FastQCFastQC Report
Thu 26 May 2016
SRR1364420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124992
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.063.2560121
GTATCAA41600.052.99821
ATCAACG49900.043.8050083
TCAACGC50750.042.952964
CAACGCA51650.042.3961835
AACGCAG53800.040.9183046
TATCAAC54350.039.780992
ACGCAGA63550.034.453277
CGCAGAG64700.033.9312448
GTACATG59650.033.464711
TACATGG61300.032.2586442
ACATGGG61200.032.018633
CATGGGG40450.028.4175624
GCAGAGT81800.026.401659
AGAGTAC72950.023.73260310-11
CAGAGTA74500.023.63813210-11
GAGTACT53100.023.02475412-13
GAGTACA45250.022.781391
GTACTTT54200.022.44769314-15
GGACCGT2607.704566E-720.5952636
Produced by FastQC (version 0.11.5)