FastQCFastQC Report
Thu 26 May 2016
SRR1364423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482572
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.049.0562251
GTATCAA35350.048.6541521
GTACATG108950.040.858821
ATCAACG40200.040.6845363
TCAACGC41050.039.553144
CAACGCA41700.038.7963185
TACATGG116050.037.7698362
AACGCAG43200.037.5869066
ACATGGG113150.037.581513
TATCAAC47000.035.6840362
CATGGGA71700.032.4331444
CATGGGG51200.031.2473954
ACGCAGA52000.030.9972847
CGCAGAG54350.029.766458
TAAGGTG12750.028.9227245
GTAAGGT10250.026.6910344
ATGGGAG31300.025.653535
ATGGGAT35400.023.858485
GAGTACA84350.023.6358471
ATGGGGA31500.023.0359945
Produced by FastQC (version 0.11.5)