FastQCFastQC Report
Thu 26 May 2016
SRR1364419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609881
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.040.04761
GTATCAA27700.039.7584461
GTACATG80850.038.1405681
TACATGG86900.035.588782
TCAACGC29450.034.938054
ACATGGG87950.033.946693
ATCAACG30650.033.569523
CAACGCA31300.032.876175
AACGCAG32750.031.6009966
CATGGGG63500.028.9416054
TATCAAC37250.028.8989332
ACGCAGA39200.026.097887
TATACCG1951.4700436E-624.4025295
CGCAGAG42200.024.3835248
CATGGGA40100.023.5825424
GAGTACA64000.022.788911
AGTACAT62450.021.5231322
ATGGGGA38700.019.8270535
CAGAGTA50150.018.264710-11
ATTCGCG4600.018.1067880-81
Produced by FastQC (version 0.11.5)