FastQCFastQC Report
Thu 26 May 2016
SRR1364421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618681
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.064.3904341
GTATCAA53200.052.2971461
ATCAACG66700.040.8958783
CAACGCA66900.040.73785
TCAACGC69350.039.3331644
AACGCAG70500.038.736066
TATCAAC71050.038.1420332
ACGCAGA81400.033.4759527
GTACATG86350.033.1860431
CGCAGAG85100.031.9493458
TACATGG88200.031.8059832
ACATGGG92000.029.908463
TAAGGTG13950.026.4469575
GCAGAGT105300.025.6509119
GTGGTAT18100.024.0279671
CAGAGTA95300.023.8476610-11
AGAGTAC93050.023.7849310-11
CATGGGA42500.023.5429174
CATGGGG61600.023.1077214
GAGTACT59800.022.78293212-13
Produced by FastQC (version 0.11.5)