##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364423_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2482572 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.646228991545865 31.0 31.0 34.0 27.0 34.0 2 30.70315583999175 31.0 31.0 34.0 27.0 34.0 3 30.650148716734098 31.0 31.0 34.0 26.0 34.0 4 34.24773420468772 37.0 35.0 37.0 30.0 37.0 5 34.4074653222545 37.0 35.0 37.0 30.0 37.0 6 34.50811940197505 37.0 35.0 37.0 31.0 37.0 7 34.3877655914914 37.0 35.0 37.0 30.0 37.0 8 34.241249800610014 37.0 35.0 37.0 30.0 37.0 9 35.33731106288156 39.0 35.0 39.0 28.0 39.0 10-11 35.54681455361617 39.0 35.0 39.0 29.5 39.0 12-13 35.60691371690328 39.0 35.0 39.0 30.0 39.0 14-15 36.740816983354364 40.0 36.0 41.0 30.0 41.0 16-17 36.87529364707247 40.0 36.5 41.0 30.0 41.0 18-19 36.85271565134868 40.0 36.5 41.0 30.0 41.0 20-21 36.795537652080185 40.0 36.5 41.0 30.0 41.0 22-23 36.695356670420836 40.0 36.0 41.0 29.0 41.0 24-25 36.743549431798954 40.0 36.0 41.0 30.0 41.0 26-27 36.743109766806356 40.0 36.0 41.0 30.0 41.0 28-29 36.689507293242656 40.0 36.0 41.0 30.0 41.0 30-31 36.46123214150486 40.0 36.0 41.0 29.0 41.0 32-33 36.53356619667022 40.0 36.0 41.0 30.0 41.0 34-35 36.47760830300189 40.0 36.0 41.0 30.0 41.0 36-37 36.362958858796446 40.0 36.0 41.0 29.0 41.0 38-39 36.221371827282354 40.0 35.0 41.0 28.5 41.0 40-41 36.19559533419373 40.0 35.0 41.0 29.0 41.0 42-43 36.05613774746513 39.5 35.0 41.0 28.0 41.0 44-45 36.0520758310333 39.0 35.0 41.0 28.0 41.0 46-47 35.90040832652588 39.0 35.0 41.0 28.0 41.0 48-49 35.72352725318742 39.0 35.0 41.0 27.5 41.0 50-51 35.588436709992706 39.0 35.0 41.0 27.5 41.0 52-53 35.45858770662039 39.0 34.0 40.5 27.0 41.0 54-55 35.32630614540082 38.5 34.0 40.0 27.0 41.0 56-57 35.27836211799698 38.5 34.0 40.0 27.5 41.0 58-59 35.245522989866956 38.0 34.0 40.0 28.0 41.0 60-61 35.05393197055312 38.0 34.0 40.0 27.5 41.0 62-63 34.78805992333757 37.5 34.0 40.0 27.0 41.0 64-65 34.470476586378965 37.0 34.0 40.0 26.5 41.0 66-67 34.15391396503304 36.5 33.5 39.5 26.0 41.0 68-69 33.85116363191078 36.0 33.0 39.0 26.0 41.0 70-71 33.50403794935252 35.5 33.0 39.0 26.0 41.0 72-73 33.17067017593045 35.0 33.0 38.5 26.0 40.0 74-75 32.819791933527 35.0 33.0 37.0 25.5 39.5 76-77 32.4752343537267 35.0 32.0 37.0 25.0 39.0 78-79 32.16894817149311 35.0 32.0 36.0 25.0 39.0 80-81 31.859924908522288 35.0 32.0 36.0 24.0 37.5 82-83 31.583445313972767 35.0 32.0 35.5 24.0 37.0 84-85 31.257362324234705 35.0 32.0 35.0 22.0 37.0 86-87 31.077628161439023 35.0 32.0 35.0 22.0 36.0 88-89 30.946912113727215 35.0 32.0 35.0 21.0 36.0 90-91 30.82565722162338 35.0 31.0 35.0 20.5 36.0 92-93 30.61324827638433 35.0 31.0 35.0 20.0 35.5 94-95 30.539640542147417 34.0 31.0 35.0 19.5 35.0 96-97 30.468792043090794 34.0 31.0 35.0 19.0 35.0 98-99 30.328017676828708 34.0 31.0 35.0 18.5 35.0 100-101 30.20322431736119 34.0 31.0 35.0 17.5 35.0 102-103 29.169918938906907 33.5 29.5 34.5 15.5 35.0 104-105 30.12508640232791 34.0 31.0 35.0 18.0 35.0 106-107 30.370647054748062 35.0 31.5 35.0 18.0 35.0 108-109 30.368075528121643 35.0 32.0 35.0 17.5 35.0 110-111 30.263440093580368 35.0 32.0 35.0 15.5 35.0 112-113 30.145306359694704 35.0 31.0 35.0 8.5 35.0 114-115 30.04684053473575 35.0 31.0 35.0 4.5 35.0 116-117 29.92614796267742 35.0 31.0 35.0 2.0 35.0 118-119 29.66714057034398 35.0 31.0 35.0 2.0 35.0 120-121 29.534816110066494 35.0 31.0 35.0 2.0 35.0 122-123 29.302804712209756 34.0 31.0 35.0 2.0 35.0 124-125 28.125076734934574 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.656302781629492 1101 2 0.622150737287047 1101 3 0.8184784825775395 1101 4 0.6745411508944699 1101 5 0.5940570705195043 1101 6 0.6167386483471589 1101 7 0.6424011595656296 1101 8 0.7564730445102725 1101 9 1.2480850857215415 1101 10-11 0.976107825590347 1101 12-13 0.825668691765479 1101 14-15 1.0856930512160332 1101 16-17 0.92891535919874 1101 18-19 1.034825987270878 1101 20-21 1.105626967498523 1101 22-23 1.0271419773962904 1101 24-25 1.0452616984148406 1101 26-27 0.9788113008465658 1101 28-29 1.0287250160575994 1101 30-31 1.131723727282676 1101 32-33 0.9718650909505229 1101 34-35 0.9787887702983014 1101 36-37 0.9368588347224289 1101 38-39 1.0620809201866876 1101 40-41 0.9729613858342887 1101 42-43 1.0093347177257286 1101 44-45 0.845683712109917 1101 46-47 0.9329410205797188 1101 48-49 0.8985063450755604 1101 50-51 0.8183111572332464 1101 52-53 0.6988529033070279 1101 54-55 0.6532288015112613 1101 56-57 0.7579187420464208 1101 58-59 0.8320310776136353 1101 60-61 0.6669556603032873 1101 62-63 0.7527919980839286 1101 64-65 0.6796680169543521 1101 66-67 0.7326680181571987 1101 68-69 0.7811087315072953 1101 70-71 0.7458993645555267 1101 72-73 0.6771446867431408 1101 74-75 0.7016718316836545 1101 76-77 0.6234292880069745 1101 78-79 0.536815514414819 1101 80-81 0.5947442251087303 1101 82-83 0.7072204897644134 1101 84-85 0.7452399990582208 1101 86-87 0.7520419589233249 1101 88-89 0.6580749373858801 1101 90-91 0.5951519453263998 1101 92-93 0.7027876812126692 1101 94-95 0.6141983725898754 1101 96-97 0.5764763690161985 1101 98-99 0.590696792619056 1101 100-101 0.6127453626183552 1101 102-103 0.6591808797266197 1101 104-105 0.6082193761777077 1101 106-107 0.6087655321656129 1101 108-109 0.5999845642698709 1101 110-111 0.650975205441199 1101 112-113 0.5939083194633206 1101 114-115 0.6086971150935518 1101 116-117 0.6815594680465331 1101 118-119 0.6609205579244097 1101 120-121 0.5833564946909462 1101 122-123 0.5773843719770042 1101 124-125 0.24674223057229838 1102 1 0.3201400376814476 1102 2 0.23271030290354133 1102 3 0.33811220205836534 1102 4 0.2693703098096023 1102 5 0.21155071918821022 1102 6 0.03897371173077602 1102 7 0.18437614620009413 1102 8 0.22402844123251242 1102 9 0.5710707806357149 1102 10-11 0.3054243171480806 1102 12-13 0.35706545146971536 1102 14-15 0.41787646815301116 1102 16-17 0.2510240444191254 1102 18-19 0.22031281699467797 1102 20-21 0.44605601920117266 1102 22-23 0.407907206541374 1102 24-25 0.30123749778914544 1102 26-27 0.31380918974041805 1102 28-29 0.3046246138666575 1102 30-31 0.30070912822712614 1102 32-33 0.1944098312700575 1102 34-35 0.051050564752934235 1102 36-37 0.009613316680038508 1102 38-39 0.11337193724990868 1102 40-41 0.09373647161148568 1102 42-43 0.17597571007531343 1102 44-45 0.0930200437423565 1102 46-47 0.10581402709749455 1102 48-49 0.07904252280620483 1102 50-51 0.034176300060742903 1102 52-53 -0.025131856379928763 1102 54-55 -0.055357636396990983 1102 56-57 0.005246571613142237 1102 58-59 0.22422344333503474 1102 60-61 0.15609960315787674 1102 62-63 0.10547526443347266 1102 64-65 0.08082379353159297 1102 66-67 0.05344187906052156 1102 68-69 0.06482799617948842 1102 70-71 0.14760619027326527 1102 72-73 0.12331990999441445 1102 74-75 0.15847506938555966 1102 76-77 0.1286761433234318 1102 78-79 -0.0166472760784373 1102 80-81 0.10069250084658066 1102 82-83 -0.003987178234243771 1102 84-85 0.2029987824846664 1102 86-87 0.12235764494635859 1102 88-89 0.12645164806634668 1102 90-91 0.04572950938370113 1102 92-93 0.01848011682393036 1102 94-95 0.08983887354887798 1102 96-97 0.08731960101162883 1102 98-99 -0.0022707021103798297 1102 100-101 -0.08205072470218511 1102 102-103 -0.18876097190801033 1102 104-105 -0.06637544274990503 1102 106-107 -0.16851031951801332 1102 108-109 -0.1481873474988582 1102 110-111 -0.1944591852705102 1102 112-113 -0.2664420809263319 1102 114-115 -0.23744222655835756 1102 116-117 -0.341032898251278 1102 118-119 -0.2751904531153073 1102 120-121 -0.39167690113497144 1102 122-123 -0.36605952663802555 1102 124-125 -0.6156290676066725 1103 1 0.6186414096160817 1103 2 0.6351387554208792 1103 3 0.87501436742102 1103 4 0.7288230745749686 1103 5 0.6088214737280069 1103 6 0.4807212771445748 1103 7 0.6212730442208922 1103 8 0.7709305164958948 1103 9 1.3119246999192455 1103 10-11 0.9455481204738589 1103 12-13 0.890449829504071 1103 14-15 1.0737309107053719 1103 16-17 0.9237538720592227 1103 18-19 0.9607483984433784 1103 20-21 0.9391219794895704 1103 22-23 0.9838870187606332 1103 24-25 0.828639769538249 1103 26-27 0.944385797336615 1103 28-29 0.9178665726802038 1103 30-31 1.1296191072755803 1103 32-33 1.0291905400463506 1103 34-35 1.0455479251908955 1103 36-37 0.9293176801876086 1103 38-39 0.9777881667618544 1103 40-41 0.9960714634644319 1103 42-43 1.131316136300299 1103 44-45 0.9313640399775949 1103 46-47 0.8636132623626196 1103 48-49 0.9058010006275765 1103 50-51 0.9009851156504141 1103 52-53 0.8097858379382075 1103 54-55 0.7680744457347259 1103 56-57 0.8047075700655171 1103 58-59 0.8325570712236683 1103 60-61 0.8989102706340475 1103 62-63 0.8313214525066783 1103 64-65 0.9010604704487832 1103 66-67 0.7608239547120235 1103 68-69 0.8748688319402973 1103 70-71 0.9523826643839328 1103 72-73 0.8246609683004138 1103 74-75 0.9197850919589001 1103 76-77 0.7840747875373637 1103 78-79 0.8071789556998539 1103 80-81 0.7539634161789088 1103 82-83 0.6801595782267391 1103 84-85 0.8301695684205868 1103 86-87 0.8418374533537296 1103 88-89 0.7888536044680059 1103 90-91 0.7725993291089246 1103 92-93 0.7635418798725553 1103 94-95 0.7115657577151815 1103 96-97 0.7971254162956569 1103 98-99 0.775444283407893 1103 100-101 0.7934493089117751 1103 102-103 0.7397593278037427 1103 104-105 0.6558873587967504 1103 106-107 0.7426432075564726 1103 108-109 0.8077815211246282 1103 110-111 0.8865774483990307 1103 112-113 0.9246999480953342 1103 114-115 0.9590234563629103 1103 116-117 0.8151638175610785 1103 118-119 0.7308476533307413 1103 120-121 0.5787020286659157 1103 122-123 0.5717592601683918 1103 124-125 0.32241462083559824 1104 1 0.34489907611068915 1104 2 0.31959855308097573 1104 3 0.4674541155560057 1104 4 0.3252570050467227 1104 5 0.3414189826937104 1104 6 0.27978926980264873 1104 7 0.3751638020327448 1104 8 0.5544873924333871 1104 9 0.957087695352385 1104 10-11 0.5929717238652117 1104 12-13 0.5566819311281748 1104 14-15 0.627296589189136 1104 16-17 0.551728237340086 1104 18-19 0.612575702140397 1104 20-21 0.6334778658417903 1104 22-23 0.6934873699455508 1104 24-25 0.7680657310626984 1104 26-27 0.7121460266558941 1104 28-29 0.6450248213726368 1104 30-31 0.6780039519554037 1104 32-33 0.6073005303430534 1104 34-35 0.5807485026961885 1104 36-37 0.3808740102809409 1104 38-39 0.5674689611916506 1104 40-41 0.521612230796201 1104 42-43 0.5745743675170871 1104 44-45 0.37982269093344456 1104 46-47 0.5107515044099955 1104 48-49 0.4761507130615428 1104 50-51 0.40562458881370844 1104 52-53 0.2881208945641447 1104 54-55 0.2038135993742145 1104 56-57 0.25925657036702887 1104 58-59 0.33707174725813616 1104 60-61 0.441630802195661 1104 62-63 0.3521901821083233 1104 64-65 0.2390999904834743 1104 66-67 0.24317459998937352 1104 68-69 0.22287846943396517 1104 70-71 0.3133056696120633 1104 72-73 0.34248245001922584 1104 74-75 0.3213128083831407 1104 76-77 0.3376518602484424 1104 78-79 0.21723491416607743 1104 80-81 0.3534215701037233 1104 82-83 0.296561656102309 1104 84-85 0.2427794519216917 1104 86-87 0.26597886798548487 1104 88-89 0.31813407182280073 1104 90-91 0.2609955558802106 1104 92-93 0.317689169610631 1104 94-95 0.2575736587016344 1104 96-97 0.2448410153029954 1104 98-99 0.31878499130089466 1104 100-101 0.3684646540368526 1104 102-103 0.2757794131970712 1104 104-105 0.31317485713143967 1104 106-107 0.28087542487580563 1104 108-109 0.23281562964297464 1104 110-111 0.23310883659617687 1104 112-113 0.32251547245363454 1104 114-115 0.3504632363208273 1104 116-117 0.3259397900617458 1104 118-119 0.213872681796083 1104 120-121 0.1238164267082098 1104 122-123 0.14124462944114313 1104 124-125 -0.06433926628145414 1105 1 0.29651692977977007 1105 2 0.34629313862080124 1105 3 0.46468906638276763 1105 4 0.27984038341049455 1105 5 0.4109639951221098 1105 6 0.4528023466425921 1105 7 0.4702378320770535 1105 8 0.6727987509117241 1105 9 1.0131343829131794 1105 10-11 0.6179090414919273 1105 12-13 0.654394294685936 1105 14-15 0.42562226734757047 1105 16-17 0.48191142117235586 1105 18-19 0.48421192822922876 1105 20-21 0.7587421044671032 1105 22-23 0.7659265788507739 1105 24-25 0.8112638830134173 1105 26-27 0.7964338252077141 1105 28-29 0.7333261830931477 1105 30-31 0.8122741886354063 1105 32-33 0.7648448402965826 1105 34-35 0.6720337615311607 1105 36-37 0.7008926528833399 1105 38-39 0.7765735759112005 1105 40-41 0.6772023096895268 1105 42-43 0.7348272584713698 1105 44-45 0.7154079065391912 1105 46-47 0.683436579520766 1105 48-49 0.5653690746739954 1105 50-51 0.6053049627464802 1105 52-53 0.4896331068196247 1105 54-55 0.5773367810450267 1105 56-57 0.526322116676333 1105 58-59 0.6699473011325594 1105 60-61 0.5484340011581565 1105 62-63 0.582332298178784 1105 64-65 0.5996381943092643 1105 66-67 0.5181637926836302 1105 68-69 0.556480544436667 1105 70-71 0.5713962888706803 1105 72-73 0.5804414903304789 1105 74-75 0.6331278953819535 1105 76-77 0.624435065695323 1105 78-79 0.45714629425901876 1105 80-81 0.46682289940233446 1105 82-83 0.49776153722194394 1105 84-85 0.6323082533914857 1105 86-87 0.6062987642353441 1105 88-89 0.530339302558783 1105 90-91 0.5489637824013407 1105 92-93 0.584347010310946 1105 94-95 0.6035949490939423 1105 96-97 0.5358847308547112 1105 98-99 0.5279709309961298 1105 100-101 0.44422305671706397 1105 102-103 0.42432366911407016 1105 104-105 0.3655989358086238 1105 106-107 0.4598041506944597 1105 108-109 0.4758565352951969 1105 110-111 0.5914396482248385 1105 112-113 0.5366581829259118 1105 114-115 0.4845384011322409 1105 116-117 0.40086197746042984 1105 118-119 0.30419609042031226 1105 120-121 0.3050773691052697 1105 122-123 0.3087432785279276 1105 124-125 0.11149667881743497 1106 1 0.37794626049104707 1106 2 0.36613940285498003 1106 3 0.512528926067322 1106 4 0.4296443271248691 1106 5 0.27731054503640706 1106 6 0.23609178710818668 1106 7 0.20762591855176282 1106 8 0.48837954523532545 1106 9 0.8498874622790993 1106 10-11 0.3689581186787052 1106 12-13 0.470667630765341 1106 14-15 0.6317674127786788 1106 16-17 0.5242408435670143 1106 18-19 0.4897685726172796 1106 20-21 0.6393889706226474 1106 22-23 0.5559629532378878 1106 24-25 0.5672472205544423 1106 26-27 0.5915165395674578 1106 28-29 0.49311943041241335 1106 30-31 0.5042808589339742 1106 32-33 0.5331795577390182 1106 34-35 0.45624827408115465 1106 36-37 0.5250844771169909 1106 38-39 0.6264980367730288 1106 40-41 0.6443828130180336 1106 42-43 0.4891727852522223 1106 44-45 0.38590399338594494 1106 46-47 0.4213690527133451 1106 48-49 0.4168105966182125 1106 50-51 0.33972805500123116 1106 52-53 0.38155264468221617 1106 54-55 0.23138390497705075 1106 56-57 0.2246833093576086 1106 58-59 0.31770647279309117 1106 60-61 0.31466793995617337 1106 62-63 0.3680529096671066 1106 64-65 0.3566025773066457 1106 66-67 0.3303492123676506 1106 68-69 0.3221702293912898 1106 70-71 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80-81 -1.4120892992582377 2216 82-83 -1.582337721816618 2216 84-85 -1.598929715700578 2216 86-87 -1.537929897500213 2216 88-89 -2.069916730706762 2216 90-91 -1.856381373498504 2216 92-93 -1.5274018594637866 2216 94-95 -1.5121780407890846 2216 96-97 -1.4434097689315522 2216 98-99 -1.3833026936265895 2216 100-101 -1.5544006916151218 2216 102-103 -1.8011039057916882 2216 104-105 -1.7065619966110255 2216 106-107 -1.416973735751906 2216 108-109 -1.393384886003556 2216 110-111 -1.3719031954089829 2216 112-113 -1.5362005421540808 2216 114-115 -1.4118649888958643 2216 116-117 -1.3451740731632995 2216 118-119 -1.2624141729318907 2216 120-121 -1.311665088503183 2216 122-123 -1.2602281193243243 2216 124-125 -1.1471021333125684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39224.0 3 8056.0 4 5257.0 5 4507.0 6 4876.0 7 5697.0 8 6509.0 9 7212.0 10 7817.0 11 8687.0 12 9379.0 13 9790.0 14 9705.0 15 9327.0 16 8848.0 17 8398.0 18 8036.0 19 7798.0 20 8012.0 21 8504.0 22 10274.0 23 13208.0 24 18668.0 25 25508.0 26 30622.0 27 34618.0 28 41593.0 29 49392.0 30 59848.0 31 72528.0 32 90508.0 33 117709.0 34 159820.0 35 234593.0 36 419791.0 37 672708.0 38 245128.0 39 417.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6055886498289 18.496478066936078 12.362605907945959 24.53532737528906 2 15.853191866460428 20.251272879608145 38.70951759474091 25.18601765919051 3 18.41211453283127 25.086563451130523 29.37759710493794 27.12372491110026 4 12.529168867045554 16.227376378988023 37.155970509082074 34.08748424488435 5 13.496805493399307 38.63679299814746 34.42792330283906 13.438478205614162 6 32.62037535326323 36.536401017011414 17.505381524292588 13.337842105432763 7 29.16265872651428 32.244462597660814 21.38290450387743 17.209974171947483 8 26.934499772612014 34.85110618515465 20.212999503331467 18.001394538901874 9 27.005693321163093 15.562174176796676 19.534528498427832 37.8976040036124 10-11 24.877469172667908 26.6302949904616 27.971613011864438 20.520622825006058 12-13 25.529875098639724 23.92682593768668 27.848125230864294 22.695173732809295 14-15 22.724149911630086 25.4256005881257 24.84955966102207 27.00068983922214 16-17 23.0167715235156 28.63819563373624 25.694906146456926 22.650126696291238 18-19 23.213091506796335 27.544798607697945 26.735764758965765 22.506345126539955 20-21 23.60976482333347 27.002369735074737 26.643263417688168 22.744602023903624 22-23 23.672949215032272 26.742804403739356 26.547098768702167 23.03714761252621 24-25 23.366292040681657 27.046430364122276 26.53210855425094 23.055169040945124 26-27 23.084233268299798 27.309190757476205 26.3695011378519 23.237074836372106 28-29 23.20556131171738 27.169605263369917 26.504009887349316 23.120823537563385 30-31 23.178190417868596 26.892474981064574 26.497570625110793 23.431763975956038 32-33 22.996000227994458 27.16493525823494 26.386068330260848 23.452996183509757 34-35 23.697272270629167 26.917057966011438 26.112579812377724 23.273089950981678 36-37 23.715099219332657 26.67670471012632 26.11883198583073 23.48936408471029 38-39 23.255885147728375 26.841856581283135 26.458905574601733 23.443352696386757 40-41 23.35904233142896 26.635902339912242 26.638339370859317 23.366715957799485 42-43 23.269856922284298 26.593063355162162 26.410182973030782 23.72689674952276 44-45 23.144451415330423 26.77656721573303 26.57095181461785 23.5080295543187 46-47 23.593106158242716 27.08198012958128 26.098892079848234 23.226021632327768 48-49 23.43267188869344 26.93644117042205 26.072286982256266 23.55859995862824 50-51 23.362392026899265 26.809394117981167 26.3898081207776 23.43840573434196 52-53 23.64387404076218 26.614114832391326 26.3054184612354 23.43659266561109 54-55 23.14177464544757 26.73823848724296 26.411653450050455 23.708333417259013 56-57 23.13375745373067 27.14632395418146 26.38612600984404 23.333792582243827 58-59 23.255845806192625 26.527918129406476 26.438829394569236 23.77740666983167 60-61 22.98272089155881 26.63032005749497 26.77713930284123 23.60981974810499 62-63 23.122139798693393 26.730946141851685 26.758360968055584 23.388553091399338 64-65 23.1027437861917 26.715422797187106 26.472105414436175 23.70972800218502 66-67 22.924081046608972 26.97645178158637 26.418484859960373 23.680982311844286 68-69 23.01234606695221 26.48875132541148 26.915703024033057 23.58319958360325 70-71 23.126259834970682 26.488780520030875 26.56690841533506 23.818051229663382 72-73 23.127555023330718 26.740276181718748 26.2560970573987 23.87607173755183 74-75 23.155034638957382 26.86469545655884 26.33118722803136 23.64908267645242 76-77 23.33853388070001 26.623781285361005 26.489014196164966 23.548670637774023 78-79 23.281040867483565 26.673750001258888 26.52248220186295 23.522726929394597 80-81 23.28978765387933 26.86273704073892 26.326745281745662 23.52073002363609 82-83 23.35130855881263 26.83756224517751 26.313755982788905 23.497373213220957 84-85 23.184766877264227 26.76740300275257 26.47647420809375 23.571355911889448 86-87 23.408860080727848 26.597686618184834 26.545514463772985 23.447938837314332 88-89 23.3503781450397 26.67946454380369 26.428274311014597 23.54188300014201 90-91 23.219411697286745 26.949133184800324 26.413374980587378 23.418080137325557 92-93 23.3405550255888 26.560082921892924 26.457115394531606 23.64224665798667 94-95 23.178913059110233 26.402706606447346 26.697767315555836 23.720613018886585 96-97 23.362150801952264 26.267887043257627 26.692444674871396 23.677517479918713 98-99 23.24630848164498 26.453469577086974 26.3855920860888 23.914629855179246 100-101 23.03403953866256 26.413600748820297 26.737009903498947 23.815349809018194 102-103 22.99836903285481 26.209281466013255 26.707313609581878 24.085035891550053 104-105 23.05299062525304 26.432910997713016 26.57807837628464 23.936020000749302 106-107 23.11089098660649 26.341430696935085 26.48242138208335 24.06525693437508 108-109 23.191823661857565 26.30759838798331 26.33049886998105 24.170079080178073 110-111 23.107163675138445 26.55268149882432 26.526819419440628 23.813335406596604 112-113 23.24089650749453 26.594984417953356 26.598710734692695 23.565408339859424 114-115 23.137101498086 26.77976774147804 26.276994836103285 23.80613592433268 116-117 22.940677998534824 26.778804987246662 26.616958564294325 23.663558449924192 118-119 22.958281928224473 26.889965345959073 26.258933992610583 23.89281873320587 120-121 22.895231372245643 27.007886737838287 26.380642478629174 23.716239411286892 122-123 23.067440464762363 27.097566130061985 26.379881625572327 23.455111779603328 124-125 23.068867860534528 27.20408082511257 26.225826275429448 23.50122503892345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 506.0 1 452.0 2 364.0 3 294.5 4 250.0 5 222.0 6 197.0 7 182.5 8 176.5 9 182.0 10 175.5 11 175.0 12 181.0 13 185.0 14 191.5 15 211.5 16 252.5 17 298.0 18 370.5 19 449.5 20 546.0 21 784.0 22 1142.0 23 1605.5 24 2372.5 25 3671.5 26 5615.0 27 8471.5 28 11907.0 29 16354.5 30 21688.5 31 27183.0 32 34611.0 33 40172.5 34 44017.5 35 49417.5 36 57446.5 37 65642.0 38 69936.5 39 72990.5 40 74078.0 41 76463.5 42 80232.5 43 84468.0 44 89550.0 45 93710.0 46 95174.5 47 95194.5 48 94811.5 49 92669.0 50 90350.0 51 88181.0 52 88162.0 53 90196.0 54 94129.5 55 91427.0 56 82099.5 57 75105.5 58 70293.0 59 62599.5 60 51917.5 61 42866.0 62 34953.0 63 26990.0 64 19700.5 65 14553.5 66 10759.0 67 8504.0 68 6543.5 69 4632.5 70 3400.5 71 2267.0 72 1655.0 73 1299.5 74 1007.0 75 734.0 76 463.5 77 273.5 78 166.5 79 107.0 80 73.5 81 47.0 82 24.0 83 13.0 84 12.0 85 10.5 86 11.5 87 10.5 88 7.5 89 5.0 90 6.5 91 6.0 92 4.0 93 4.0 94 3.0 95 1.0 96 0.0 97 1.0 98 2.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08523418454731625 2 4.83369666619941E-4 3 0.0 4 0.0018126362498247783 5 0.0011681433609981905 6 1.6112322220664699E-4 7 0.0 8 0.001248704972101514 9 5.639312777232644E-4 10-11 0.009566691318519664 12-13 0.0021348826942380723 14-15 0.0044107482079069615 16-17 0.0024369887358755356 18-19 0.014178843554184934 20-21 0.017642992831627846 22-23 0.010976519512827826 24-25 0.011036940721155318 26-27 0.01059385186008704 28-29 0.008378407554745643 30-31 0.018206924109351107 32-33 0.002235584708117227 34-35 0.001248704972101514 36-37 6.646332916024187E-4 38-39 3.625272499649557E-4 40-41 0.001732074638721455 42-43 0.003021060416374631 44-45 9.868797360157128E-4 46-47 0.0015105302081873154 48-49 0.008156863124211503 50-51 0.005035100693957718 52-53 0.007955459096453194 54-55 0.008740934804710599 56-57 0.01039244783232873 58-59 0.008680513596383107 60-61 0.010795255887845348 62-63 0.0136350526792375 64-65 0.00876107520748643 66-67 0.013937158720874965 68-69 0.013050980998738406 70-71 0.004189203777372821 72-73 0.013272525429272545 74-75 0.013030840595962575 76-77 0.005679593582784306 78-79 0.008841636818589753 80-81 0.014138562748633272 82-83 0.018206924109351107 84-85 0.01407814154030578 86-87 0.016313726248423005 88-89 0.015689373762372248 90-91 0.011902978040516045 92-93 0.009506270110192172 94-95 0.00811658231865984 96-97 0.0090027600407964 98-99 0.006384507679938386 100-101 0.010775115485069516 102-103 0.012184943679377678 104-105 0.010674413471190363 106-107 0.005578891568905151 108-109 0.0036856937079770497 110-111 0.006968579360437483 112-113 0.009184023665778878 114-115 0.013614912276461668 116-117 0.01129876595724112 118-119 0.22218892342296617 120-121 0.027552070997336635 122-123 0.028256985094490712 124-125 0.23830124564363087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2482572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.73775292941916 #Duplication Level Percentage of deduplicated Percentage of total 1 84.74047775254293 54.01167634112522 2 8.621432213863052 10.99021432689877 3 2.7602615334422835 5.277986029173717 4 1.2558107731632044 3.201702271439167 5 0.6599792242916149 2.1032796368224327 6 0.4156661919887635 1.5896177427655387 7 0.27560816190442067 1.2296651450156724 8 0.1837312073367778 0.9368491438924342 9 0.15508097400694842 0.8896061524777698 >10 0.7874982131498797 9.548330142171512 >50 0.09221474934881872 4.076662500459733 >100 0.05092893598006705 5.604681928896685 >500 0.0013100689811922437 0.539728638861391 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.0280805551661746E-5 2 0.0 0.0 0.0 0.0 4.0280805551661746E-5 3 0.0 0.0 0.0 0.0 4.0280805551661746E-5 4 0.0 0.0 0.0 0.0 4.0280805551661746E-5 5 0.0 0.0 0.0 0.0 4.0280805551661746E-5 6 4.0280805551661746E-5 0.0 0.0 0.0 4.0280805551661746E-5 7 4.0280805551661746E-5 0.0 0.0 0.0 4.0280805551661746E-5 8 4.0280805551661746E-5 0.0 0.0 0.0 4.0280805551661746E-5 9 4.0280805551661746E-5 0.0 0.0 0.0 4.0280805551661746E-5 10-11 4.0280805551661746E-5 0.0 0.0 0.0 4.0280805551661746E-5 12-13 4.0280805551661746E-5 0.0 0.0 0.0 3.826676527407866E-4 14-15 8.056161110332349E-5 0.0 0.0 0.0 5.437908749474336E-4 16-17 8.056161110332349E-5 0.0 0.0 0.0 6.24352486050757E-4 18-19 1.2084241665498525E-4 0.0 0.0 0.0 6.444928888265879E-4 20-21 1.6112322220664699E-4 0.0 0.0 4.0280805551661746E-5 6.444928888265879E-4 22-23 2.0140402775830872E-4 0.0 0.0 8.056161110332349E-5 6.847736943782496E-4 24-25 3.625272499649557E-4 0.0 0.0 2.4168483330997046E-4 6.847736943782496E-4 26-27 3.625272499649557E-4 0.0 0.0 5.840716804990953E-4 6.847736943782496E-4 28-29 5.035100693957719E-4 0.0 0.0 0.0029404988052713073 6.847736943782496E-4 30-31 6.042120832749261E-4 0.0 0.0 0.010895957901724502 6.847736943782496E-4 32-33 6.24352486050757E-4 0.0 0.0 0.028277125497266548 7.250544999299114E-4 34-35 7.85475708257404E-4 0.0 0.0 0.05840716804990953 8.056161110332349E-4 36-37 9.063181249123893E-4 0.0 0.0 0.1079726992812293 8.056161110332349E-4 38-39 0.0010875817498948671 0.0 0.0 0.18053857048254793 8.056161110332349E-4 40-41 0.001107722152670698 0.0 0.0 0.25654845055853365 8.056161110332349E-4 42-43 0.0011882837637740215 0.0 0.0 0.33876157468947526 8.056161110332349E-4 44-45 0.0013695473887564993 0.0 0.0 0.43386455659694867 8.861777221365584E-4 46-47 0.0015105302081873154 0.0 0.0 0.5541430419742106 9.667393332398819E-4 48-49 0.001570951416514808 0.0 0.0 0.6807254734203076 9.667393332398819E-4 50-51 0.001570951416514808 0.0 0.0 0.8182844243792324 0.0010473009443432053 52-53 0.001570951416514808 0.0 0.0 0.9510902402830612 0.0010473009443432053 54-55 0.001570951416514808 0.0 0.0 1.0930599394498932 0.0010875817498948671 56-57 0.0016515130276181316 0.0 0.0 1.2662674033220385 0.001107722152670698 58-59 0.0016515130276181316 0.0 0.0 1.4573394044563461 0.001127862555446529 60-61 0.0016515130276181316 0.0 0.0 1.660394945242273 0.001127862555446529 62-63 0.0016515130276181316 0.0 0.0 1.8596640903063437 0.0011681433609981905 64-65 0.0016515130276181316 0.0 0.0 2.069365964008295 0.0012084241665498523 66-67 0.0018126362498247786 0.0 0.0 2.3128231527625385 0.0012084241665498523 68-69 0.0018126362498247786 0.0 0.0 2.583892833722446 0.0012084241665498523 70-71 0.001913338263703933 0.0 0.0 2.873149298388929 0.001248704972101514 72-73 0.002034180680358918 0.0 0.0 3.172677368471086 0.0013695473887564993 74-75 0.002054321083134749 0.0 0.0 3.4666668277898887 0.0014903898054114846 76-77 0.002054321083134749 0.0 0.0 3.7916926477862476 0.0014903898054114846 78-79 0.0020744614859105797 0.0 0.0 4.158610505556334 0.0015105302081873154 80-81 0.002336286721996381 0.0 0.0 4.558196096628819 0.0015306706109631462 82-83 0.002376567527548043 0.0 0.0 4.9699666313806805 0.0016313726248423006 84-85 0.002477269541427197 0.0 0.0 5.380065512702149 0.0016716534303939624 86-87 0.0025376907497546898 0.0 0.0 5.816548321659956 0.0018327766526006093 88-89 0.0025376907497546898 0.0 0.0 6.2802005339623586 0.0019334786664797637 90-91 0.0027592351802888295 0.0 0.0 6.782743864024891 0.0019334786664797637 92-93 0.0029002179997196457 0.0 0.0 7.306595740224251 0.0019737594720314255 94-95 0.0029002179997196457 0.0 0.0 7.8388663047839096 0.0019737594720314255 96-97 0.0029002179997196457 0.0 0.0 8.376212250843079 0.002497409944203028 98-99 0.003061341221926293 0.0 0.0 8.93696134492776 0.002658533166409675 100-101 0.003262745249684601 0.0 0.0 9.556459993909542 0.002658533166409675 102-103 0.0035044300829945715 0.0 0.0 10.212392631512802 0.0026988139719613365 104-105 0.003947518944062851 0.0 0.0 10.870862959865818 0.0027390947775129985 106-107 0.003947518944062851 0.0 0.0 11.516020481984008 0.0027390947775129985 108-109 0.003947518944062851 0.0 0.0 12.191972679946442 0.0027793755830646605 110-111 0.003967659346838682 0.0 0.0 12.909837056085383 0.0027793755830646605 112-113 0.004028080555166174 0.0 0.0 13.659865655457324 0.0027793755830646605 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2305 0.0 49.056225 1 GTATCAA 3535 0.0 48.654152 1 GTACATG 10895 0.0 40.85882 1 ATCAACG 4020 0.0 40.684536 3 TCAACGC 4105 0.0 39.55314 4 CAACGCA 4170 0.0 38.796318 5 TACATGG 11605 0.0 37.769836 2 AACGCAG 4320 0.0 37.586906 6 ACATGGG 11315 0.0 37.58151 3 TATCAAC 4700 0.0 35.684036 2 CATGGGA 7170 0.0 32.433144 4 CATGGGG 5120 0.0 31.247395 4 ACGCAGA 5200 0.0 30.997284 7 CGCAGAG 5435 0.0 29.76645 8 TAAGGTG 1275 0.0 28.922724 5 GTAAGGT 1025 0.0 26.691034 4 ATGGGAG 3130 0.0 25.65353 5 ATGGGAT 3540 0.0 23.85848 5 GAGTACA 8435 0.0 23.635847 1 ATGGGGA 3150 0.0 23.035994 5 >>END_MODULE