FastQCFastQC Report
Thu 26 May 2016
SRR1364418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792499
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.063.5339161
GTATCAA35350.049.840991
TCAACGC40800.039.9436264
GTACATG114300.039.734031
ATCAACG41400.039.218883
CAACGCA42050.038.7584045
TACATGG117150.037.7714352
AACGCAG44850.036.3387036
TATCAAC47500.035.6848682
ACATGGG120600.035.2607543
CATGGGA90750.031.525034
CGCAGAG55100.029.2549278
CATGGGG31950.029.040954
ACGCAGA55650.028.9657927
ATGGGAT38650.027.5476685
GAGTACA80550.025.8634741
AGTACAT82650.024.4663352
GCAGAGT65400.024.1934249
GGACCGA3252.3646862E-1123.7925686
ATGGGAG39050.023.305145
Produced by FastQC (version 0.11.5)