##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364420_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2124992 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.73191099072373 31.0 31.0 34.0 27.0 34.0 2 30.74439809655754 31.0 31.0 34.0 27.0 34.0 3 30.696375327530646 31.0 31.0 34.0 27.0 34.0 4 34.28084435141403 37.0 35.0 37.0 30.0 37.0 5 34.43521669728639 37.0 35.0 37.0 31.0 37.0 6 34.5796737117128 37.0 35.0 37.0 31.0 37.0 7 34.428541378038126 37.0 35.0 37.0 30.0 37.0 8 34.29257663087673 37.0 35.0 37.0 30.0 37.0 9 35.451017227358975 39.0 35.0 39.0 29.0 39.0 10-11 35.64988856428636 39.0 35.0 39.0 29.5 39.0 12-13 35.703355118513386 39.0 35.0 39.0 30.0 39.0 14-15 36.85719358002289 40.0 37.0 41.0 30.0 41.0 16-17 36.99090820106617 40.0 37.0 41.0 30.5 41.0 18-19 36.97024200561696 40.0 37.0 41.0 30.0 41.0 20-21 36.93289057088215 40.0 37.0 41.0 30.0 41.0 22-23 36.84187893413245 40.0 37.0 41.0 30.0 41.0 24-25 36.88539509795802 40.0 36.5 41.0 30.0 41.0 26-27 36.89494807509864 40.0 36.5 41.0 30.0 41.0 28-29 36.84599777316809 40.0 36.0 41.0 30.0 41.0 30-31 36.6244632920971 40.0 36.0 41.0 30.0 41.0 32-33 36.70456006422613 40.0 36.0 41.0 30.0 41.0 34-35 36.65697400272566 40.0 36.0 41.0 30.0 41.0 36-37 36.53292247688462 40.0 36.0 41.0 30.0 41.0 38-39 36.39138853228624 40.0 36.0 41.0 29.5 41.0 40-41 36.37083151371864 40.0 36.0 41.0 29.0 41.0 42-43 36.23151169510285 40.0 35.0 41.0 29.0 41.0 44-45 36.2101356146282 40.0 35.0 41.0 29.0 41.0 46-47 36.07058967751408 39.0 35.0 41.0 28.5 41.0 48-49 35.896610904888114 39.0 35.0 41.0 28.0 41.0 50-51 35.770613254073425 39.0 35.0 41.0 28.0 41.0 52-53 35.65453846414481 39.0 35.0 41.0 28.0 41.0 54-55 35.53284577071349 39.0 34.5 40.0 28.0 41.0 56-57 35.49734799001597 39.0 34.5 40.5 28.0 41.0 58-59 35.48864066311779 39.0 35.0 41.0 28.0 41.0 60-61 35.31620566100955 38.0 34.0 40.5 28.0 41.0 62-63 35.064632243321384 38.0 34.0 40.0 28.0 41.0 64-65 34.76989419254284 37.0 34.0 40.0 27.5 41.0 66-67 34.47317519313014 37.0 34.0 40.0 27.0 41.0 68-69 34.17024299385598 36.5 34.0 39.5 26.0 41.0 70-71 33.82133015088998 36.0 33.0 39.0 26.0 41.0 72-73 33.488577604056864 35.5 33.0 39.0 26.0 40.5 74-75 33.12982142991597 35.0 33.0 37.5 26.0 40.0 76-77 32.77782904594464 35.0 33.0 37.0 26.0 39.0 78-79 32.45074922635003 35.0 32.5 37.0 25.5 39.0 80-81 32.125971533069304 35.0 32.0 36.0 25.0 38.5 82-83 31.83230501573653 35.0 32.0 36.0 25.0 37.0 84-85 31.48861689832244 35.0 32.0 35.0 23.5 37.0 86-87 31.29629593899648 35.0 32.0 35.0 23.0 36.5 88-89 31.150720802713607 35.0 32.0 35.0 23.0 36.0 90-91 31.020553959732553 35.0 32.0 35.0 23.0 36.0 92-93 30.79883641914887 35.0 31.5 35.0 20.0 36.0 94-95 30.719531650001507 34.5 31.0 35.0 20.0 35.0 96-97 30.64763161461314 34.5 31.0 35.0 20.0 35.0 98-99 30.498513170873114 34.0 31.0 35.0 19.5 35.0 100-101 30.368800447248745 34.0 31.0 35.0 18.5 35.0 102-103 29.30925669367226 33.5 29.5 34.5 17.5 35.0 104-105 30.272906674472186 34.0 31.0 35.0 19.0 35.0 106-107 30.525195388970875 35.0 32.0 35.0 19.0 35.0 108-109 30.52515280057525 35.0 32.0 35.0 19.0 35.0 110-111 30.42008346384363 35.0 32.0 35.0 18.0 35.0 112-113 30.30407902712104 35.0 32.0 35.0 15.0 35.0 114-115 30.208043136162395 35.0 32.0 35.0 10.0 35.0 116-117 30.09572224271903 35.0 32.0 35.0 4.5 35.0 118-119 29.840497281872118 35.0 31.0 35.0 2.0 35.0 120-121 29.71276833042195 35.0 31.0 35.0 2.0 35.0 122-123 29.482576875583533 35.0 31.0 35.0 2.0 35.0 124-125 28.318478375448002 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6694478166073203 1101 2 0.6788731220282358 1101 3 0.8442633799259767 1101 4 0.735392699296959 1101 5 0.6685214067851035 1101 6 0.6331814939003237 1101 7 0.7300270539492644 1101 8 0.7764506594611902 1101 9 1.2684238599098592 1101 10-11 1.0704787107467837 1101 12-13 0.8238348880850026 1101 14-15 1.0638742302729867 1101 16-17 0.8689705875067304 1101 18-19 0.9130425992030311 1101 20-21 0.940361556445481 1101 22-23 0.7869953611487901 1101 24-25 0.9363075127108971 1101 26-27 0.8858643280790517 1101 28-29 0.8840382701012572 1101 30-31 1.030866181284999 1101 32-33 0.9248259264887935 1101 34-35 0.8972359013569573 1101 36-37 0.8802917863496091 1101 38-39 0.9691028754934621 1101 40-41 0.9419315435804734 1101 42-43 0.8745130789079383 1101 44-45 0.6985251789240507 1101 46-47 0.7617556379760941 1101 48-49 0.7147303876493112 1101 50-51 0.6557502987425536 1101 52-53 0.5034852086177679 1101 54-55 0.6323427918488065 1101 56-57 0.5514716123196024 1101 58-59 0.6043717455173052 1101 60-61 0.6682895979095207 1101 62-63 0.7529687504101545 1101 64-65 0.6373050621354182 1101 66-67 0.6595726935358925 1101 68-69 0.6927381662065955 1101 70-71 0.5858271952003946 1101 72-73 0.5902966355826607 1101 74-75 0.6762559128595313 1101 76-77 0.5787002529666694 1101 78-79 0.4330301994823458 1101 80-81 0.4836233335699802 1101 82-83 0.5350516932365359 1101 84-85 0.7127618986430768 1101 86-87 0.6838036192538475 1101 88-89 0.6606958218814114 1101 90-91 0.6514519460940775 1101 92-93 0.6612773835228118 1101 94-95 0.5614905889122639 1101 96-97 0.5192970923375135 1101 98-99 0.5369239799773737 1101 100-101 0.49530787748349425 1101 102-103 0.476689090467886 1101 104-105 0.5360603358451286 1101 106-107 0.44399309148369426 1101 108-109 0.5182641823535334 1101 110-111 0.5245290257302564 1101 112-113 0.3759495984785559 1101 114-115 0.4433874848592154 1101 116-117 0.4589236444402154 1101 118-119 0.4021826798184165 1101 120-121 0.2564844996519966 1101 122-123 0.32927236607811494 1101 124-125 0.01997899649786561 1102 1 0.3116134390406984 1102 2 0.15839584714372634 1102 3 0.34999770720937207 1102 4 0.28433304503531787 1102 5 0.25665756607232737 1102 6 0.18805384824566573 1102 7 0.3038990741190375 1102 8 0.3644560117614688 1102 9 0.699288739234035 1102 10-11 0.3853276473075482 1102 12-13 0.3697211260133031 1102 14-15 0.48495996356479054 1102 16-17 0.2771632526667318 1102 18-19 0.3150420830351308 1102 20-21 0.423945867922221 1102 22-23 0.4298397777104057 1102 24-25 0.5015102360849113 1102 26-27 0.32956878587134497 1102 28-29 0.3040341576237182 1102 30-31 0.38724025671297824 1102 32-33 0.3452568403962246 1102 34-35 0.1843613560436097 1102 36-37 0.14899811701555166 1102 38-39 0.23713580512283983 1102 40-41 0.225429114531984 1102 42-43 0.27861116179132495 1102 44-45 0.19082276540767396 1102 46-47 0.2082151321315493 1102 48-49 0.2127188134862621 1102 50-51 0.1810151191545586 1102 52-53 0.20522105851802053 1102 54-55 0.022559547066762775 1102 56-57 0.12953494721922 1102 58-59 0.35181394922476983 1102 60-61 0.32472847868807264 1102 62-63 0.2877056538263574 1102 64-65 0.3809779360218002 1102 66-67 0.2817824059644778 1102 68-69 0.27116558382172684 1102 70-71 0.32800206433456935 1102 72-73 0.3474002198117674 1102 74-75 0.3295318434325907 1102 76-77 0.35402449544320547 1102 78-79 0.22325182809404964 1102 80-81 0.3054457827945072 1102 82-83 0.17062176067567236 1102 84-85 0.4043872705702931 1102 86-87 0.32380357218686484 1102 88-89 0.3050279963210585 1102 90-91 0.25657104909153716 1102 92-93 0.16249563390189792 1102 94-95 0.3064637862272477 1102 96-97 0.19221191718166608 1102 98-99 0.2303508406332213 1102 100-101 0.1718404372448319 1102 102-103 0.16619144734699987 1102 104-105 0.15795891591273303 1102 106-107 0.16566886822617022 1102 108-109 0.20068840059057536 1102 110-111 0.14614579224429392 1102 112-113 0.12387095386642955 1102 114-115 0.12744835933235876 1102 116-117 0.19449359421190238 1102 118-119 0.2928515722303544 1102 120-121 0.1605521360815274 1102 122-123 0.01893592736991323 1102 124-125 -0.2968993114284473 1103 1 0.5476493011276702 1103 2 0.5457107507807564 1103 3 0.7184069927119126 1103 4 0.6480409138488454 1103 5 0.5654107836835252 1103 6 0.5058043460298336 1103 7 0.5850465198401267 1103 8 0.6740526787239602 1103 9 1.2374533611026806 1103 10-11 0.8744000683160067 1103 12-13 0.740641421997978 1103 14-15 0.9727361340456042 1103 16-17 0.8100644018250733 1103 18-19 0.7597648304783604 1103 20-21 0.8130871248409974 1103 22-23 0.876463111330473 1103 24-25 0.7567721744538787 1103 26-27 0.7848053272380184 1103 28-29 0.7228961907224374 1103 30-31 0.9187392614368974 1103 32-33 0.7692258433890231 1103 34-35 0.7122612830869031 1103 36-37 0.7878956572082316 1103 38-39 0.7928380945841695 1103 40-41 0.7422752569202231 1103 42-43 0.7740903589966592 1103 44-45 0.5462489903308168 1103 46-47 0.5354958866884729 1103 48-49 0.5316245972322022 1103 50-51 0.5269428944443817 1103 52-53 0.45982181754450835 1103 54-55 0.4362697003440559 1103 56-57 0.5068124333855479 1103 58-59 0.5833874109063188 1103 60-61 0.4908758235810211 1103 62-63 0.5195133095758777 1103 64-65 0.5368751501547635 1103 66-67 0.4140168131841264 1103 68-69 0.49427556998732314 1103 70-71 0.5802222562470689 1103 72-73 0.5369708682419514 1103 74-75 0.533534798720126 1103 76-77 0.5215845546775526 1103 78-79 0.42387923244345416 1103 80-81 0.49857357024215787 1103 82-83 0.49801639745616555 1103 84-85 0.6264779182873745 1103 86-87 0.5954421444954008 1103 88-89 0.6559279143044243 1103 90-91 0.610298538964642 1103 92-93 0.527545052844399 1103 94-95 0.5050872433739713 1103 96-97 0.6068162261381929 1103 98-99 0.5439990454879542 1103 100-101 0.49224685552615455 1103 102-103 0.5038674862251646 1103 104-105 0.5248598221093381 1103 106-107 0.5206224185994763 1103 108-109 0.5358637553473429 1103 110-111 0.5230925426116322 1103 112-113 0.5196902487883648 1103 114-115 0.5103963814007635 1103 116-117 0.47684406095853404 1103 118-119 0.3454589734448348 1103 120-121 0.2681177442734892 1103 122-123 0.26548353759188004 1103 124-125 0.021799555428817285 1104 1 0.5789138902292343 1104 2 0.5978963041227487 1104 3 0.7207126485454722 1104 4 0.593905620972869 1104 5 0.6064129962194826 1104 6 0.5953586070875048 1104 7 0.6516075864488613 1104 8 0.753246077632177 1104 9 1.1640346463765283 1104 10-11 0.9920375313569494 1104 12-13 0.8560983680666467 1104 14-15 0.9650413218723486 1104 16-17 0.8890463309838665 1104 18-19 0.9312259188888916 1104 20-21 0.9892280407322787 1104 22-23 0.9298766529441806 1104 24-25 1.0003436288881176 1104 26-27 0.9285518412612674 1104 28-29 0.8500397775267174 1104 30-31 0.9998166285499224 1104 32-33 0.8633777899892436 1104 34-35 0.8770279479816878 1104 36-37 0.8537462875344559 1104 38-39 0.9767466237099924 1104 40-41 0.8543853282729685 1104 42-43 0.7200159262671519 1104 44-45 0.7089996147250943 1104 46-47 0.704478898233404 1104 48-49 0.7054466163561557 1104 50-51 0.6916962702565499 1104 52-53 0.5938679271944594 1104 54-55 0.5083132357638931 1104 56-57 0.549618735184076 1104 58-59 0.6121888011369592 1104 60-61 0.6759232656059524 1104 62-63 0.7798341118820957 1104 64-65 0.5872901739826659 1104 66-67 0.5235015684878093 1104 68-69 0.6785948088040783 1104 70-71 0.7172713615258743 1104 72-73 0.6134174715524381 1104 74-75 0.6625999098322666 1104 76-77 0.6510481456727106 1104 78-79 0.605895222869151 1104 80-81 0.7487514802431008 1104 82-83 0.6482698915219167 1104 84-85 0.6743396085970481 1104 86-87 0.7325471987379686 1104 88-89 0.8056160927217242 1104 90-91 0.694427917235874 1104 92-93 0.7088361141837218 1104 94-95 0.6892445094591508 1104 96-97 0.6448270950670079 1104 98-99 0.7492220594056676 1104 100-101 0.6900177756237156 1104 102-103 0.6120476002878625 1104 104-105 0.6084020251541951 1104 106-107 0.6418651712345635 1104 108-109 0.4961075869018643 1104 110-111 0.5851691697731347 1104 112-113 0.720856144303049 1104 114-115 0.7053584901817267 1104 116-117 0.6379529058637701 1104 118-119 0.48200993072101284 1104 120-121 0.4612124962029114 1104 122-123 0.6155079586886245 1104 124-125 0.4627580873280053 1105 1 0.11451311632099959 1105 2 0.058303435134359205 1105 3 0.23875825385227145 1105 4 0.1290316574571122 1105 5 0.07608850755170948 1105 6 0.12882543959165105 1105 7 0.15047519286124356 1105 8 0.22739394938452762 1105 9 0.5243303898812215 1105 10-11 0.16606904047800697 1105 12-13 0.22145754440715848 1105 14-15 0.032063571734148866 1105 16-17 0.06295650234925887 1105 18-19 0.06545933514096447 1105 20-21 0.35461416566317894 1105 22-23 0.39181169993145915 1105 24-25 0.3529188085453896 1105 26-27 0.3060779606642541 1105 28-29 0.27547758847200043 1105 30-31 0.5181867225244972 1105 32-33 0.29524912774491696 1105 34-35 0.2712497556102136 1105 36-37 0.266408670066248 1105 38-39 0.36276002890623005 1105 40-41 0.37905669366821115 1105 42-43 0.3522873469415586 1105 44-45 0.2683965190921569 1105 46-47 0.37200626141748927 1105 48-49 0.1965432166878287 1105 50-51 0.28095677329690716 1105 52-53 0.10415289126300564 1105 54-55 0.1164056901585866 1105 56-57 0.20567055939635281 1105 58-59 0.29964291943436194 1105 60-61 0.190672160022757 1105 62-63 0.28710332139011285 1105 64-65 0.23748669139560263 1105 66-67 0.16622116494995254 1105 68-69 0.16820108298871617 1105 70-71 0.20319687285810062 1105 72-73 0.11772454728488668 1105 74-75 0.10559825145747936 1105 76-77 0.1872753430193157 1105 78-79 0.1748066380156459 1105 80-81 0.08815681468201575 1105 82-83 0.06606370025602004 1105 84-85 0.1863346334726721 1105 86-87 0.18792627557600383 1105 88-89 0.24266321820541847 1105 90-91 0.20629234509657124 1105 92-93 0.15797970180119236 1105 94-95 0.08082590802189316 1105 96-97 0.1813581895943699 1105 98-99 0.11478894718897337 1105 100-101 -0.05705381088817063 1105 102-103 0.03679151958029081 1105 104-105 -0.0039509322808548575 1105 106-107 -0.010245663480745293 1105 108-109 0.03046095893061107 1105 110-111 0.004478042078272182 1105 112-113 0.07958472362864555 1105 114-115 -0.02902763474917336 1105 116-117 -0.0600540040312012 1105 118-119 -0.16391060548684067 1105 120-121 -0.2645645905853726 1105 122-123 -0.18020458968063835 1105 124-125 -0.37354871200864537 1106 1 0.37710218157673125 1106 2 0.312038884869807 1106 3 0.45031145784992077 1106 4 0.27550314481749183 1106 5 0.22203089438298207 1106 6 0.1538366094917123 1106 7 0.19445167728217427 1106 8 0.3778089993551319 1106 9 0.8417319183938616 1106 10-11 0.3189073708718979 1106 12-13 0.4455762980814768 1106 14-15 0.6862563994825308 1106 16-17 0.4873692007619539 1106 18-19 0.3809161252644273 1106 20-21 0.5561756001134981 1106 22-23 0.5538681373212384 1106 24-25 0.5052151048416818 1106 26-27 0.5243824874132272 1106 28-29 0.5527333203943954 1106 30-31 0.612621760737305 1106 32-33 0.6161568290970578 1106 34-35 0.5902538708365483 1106 36-37 0.5092658129233882 1106 38-39 0.622173315208407 1106 40-41 0.5439879105994336 1106 42-43 0.5338534845077021 1106 44-45 0.34024484360714524 1106 46-47 0.4658933866379442 1106 48-49 0.3726455094567882 1106 50-51 0.3605135046314203 1106 52-53 0.24871373782384865 1106 54-55 0.2922657724631108 1106 56-57 0.22324492741516622 1106 58-59 0.38468818692407325 1106 60-61 0.28578209535491794 1106 62-63 0.39727380933837964 1106 64-65 0.38269421638090506 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116-117 -0.8144905112077261 2216 118-119 -0.5742870192461886 2216 120-121 -0.5032601974246163 2216 122-123 -0.4314755952452387 2216 124-125 -0.3727467713147199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32986.0 3 6213.0 4 4051.0 5 3555.0 6 3906.0 7 4564.0 8 5131.0 9 5724.0 10 6414.0 11 7055.0 12 7849.0 13 8056.0 14 8147.0 15 7869.0 16 7132.0 17 6664.0 18 6332.0 19 6194.0 20 6285.0 21 6725.0 22 8034.0 23 10572.0 24 14593.0 25 20335.0 26 24762.0 27 28287.0 28 34063.0 29 40909.0 30 49317.0 31 59851.0 32 75956.0 33 99830.0 34 135951.0 35 198364.0 36 353220.0 37 586690.0 38 232858.0 39 548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.13078845554204 18.57147836473923 12.507736467422994 24.789996712295732 2 15.643111935171156 20.005270637841296 39.51237188114712 24.839245545840434 3 18.623522934225573 24.433620864098184 29.595998098814587 27.34685810286166 4 12.267910438630093 16.14610147891652 37.9942624100325 33.591725672420885 5 13.083217135012958 38.414105872160356 35.227973810318225 13.274703182508462 6 32.67215188443772 37.001219773890064 17.382295584343225 12.944332757328997 7 28.586299229643437 32.200716238664654 21.451912714883363 17.76107181680855 8 26.13885297983451 35.57203476571636 20.1321714643098 18.156940790139327 9 26.00680852826047 15.994535483124093 19.993251707543877 38.005404281071556 10-11 24.572940497105403 26.562166883704556 28.51435485624436 20.350537762945677 12-13 25.097625642719795 23.84950450692109 28.216704926318403 22.83616492404071 14-15 22.19730087010867 25.705595586065648 25.261387910153243 26.835715633672436 16-17 22.704833633262805 28.721274537117214 26.206408262994223 22.367483566625758 18-19 22.665824523817875 27.811469505541503 27.322444620832165 22.200261349808457 20-21 22.977495473984916 27.23130249218523 27.408883721006855 22.382318312823006 22-23 22.865527446723046 27.04746503992227 27.14890909403235 22.93809841932233 24-25 22.80797367459968 27.28059970105309 27.08342746019375 22.82799916415348 26-27 22.51434307751933 27.639682785083153 26.876389391876103 22.969584745521413 28-29 22.658797241026182 27.595997454876773 27.019556222374497 22.72564908172255 30-31 22.82048651134288 27.23060456915699 26.96957174331427 22.97933717618586 32-33 22.49147038753853 27.36923221722864 26.952916539212683 23.186380856020143 34-35 23.10373071412588 27.433489014072443 26.701854639257007 22.76092563254467 36-37 23.16782350135613 26.99844869091678 26.746658026583315 23.08706978114377 38-39 22.61025371943802 27.385463897392214 26.92230768687779 23.081974696291972 40-41 23.05204117815782 27.070890460838935 27.013383536595843 22.863684824407407 42-43 22.664523553887122 26.95961992454869 27.025927476291756 23.349929045272432 44-45 22.73058507427989 27.314344982363277 27.014481854916035 22.9405880884408 46-47 23.10934177144898 27.52615224467649 26.531145748086672 22.833360235787854 48-49 22.734216568878864 27.291291246066173 26.882295552745404 23.09219663230955 50-51 22.79360067014603 27.403678273432757 26.8861034114386 22.916617644982612 52-53 23.01555385137776 27.130327480588573 26.833670338544934 23.020448329488737 54-55 22.70322496692109 27.270431916515264 26.93918399166809 23.08715912489555 56-57 22.679695267229462 27.579280524752185 26.920069417889813 22.820954790128543 58-59 22.712378505129227 27.11110169860448 26.874377156787656 23.30214263947864 60-61 22.49938993870678 27.340276305407624 27.121877892202345 23.038455863683247 62-63 22.547321128534435 27.305507784959836 27.26399678454473 22.883174301960995 64-65 22.458834893656075 27.239975950988963 27.0204757797986 23.28071337555637 66-67 22.460418686521642 27.46404265933714 26.816763462823683 23.258775191317525 68-69 22.63182479759312 27.052360462503106 27.252784749210786 23.06302999069299 70-71 22.605448459642457 27.00393545616584 27.010429841228756 23.380186242962946 72-73 22.584402712031604 27.152155025946534 26.836706743463008 23.426735518558857 74-75 22.55327870877773 27.455675300559385 26.80011474309994 23.190931247562947 76-77 22.747104891247822 27.17594947848746 26.958360413332162 23.11858521693256 78-79 22.789933399330817 27.22485602955721 26.91490160264699 23.070308968464985 80-81 22.91131355693889 27.36861500838809 26.751668499441227 22.968402935231794 82-83 22.870553406192048 27.368873137349038 26.832905029510606 22.927668426948312 84-85 22.83959318402754 27.322743981688152 26.927402330021327 22.910260504262983 86-87 22.877896195387173 27.23099058495287 26.970269461246943 22.920843758413014 88-89 22.84649192166785 27.362973338303455 26.797457142104093 22.993077597924596 90-91 22.77235877390341 27.48382123193721 26.84869530922208 22.895124684937304 92-93 22.93713859995449 27.042000706416268 27.029529000129187 22.991331693500054 94-95 22.72653470378946 27.018340204438925 27.178446377138986 23.07667871463263 96-97 22.90141936127566 26.903976743540305 27.165691921561685 23.02891197362235 98-99 22.848139957672725 27.032346904823868 26.848335734263394 23.27117740324001 100-101 22.637634675549144 27.002905003217975 27.180146673836024 23.179313647396853 102-103 22.623433802913016 26.661748493073965 27.245222723844552 23.469594980168466 104-105 22.346702234027806 27.029696283570008 27.06257005089198 23.5610314315102 106-107 22.608212354863486 26.996262405313015 26.841854848425523 23.55367039139798 108-109 22.626717353921972 26.898042567129398 26.802274526265858 23.672965552682776 110-111 22.580429588431485 27.171131800471652 26.90405676391649 23.34438184718037 112-113 22.668593097557334 27.138297108824126 27.085845792187836 23.107264001430707 114-115 22.541488105225334 27.43426181752976 26.790158116151396 23.23409196109351 116-117 22.431977123294793 27.370131940625424 26.990210522823173 23.20768041325661 118-119 22.37959852301139 27.508570924622315 26.66184209502425 23.449988457342037 120-121 22.45585809444978 27.59341270478213 26.83798203186798 23.11274716890011 122-123 22.484386561805113 27.63889228845839 26.77502245284902 23.10169869688748 124-125 22.450849056603776 27.703301886792453 26.719103773584905 23.126745283018867 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 390.0 1 328.0 2 246.5 3 203.5 4 168.0 5 151.0 6 136.0 7 120.5 8 119.0 9 123.5 10 125.0 11 118.0 12 111.0 13 126.0 14 154.0 15 185.0 16 235.5 17 289.0 18 354.0 19 454.0 20 608.5 21 928.0 22 1383.0 23 2072.0 24 3112.0 25 4585.5 26 6837.0 27 9970.0 28 13266.5 29 17056.5 30 21338.5 31 26330.0 32 33309.0 33 38243.5 34 41350.5 35 46223.0 36 53181.0 37 59598.5 38 63905.0 39 67523.0 40 69882.0 41 73472.5 42 75993.5 43 76858.5 44 79228.0 45 82665.0 46 82754.0 47 80674.5 48 79700.5 49 77598.5 50 75721.0 51 74227.0 52 73728.0 53 74496.0 54 75395.0 55 71326.0 56 64051.0 57 58543.5 58 53837.0 59 46772.0 60 36995.5 61 30192.5 62 25307.5 63 18860.0 64 13549.5 65 10336.5 66 7492.5 67 5927.5 68 4546.5 69 2929.5 70 2036.5 71 1464.0 72 1065.0 73 771.0 74 584.5 75 431.5 76 280.5 77 178.5 78 96.0 79 53.0 80 49.0 81 31.0 82 17.5 83 16.5 84 13.5 85 11.5 86 14.0 87 11.5 88 8.0 89 6.5 90 3.5 91 3.0 92 2.5 93 2.5 94 3.0 95 2.0 96 1.5 97 1.5 98 1.5 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09082387133692738 2 5.647080083125019E-4 3 9.411800138541698E-5 4 0.0027294220401770926 5 0.0015529470228593802 6 3.764720055416679E-4 7 9.411800138541698E-5 8 0.0011764750173177121 9 4.7059000692708494E-4 10-11 0.008047089118453152 12-13 0.0017882420263229226 14-15 0.004000015058880221 16-17 0.0022588320332500075 18-19 0.011929456675601602 20-21 0.015552999728940156 22-23 0.00825885462157034 24-25 0.009411800138541697 26-27 0.008776503629190134 28-29 0.006776496099750023 30-31 0.016705945245911517 32-33 0.0019764780290937563 34-35 0.0011058865162786495 36-37 5.882375086588561E-4 38-39 3.294130048489594E-4 40-41 0.001435299521127609 42-43 0.002141184531518236 44-45 0.001058827515585941 46-47 0.0014588290214739633 48-49 0.0072000271059843995 50-51 0.0038588380568020963 52-53 0.00698826160286721 54-55 0.00698826160286721 56-57 0.008447090624341175 58-59 0.007505910610487003 60-61 0.009670624642351595 62-63 0.011952986175947956 64-65 0.007741205613950546 66-67 0.01185886817456254 68-69 0.011741220672830769 70-71 0.003741190555070325 72-73 0.01120004216486462 74-75 0.011552984670059935 76-77 0.005411785079661477 78-79 0.007694146613257838 80-81 0.011929456675601602 82-83 0.015623588229979219 84-85 0.011647102671445352 86-87 0.014494172213354214 88-89 0.013929464205041714 90-91 0.010188273649971387 92-93 0.008588267626419299 94-95 0.00715296810529169 96-97 0.007670617112911483 98-99 0.005694139083817728 100-101 0.00985886064512243 102-103 0.010329450652049514 104-105 0.009294152636809928 106-107 0.004517664066500015 108-109 0.0031058940457187605 110-111 0.005694139083817728 112-113 0.007741205613950546 114-115 0.012070633677679727 116-117 0.009905919645815137 118-119 0.21630669668403457 120-121 0.02392950185224227 122-123 0.025411860374062588 124-125 0.2349185314580008 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2124992.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.74664991452269 #Duplication Level Percentage of deduplicated Percentage of total 1 86.78790647626776 63.13529449243387 2 8.291854384625118 12.06409256121047 3 2.3518694026712974 5.132718602424194 4 0.9273103599215929 2.6983488846130466 5 0.4453997073558562 1.6200668291523663 6 0.26947250704718945 1.1761933279050385 7 0.18182632694941228 0.9259079306282706 8 0.1117478999524934 0.6503428285221715 9 0.08027708814999086 0.5255900305024197 >10 0.4807838913143881 6.71031752944151 >50 0.046916101862810033 2.3626950927485564 >100 0.024495877504393138 2.9434447026846833 >500 1.3997637767773792E-4 0.05498718773341721 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4117700207812547E-4 2 9.411800138541698E-5 0.0 0.0 0.0 1.4117700207812547E-4 3 9.411800138541698E-5 0.0 0.0 0.0 1.4117700207812547E-4 4 9.411800138541698E-5 0.0 0.0 0.0 1.4117700207812547E-4 5 9.411800138541698E-5 0.0 0.0 4.705900069270849E-5 1.4117700207812547E-4 6 9.411800138541698E-5 0.0 0.0 4.705900069270849E-5 1.4117700207812547E-4 7 9.411800138541698E-5 0.0 0.0 4.705900069270849E-5 1.4117700207812547E-4 8 9.411800138541698E-5 0.0 0.0 4.705900069270849E-5 1.4117700207812547E-4 9 9.411800138541698E-5 0.0 0.0 4.705900069270849E-5 1.4117700207812547E-4 10-11 9.411800138541698E-5 2.3529500346354244E-5 0.0 4.705900069270849E-5 1.4117700207812547E-4 12-13 1.8823600277083396E-4 4.705900069270849E-5 0.0 4.705900069270849E-5 2.3529500346354244E-4 14-15 2.3529500346354244E-4 7.058850103906273E-5 0.0 4.705900069270849E-5 2.8235400415625093E-4 16-17 2.3529500346354244E-4 9.411800138541698E-5 0.0 7.058850103906273E-5 3.764720055416679E-4 18-19 3.058835045026052E-4 9.411800138541698E-5 0.0 9.411800138541698E-5 3.764720055416679E-4 20-21 3.294130048489594E-4 9.411800138541698E-5 0.0 1.4117700207812547E-4 3.764720055416679E-4 22-23 3.294130048489594E-4 9.411800138541698E-5 0.0 3.058835045026052E-4 3.764720055416679E-4 24-25 5.176490076197934E-4 9.411800138541698E-5 0.0 4.0000150588802215E-4 3.764720055416679E-4 26-27 5.176490076197934E-4 1.4117700207812547E-4 0.0 0.0010352980152395868 3.764720055416679E-4 28-29 6.117670090052104E-4 1.4117700207812547E-4 0.0 0.002964717043640635 4.0000150588802215E-4 30-31 7.058850103906273E-4 2.3529500346354244E-4 0.0 0.01009415564858597 4.4706050658073064E-4 32-33 7.058850103906273E-4 3.294130048489594E-4 0.0 0.025576566876487065 4.705900069270849E-4 34-35 7.7647351142969E-4 3.294130048489594E-4 0.0 0.04863547721591423 4.705900069270849E-4 36-37 9.882390145468784E-4 3.294130048489594E-4 0.0 0.09042386983103937 4.705900069270849E-4 38-39 0.0010823570159322954 4.235310062343764E-4 0.0 0.15508293678282084 4.705900069270849E-4 40-41 0.0010823570159322954 4.235310062343764E-4 0.0 0.2225655437761648 4.705900069270849E-4 42-43 0.001152945516971358 4.4706050658073064E-4 0.0 0.2902363867722796 4.705900069270849E-4 44-45 0.0013176520193958377 4.705900069270849E-4 0.0 0.377272008553444 4.705900069270849E-4 46-47 0.0014117700207812549 4.705900069270849E-4 0.0 0.4791076860524651 4.705900069270849E-4 48-49 0.0014588290214739633 4.705900069270849E-4 0.0 0.5861433831280306 4.705900069270849E-4 50-51 0.0014588290214739633 4.705900069270849E-4 0.0 0.7057438333885493 5.647080083125019E-4 52-53 0.0014823585218203175 4.705900069270849E-4 0.0 0.8169913110261121 5.647080083125019E-4 54-55 0.0015058880221666716 4.705900069270849E-4 0.0 0.9378388248049876 6.588260096979188E-4 56-57 0.0016000060235520886 4.705900069270849E-4 0.0 1.0878393895129959 6.588260096979188E-4 58-59 0.0016000060235520886 5.882375086588561E-4 0.0 1.254498840466223 6.588260096979188E-4 60-61 0.0016470650242447972 6.117670090052104E-4 0.0 1.4300289130500254 6.588260096979188E-4 62-63 0.0016470650242447972 6.117670090052104E-4 0.0 1.5992530795410054 8.000030117760443E-4 64-65 0.0016470650242447972 6.588260096979188E-4 0.0 1.777512574164985 8.000030117760443E-4 66-67 0.0018353010270156312 6.588260096979188E-4 0.0 1.9819604026744573 8.000030117760443E-4 68-69 0.0018353010270156312 6.588260096979188E-4 0.0 2.2138200990874317 8.000030117760443E-4 70-71 0.0018353010270156312 6.588260096979188E-4 0.0 2.4694445908502245 8.000030117760443E-4 72-73 0.0018823600277083396 6.588260096979188E-4 0.0 2.734033822244978 8.70591512815107E-4 74-75 0.0019294190284010481 6.588260096979188E-4 0.0 2.994458332078427 9.882390145468782E-4 76-77 0.0019294190284010481 6.588260096979188E-4 0.0 3.273447617685149 0.0010352980152395868 78-79 0.0019294190284010481 6.588260096979188E-4 0.0 3.5929076438876004 0.0010352980152395868 80-81 0.002305891033942716 6.588260096979188E-4 0.0 3.9415442505195317 0.0010352980152395868 82-83 0.0023529500346354247 6.82355510044273E-4 0.0 4.305286796373821 0.0010823570159322954 84-85 0.002400009035328133 7.058850103906273E-4 0.0 4.658299890070174 0.0011294160166250037 86-87 0.0024705975363671956 7.058850103906273E-4 0.0 5.0277836340089745 0.0011764750173177121 88-89 0.00249412703671355 7.058850103906273E-4 0.0 5.423243946330151 0.0012235340180104207 90-91 0.002588245038098967 7.529440110833358E-4 0.0 5.861316183778574 0.0012470635183567751 92-93 0.002588245038098967 7.529440110833358E-4 0.0 6.317976726500618 0.0013176520193958377 94-95 0.002588245038098967 7.529440110833358E-4 0.0 6.779390228292624 0.0013176520193958377 96-97 0.002588245038098967 7.529440110833358E-4 0.0 7.242380206607836 0.0014823585218203175 98-99 0.0027294220401770926 7.529440110833358E-4 0.0 7.7276055627503535 0.0015529470228593802 100-101 0.0028941285426015723 7.529440110833358E-4 0.0 8.270619371743518 0.0015529470228593802 102-103 0.0030117760443333433 7.529440110833358E-4 0.0 8.848009780742704 0.0015529470228593802 104-105 0.003341189049182303 7.529440110833358E-4 0.0 9.427870787278259 0.0015529470228593802 106-107 0.0034588365509140738 7.529440110833358E-4 0.0 10.00587296328645 0.0015529470228593802 108-109 0.0034823660512604284 7.529440110833358E-4 0.0 10.601781089058218 0.0016000060235520886 110-111 0.0035058955516067826 7.7647351142969E-4 0.0 11.241336437972471 0.0016470650242447972 112-113 0.003552954552299491 8.000030117760443E-4 0.0 11.925456660542721 0.0016470650242447972 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2330 0.0 42.922607 1 ATCAACG 2200 0.0 41.90083 3 CAACGCA 2285 0.0 40.08754 5 TCAACGC 2365 0.0 38.978428 4 AACGCAG 2550 0.0 36.388096 6 GTACATG 6195 0.0 34.881737 1 TACATGG 6390 0.0 33.50543 2 GGTATCA 1550 0.0 33.029312 1 TATCAAC 2940 0.0 32.365788 2 ACATGGG 6270 0.0 32.344494 3 ACGCAGA 3095 0.0 29.788313 7 CGCAGAG 3160 0.0 28.610891 8 CATGGGG 4335 0.0 27.575966 4 TAAGGTG 1260 0.0 26.907862 5 GTACCGT 145 1.3435785E-4 24.612637 6 GTAAGGT 1210 0.0 23.59281 4 GGTAAGG 1260 0.0 22.656052 3 GAGTACA 4785 0.0 22.26916 1 GTATAGA 830 0.0 20.799488 1 GTGTAAG 1130 0.0 20.545605 1 >>END_MODULE