831483974872392 16-17 0.0019049185863772343 18-19 0.01303137487498972 20-21 0.016408276005385726 22-23 0.009156597295881478 24-25 0.00950294612976825 26-27 0.009113303691645635 28-29 0.007056857490442937 30-31 0.017793671340932803 32-33 0.0020131525969668503 34-35 0.0011689273143678485 36-37 9.741060953065404E-4 38-39 6.494040635376936E-5 40-41 0.0013204549291933104 42-43 0.0019265653884951576 44-45 7.792848762452322E-4 46-47 0.001082340105896156 48-49 0.008074257189985324 50-51 0.003961364787579931 52-53 0.007468146730683476 54-55 0.007446499928565554 56-57 0.009481299327650326 58-59 0.008052610387867401 60-61 0.010455405422956867 62-63 0.013117962083461408 64-65 0.00826907840904663 66-67 0.013161255687697257 68-69 0.013291136500404794 70-71 0.003874777579108238 72-73 0.01283655365592841 74-75 0.013161255687697257 76-77 0.005974517384546781 78-79 0.008030963585749477 80-81 0.013009728072871794 82-83 0.016992739662569648 84-85 0.012620085634749178 86-87 0.015715578337612182 88-89 0.01506617427407449 90-91 0.011429511518263406 92-93 0.008940129274702249 94-95 0.007381559522211784 96-97 0.008637074045051324 98-99 0.005390053727362856 100-101 0.010628579839900251 102-103 0.011516098726735098 104-105 0.009935882172126712 106-107 0.004675709257471394 108-109 0.0028790246816837745 110-111 0.0057796961654854725 112-113 0.00874530805564094 114-115 0.013009728072871794 116-117 0.011148103090730405 118-119 0.21902234382914612 120-121 0.026235924166922817 122-123 0.026712153813517128 124-125 0.23651295994042804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2309810.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.47111562157531 #Duplication Level Percentage of deduplicated Percentage of total 1 84.01172400183877 59.20399915701453 2 9.31664304880378 13.131084589943942 3 3.0266319273758353 6.398703854941614 4 1.3621454728636444 3.83967644446317 5 0.6914447286706369 2.436344071003861 6 0.4041474527771131 1.708843312369265 7 0.2543081917843798 1.2544967390725543 8 0.17225301638281906 0.9711089786942998 9 0.13038679769460437 0.826965278627707 >10 0.5841944576167599 7.11936785881114 >50 0.03524539386752681 1.6916490409585978 >100 0.01060362293695495 1.2660118307559411 >500 1.3594369355335225E-4 0.052498968530394145 >1k 1.3594369355335225E-4 0.09924987481293492 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.329360423584624E-5 0.0 0.0 0.0 0.0 6 4.329360423584624E-5 0.0 0.0 0.0 0.0 7 8.658720847169248E-5 0.0 0.0 0.0 0.0 8 8.658720847169248E-5 0.0 0.0 0.0 0.0 9 8.658720847169248E-5 0.0 0.0 0.0 0.0 10-11 1.082340105896156E-4 0.0 0.0 0.0 0.0 12-13 1.2988081270753871E-4 0.0 0.0 0.0 1.5152761482546185E-4 14-15 1.2988081270753871E-4 0.0 0.0 0.0 1.9482121906130807E-4 16-17 1.2988081270753871E-4 0.0 0.0 2.164680211792312E-5 2.1646802117923118E-4 18-19 1.7317441694338493E-4 0.0 0.0 4.329360423584624E-5 2.5976162541507743E-4 20-21 2.1646802117923118E-4 0.0 0.0 1.082340105896156E-4 2.5976162541507743E-4 22-23 2.1646802117923118E-4 0.0 0.0 3.247020317688468E-4 3.0305522965092365E-4 24-25 3.0305522965092365E-4 2.164680211792312E-5 0.0 4.3293604235846236E-4 3.0305522965092365E-4 26-27 3.0305522965092365E-4 4.329360423584624E-5 0.0 8.225784804810786E-4 3.463488338867699E-4 28-29 3.247020317688468E-4 4.329360423584624E-5 0.0 0.0026192630562686973 3.463488338867699E-4 30-31 3.463488338867699E-4 4.329360423584624E-5 0.0 0.010585286235664406 3.463488338867699E-4 32-33 3.67995636004693E-4 4.329360423584624E-5 0.0 0.02954788489096506 3.8964243812261614E-4 34-35 4.329360423584624E-4 4.329360423584624E-5 0.0 0.06026469709629796 4.7622964659430864E-4 36-37 5.628168550660011E-4 4.329360423584624E-5 0.0 0.10894835505950706 4.7622964659430864E-4 38-39 6.061104593018473E-4 8.658720847169248E-5 0.0 0.18085903169524767 4.7622964659430864E-4 40-41 6.061104593018473E-4 8.658720847169248E-5 0.0 0.2605842038955585 4.7622964659430864E-4 42-43 6.277572614197704E-4 8.658720847169248E-5 0.0 0.3421493542758928 4.978764487122317E-4 44-45 6.494040635376936E-4 8.658720847169248E-5 0.0 0.44142158878868826 5.41170052948078E-4 46-47 6.926976677735397E-4 8.658720847169248E-5 0.0 0.5635961399422463 5.844636571839242E-4 48-49 7.35991272009386E-4 8.658720847169248E-5 0.0 0.6917668552824692 6.061104593018473E-4 50-51 7.35991272009386E-4 8.658720847169248E-5 0.0 0.8372117187127945 6.061104593018473E-4 52-53 7.35991272009386E-4 8.658720847169248E-5 0.0 0.9715734194587433 6.494040635376936E-4 54-55 7.35991272009386E-4 8.658720847169248E-5 0.0 1.118641793047913 6.494040635376936E-4 56-57 7.35991272009386E-4 8.658720847169248E-5 0.0 1.2920110312103592 6.494040635376936E-4 58-59 7.35991272009386E-4 8.658720847169248E-5 0.0 1.4816370177633658 6.494040635376936E-4 60-61 7.35991272009386E-4 8.658720847169248E-5 0.0 1.6870651698624561 6.926976677735398E-4 62-63 7.35991272009386E-4 8.658720847169248E-5 0.0 1.8933591940462635 7.35991272009386E-4 64-65 7.35991272009386E-4 8.658720847169248E-5 0.0 2.1035279958091793 7.35991272009386E-4 66-67 7.792848762452323E-4 8.658720847169248E-5 0.0 2.3432663292651776 7.576380741273091E-4 68-69 7.792848762452323E-4 8.658720847169248E-5 0.0 2.6135699473116834 7.792848762452323E-4 70-71 7.792848762452323E-4 8.658720847169248E-5 0.0 2.9093518514509853 8.009316783631554E-4 72-73 7.792848762452323E-4 8.658720847169248E-5 0.0 3.209484762815989 8.442252825990016E-4 74-75 8.009316783631554E-4 8.658720847169248E-5 0.0 3.509920729410644 8.658720847169247E-4 76-77 8.225784804810786E-4 8.658720847169248E-5 0.0 3.835272165243029 8.658720847169247E-4 78-79 8.225784804810786E-4 8.658720847169248E-5 0.0 4.20244522276724 8.658720847169247E-4 80-81 9.957528974244634E-4 8.658720847169248E-5 0.0 4.605487031400852 8.658720847169247E-4 82-83 9.957528974244634E-4 8.658720847169248E-5 0.0 5.032448556374767 8.658720847169247E-4 84-85 0.001103986908014079 8.658720847169248E-5 0.0 5.446162238452514 8.658720847169247E-4 86-87 0.0012122209186036946 8.658720847169248E-5 0.0 5.872712474186189 8.875188868348479E-4 88-89 0.0012122209186036946 1.2988081270753871E-4 0.0 6.338443421753304 9.09165688952771E-4 90-91 0.0012988081270753871 1.2988081270753871E-4 0.0 6.84064923088912 9.524592931886172E-4 92-93 0.0012988081270753871 1.2988081270753871E-4 0.0 7.36376585087085 0.0010390465016603097 94-95 0.0012988081270753871 1.2988081270753871E-4 0.0 7.88491261185985 0.0010390465016603097 96-97 0.0012988081270753871 1.2988081270753871E-4 0.0 8.420476143059386 0.0010606933037782327 98-99 0.0015152761482546185 1.2988081270753871E-4 0.0 8.982708534468202 0.001082340105896156 100-101 0.0016668037630800801 1.2988081270753871E-4 0.0 9.60351717240812 0.001082340105896156 102-103 0.0017317441694338495 1.2988081270753871E-4 0.0 10.253094410362756 0.001082340105896156 104-105 0.0019482121906130808 1.2988081270753871E-4 0.0 10.907542178793927 0.0011256337101320023 106-107 0.0020780930033206194 1.2988081270753871E-4 0.0 11.553504400794871 0.0011256337101320023 108-109 0.0020780930033206194 1.2988081270753871E-4 0.0 12.229230975707958 0.0011256337101320023 110-111 0.0020780930033206194 1.2988081270753871E-4 0.0 12.942081816253285 0.0011689273143678485 112-113 0.0020997398054385425 1.2988081270753871E-4 0.0 13.698918958702231 0.0011689273143678485 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1975 0.0 45.810863 1 GTATCAA 3270 0.0 45.3256 1 ATCAACG 3205 0.0 43.42514 3 TCAACGC 3260 0.0 43.0574 4 CAACGCA 3295 0.0 42.422283 5 AACGCAG 3555 0.0 39.15235 6 TATCAAC 3745 0.0 37.957664 2 GTACATG 9420 0.0 37.66061 1 TACATGG 9815 0.0 34.723038 2 ACATGGG 9520 0.0 34.424534 3 ACGCAGA 4370 0.0 31.986597 7 CATGGGA 6230 0.0 30.74123 4 CGCAGAG 4580 0.0 30.390089 8 GAGTACA 6925 0.0 24.7551 1 ATGGGAT 2800 0.0 24.642319 5 ATGGGAG 3025 0.0 23.59595 5 CATGGGG 4300 0.0 23.09944 4 GCAGAGT 5850 0.0 23.081339 9 TAAGGTG 1305 0.0 22.78983 5 AGTACAT 7415 0.0 21.657385 2 >>END_MODULE