FastQCFastQC Report
Thu 26 May 2016
SRR1364419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609881
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27350.057.957681
GTATCAA41400.047.7886121
TCAACGC49750.039.1448554
ATCAACG50350.038.6783873
GTACATG80350.037.0826531
CAACGCA53300.036.506145
TATCAAC55550.035.597192
AACGCAG54700.035.5690776
TACATGG87350.033.6841622
ACATGGG88400.032.1355823
ACGCAGA64850.029.910237
CGCAGAG68450.028.3349888
CATGGGG65400.027.5010494
GCAGAGT83200.023.3116579
GAGTACA66900.022.3580681
ATGGGGA34450.021.5906755
CAGAGTA80450.021.52017610-11
AGTACAT64650.021.2816452
GAGTACT44950.020.31690612-13
AGAGTAC79350.019.94406110-11
Produced by FastQC (version 0.11.5)