FastQCFastQC Report
Thu 26 May 2016
SRR1364424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309810
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27060.11715249306219991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25050.10845047861079483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30550.063.575431
GTATCAA53100.050.826081
ATCAACG67250.040.201243
TCAACGC68150.039.6703344
CAACGCA68200.039.6120725
AACGCAG71850.037.5973246
TATCAAC72450.037.1546822
GTACATG92300.034.2748641
ACATGGG93650.032.8744623
TACATGG95950.032.3374862
ACGCAGA85050.031.7621147
CGCAGAG87200.030.908088
CATGGGA63750.029.5177174
GCAGAGT101950.026.0861849
TAAGGTG12650.025.4014975
GTGGTAT17100.025.08531
ATGGGAT26150.025.0309355
AGAGTAC93700.024.60494410-11
GAGTACT56100.024.12730412-13
ATGGGAG32450.024.0221945
Produced by FastQC (version 0.11.5)