FastQCFastQC Report
Thu 26 May 2016
SRR1364422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186789
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27400.050.1770551
CAACGCA28100.046.9928785
ATCAACG28400.046.6984443
TCAACGC29000.045.5282334
AACGCAG30350.043.313076
GGTATCA15650.042.9742051
TATCAAC33750.039.824522
ACGCAGA36150.036.1984377
GTACATG80100.035.665841
ACATGGG78050.034.8986363
TACATGG82050.034.791932
CGCAGAG38050.034.3908968
CATGGGG45050.028.91184
CATGGGA36200.026.7796464
GAGTACA54050.024.5557331
TAAGGTG14200.024.2954565
GCAGAGT55500.023.1497449
GTAAGGT13750.022.9244544
AGAGTAC42450.021.71897910-11
GTATAAC6950.020.552761
Produced by FastQC (version 0.11.5)