FastQCFastQC Report
Thu 26 May 2016
SRR1364420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124992
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23300.042.9226071
ATCAACG22000.041.900833
CAACGCA22850.040.087545
TCAACGC23650.038.9784284
AACGCAG25500.036.3880966
GTACATG61950.034.8817371
TACATGG63900.033.505432
GGTATCA15500.033.0293121
TATCAAC29400.032.3657882
ACATGGG62700.032.3444943
ACGCAGA30950.029.7883137
CGCAGAG31600.028.6108918
CATGGGG43350.027.5759664
TAAGGTG12600.026.9078625
GTACCGT1451.3435785E-424.6126376
GTAAGGT12100.023.592814
GGTAAGG12600.022.6560523
GAGTACA47850.022.269161
GTATAGA8300.020.7994881
GTGTAAG11300.020.5456051
Produced by FastQC (version 0.11.5)