FastQCFastQC Report
Thu 26 May 2016
SRR1364421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618681
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.053.5734021
GTATCAA31200.048.651061
CAACGCA34300.041.273785
ATCAACG35250.040.153773
TCAACGC35850.039.4832464
AACGCAG36500.038.1327256
TATCAAC40200.036.8367922
GTACATG90450.035.1430441
TACATGG89750.034.3244672
ACATGGG93350.031.4080583
ACGCAGA45700.030.4561147
CGCAGAG48050.028.8427968
CATGGGG66500.026.651384
GAGTACA71450.022.4107691
ATGGGGA39100.022.0588425
TAAGGTG14100.021.5150575
CATGGGA40650.020.4829544
AGTACAT73900.020.118892
GCAGAGT65400.020.1000739
CCGTTGC3852.3283064E-1020.084729
Produced by FastQC (version 0.11.5)