FastQCFastQC Report
Thu 26 May 2016
SRR1364423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482572
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.10094369871246434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.065.54111
GTATCAA53050.050.5415081
ATCAACG67400.039.498883
TCAACGC69000.038.4095574
CAACGCA69250.038.3244445
AACGCAG71550.037.255076
GTACATG105850.036.926151
TATCAAC72450.036.4997942
TACATGG111350.034.125292
ACATGGG108100.034.1037453
ACGCAGA84950.031.0982997
CGCAGAG88100.029.9833648
CATGGGG52350.029.2378084
TAAGGTG11250.028.033945
CATGGGA67350.026.7053174
GCAGAGT109100.023.9939219
GTACCGT2052.2648728E-623.2195156
TATACCG2102.7898404E-622.6689535
CAGAGTA101800.022.55863610-11
GAGTACT50750.022.44946712-13
Produced by FastQC (version 0.11.5)