FastQCFastQC Report
Thu 26 May 2016
SRR1364422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186789
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22900.10471975119684615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28250.066.014381
GTATCAA50300.050.9346281
ATCAACG62950.040.2028923
CAACGCA63200.039.9194955
TCAACGC64000.039.5433124
AACGCAG66050.038.37636
TATCAAC66550.037.5824622
ACGCAGA76950.032.940287
CGCAGAG78000.032.4168628
TACATGG77350.030.4103072
GTACATG79250.030.2983111
ACATGGG76650.029.5213053
CATGGGG43700.026.162784
TGGTATC14050.025.4307372
GCAGAGT99000.025.2400829
AGAGTAC85050.024.69318210-11
CAGAGTA86650.024.64918510-11
TAAGGTG13200.023.8912125
GAGTACT57300.023.25787712-13
GTGGTAT18150.022.9791551
Produced by FastQC (version 0.11.5)